{"id":1325,"date":"2025-10-03T13:16:41","date_gmt":"2025-10-03T05:16:41","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&p=1325"},"modified":"2025-10-03T13:16:42","modified_gmt":"2025-10-03T05:16:42","slug":"msc-fluc-egfp-puro%e4%ba%ba%e8%84%90%e5%b8%a6%e9%97%b4%e5%85%85%e8%b4%a8%e5%b9%b2%e7%bb%86%e8%83%9efluc-egfp%e5%8f%8c%e6%a0%87","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/msc-fluc-egfp-puro%e4%ba%ba%e8%84%90%e5%b8%a6%e9%97%b4%e5%85%85%e8%b4%a8%e5%b9%b2%e7%bb%86%e8%83%9efluc-egfp%e5%8f%8c%e6%a0%87\/","title":{"rendered":"MSC-FLUC-eGFP-puro\u4eba\u8110\u5e26\u95f4\u5145\u8d28\u5e72\u7ec6\u80de(FLUC\/eGFP\u53cc\u6807)"},"content":{"rendered":"

\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n
\u7ec6\u80de\u540d\u79f0\uff1aMSC-FLUC-eGFP-puro \u4eba\u8110\u5e26\u95f4\u5145\u8d28\u5e72\u7ec6\u80de (FLUC\/eGFP \u53cc\u6807)<\/p>\n
<\/div>\n

\u7ec6\u80de\u522b\u79f0\uff1aMSC-EGFP-LUC\uff1b\u4eba\u8110\u5e26\u95f4\u5145\u8d28\u5e72\u7ec6\u80de – \u7eff\u8272\u8367\u5149\u86cb\u767d – \u8367\u5149\u7d20\u9176\u6807\u8bb0\uff1b\u4eba\u8110\u5e26\u95f4\u5145\u8d28\u5e72\u7ec6\u80de – EGFP-LUC<\/p>\n

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\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/p>\n

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\u7ec4\u7ec7\u6765\u6e90\uff1a\u8110\u5e26<\/p>\n

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\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f<\/p>\n

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\u5f62\u6001\u7279\u5f81\uff1a\u68ad\u5f62\u7ec6\u80de\u6837<\/p>\n

<\/div>\n

\u7ec6\u80de\u4ee3\u6570\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff0c\u5efa\u8bae\u4f18\u5148\u9009\u62e9\u4f4e\u4f20\u4ee3\u7ec6\u80de\uff08\u5982 p3-p10\uff09\uff0c\u4ee5\u7ef4\u6301\u53cc\u8367\u5149\u6807\u8bb0\u7a33\u5b9a\u6027\u3001\u591a\u5411\u5206\u5316\u80fd\u529b\u53ca\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u6838\u5fc3\u751f\u7269\u5b66\u7279\u6027\u3002<\/p><\/div>\n

\u80cc\u666f\u4e0e\u7b5b\u9009\u9a8c\u8bc1<\/h2>\n
\u80cc\u666f\u4ecb\u7ecd\uff1a<\/p>\n
<\/div>\n

MSC-FLUC-eGFP-puro \u7ec6\u80de\u7a33\u5b9a\u5171\u8868\u8fbe\u8424\u706b\u866b\u8367\u5149\u7d20\u9176\uff08FLUC\uff09\u4e0e\u589e\u5f3a\u578b\u7eff\u8272\u8367\u5149\u86cb\u767d\uff08eGFP\uff09\uff0c\u6027\u72b6\u7a33\u5b9a\uff0c\u57f9\u517b\u65f6\u65e0\u9700\u6297\u751f\u7d20\u5e38\u89c4\u7ef4\u6301\u3002\u65e2\u53ef\u4f5c\u4e3a\u53cc\u8367\u5149\u6d3b\u6027\u68c0\u6d4b\u7684\u9633\u6027\u5bf9\u7167\uff0c\u4e5f\u9002\u7528\u4e8e\u5e72\u7ec6\u80de\u79fb\u690d\u3001\u5f52\u5de2\u8ffd\u8e2a\u7b49\u6d3b\u4f53\u52a8\u7269\u6210\u50cf\u5b9e\u9a8c\u3002<\/p>\n

