{"id":1380,"date":"2025-10-04T03:39:09","date_gmt":"2025-10-03T19:39:09","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=1380"},"modified":"2025-10-04T03:39:10","modified_gmt":"2025-10-03T19:39:10","slug":"cho-k1-gfp-puro%e4%b8%ad%e5%9b%bd%e4%bb%93%e9%bc%a0%e5%8d%b5%e5%b7%a2%e7%bb%86%e8%83%9egfp%e6%a0%87%e8%ae%b0","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/cho-k1-gfp-puro%e4%b8%ad%e5%9b%bd%e4%bb%93%e9%bc%a0%e5%8d%b5%e5%b7%a2%e7%bb%86%e8%83%9egfp%e6%a0%87%e8%ae%b0\/","title":{"rendered":"CHO-K1-GFP-puro\u4e2d\u56fd\u4ed3\u9f20\u5375\u5de2\u7ec6\u80de(GFP\u6807\u8bb0)"},"content":{"rendered":"<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u540d\u79f0\uff1aCHO-K1-GFP-puro \u4e2d\u56fd\u4ed3\u9f20\u5375\u5de2\u7ec6\u80de (GFP \u6807\u8bb0)<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u79cd\u5c5e\u6765\u6e90\uff1a\u4ed3\u9f20<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec4\u7ec7\u6765\u6e90\uff1a\u5375\u5de2<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u5f62\u6001\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u6027\u522b\uff1a\u96cc<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u4ee3\u6570\uff1a10 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\u7ef4\u6301\uff0c\u9700\u6ce8\u610f\u5176\u836f\u7b5b\u6d53\u5ea6\u9ad8\u4e8e\u5e38\u89c4\u4eba\u7c7b\u7ec6\u80de\uff0c\u907f\u514d\u6d53\u5ea6\u6df7\u6dc6<\/p><\/div>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u80cc\u666f\u4e0e\u7b5b\u9009\u9a8c\u8bc1<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u80cc\u666f\u4ecb\u7ecd<\/h3>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">CHO-K1-GFP-puro \u7ec6\u80de\u7a33\u5b9a\u8868\u8fbe\u7eff\u8272\u8367\u5149\u86cb\u767d\uff08GFP\uff09\uff0c\u6027\u72b6\u7a33\u5b9a\uff0c\u53ef\u7528\u4f5c GFP \u8367\u5149\u68c0\u6d4b\u7684\u9633\u6027\u5bf9\u7167\uff0c\u4e5f\u9002\u7528\u4e8e\u86cb\u767d\u8868\u8fbe\u3001\u836f\u7269\u7b5b\u9009\u7b49\u5b9e\u9a8c\uff08\u5982\u91cd\u7ec4\u86cb\u767d\u751f\u4ea7\u3001\u836f\u7269\u6bd2\u6027\u8bc4\u4f30\uff09\u3002<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u8be5\u7ec6\u80de\u4fdd\u7559 CHO-K1 \u7ec6\u80de\u7684\u6838\u5fc3\u7279\u6027 \u2014\u2014 \u6613\u4e8e\u8f6c\u67d3\u3001\u53ef\u9ad8\u6548\u8868\u8fbe\u5916\u6e90\u86cb\u767d\uff0c\u662f\u751f\u7269\u5236\u836f\u9886\u57df\u91cd\u7ec4\u86cb\u767d\u751f\u4ea7\u3001\u5206\u5b50\u751f\u7269\u5b66\u5b9e\u9a8c\u7684\u5e38\u7528\u5de5\u5177\u7ec6\u80de\u682a\u3002<\/p><\/div>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">puro \u836f\u7b5b\u6d53\u5ea6\u8bf4\u660e<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u7b5b\u9009\u9636\u6bb5\uff1apuro \u6d53\u5ea6\u4e3a 4.0\u03bcg\/ml\uff0c\u56e0\u4ed3\u9f20\u7ec6\u80de\u5bf9 puro \u6297\u6027\u8f83\u9ad8\uff0c\u9700\u66f4\u9ad8\u6d53\u5ea6\u624d\u80fd\u6709\u6548\u6e05\u9664\u672a\u8f6c\u67d3\u7ec6\u80de\uff0c\u786e\u4fdd GFP \u9633\u6027\u7ec6\u80de\u7eaf\u5ea6\uff1b<\/li>\n<li>\u7ef4\u6301\u9636\u6bb5\uff1apuro \u6d53\u5ea6\u964d\u81f3 1.0\u03bcg\/ml\uff0c\u65e2\u80fd\u7a33\u5b9a\u7ec6\u80de\u7684\u836f\u7269\u6297\u6027\u4e0e GFP \u8868\u8fbe\uff0c\u53c8\u53ef\u907f\u514d\u9ad8\u6d53\u5ea6\u836f\u7269\u5bf9\u7ec6\u80de\u8d34\u58c1\u80fd\u529b\u3001\u86cb\u767d\u8868\u8fbe\u529f\u80fd\u7684\u6291\u5236\u3002<\/li>\n<\/ul>\n<hr class=\"auto-hide-last-sibling-br\" \/>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u7ec6\u80de\u57f9\u517b\u6838\u5fc3\u4fe1\u606f<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57fa\u7840\u53c2\u6570<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff1b<\/li>\n<li>\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff0c\u907f\u514d\u5e72\u6270\u7ec6\u80de\u589e\u6b96\u3001GFP \u8367\u5149\u8868\u8fbe\u53ca\u86cb\u767d\u8868\u8fbe \/ \u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u6570\u636e\u51c6\u786e\u6027\uff09\uff1b<\/li>\n<li>\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff0c\u53ef\u901a\u8fc7\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u6216\u4f9b\u5e94\u5546\u83b7\u53d6\u8be6\u7ec6\u6eaf\u6e90\u4fe1\u606f\uff0c\u786e\u4fdd\u7ec6\u80de\u682a\u7a33\u5b9a\u6027\u4e0e CHO-K1 \u7ec6\u80de\u5c5e\u6027\u53ef\u9760\u3002<\/li>\n<\/ul>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u4e0e\u51bb\u5b58<\/h3>\n<h4 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u57f9\u517b\u57fa\u914d\u65b9\uff08\u5173\u952e\u6210\u5206\u4e0d\u53ef\u7701\u7565\uff09<\/h4>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">DMEM \u57f9\u517b\u57fa + 10% FBS\uff08\u80ce\u725b\u8840\u6e05\uff09+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\u53cc\u6297\uff09+10\u03bcg\/ml \u812f\u6c28\u9178 + 1.0\u03bcg\/ml puro\uff08\u5e38\u89c4\u7ef4\u6301\u57f9\u517b\uff09\uff1b<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u6ce8\uff1a\u812f\u6c28\u9178\u4e3a CHO-K1 \u7ec6\u80de\u751f\u957f\u5fc5\u9700\u6c28\u57fa\u9178\uff08\u8be5\u7ec6\u80de\u81ea\u8eab\u65e0\u6cd5\u5408\u6210\uff09\uff0c\u4e0d\u53ef\u7701\u7565\uff1b\u836f\u7b5b\u65f6\u9700\u5c06 puro \u6d53\u5ea6\u8c03\u6574\u4e3a 4.0\u03bcg\/ml\u3002<\/p><\/div>\n<h4 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u57f9\u517b\u6761\u4ef6<\/h4>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u5e38\u89c4 CO\u2082\u57f9\u517b\u73af\u5883\u5373\u53ef\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff09\uff1b<\/li>\n<li>\u64cd\u4f5c\u6ce8\u610f\u4e8b\u9879\uff1a\u907f\u514d\u5f3a\u5149\u76f4\u5c04\u57f9\u517b\u74f6\uff0c\u51cf\u5c11 GFP \u5149\u6f02\u767d\uff0c\u4fdd\u969c\u8367\u5149\u89c2\u6d4b\u6548\u679c\uff1b\u64cd\u4f5c\u65f6\u52a8\u4f5c\u8f7b\u67d4\uff0c\u907f\u514d\u9891\u7e41\u79fb\u52a8\u5f71\u54cd\u7ec6\u80de\u8d34\u58c1\uff1b\u5f85\u7ec6\u80de\u5bc6\u5ea6\u8fbe 80%-90% \u65f6\u53ca\u65f6\u4f20\u4ee3\uff0cCHO-K1 \u7ec6\u80de\u589e\u6b96\u8f83\u5feb\uff0c\u8fc7\u5ea6\u5bc6\u96c6\u6613\u5bfc\u81f4\u8425\u517b\u8017\u5c3d\u3002<\/li>\n<\/ul>\n<h4 class=\"header-iWP5WJ auto-hide-last-sibling-br\">3. \u51bb\u5b58\u6761\u4ef6<\/h4>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u51bb\u5b58\u6db2\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff1b<\/li>\n<li>\u64cd\u4f5c\u8981\u70b9\uff1a\u51bb\u5b58\u524d\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff0c\u4fdd\u7559 GFP \u8367\u5149\u6d3b\u6027\u4e0e\u86cb\u767d\u8868\u8fbe\u80fd\u529b\uff1b\u6309 1\u00d710\u2076-2\u00d710\u2076cells\/ml \u5bc6\u5ea6\u5206\u88c5\uff0c\u68af\u5ea6\u964d\u6e29\u540e\u5b58\u5165\u6db2\u6c2e\uff1b\u590d\u82cf\u540e\u4f18\u5148\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\u3001\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\u53ca GFP \u8367\u5149\u5f3a\u5ea6\uff0c\u786e\u8ba4\u7ec6\u80de\u529f\u80fd\u6b63\u5e38\u3002<\/li>\n<\/ul>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u8d27\u671f\u4e0e\u8fd0\u8f93<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff1b<\/li>\n<li>\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ 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href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/cho-k1-gfp-puro%e4%b8%ad%e5%9b%bd%e4%bb%93%e9%bc%a0%e5%8d%b5%e5%b7%a2%e7%bb%86%e8%83%9egfp%e6%a0%87%e8%ae%b0\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        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