{"id":1432,"date":"2025-10-05T23:15:07","date_gmt":"2025-10-05T15:15:07","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=1432"},"modified":"2025-10-05T23:15:08","modified_gmt":"2025-10-05T15:15:08","slug":"mc38-ova%ef%bc%88%e5%b0%8f%e9%bc%a0%e7%bb%93%e8%82%a0%e7%99%8c%e7%bb%86%e8%83%9e-ova%e8%bd%ac%e6%9f%93%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/mc38-ova%ef%bc%88%e5%b0%8f%e9%bc%a0%e7%bb%93%e8%82%a0%e7%99%8c%e7%bb%86%e8%83%9e-ova%e8%bd%ac%e6%9f%93%ef%bc%89\/","title":{"rendered":"MC38-OVA\uff08\u5c0f\u9f20\u7ed3\u80a0\u764c\u7ec6\u80de-OVA\u8f6c\u67d3\uff09"},"content":{"rendered":"<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u540d\u79f0\uff1aMC38 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1.0\u03bcg\/ml\uff0c\u7528\u4e8e\u53bb\u9664\u672a\u8f6c\u67d3 \/ \u8fc7\u8868\u8fbe OVA \u7684\u7ec6\u80de\uff1b\u57f9\u517b\u8fc7\u7a0b\u4e2d\u5efa\u8bae\u4f7f\u7528 0.5\u03bcg\/ml puro \u7ef4\u6301\uff0c\u4fdd\u969c OVA \u7a33\u5b9a\u8fc7\u8868\u8fbe<\/p><\/div>\n<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u80cc\u666f\u4e0e\u7b5b\u9009\u9a8c\u8bc1<\/h1>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u80cc\u666f\u4ecb\u7ecd<\/h2>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">MC38 \u5c0f\u9f20\u7ed3\u80a0\u764c\u8fc7\u8868\u8fbe &#8211; OVA \u8f6c\u67d3\u7ec6\u80de\uff0c\u5728 MC38 \u5c0f\u9f20\u7ed3\u80a0\u764c\u7ec6\u80de\u57fa\u7840\u4e0a\u7a33\u5b9a\u8fc7\u8868\u8fbe\u5375\u6e05\u86cb\u767d\uff08OVA\uff09\u3002OVA 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br-paragraph-space\">\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff0c\u53ef\u901a\u8fc7\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u6216\u4f9b\u5e94\u5546\u83b7\u53d6\u8be6\u7ec6\u6eaf\u6e90\u4fe1\u606f\uff0c\u786e\u4fdd\u7ec6\u80de\u682a\u7a33\u5b9a\u6027\u3001OVA \u8fc7\u8868\u8fbe\u7279\u6027\u53ca\u5c0f\u9f20\u7ed3\u80a0\u764c\u7ec6\u80de\u5c5e\u6027\u53ef\u9760\u3002<\/div>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u4e0e\u51bb\u5b58<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u57fa\u914d\u65b9\uff08\u9700\u4e25\u683c\u914d\u7f6e\uff0c\u4fdd\u969c\u7ec6\u80de\u751f\u957f\u4e0e OVA \u8868\u8fbe\uff09<\/h3>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">DMEM \u57f9\u517b\u57fa + 10% FBS\uff08\u80ce\u725b\u8840\u6e05\uff09 + PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\u53cc\u6297\uff09 + 1% NEAA\uff08\u975e\u5fc5\u9700\u6c28\u57fa\u9178\uff09 + 1% \u4e19\u916e\u9178\u94a0 + 1% \u8c37\u6c28\u9170\u80fa + 1% HEPES\uff1b\u6240\u6709\u6dfb\u52a0\u6210\u5206\u5747\u4e3a\u7ec6\u80de\u751f\u957f\u5fc5\u9700\uff0c\u4e0d\u53ef\u7701\u7565\u6216\u66ff\u6362\uff1b\u9700\u836f\u7b5b\u6216\u7ef4\u6301 OVA \u8fc7\u8868\u8fbe\u65f6\uff0c\u989d\u5916\u6dfb\u52a0\u5bf9\u5e94\u6d53\u5ea6 puro\uff08\u836f\u7b5b 1.0\u03bcg\/ml\uff0c\u7ef4\u6301 0.5\u03bcg\/ml\uff09\u3002<\/div>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u6761\u4ef6<\/h3>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u5e38\u89c4 CO\u2082\u57f9\u517b\u73af\u5883\u5373\u53ef\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff0c\u9700\u786e\u4fdd\u57f9\u517b\u7bb1\u6e29\u5ea6\u3001\u6e7f\u5ea6\u7a33\u5b9a\uff0c\u907f\u514d\u73af\u5883\u6ce2\u52a8\u5f71\u54cd OVA \u8868\u8fbe\uff09\uff1b<\/div>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u64cd\u4f5c\u6ce8\u610f\u4e8b\u9879<\/h3>\n<ol class=\"auto-hide-last-sibling-br\">\n<li>\u4f20\u4ee3\u64cd\u4f5c\uff1a\u7528\u80f0\u9176\u6e29\u548c\u6d88\u5316\u8d34\u58c1\u7ec6\u80de\uff0c\u5f85\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\u8fb9\u7f18\u6536\u7f29\u3001\u95f4\u9699\u589e\u5927\u540e\uff0c\u7acb\u5373\u52a0\u542b 