{"id":1339,"date":"2025-10-03T16:56:51","date_gmt":"2025-10-03T08:56:51","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&p=1339"},"modified":"2025-10-03T16:56:51","modified_gmt":"2025-10-03T08:56:51","slug":"calu-1-fluc-egfp-puro%e4%ba%ba%e8%82%ba%e7%99%8c%e7%bb%86%e8%83%9efluc-egfp%e5%8f%8c%e6%a0%87","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/calu-1-fluc-egfp-puro%e4%ba%ba%e8%82%ba%e7%99%8c%e7%bb%86%e8%83%9efluc-egfp%e5%8f%8c%e6%a0%87\/","title":{"rendered":"calu-1-FLUC-eGFP-puro\u4eba\u80ba\u764c\u7ec6\u80de(FLUC\/eGFP\u53cc\u6807)"},"content":{"rendered":"

\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n
\u7ec6\u80de\u540d\u79f0\uff1acalu-1-FLUC-eGFP-puro \u4eba\u80ba\u764c\u7ec6\u80de (FLUC\/eGFP \u53cc\u6807)(STR \u9274\u5b9a\u6b63\u786e)<\/p>\n
<\/div>\n

\u7ec6\u80de\u522b\u79f0\uff1aCalu-1-LUC-EGFP\uff1b\u4eba\u80ba\u764c\u7ec6\u80de – \u7eff\u8272\u8367\u5149\u86cb\u767d – \u8367\u5149\u7d20\u9176\u6807\u8bb0\uff1bCalu-1 – \u7eff\u8272\u8367\u5149\u86cb\u767d – \u8367\u5149\u7d20\u9176\u6807\u8bb0<\/p>\n

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\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/p>\n

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\u5e74\u9f84\u6027\u522b\uff1a\u7537\uff1b47 \u5c81<\/p>\n

<\/div>\n

\u7ec4\u7ec7\u6765\u6e90\uff1a\u5668\u5b98\uff1a\u80ba\uff1b\u80bf\u7624\u5206\u671f\uff1a\u2162 \u671f\uff1b\u75be\u75c5\uff1a\u8868\u76ae\u7624\uff1b\u53d6\u6750\u8f6c\u79fb\u7076\uff1a\u808b\u819c<\/p>\n

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\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f<\/p>\n

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\u7ec6\u80de\u5f62\u6001\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837<\/p>\n

<\/div>\n

\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4f20\u4ee3\u8303\u56f4\uff0c\u53ef\u66f4\u597d\u7ef4\u6301 FLUC\/eGFP \u53cc\u8367\u5149\u6807\u8bb0\u7a33\u5b9a\u6027\u53ca\u80ba\u764c \u2162 \u671f\u808b\u819c\u8f6c\u79fb\u76f8\u5173\u751f\u7269\u5b66\u7279\u5f81\uff09<\/p><\/div>\n

\u80cc\u666f\u4e0e\u7b5b\u9009\u9a8c\u8bc1<\/h2>\n
\u80cc\u666f\u4ecb\u7ecd\uff1acalu-1-FLUC-eGFP-puro \u7ec6\u80de\u7a33\u5b9a\u5171\u8868\u8fbe\u8424\u706b\u866b\u8367\u5149\u7d20\u9176\uff08FLUC\uff09\u4e0e\u589e\u5f3a\u578b\u7eff\u8272\u8367\u5149\u86cb\u767d\uff08eGFP\uff09\uff0c\u6027\u72b6\u7a33\u5b9a\uff0c\u53ef\u7528\u4f5c\u53cc\u8367\u5149\u6d3b\u6027\u68c0\u6d4b\u7684\u9633\u6027\u5bf9\u7167\uff0c\u4e5f\u9002\u7528\u4e8e\u80ba\u764c \u2162 \u671f\u808b\u819c\u8f6c\u79fb\u76f8\u5173\u6d3b\u4f53\u52a8\u7269\u6210\u50cf\u5b9e\u9a8c\uff08\u5982\u8f6c\u79fb\u6a21\u578b\u6784\u5efa\u3001\u8f6c\u79fb\u6291\u5236\u836f\u7269\u6548\u679c\u8bc4\u4f30\u7684\u8367\u5149\u8ffd\u8e2a\uff09\u3002\u8be5\u7ec6\u80de\u6e90\u81ea\u80ba\u764c \u2162 \u671f\u808b\u819c\u8f6c\u79fb\u7076\uff0c\u4fdd\u7559\u8868\u76ae\u7624\u7279\u6027\u4e0e\u808b\u819c\u8f6c\u79fb\u6838\u5fc3\u7279\u5f81\uff0c\u662f\u7814\u7a76\u665a\u671f\u80ba\u764c\u8f6c\u79fb\u673a\u5236\u7684\u4e13\u7528\u7ec6\u80de\u682a\u3002<\/p>\n
<\/div>\n

puro \u836f\u7b5b\u6d53\u5ea6\uff1aCalu-1-LUC-EGFP \u7ec6\u80de puro\uff08\u560c\u5464\u9709\u7d20\uff09\u836f\u7b5b\u6d53\u5ea6\u4e3a 1.0\u03bcg\/ml\uff0c\u7528\u4e8e\u53bb\u9664\u672a\u8f6c\u67d3\u7ec6\u80de\uff1b\u57f9\u517b\u8fc7\u7a0b\u4e2d\u5efa\u8bae\u4f7f\u7528 0.5\u03bcg\/ml \u6d53\u5ea6 puro \u7ef4\u6301\u3002\u4f4e\u6d53\u5ea6\u7ef4\u6301\u53ef\u4fdd\u969c\u53cc\u8367\u5149\u57fa\u56e0\u7a33\u5b9a\u8868\u8fbe\uff0c\u540c\u65f6\u4e0d\u5f71\u54cd\u7ec6\u80de\u8d34\u58c1\u751f\u957f\u53ca\u80ba\u764c \u2162 \u671f\u808b\u819c\u8f6c\u79fb\u76f8\u5173\u7279\u6027\u3002<\/p>\n