<\/div>\n

\u8be5\u7ec6\u80de\u901a\u8fc7\u6162\u75c5\u6bd2\u8f6c\u67d3\u643a\u5e26 Luc \u57fa\u56e0\uff0c\u4eb2\u672c\u8110\u5e26\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u5177\u5907\u591a\u5411\u5206\u5316\u80fd\u529b\uff0c\u53ef\u5206\u5316\u4e3a\u9aa8\u7ec6\u80de\u3001\u8102\u80aa\u7ec6\u80de\u548c\u8f6f\u9aa8\u7ec6\u80de\uff0c\u4e14\u80fd\u53c2\u4e0e\u9020\u8840\u5fae\u73af\u5883\u6784\u6210\uff0c\u5e7f\u6cdb\u5e94\u7528\u4e8e\u7ec4\u7ec7\u5de5\u7a0b\u3001\u7ec6\u80de\u6cbb\u7597\u53ca\u57fa\u56e0\u6cbb\u7597\u76f8\u5173\u7814\u7a76\u3002<\/p><\/div>\n

<\/div>\n
puro \u836f\u7b5b\u6d53\u5ea6\uff1a<\/p>\n
<\/div>\n

MSC \u7ec6\u80de puro\uff08\u560c\u5464\u9709\u7d20\uff09\u836f\u7b5b\u6d53\u5ea6\u4e3a 1.0\u03bcg\/ml\uff0c\u7528\u4e8e\u53bb\u9664\u672a\u8f6c\u67d3\u7ec6\u80de\uff1b\u57f9\u517b\u8fc7\u7a0b\u4e2d\u5efa\u8bae\u4f7f\u7528 0.5\u03bcg\/ml \u6d53\u5ea6 puro \u7ef4\u6301\u3002\u4f4e\u6d53\u5ea6\u7ef4\u6301\u53ef\u4fdd\u969c FLUC \u4e0e eGFP \u53cc\u57fa\u56e0\u7a33\u5b9a\u8868\u8fbe\uff0c\u540c\u65f6\u4e0d\u5f71\u54cd\u7ec6\u80de\u8d34\u58c1\u751f\u957f\u3001\u589e\u6b96\u80fd\u529b\u53ca\u591a\u5411\u5206\u5316\u7279\u6027\u3002<\/p><\/div>\n

<\/div>\n

\u7ec6\u80de\u57f9\u517b\u6838\u5fc3\u4fe1\u606f<\/h2>\n
\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff0c\u53c2\u8003\u540c\u7c7b\u4eba\u6e90\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u6807\u51c6\uff0c\u5efa\u8bae\u6309 1 \u7ea7\u751f\u7269\u5b89\u5168\u9632\u62a4\u64cd\u4f5c\uff08\u5e38\u89c4\u5b9e\u9a8c\u5ba4\u73af\u5883\uff0c\u64cd\u4f5c\u65f6\u4f69\u6234\u624b\u5957\u3001\u53e3\u7f69\uff0c\u907f\u514d\u7ec6\u80de\u6c61\u67d3\u6216\u57f9\u517b\u6db2\u63a5\u89e6\u76ae\u80a4\uff09\uff0c\u5177\u4f53\u53ef\u53c2\u7167\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u8865\u5145\u786e\u8ba4\u3002<\/p>\n
<\/div>\n

\u4ea7\u54c1\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u7ec6\u80de\u57f9\u517b\u74f6\u3002<\/p>\n

<\/div>\n

\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff0c\u53ef\u901a\u8fc7\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u6216\u4f9b\u5e94\u5546\u83b7\u53d6\u8be6\u7ec6\u6eaf\u6e90\u4fe1\u606f\uff0c\u786e\u4fdd\u7ec6\u80de\u682a\u591a\u5411\u5206\u5316\u80fd\u529b\u4e0e\u53cc\u8367\u5149\u7279\u6027\u7a33\u5b9a\u3002<\/p>\n

