{"id":1430,"date":"2025-10-05T23:05:19","date_gmt":"2025-10-05T15:05:19","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&p=1430"},"modified":"2025-10-05T23:05:20","modified_gmt":"2025-10-05T15:05:20","slug":"skov3-gfp-fluc-puro%e4%ba%ba%e5%8d%b5%e5%b7%a2%e7%99%8c%e7%bb%86%e8%83%9egfp-fluc%e6%a0%87%e8%ae%b0","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/skov3-gfp-fluc-puro%e4%ba%ba%e5%8d%b5%e5%b7%a2%e7%99%8c%e7%bb%86%e8%83%9egfp-fluc%e6%a0%87%e8%ae%b0\/","title":{"rendered":"SKOV3-GFP-FLUC-puro\u4eba\u5375\u5de2\u764c\u7ec6\u80de(GFP\/FLUC\u6807\u8bb0)"},"content":{"rendered":"

\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n
\u7ec6\u80de\u540d\u79f0\uff1aSKOV3-GFP-FLUC-puro \u4eba\u5375\u5de2\u764c\u7ec6\u80de (GFP\/FLUC \u53cc\u6807\u8bb0)<\/p>\n
<\/div>\n

\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/p>\n

<\/div>\n

\u7ec4\u7ec7\u6765\u6e90\uff1a\u5375\u5de2<\/p>\n

<\/div>\n

\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f<\/p>\n

<\/div>\n

\u7ec6\u80de\u5f62\u6001\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837<\/p>\n

<\/div>\n

puro \u836f\u7b5b\u6d53\u5ea6\uff1a\u8be5\u7ec6\u80de puro\uff08\u560c\u5464\u9709\u7d20\uff09\u836f\u7b5b\u6d53\u5ea6\u4e3a 2.0\u03bcg\/ml\uff0c\u7528\u4e8e\u53bb\u9664\u672a\u8f6c\u67d3\u7ec6\u80de\uff1b\u57f9\u517b\u8fc7\u7a0b\u4e2d\u5efa\u8bae\u4f7f\u7528 1.0\u03bcg\/ml puro \u7ef4\u6301\uff0c\u4fdd\u969c GFP \u4e0e FLUC \u57fa\u56e0\u7a33\u5b9a\u8868\u8fbe<\/p>\n

<\/div>\n

\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4f20\u4ee3\uff0c\u66f4\u597d\u7ef4\u6301\u53cc\u6807\u8bb0\u6d3b\u6027\u53ca\u5375\u5de2\u764c\u7ec6\u80de\u751f\u7269\u5b66\u7279\u6027\uff09<\/p>\n

<\/div>\n

\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5<\/p>\n

<\/div>\n

\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff0c\u907f\u514d\u5e72\u6270\u7ec6\u80de\u589e\u6b96\u3001\u53cc\u6807\u8bb0\u6d3b\u6027\u53ca\u5375\u5de2\u764c\u76f8\u5173\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u6570\u636e\u51c6\u786e\u6027\uff09<\/p><\/div>\n

\u80cc\u666f\u4e0e\u7b5b\u9009\u9a8c\u8bc1<\/h1>\n

\u80cc\u666f\u4ecb\u7ecd<\/h2>\n
SKOV3-GFP-FLUC-puro \u7ec6\u80de\u7a33\u5b9a\u8868\u8fbe\u7eff\u8272\u8367\u5149\u86cb\u767d\uff08GFP\uff09\u4e0e\u8367\u5149\u7d20\u9176\uff08FLUC\uff09\u53cc\u6807\u8bb0\uff0cGFP \u53ef\u76f4\u89c2\u89c2\u5bdf\u7ec6\u80de\u5f62\u6001\u4e0e\u5206\u5e03\uff0cFLUC \u53ef\u901a\u8fc7\u6d3b\u4f53\u6210\u50cf\u91cf\u5316\u8ffd\u8e2a\uff0c\u6027\u72b6\u7a33\u5b9a\u3002\u8be5\u7ec6\u80de\u4fdd\u7559 SKOV3 \u7ec6\u80de\u6838\u5fc3\u7279\u6027\uff08\u5178\u578b\u4eba\u5375\u5de2\u764c\u7ec6\u80de\u7279\u5f81\uff09\uff0c\u9002\u7528\u4e8e\u5375\u5de2\u764c\u4f53\u5185\u5916\u591a\u7ef4\u5ea6\u5b9e\u9a8c\uff0c\u662f\u7814\u7a76\u5375\u5de2\u764c\u53d1\u75c5\u673a\u5236\u3001\u6297\u80bf\u7624\u836f\u7269\u7b5b\u9009\uff08\u5982\u6297\u8179\u8154\u8f6c\u79fb\u3001\u9776\u5411\u6cbb\u7597\u6548\u679c\u8bc4\u4f30\uff09\u7684\u4e13\u7528\u7ec6\u80de\u682a\u3002<\/div>\n

