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\u7ec6\u80de\u540d\u79f0\uff1aBHK-21 [C-13] \u4ed3\u9f20\u80be\u6210\u7ea4\u7ef4\u7ec6\u80de
\u7ec6\u80de\u522b\u79f0\uff1aBHK 21; BHK21; Baby Hamster Kidney-21; Baby Hamster Kidney 21; Baby Hamster Kidney from litter No. 21; BHK\uff1b\u4ed3\u9f20\u80be\u6210\u7ea4\u7ef4\u7ec6\u80de
\u79cd\u5c5e\u6765\u6e90\uff1a\u4ed3\u9f20\uff1b1 \u65e5\u9f84
\u7ec4\u7ec7\u6765\u6e90\uff1a\u6b63\u5e38\u80be
\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f
\u7ec6\u80de\u5f62\u6001\uff1a\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837
\u500d\u589e\u65f6\u95f4\uff1a~12 hours
\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185
\u80cc\u666f\u4ecb\u7ecd\uff1aBHK-21 \u7ec6\u80de\u7cfb\uff08Baby hamster kidney cell line\uff09\u662f\u4ece\u53d9\u5229\u4e9a\u5e7c\u9f20\u80be\u7ec4\u7ec7\u4e2d\u5206\u79bb\u57f9\u517b\u83b7\u5f97\u7684\u6210\u7ea4\u7ef4\u578b\u8d34\u58c1\u7ec6\u80de\u7cfb\uff0c\u5176\u53ef\u8fde\u7eed\u4f20\u4ee3\u3002BHK-21 \u7ec6\u80de\u7cfb\u53ef\u4ee5\u7528\u4e8e\u591a\u79cd\u75c5\u6bd2\u7684\u589e\u6b96\u548c\u7eaf\u5316\uff0c\u5982\u53e3\u8e44\u75ab\u75c5\u6bd2\uff08Foot-and-mouth disease virus\uff0cFMDV\uff09\u3001\u8111\u5fc3\u808c\u708e\u75c5\u6bd2\uff08Encephalomyocarditis virus\uff0cEMCV\uff09\u3001\u547c\u80a0\u5b64\u75c5\u6bd2\uff08Reovirus\uff09\u3001\u6c34\u75b1\u6027\u53e3\u708e\u75c5\u6bd2\uff08Vesicularstomatitis virus\uff0cVSV\uff09\u7b49\u3002\u76ee\u524d\uff0cBHK-21 \u7ec6\u80de\u7cfb\u5df2\u88ab\u5e7f\u6cdb\u5e94\u7528\u4e8e\u53e3\u8e44\u75ab\u75ab\u82d7\u7684\u751f\u4ea7
\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1
\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5
\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0
\u4fdd\u85cf\u673a\u6784\uff1aATCC; CRL-6282
\u57f9\u517b\u57fa\uff1a90% MEM \u57f9\u517b\u57fa + 10% FBS\uff08\u80ce\u725b\u8840\u6e05\uff09+ PS\uff08\u9752\u9709\u7d20 – \u94fe\u9709\u7d20\u53cc\u6297\uff09
\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8 95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c\u6e29\u5ea6 37\u2103
\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58
\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3
\u53d1\u8d27\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09\uff0c\u987a\u4e30\u5feb\u9012
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