<\/div>\n

\u57f9\u517b\u57fa\uff1aMSC-EGFP-LUC \u4eba\u8110\u5e26\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u4e13\u7528\u57f9\u517b\u57fa\uff08IML-099-1\uff09\uff0c\u9700\u4f7f\u7528\u4e13\u7528\u914d\u65b9\uff0c\u4e0d\u53ef\u968f\u610f\u66ff\u6362\uff0c\u4ee5\u7ef4\u6301\u7ec6\u80de\u68ad\u5f62\u5f62\u6001\u3001\u589e\u6b96\u80fd\u529b\u53ca\u53cc\u8367\u5149\u8868\u8fbe\u3002<\/p>\n

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\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8 95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c\u6e29\u5ea6 37\u2103\uff08\u5e38\u89c4 CO\u2082\u57f9\u517b\u73af\u5883\u5373\u53ef\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff1b\u907f\u514d\u5f3a\u5149\u76f4\u5c04\uff0c\u51cf\u5c11 eGFP \u5149\u6f02\u767d\uff09\u3002<\/p>\n

<\/div>\n

\u4f20\u4ee3\u65b9\u6cd5\uff1a\u6309 1:3 \u81f3 1:5 \u6bd4\u4f8b\u4f20\u4ee3\uff0c\u6bcf\u5468 2-3 \u6b21\uff1b\u4f20\u4ee3\u65f6\u52a8\u4f5c\u8f7b\u67d4\uff0c\u907f\u514d\u8fc7\u5ea6\u6d88\u5316\u635f\u4f24\u7ec6\u80de\uff0c\u4fdd\u969c\u4f20\u4ee3\u540e\u7ec6\u80de\u8d34\u58c1\u4e0e\u589e\u6b96\u6548\u7387\u3002<\/p>\n

<\/div>\n

\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff08\u5efa\u8bae\u4f7f\u7528\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u4e13\u7528\u51bb\u5b58\u6db2\uff09\uff0c\u6db2\u6c2e\u50a8\u5b58\uff1b\u51bb\u5b58\u524d\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff0c\u53ef\u9002\u5f53\u63d0\u9ad8\u51bb\u5b58\u5bc6\u5ea6\uff0c\u51cf\u5c11\u590d\u82cf\u635f\u4f24\uff0c\u4fdd\u7559\u591a\u5411\u5206\u5316\u80fd\u529b\u4e0e\u53cc\u8367\u5149\u7279\u6027\u3002<\/p>\n

<\/div>\n

\u4ea7\u54c1\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\u3002<\/p>\n

<\/div>\n

\u8fd0\u8f93\u65b9\u5f0f\uff1aT25 \u57f9\u517b\u74f6\u5305\u88c5\uff0c\u987a\u4e30\u5feb\u9012\uff08\u5305\u90ae\uff09\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u9700\u5c3d\u91cf\u51cf\u5c11\u98a0\u7c38\uff0c\u964d\u4f4e\u7ec6\u80de\u8131\u843d\u6982\u7387\u3002<\/p>\n

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\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u91cd\u590d\u6807\u6ce8\uff0c\u9700\u4e25\u683c\u9075\u5b88\uff09\u3002<\/p><\/div>\n

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\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff0c\u4e14\u9700\u4e25\u683c\u4f7f\u7528\u4e13\u7528\u57f9\u517b\u57fa\u4e0e\u89c4\u8303\u4f20\u4ee3\u65b9\u6cd5\u3002<\/div>\n","protected":false},"excerpt":{"rendered":"

\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aMSC-FLUC-eGFP-puro \u4eba\u8110\u5e26<\/p>\n

\n \u7ee7\u7eed\u9605\u8bfb <\/span><\/i><\/a>\n <\/div>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[18],"product_tag":[],"class_list":{"0":"post-1325","1":"product","2":"type-product","3":"status-publish","5":"product_cat-18","7":"first","8":"instock","9":"sale","10":"taxable","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product\/1325","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/comments?post=1325"}],"wp:attachment":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/media?parent=1325"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=1325"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=1325"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=1325"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}