\u7ec6\u80de\u57f9\u517b\u6838\u5fc3\u4fe1\u606f<\/h1>\n

\u57fa\u7840\u53c2\u6570<\/h2>\n
\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff0c\u53ef\u901a\u8fc7\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u6216\u4f9b\u5e94\u5546\u83b7\u53d6\u8be6\u7ec6\u6eaf\u6e90\u4fe1\u606f\uff0c\u786e\u4fdd\u7ec6\u80de\u682a\u7a33\u5b9a\u6027\u4e0e\u4eba\u5375\u5de2\u764c\u7ec6\u80de\u5c5e\u6027\u53ef\u9760\u3002<\/div>\n

\u57f9\u517b\u4e0e\u51bb\u5b58<\/h2>\n
\u57f9\u517b\u57fa\u914d\u65b9\uff1a90% McCoy\u2019s 5A \u57f9\u517b\u57fa + 10% FBS\uff08\u80ce\u725b\u8840\u6e05\uff09 + \u53cc\u6297\uff08\u9752\u9709\u7d20 – \u94fe\u9709\u7d20\uff09\uff1b\u836f\u7b5b\u6216\u5e38\u89c4\u7ef4\u6301\u65f6\uff0c\u9700\u989d\u5916\u6dfb\u52a0\u5bf9\u5e94\u6d53\u5ea6 puro\uff08\u836f\u7b5b 2.0\u03bcg\/ml\uff0c\u7ef4\u6301 1.0\u03bcg\/ml\uff09\uff0c\u9002\u914d\u8d34\u58c1\u751f\u957f\u7684\u5375\u5de2\u764c\u7ec6\u80de\u8425\u517b\u9700\u6c42\uff0c\u65e0\u9700\u989d\u5916\u6dfb\u52a0\u7279\u6b8a\u56e0\u5b50\u3002<\/div>\n
<\/div>\n
\u57f9\u517b\u6761\u4ef6\uff1a<\/p>\n
<\/div>\n

\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u5e38\u89c4 CO\u2082\u57f9\u517b\u73af\u5883\u5373\u53ef\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff0c\u9700\u786e\u4fdd\u57f9\u517b\u7bb1\u6e29\u5ea6\u3001\u6e7f\u5ea6\u7a33\u5b9a\uff09\uff1b<\/p><\/div>\n

<\/div>\n
\u64cd\u4f5c\u6ce8\u610f\u4e8b\u9879\uff1a<\/div>\n
<\/div>\n
    \n
  1. \u4f20\u4ee3\u65f6\u7528\u80f0\u9176\u6e29\u548c\u6d88\u5316\u8d34\u58c1\u7ec6\u80de\uff0c\u5f85\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\u8fb9\u7f18\u6536\u7f29\u3001\u95f4\u9699\u589e\u5927\u540e\uff0c\u7acb\u5373\u52a0\u542b 1.0\u03bcg\/ml puro \u7684\u57f9\u517b\u57fa\u7ec8\u6b62\u6d88\u5316\uff0c\u907f\u514d\u8fc7\u5ea6\u6d88\u5316\u635f\u4f24\u7ec6\u80de\uff1b<\/li>\n
  2. \u5b9a\u671f\u89c2\u5bdf\u7ec6\u80de\u5bc6\u5ea6\uff0c\u5f85\u5bc6\u5ea6\u8fbe 70%-80% \u65f6\u53ca\u65f6\u4f20\u4ee3\uff0c\u9632\u6b62\u8fc7\u5ea6\u5bc6\u96c6\u5bfc\u81f4 GFP\/FLUC \u6d3b\u6027\u4e0b\u964d\u6216\u7ec6\u80de\u51cb\u4ea1\uff1b<\/li>\n
  3. GFP \u5bf9\u5149\u654f\u611f\uff0c\u9700\u907f\u514d\u957f\u65f6\u95f4\u5f3a\u5149\u76f4\u5c04\u57f9\u517b\u74f6\uff0c\u51cf\u5c11\u8367\u5149\u4fe1\u53f7\u6dec\u706d\uff1bFLUC \u5bf9\u5149\u7a33\u5b9a\u6027\u8f83\u597d\uff0c\u4f46\u4ecd\u5efa\u8bae\u540c\u6b65\u505a\u597d\u907f\u5149\u9632\u62a4\uff1b<\/li>\n
  4. \u6362\u6db2\u65f6\u9700\u4f7f\u7528\u65b0\u9c9c\u914d\u7f6e\u7684\u542b 1.0\u03bcg\/ml puro \u7684\u57f9\u517b\u57fa\uff0c\u786e\u4fdd puro \u6d53\u5ea6\u7a33\u5b9a\uff0c\u7ef4\u6301\u7ec6\u80de\u6297\u6027\u4e0e\u53cc\u6807\u8bb0\u8868\u8fbe\u3002<\/li>\n<\/ol>\n
    <\/div>\n
    \u51bb\u5b58\u6761\u4ef6\uff1a<\/p>\n
    <\/div>\n

