{"id":1439,"date":"2025-10-05T23:30:48","date_gmt":"2025-10-05T15:30:48","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=1439"},"modified":"2025-10-05T23:30:49","modified_gmt":"2025-10-05T15:30:49","slug":"%e5%b0%8f%e9%bc%a0%e9%aa%a8%e9%ab%93%e9%97%b4%e5%85%85%e8%b4%a8%e5%b9%b2%e7%bb%86%e8%83%9e%e6%b0%b8%e7%94%9f%e5%8c%96-gfp-purogfp%e6%a0%87%e8%ae%b0","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/%e5%b0%8f%e9%bc%a0%e9%aa%a8%e9%ab%93%e9%97%b4%e5%85%85%e8%b4%a8%e5%b9%b2%e7%bb%86%e8%83%9e%e6%b0%b8%e7%94%9f%e5%8c%96-gfp-purogfp%e6%a0%87%e8%ae%b0\/","title":{"rendered":"\u5c0f\u9f20\u9aa8\u9ad3\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u6c38\u751f\u5316-GFP-PURO(GFP\u6807\u8bb0)"},"content":{"rendered":"<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u540d\u79f0\uff1a\u5c0f\u9f20\u9aa8\u9ad3\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u6c38\u751f\u5316 &#8211; GFP-PURO (GFP \u6807\u8bb0\uff0c\u901a\u8fc7\u6c38\u751f\u5316\u5904\u7406\u7a81\u7834\u539f\u4ee3\u7ec6\u80de\u589e\u6b96\u9650\u5236\uff0cGFP \u53ef\u76f4\u89c2\u8ffd\u8e2a\u7ec6\u80de)<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u79cd\u5c5e\u6765\u6e90\uff1a\u5c0f\u9f20<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec4\u7ec7\u6765\u6e90\uff1a\u9aa8\u9ad3\u8840\u7ec4\u7ec7<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u5f62\u6001\uff1a\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\uff08\u5178\u578b\u9aa8\u9ad3\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u5f62\u6001\u7279\u5f81\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u80cc\u666f\u4ecb\u7ecd\uff1a\u9aa8\u9ad3\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\uff08BMSCs\uff09\u662f\u9aa8\u9ad3\u57fa\u8d28\u5e72\u7ec6\u80de\uff0c\u5bf9\u9020\u8840\u5e72\u7ec6\u80de\uff08HSC\uff09\u6709\u673a\u68b0\u652f\u6301\u4f5c\u7528\uff0c\u8fd8\u80fd\u5206\u6ccc IL-6\u3001IL-11\u3001LIF\u3001M-CSF\u3001SCF \u7b49\u751f\u957f\u56e0\u5b50\u652f\u6301\u9020\u8840\uff1b\u5177\u6709\u9aa8\u3001\u8f6f\u9aa8\u3001\u808c\u8089\u3001\u8102\u80aa\u7b49\u591a\u5411\u5206\u5316\u6f5c\u80fd\u3002\u539f\u4ee3 BMSCs \u542b\u91cf\u6781\u4f4e\uff08\u9aa8\u9ad3\u6709\u6838\u7ec6\u80de\u603b\u6570\u7684 0.001%~0.1%\uff09\uff0c\u4e14\u5206\u79bb\u96be\u5ea6\u5927\uff0c\u8be5\u7ec6\u80de\u901a\u8fc7\u6162\u75c5\u6bd2\u8f6c\u67d3\u643a\u5e26 SV40 \u57fa\u56e0\uff08\u5b9e\u73b0\u6c38\u751f\u5316\uff09\u4e0e GFP \u57fa\u56e0\uff08\u5b9e\u73b0\u8367\u5149\u6807\u8bb0\uff09\uff0c\u540c\u65f6\u643a\u5e26\u560c\u5464\u9709\u7d20\u6297\u6027\uff0c\u89e3\u51b3\u539f\u4ee3\u7ec6\u80de\u589e\u6b96\u4e0e\u8ffd\u8e2a\u96be\u9898<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4f20\u4ee3\uff0c\u66f4\u597d\u7ef4\u6301\u6c38\u751f\u5316\u7279\u6027\u3001GFP \u6d3b\u6027\u53ca\u9aa8\u9ad3\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u591a\u5411\u5206\u5316\u529f\u80fd\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff0c\u907f\u514d\u5e72\u6270\u7ec6\u80de\u589e\u6b96\u3001\u5206\u5316\u529f\u80fd\u53ca\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u6570\u636e\u51c6\u786e\u6027\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u57f9\u517b\u57fa\uff1aGFP-puro \u5c0f\u9f20\u9aa8\u9ad3\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u6c38\u751f\u5316\u4e13\u7528\u57f9\u517b\u57fa\uff08\u4e0d\u53ef\u7528\u666e\u901a\u57f9\u517b\u57fa\u66ff\u4ee3\uff0c\u4fdd\u969c\u7ec6\u80de\u6c38\u751f\u5316\u7279\u6027\u4e0e\u5206\u5316\u6f5c\u80fd\uff09<\/p><\/div>\n<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u80cc\u666f\u4e0e\u5e94\u7528\u4ef7\u503c<\/h1>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u6838\u5fc3\u4f18\u52bf<\/h2>\n<ol