<\/div>\n

\u7ec6\u80de\u57f9\u517b\u6838\u5fc3\u4fe1\u606f<\/p><\/div>\n

\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1 \u7ea7\uff0c\u64cd\u4f5c\u98ce\u9669\u4f4e\uff0c\u7b26\u5408\u5e38\u89c4\u5b9e\u9a8c\u5ba4\u751f\u7269\u5b89\u5168\u7ba1\u7406\u8981\u6c42\uff0c\u65e0\u9700\u7279\u6b8a\u9632\u62a4\u3002<\/p>\n
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\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\u3002<\/p>\n

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\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff0c\u907f\u514d\u5e72\u6270\u7ec6\u80de\u589e\u6b96\u3001\u53cc\u8367\u5149\u8868\u8fbe\u53ca\u80ba\u764c\u665a\u671f\u8f6c\u79fb\u76f8\u5173\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u6570\u636e\u51c6\u786e\u6027\uff09\u3002<\/p>\n

<\/div>\n

\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff0c\u53ef\u901a\u8fc7\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u6216\u4f9b\u5e94\u5546\u83b7\u53d6\u8be6\u7ec6\u6eaf\u6e90\u4fe1\u606f\uff0c\u786e\u4fdd\u7ec6\u80de\u682a\u80ba\u764c \u2162 \u671f\u5c5e\u6027\u3001\u808b\u819c\u8f6c\u79fb\u7279\u6027\u53ca\u53cc\u8367\u5149\u6807\u8bb0\u7a33\u5b9a\u6027\u3002<\/p>\n

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\u57f9\u517b\u57fa\uff1a89% McCoy’s 5A \u57f9\u517b\u57fa + 10% FBS\uff08\u80ce\u725b\u8840\u6e05\uff09+1% PS\uff08\u9752\u9709\u7d20 – \u94fe\u9709\u7d20\u53cc\u6297\uff09+1.0\u03bcg\/ml puro\uff08\u4ec5\u836f\u7b5b\u9636\u6bb5\u6309\u6b64\u6d53\u5ea6\u6dfb\u52a0\uff0c\u5e38\u89c4\u57f9\u517b\u5efa\u8bae\u7528 0.5\u03bcg\/ml puro \u7ef4\u6301\uff1b\u4e13\u7528 McCoy’s 5A \u57f9\u517b\u57fa\u9002\u914d\u8be5\u7ec6\u80de\u751f\u957f\u9700\u6c42\uff0c\u4e0d\u53ef\u968f\u610f\u66ff\u6362\uff09\u3002<\/p>\n

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\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8 95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c\u6e29\u5ea6 37\u2103\uff08\u5e38\u89c4 CO\u2082\u57f9\u517b\u73af\u5883\u5373\u53ef\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff1b\u907f\u514d\u5f3a\u5149\u76f4\u5c04\uff0c\u51cf\u5c11 eGFP \u5149\u6f02\u767d\uff0c\u4fdd\u969c\u53cc\u8367\u5149\u89c2\u6d4b\u6548\u679c\uff09\u3002<\/p>\n

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\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u524d\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff0c\u51bb\u5b58\u6db2\u4e2d\u4e0d\u989d\u5916\u6dfb\u52a0 puro\uff0c\u4fdd\u7559\u53cc\u8367\u5149\u7279\u6027\u4e0e\u808b\u819c\u8f6c\u79fb\u76f8\u5173\u80fd\u529b\uff1b\u53ef\u9002\u5f53\u63d0\u9ad8\u51bb\u5b58\u5bc6\u5ea6\uff0c\u51cf\u5c11\u590d\u82cf\u635f\u4f24\uff0c\u590d\u82cf\u540e\u4f18\u5148\u89c2\u5bdf\u53cc\u8367\u5149\u8868\u8fbe\u4e0e\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\uff09\u3002<\/p>\n

<\/div>\n

\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\u3002<\/p>\n

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\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09\uff0c\u987a\u4e30\u5feb\u9012\uff1b\u8fd0\u8f93\u8fc7\u7a0b\u9700\u5c3d\u91cf\u51cf\u5c11\u98a0\u7c38\uff0c\u964d\u4f4e\u7ec6\u80de\u8131\u843d\u6982\u7387\u3002<\/p>\n

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\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\u3002<\/p><\/div>\n

\u7279\u522b\u8bf4\u660e<\/h2>\n
\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff0c\u4e14\u9700\u4e25\u683c\u4f7f\u7528\u4e13\u7528 McCoy’s 5A \u57f9\u517b\u57fa\u4ee5\u7ef4\u6301\u7ec6\u80de\u7279\u6027\u3002<\/div>\n","protected":false},"excerpt":{"rendered":"

\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1acalu-1-FLUC-eGFP-puro <\/p>\n

\n \u7ee7\u7eed\u9605\u8bfb <\/span><\/i><\/a>\n <\/div>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[18],"product_tag":[],"class_list":{"0":"post-1339","1":"product","2":"type-product","3":"status-publish","5":"product_cat-18","7":"first","8":"instock","9":"sale","10":"taxable","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product\/1339","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/comments?post=1339"}],"wp:attachment":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/media?parent=1339"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=1339"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=1339"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=1339"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}