{"id":1371,"date":"2025-10-04T03:09:08","date_gmt":"2025-10-03T19:09:08","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=1371"},"modified":"2025-10-04T03:09:09","modified_gmt":"2025-10-03T19:09:09","slug":"mda-mb-436-fluc-puro%e4%ba%ba%e4%b9%b3%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9efluc%e6%a0%87%e8%ae%b0","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/mda-mb-436-fluc-puro%e4%ba%ba%e4%b9%b3%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9efluc%e6%a0%87%e8%ae%b0\/","title":{"rendered":"MDA-MB-436-FLUC-puro\u4eba\u4e73\u817a\u764c\u7ec6\u80de(FLUC\u6807\u8bb0)"},"content":{"rendered":"<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u540d\u79f0\uff1aMDA-MB-436-FLUC-puro \u4eba\u4e73\u817a\u764c\u7ec6\u80de 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puro\uff08\u560c\u5464\u9709\u7d20\uff09\u836f\u7b5b\u6d53\u5ea6\u4e3a 1.0\u03bcg\/ml\uff0c\u7528\u4e8e\u53bb\u9664\u672a\u8f6c\u67d3\u7ec6\u80de\uff1b\u57f9\u517b\u8fc7\u7a0b\u4e2d\u5efa\u8bae\u4f7f\u7528 0.5\u03bcg\/ml \u6d53\u5ea6 puro \u7ef4\u6301\u3002\u4f4e\u6d53\u5ea6\u7ef4\u6301\u53ef\u4fdd\u969c FLUC \u57fa\u56e0\u7a33\u5b9a\u8868\u8fbe\uff0c\u540c\u65f6\u4e0d\u5f71\u54cd\u7ec6\u80de\u8d34\u58c1\u751f\u957f\u3001\u591a\u89d2\u5f62\u5f62\u6001\u53ca\u8f6c\u79fb\u76f8\u5173\u7279\u6027\u3002<\/div>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u7ec6\u80de\u57f9\u517b\u6838\u5fc3\u4fe1\u606f<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57fa\u7840\u53c2\u6570<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff1b<\/li>\n<li>\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff0c\u907f\u514d\u5e72\u6270\u7ec6\u80de\u589e\u6b96\u3001FLUC \u6d3b\u6027\u53ca\u4e73\u817a\u764c\u8f6c\u79fb\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u6570\u636e\u51c6\u786e\u6027\uff09\uff1b<\/li>\n<li>\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff0c\u53ef\u901a\u8fc7\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u6216\u4f9b\u5e94\u5546\u83b7\u53d6\u8be6\u7ec6\u6eaf\u6e90\u4fe1\u606f\uff0c\u786e\u4fdd\u7ec6\u80de\u682a\u7a33\u5b9a\u6027\u4e0e\u80f8\u6c34\u8f6c\u79fb\u7279\u6027\u53ef\u9760\u3002<\/li>\n<\/ul>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u4e0e\u51bb\u5b58<\/h3>\n<h4 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u57f9\u517b\u57fa\u914d\u65b9\uff08\u5173\u952e\u6210\u5206\u4e0d\u53ef\u7701\u7565\uff09<\/h4>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">90% L15 \u57f9\u517b\u57fa + 15% FBS\uff08\u80ce\u725b\u8840\u6e05\uff09 + PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\u53cc\u6297\uff09 + 16\u03bcg\/ml \u8c37\u80f1\u7518\u80bd\uff08\u8fd8\u539f\u578b\uff09 + 0.01mg\/ml \u80f0\u5c9b\u7d20 + 0.5\u03bcg\/ml puro<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u6ce8\uff1a\u9700\u4f7f\u7528 L15 \u4e13\u7528\u57f9\u517b\u57fa\uff08\u53ef\u5728\u65e0 CO\u2082\u73af\u5883\u4e0b\u7ef4\u6301 pH \u7a33\u5b9a\uff09\uff0c\u4e0d\u53ef\u66ff\u6362\u4e3a DMEM\u3001RPMI-1640 \u7b49\u57f9\u517b\u57fa\uff1b\u8c37\u80f1\u7518\u80bd\u4e0e\u80f0\u5c9b\u7d20\u4e3a\u7ec6\u80de\u751f\u957f\u5fc5\u9700\u56e0\u5b50\uff0c\u9700\u7cbe\u51c6\u6dfb\u52a0\u3002<\/p><\/div>\n<h4 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u57f9\u517b\u6761\u4ef6<\/h4>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u6c14\u76f8\uff1a100% \u7a7a\u6c14\uff08\u4e25\u7981\u901a\u5165 CO\u2082\uff09\uff1b\u6e29\u5ea6\uff1a37\u2103\uff1b<\/li>\n<li>\u64cd\u4f5c\u6ce8\u610f\u4e8b\u9879\uff1a\u6362\u6db2\u3001\u4f20\u4ee3\u65f6\u52a8\u4f5c\u8f7b\u67d4\uff0c\u907f\u514d\u635f\u4f24\u591a\u89d2\u5f62\u7ec6\u80de\u5f62\u6001\uff1b\u4e25\u683c\u9075\u5faa \u201c\u6bcf\u65e5\u6362 1-2 \u6b21\u7a7a\u6c14\u201d \u7684\u8981\u6c42\uff08\u82e5\u7528\u5bc6\u5c01\u76d6\u57f9\u517b\u74f6\uff09\uff0c\u6bcf\u6b21\u6362\u6c14\u65f6\u95f4\u63a7\u5236\u5728 1-2 \u5206\u949f\uff0c\u51cf\u5c11\u73af\u5883\u6e29\u5ea6\u6ce2\u52a8\u5bf9\u7ec6\u80de\u7684\u5f71\u54cd\u3002<\/li>\n<\/ul>\n<h4 class=\"header-iWP5WJ auto-hide-last-sibling-br\">3. \u51bb\u5b58\u6761\u4ef6<\/h4>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u51bb\u5b58\u6db2\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff1b<\/li>\n<li>\u64cd\u4f5c\u8981\u70b9\uff1a\u51bb\u5b58\u524d\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff0c\u4fdd\u7559 FLUC \u6d3b\u6027\u4e0e\u8f6c\u79fb\u7279\u6027\uff1b\u6309 1\u00d710\u2076-2\u00d710\u2076cells\/ml \u5bc6\u5ea6\u5206\u88c5\uff0c\u68af\u5ea6\u964d\u6e29\u540e\u5b58\u5165\u6db2\u6c2e\uff1b\u590d\u82cf\u540e\u4f18\u5148\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\u3001\u591a\u89d2\u5f62\u5f62\u6001\u53ca\u751f\u957f\u72b6\u6001\uff0c\u4e14\u590d\u82cf\u540e\u4ecd\u9700\u9075\u5faa\u65e0 CO\u2082\u57f9\u517b\u8981\u6c42\u3002<\/li>\n<\/ul>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u8d27\u671f\u4e0e\u8fd0\u8f93<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff1b<\/li>\n<li>\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09\uff0c\u987a\u4e30\u5feb\u9012\uff1b\u8fd0\u8f93\u590d\u82cf\u7ec6\u80de\u65f6\uff0c\u9700\u4f7f\u7528\u5bc6\u5c01\u5305\u88c5\u907f\u514d 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