{"id":142,"date":"2025-08-23T15:43:45","date_gmt":"2025-08-23T07:43:45","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=142"},"modified":"2025-08-23T15:43:45","modified_gmt":"2025-08-23T07:43:45","slug":"293ft%e4%ba%ba%e8%83%9a%e8%82%be%e7%bb%86%e8%83%9e","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/293ft%e4%ba%ba%e8%83%9a%e8%82%be%e7%bb%86%e8%83%9e\/","title":{"rendered":"293FT\u4eba\u80da\u80be\u7ec6\u80de"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u540d\u79f0\uff1a293FT \u4eba\u80da\u80be\u7ec6\u80de<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u522b\u79f0\uff1aHEK-293FT; HEK 293FT; HEK-293-FT; HEK293FT; 293-FT; 293FT; 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data-list=\"bullet1\">\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f\uff08\u6838\u5fc3\u7279\u6027\uff1a\u8d34\u58c1\u4e0d\u7262\uff0c\u76f8\u8f83\u4e8e HEK-293A \u8d34\u58c1\u80fd\u529b\u663e\u8457\u8f83\u5f31\uff0c\u57f9\u517b\u53ca\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u6613\u51fa\u73b0\u7ec6\u80de\u8131\u843d\uff0c\u9700\u7279\u6b8a\u64cd\u4f5c\u7ef4\u62a4\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u5f62\u6001\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u7ec6\u80de\u5448\u591a\u8fb9\u5f62\uff0c\u6392\u5217\u677e\u6563\uff0c\u80de\u8d28\u900f\u4eae\uff0c\u6838\u4ec1\u660e\u663e\uff0c\u56e0\u8d34\u58c1\u4e0d\u7262\uff0c\u955c\u4e0b\u5e38\u89c2\u5bdf\u5230\u5c11\u91cf\u60ac\u6d6e\u7ec6\u80de\uff0c\u9700\u7ed3\u5408\u79bb\u5fc3\u6536\u96c6\u64cd\u4f5c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u6ce8\u610f\uff1a293FT 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data-list=\"bullet1\">\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4ee3\u6570\u7ec6\u80de\u80fd\u66f4\u597d\u4fdd\u7559 SV40 \u5927 T \u6297\u539f\u8868\u8fbe\u53ca\u75c5\u6bd2\u5305\u88c5\u80fd\u529b\uff0c\u5efa\u8bae\u4f20\u4ee3\u6b21\u6570\u4e0d\u8d85\u8fc7 12 \u4ee3\uff0c\u9632\u6b62\u957f\u671f\u4f20\u4ee3\u5bfc\u81f4\u7279\u6027\u6f02\u79fb\uff0c\u5982\u8d34\u58c1\u80fd\u529b\u8fdb\u4e00\u6b65\u4e0b\u964d\u3001\u6297\u539f\u8868\u8fbe\u51cf\u5f31\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u80cc\u666f\u7b80\u4ecb\uff1a293FT \u7ec6\u80de\u662f HEK-293 \u7ec6\u80de\u7684\u91cd\u8981\u884d\u751f\u682a\uff0c\u6838\u5fc3\u7279\u6027\u53ca\u5e94\u7528\u5982\u4e0b\uff1a\u2460\u5173\u952e\u86cb\u767d\u8868\u8fbe\uff1a\u7a33\u5b9a\u8868\u8fbe SV40 \u5927 T \u6297\u539f\uff0c\u8be5\u6297\u539f\u53ef\u4e0e\u7ec6\u80de\u5185 p53\u3001Rb 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\u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u51bb\u5b58\u7ba1\u5efa\u8bae\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u5b58\u6d3b\u7387\u226580%\uff08\u56e0\u8d34\u58c1\u4e0d\u7262\uff0c\u5b58\u6d3b\u7387\u7565\u4f4e\u4e8e HEK-293A\uff09\uff0c\u4e14\u590d\u82cf\u540e SV40 \u5927 T \u6297\u539f\u8868\u8fbe\u7a33\u5b9a\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u5747\u901a\u8fc7 PCR 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data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a90% DMEM+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff0c\u914d\u65b9\u4f18\u5316\u5efa\u8bae\uff1a\u2460DMEM \u9009\u62e9\u9ad8\u7cd6\u578b\uff08\u542b 4.5g\/L \u8461\u8404\u7cd6\uff09\uff0c\u6ee1\u8db3\u7ec6\u80de\u5feb\u901f\u589e\u6b96\u53ca\u75c5\u6bd2\u5305\u88c5\u7684\u9ad8\u80fd\u91cf\u9700\u6c42\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u6742\u8d28\u5f71\u54cd\u75c5\u6bd2\u4ea7\u7269\u6d3b\u6027\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u53ca\u75c5\u6bd2\u6d3b\u6027\u3002\u57f9\u517b\u57fa\u9700 37\u2103\u9884\u70ed 15-20 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data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u7279\u6b8a\u6ce8\u610f\uff1a\u2460\u56e0\u8d34\u58c1\u4e0d\u7262\uff0c\u6d88\u5316\u65f6\u4ec5\u9700 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 1 \u5206\u949f\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u5f00\u59cb\u8131\u843d\u5373\u53ef\u7ec8\u6b62\uff1b\u2461\u79bb\u5fc3\u6536\u96c6\u540e\u7528\u9884\u51b7\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e\u5e38\u89c4\u7ec6\u80de\uff09\uff0c\u63d0\u5347\u590d\u82cf\u540e\u7ec6\u80de\u5bc6\u5ea6\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\u540c HEK-293A\uff0c\u590d\u82cf\u65f6\u9700\u5feb\u901f\u878d\u5316\u5e76\u7acb\u5373\u79bb\u5fc3\uff0c\u907f\u514d\u60ac\u6d6e\u7ec6\u80de\u957f\u65f6\u95f4\u66b4\u9732\u5728 DMSO \u4e2d\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 SV40 \u5927 T \u6297\u539f\u8868\u8fbe\u68c0\u6d4b\u53ca\u75c5\u6bd2\u5305\u88c5\u80fd\u529b\u9a8c\u8bc1\uff0c\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u7279\u6b8a\u9700\u6c42\u5982\u52a0\u6025\u63d0\u4f9b\u6297\u539f\u8868\u8fbe\u9a8c\u8bc1\u62a5\u544a\uff0c\u53ef\u63d0\u524d\u6c9f\u901a\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u8fd0\u8f93\u65f6\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\uff0c\u51cf\u5c11\u8fd0\u8f93\u98a0\u7c38\u5bfc\u81f4\u7684\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u4f18\u5148\u5904\u7406\uff0c\u6309 \u201c\u7279\u522b\u6ce8\u610f\u201d 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data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff1b\u82e5\u7528\u4e8e\u75c5\u6bd2\u8f7d\u4f53\u751f\u4ea7\uff0c\u9700\u7b26\u5408\u300a\u57fa\u56e0\u5de5\u7a0b\u5b89\u5168\u7ba1\u7406\u529e\u6cd5\u300b\uff0c\u786e\u4fdd\u75c5\u6bd2\u4ea7\u7269\u4ec5\u7528\u4e8e\u79d1\u7814\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" 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