{"id":1433,"date":"2025-10-05T23:17:25","date_gmt":"2025-10-05T15:17:25","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=1433"},"modified":"2025-10-05T23:17:25","modified_gmt":"2025-10-05T15:17:25","slug":"hepg2-ntcp-puro%e4%ba%ba%e8%82%9d%e7%99%8c%e7%bb%86%e8%83%9entcp%e7%a8%b3%e8%bd%ac","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/hepg2-ntcp-puro%e4%ba%ba%e8%82%9d%e7%99%8c%e7%bb%86%e8%83%9entcp%e7%a8%b3%e8%bd%ac\/","title":{"rendered":"HEPG2-NTCP-puro\u4eba\u809d\u764c\u7ec6\u80de(NTCP\u7a33\u8f6c)"},"content":{"rendered":"<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u540d\u79f0\uff1aHEPG2-NTCP-puro \u4eba\u809d\u764c\u7ec6\u80de (NTCP \u7a33\u8f6c\uff0cNTCP \u4e3a\u94a0 &#8211; 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class=\"auto-hide-last-sibling-br\">\n<li>\u4f20\u4ee3\u64cd\u4f5c\uff1a\u7528\u80f0\u9176\u6e29\u548c\u6d88\u5316\u8d34\u58c1\u7ec6\u80de\uff0c\u5f85\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\u8fb9\u7f18\u6536\u7f29\u3001\u95f4\u9699\u589e\u5927\u540e\uff0c\u7acb\u5373\u52a0\u542b 1.0\u03bcg\/ml puro \u7684\u57f9\u517b\u57fa\u7ec8\u6b62\u6d88\u5316\uff0c\u907f\u514d\u8fc7\u5ea6\u6d88\u5316\u635f\u4f24\u7ec6\u80de\uff1b<\/li>\n<li>\u5bc6\u5ea6\u63a7\u5236\uff1a\u5b9a\u671f\u89c2\u5bdf\u7ec6\u80de\u5bc6\u5ea6\uff0c\u5f85\u5bc6\u5ea6\u8fbe 70%-80% \u65f6\u53ca\u65f6\u4f20\u4ee3\uff0c\u9632\u6b62\u8fc7\u5ea6\u5bc6\u96c6\u5bfc\u81f4\u7ec6\u80de\u51cb\u4ea1\u6216 NTCP \u8868\u8fbe\u91cf\u4e0b\u964d\uff1b<\/li>\n<li>\u836f\u7269\u5b9e\u9a8c\u9002\u914d\uff1a\u7528\u4e8e\u6297\u4e59\u809d\u75c5\u6bd2\u836f\u7269\u7b5b\u9009\u65f6\uff0c\u5efa\u8bae\u5148\u901a\u8fc7 qPCR\/western blot \u786e\u8ba4 NTCP \u8868\u8fbe\u6b63\u5e38\uff0c\u518d\u8fdb\u884c\u75c5\u6bd2\u611f\u67d3\u64cd\u4f5c\uff0c\u63d0\u5347\u5b9e\u9a8c\u91cd\u590d\u6027\uff1b<\/li>\n<li>\u907f\u514d\u9891\u7e41\u6362\u6db2\uff1a\u6362\u6db2\u9891\u7387\u7ea6 2-3 \u5929 \/ \u6b21\uff0c\u6839\u636e\u57f9\u517b\u57fa\u989c\u8272\uff08\u53d8\u9ec4\u524d\uff09\u53ca\u65f6\u66f4\u6362\uff0c\u907f\u514d\u9891\u7e41\u64cd\u4f5c\u5e72\u6270\u7ec6\u80de\u751f\u957f\u4e0e NTCP \u8868\u8fbe\u3002<\/li>\n<\/ol>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u51bb\u5b58\u4e0e\u590d\u82cf<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u51bb\u5b58\u6db2\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff1b<\/li>\n<li>\u51bb\u5b58\u8981\u70b9\uff1a\u51bb\u5b58\u524d\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff08NTCP \u8868\u8fbe\u7a33\u5b9a\uff09\uff0c\u80f0\u9176\u6d88\u5316\u6536\u96c6\u7ec6\u80de\u540e\uff0c\u7528\u542b 1.0\u03bcg\/ml puro \u7684\u57f9\u517b\u57fa\u6e05\u6d17 1 \u6b21\uff0c\u518d\u7528\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u7ec6\u80de\u5bc6\u5ea6\u81f3 1\u00d710\u2076-2\u00d710\u2076cells\/ml\uff1b\u68af\u5ea6\u964d\u6e29\uff084\u2103 30 \u5206\u949f\u2192-20\u2103 1 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u957f\u671f\u4fdd\u5b58\uff09\uff0c\u907f\u514d\u964d\u6e29\u8fc7\u5feb\u635f\u4f24\u7ec6\u80de\uff1b<\/li>\n<li>\u590d\u82cf\u8981\u70b9\uff1a\u4ece\u6db2\u6c2e\u53d6\u51fa\u51bb\u5b58\u7ba1\u540e\uff0c\u7acb\u5373\u653e\u5165 37\u2103\u6c34\u6d74\u878d\u5316\uff08\u7ea6 1-2 \u5206\u949f\uff09\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u5f03\u51bb\u5b58\u6db2\uff0c\u7528\u542b 1.0\u03bcg\/ml puro \u7684\u65b0\u9c9c\u57f9\u517b\u57fa\u91cd\u60ac\uff1b\u590d\u82cf\u540e\u4f18\u5148\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\u3001\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\uff0c\u540e\u7eed\u53ef\u901a\u8fc7 qPCR\/western blot \u786e\u8ba4 NTCP 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