    \u51bb\u5b58\u6db2\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff1b<\/p><\/div>\n

    <\/div>\n
    \u64cd\u4f5c\u8981\u70b9\uff1a\u51bb\u5b58\u524d\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff0c\u4fdd\u7559 GFP\/FLUC \u6d3b\u6027\u4e0e\u5375\u5de2\u764c\u7ec6\u80de\u7279\u6027\uff1b\u80f0\u9176\u6d88\u5316\u6536\u96c6\u7ec6\u80de\u540e\uff0c\u7528\u542b 1.0\u03bcg\/ml puro \u7684\u57f9\u517b\u57fa\u6e05\u6d17 1 \u6b21\uff0c\u518d\u7528\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u7ec6\u80de\u5bc6\u5ea6\u81f3 1\u00d710\u2076-2\u00d710\u2076cells\/ml\uff1b\u68af\u5ea6\u964d\u6e29\uff084\u2103 30 \u5206\u949f\u2192-20\u2103 1 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u957f\u671f\u4fdd\u5b58\uff09\uff1b\u590d\u82cf\u65f6\u5feb\u901f\u5c06\u51bb\u5b58\u7ba1\u653e\u5165 37\u2103\u6c34\u6d74\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u542b 1.0\u03bcg\/ml puro \u7684\u65b0\u9c9c\u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u907f\u514d\u51bb\u5b58\u6db2\u6b8b\u7559\u5bf9\u7ec6\u80de\u7684\u6bd2\u6027\u5f71\u54cd\uff0c\u590d\u82cf\u540e\u4f18\u5148\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\u3001\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\u53ca GFP \u8367\u5149\u5f3a\u5ea6\uff0c\u540e\u7eed\u53ef\u901a\u8fc7 Luciferase \u68c0\u6d4b\u786e\u8ba4 FLUC \u6d3b\u6027\u3002<\/div>\n

    \u8d27\u671f\u4e0e\u8fd0\u8f93<\/h1>\n
    \u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff1b<\/p>\n
    <\/div>\n

    \u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09\uff0c\u987a\u4e30\u5feb\u9012\uff1b<\/p>\n

    <\/div>\n

    \u8fd0\u8f93\u590d\u82cf\u7ec6\u80de\u65f6\uff0c\u9700\u4f7f\u7528\u9632\u9707\u5305\u88c5\u51cf\u5c11\u98a0\u7c38\uff0c\u964d\u4f4e\u8d34\u58c1\u7ec6\u80de\u8131\u843d\u6982\u7387\uff1b\u540c\u65f6\u505a\u597d\u907f\u5149\u9632\u62a4\uff0c\u907f\u514d\u8fd0\u8f93\u8fc7\u7a0b\u4e2d GFP \u4fe1\u53f7\u635f\u8017\uff0c\u4fdd\u969c\u63a5\u6536\u540e\u7ec6\u80de\u6d3b\u6027\u4e0e\u53cc\u6807\u8bb0\u7a33\u5b9a\u6027\u3002<\/p><\/div>\n

    \u4f9b\u5e94\u9650\u5236<\/h1>\n
    \u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\u3002<\/div>\n

    \u7279\u522b\u8bf4\u660e<\/h1>\n
    \u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u5f00\u5c55\u5375\u5de2\u764c\u76f8\u5173\u5b9e\u9a8c\u65f6\uff0c\u53ef\u7ed3\u5408\u5176\u6613\u8179\u8154\u8f6c\u79fb\u7684\u7279\u6027\u8bbe\u8ba1\u8f6c\u79fb\u6a21\u578b\uff0c\u5229\u7528 GFP\uff08\u4f53\u5916\u89c2\u5bdf\uff09\u4e0e FLUC\uff08\u4f53\u5185\u8ffd\u8e2a\uff09\u53cc\u6807\u8bb0\u4f18\u52bf\uff0c\u591a\u7ef4\u5ea6\u76d1\u6d4b\u80bf\u7624\u751f\u957f\u4e0e\u8f6c\u79fb\u8fc7\u7a0b\uff0c\u7cbe\u51c6\u8bc4\u4f30\u836f\u7269\u5e72\u9884\u6548\u679c\uff0c\u63d0\u5347\u5b9e\u9a8c\u6570\u636e\u7684\u5168\u9762\u6027\u4e0e\u4e34\u5e8a\u76f8\u5173\u6027\u3002<\/div>\n","protected":false},"excerpt":{"rendered":"

    \u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aSKOV3-GFP-FLUC-puro \u4eba\u5375<\/p>\n

    \n \u7ee7\u7eed\u9605\u8bfb <\/span><\/i><\/a>\n <\/div>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[18],"product_tag":[],"class_list":{"0":"post-1430","1":"product","2":"type-product","3":"status-publish","5":"product_cat-18","7":"first","8":"instock","9":"sale","10":"taxable","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product\/1430","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/comments?post=1430"}],"wp:attachment":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/media?parent=1430"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=1430"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=1430"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=1430"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}