0.5\u03bcg\/ml puro \u7684\u57f9\u517b\u57fa\u7ec8\u6b62\u6d88\u5316\uff0c\u907f\u514d\u8fc7\u5ea6\u6d88\u5316\u635f\u4f24\u7ec6\u80de\uff1b<\/li>\n<li>\u5bc6\u5ea6\u63a7\u5236\uff1a\u5b9a\u671f\u89c2\u5bdf\u7ec6\u80de\u5bc6\u5ea6\uff0c\u5f85\u5bc6\u5ea6\u8fbe 70%-80% \u65f6\u53ca\u65f6\u4f20\u4ee3\uff0c\u9632\u6b62\u8fc7\u5ea6\u5bc6\u96c6\u5bfc\u81f4\u7ec6\u80de\u51cb\u4ea1\u6216 OVA \u8868\u8fbe\u91cf\u4e0b\u964d\uff1b<\/li>\n<li>\u57f9\u517b\u57fa\u7ba1\u7406\uff1a\u57f9\u517b\u57fa\u9700\u73b0\u914d\u73b0\u7528\uff0c\u6216\u914d\u7f6e\u540e 4\u2103\u77ed\u671f\u51b7\u85cf\uff08\u4e0d\u8d85\u8fc7 3 \u5929\uff09\uff0c\u907f\u514d NEAA\u3001\u8c37\u6c28\u9170\u80fa\u7b49\u6210\u5206\u6d3b\u6027\u964d\u4f4e\uff1b<\/li>\n<li>\u5b9e\u9a8c\u9002\u914d\uff1a\u7528\u4e8e\u514d\u75ab\u5b9e\u9a8c\u524d\uff0c\u5efa\u8bae\u901a\u8fc7 qPCR \u6216 WB \u518d\u6b21\u68c0\u6d4b OVA \u8868\u8fbe\u60c5\u51b5\uff0c\u786e\u4fdd\u6297\u539f\u8868\u8fbe\u7a33\u5b9a\uff0c\u63d0\u5347\u5b9e\u9a8c\u91cd\u590d\u6027\u3002<\/li>\n<\/ol>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u51bb\u5b58\u4e0e\u590d\u82cf<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u51bb\u5b58\u6db2\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff1b<\/li>\n<li>\u51bb\u5b58\u8981\u70b9\uff1a\u51bb\u5b58\u524d\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff08OVA \u8868\u8fbe\u7a33\u5b9a\uff09\uff0c\u80f0\u9176\u6d88\u5316\u6536\u96c6\u7ec6\u80de\u540e\uff0c\u7528\u542b 0.5\u03bcg\/ml puro \u7684\u57f9\u517b\u57fa\u6e05\u6d17 1 \u6b21\uff0c\u518d\u7528\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u7ec6\u80de\u5bc6\u5ea6\u81f3 1\u00d710\u2076-2\u00d710\u2076cells\/ml\uff1b\u68af\u5ea6\u964d\u6e29\uff084\u2103 30 \u5206\u949f\u2192-20\u2103 1 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u957f\u671f\u4fdd\u5b58\uff09\uff0c\u907f\u514d\u964d\u6e29\u8fc7\u5feb\u635f\u4f24\u7ec6\u80de\uff1b<\/li>\n<li>\u590d\u82cf\u8981\u70b9\uff1a\u4ece\u6db2\u6c2e\u53d6\u51fa\u51bb\u5b58\u7ba1\u540e\uff0c\u7acb\u5373\u653e\u5165 37\u2103\u6c34\u6d74\u878d\u5316\uff08\u7ea6 1-2 \u5206\u949f\uff09\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u5f03\u51bb\u5b58\u6db2\uff0c\u7528\u542b 0.5\u03bcg\/ml puro \u7684\u65b0\u9c9c\u57f9\u517b\u57fa\u91cd\u60ac\uff1b\u590d\u82cf\u540e\u4f18\u5148\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\u3001\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\uff0c\u540e\u7eed\u53ef\u901a\u8fc7 qPCR\/WB \u786e\u8ba4 OVA \u8fc7\u8868\u8fbe\u662f\u5426\u6b63\u5e38\u3002<\/li>\n<\/ul>\n<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u8d27\u671f\u4e0e\u53d1\u8d27<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff1b<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u53d1\u8d27\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09\uff0c\u987a\u4e30\u5feb\u9012\uff1b<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ 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class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u7279\u522b\u8bf4\u660e<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u5f00\u5c55\u80bf\u7624\u514d\u75ab\u5b9e\u9a8c\u65f6\uff0c\u53ef\u5229\u7528\u5176 OVA \u8fc7\u8868\u8fbe\u7279\u6027\uff0c\u6784\u5efa \u201c\u6297\u539f &#8211; \u514d\u75ab\u7ec6\u80de\u201d \u76f8\u4e92\u4f5c\u7528\u6a21\u578b\uff08\u5982 OVA \u7279\u5f02\u6027 T 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