class=\"auto-hide-last-sibling-br\">\n<li>\u6c38\u751f\u5316\u7279\u6027\uff1a\u901a\u8fc7 SV40 \u57fa\u56e0\u8f6c\u67d3\uff0c\u7a81\u7834\u539f\u4ee3 BMSCs \u4f53\u5916\u589e\u6b96\u9650\u5236\uff0c\u53ef\u957f\u671f\u57f9\u517b\u4e14\u751f\u7269\u7279\u6027\u7a33\u5b9a\uff0c\u65e0\u9700\u9891\u7e41\u5206\u79bb\u539f\u4ee3\u7ec6\u80de\uff0c\u964d\u4f4e\u5b9e\u9a8c\u96be\u5ea6\uff1b<\/li>\n<li>\u53ef\u89c6\u5316\u8ffd\u8e2a\uff1a\u7a33\u5b9a\u8868\u8fbe GFP\uff0c\u53ef\u901a\u8fc7\u8367\u5149\u663e\u5fae\u955c\u76f4\u63a5\u89c2\u5bdf\u7ec6\u80de\u5f62\u6001\u3001\u5206\u5e03\u53ca\u589e\u6b96\u60c5\u51b5\uff0c\u9002\u7528\u4e8e\u7ec6\u80de\u8fc1\u79fb\u3001\u5171\u57f9\u517b\u3001\u4f53\u5185\u79fb\u690d\u7b49\u9700\u8ffd\u8e2a\u7684\u5b9e\u9a8c\uff1b<\/li>\n<li>\u529f\u80fd\u4fdd\u7559\uff1a\u4fdd\u7559\u539f\u4ee3 BMSCs \u7684\u591a\u5411\u5206\u5316\u6f5c\u80fd\u4e0e\u9020\u8840\u652f\u6301\u529f\u80fd\uff0c\u53ef\u7528\u4e8e\u7ec4\u7ec7\u5de5\u7a0b\u79cd\u5b50\u7ec6\u80de\u7814\u7a76\u3001\u9020\u8840\u5fae\u73af\u5883\u673a\u5236\u63a2\u7d22\u3001\u5e72\u7ec6\u80de\u6cbb\u7597\u76f8\u5173\u9884\u5b9e\u9a8c\u3002<\/li>\n<\/ol>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u5b9e\u9a8c\u5e94\u7528\u65b9\u5411<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u4f53\u5916\u7814\u7a76\uff1a\u9aa8\u9ad3\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u591a\u5411\u5206\u5316\u673a\u5236\uff08\u5982\u6210\u9aa8\u3001\u6210\u8102\u8bf1\u5bfc\u5b9e\u9a8c\uff09\u3001\u9020\u8840\u652f\u6301\u56e0\u5b50\u5206\u6ccc\u68c0\u6d4b\u3001\u5e72\u7ec6\u80de\u4e0e\u5176\u4ed6\u7ec6\u80de\uff08\u5982\u9020\u8840\u5e72\u7ec6\u80de\uff09\u5171\u57f9\u517b\u7814\u7a76\uff1b<\/li>\n<li>\u4f53\u5185\u7814\u7a76\uff1a\u5e72\u7ec6\u80de\u4f53\u5185\u79fb\u690d\u540e\u7684\u5206\u5e03\u4e0e\u5b58\u6d3b\u8ffd\u8e2a\uff08\u5229\u7528 GFP \u8367\u5149\u4fe1\u53f7\uff09\u3001\u7ec4\u7ec7\u635f\u4f24\u4fee\u590d\u6548\u679c\u8bc4\u4f30\u3001\u514d\u75ab\u8c03\u8282\u529f\u80fd\u7814\u7a76\u3002<\/li>\n<\/ul>\n<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u7ec6\u80de\u57f9\u517b\u6838\u5fc3\u4fe1\u606f<\/h1>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57fa\u7840\u53c2\u6570<\/h2>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff0c\u53ef\u901a\u8fc7\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u6216\u4f9b\u5e94\u5546\u83b7\u53d6\u8be6\u7ec6\u6eaf\u6e90\u4fe1\u606f\uff0c\u786e\u4fdd\u7ec6\u80de\u682a\u7a33\u5b9a\u6027\u3001\u6c38\u751f\u5316\u7279\u6027\u53ca\u9aa8\u9ad3\u95f4\u5145\u8d28\u5e72\u7ec6\u80de\u5c5e\u6027\u53ef\u9760\u3002<\/div>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u4e0e\u51bb\u5b58<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u6761\u4ef6<\/h3>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u5e38\u89c4 CO\u2082\u57f9\u517b\u73af\u5883\u5373\u53ef\uff0c\u9700\u786e\u4fdd\u57f9\u517b\u7bb1\u6e29\u5ea6\u3001\u6e7f\u5ea6\u7a33\u5b9a\uff0c\u907f\u514d\u73af\u5883\u6ce2\u52a8\u5f71\u54cd\u7ec6\u80de\u589e\u6b96\u4e0e\u5206\u5316\u6f5c\u80fd\uff09\uff1b<\/div>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u64cd\u4f5c\u6ce8\u610f\u4e8b\u9879\uff08\u9488\u5bf9\u6c38\u751f\u5316\u5e72\u7ec6\u80de\u7279\u6027\uff09<\/h3>\n<ol class=\"auto-hide-last-sibling-br\">\n<li>\u4f20\u4ee3\u64cd\u4f5c\uff1a\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u7ec6\u80de\u8d34\u58c1\u751f\u957f\u81f3\u5bc6\u5ea6 80%-90% \u65f6\u4f20\u4ee3\uff08\u5bc6\u5ea6\u8fc7\u4f4e\u6613\u5bfc\u81f4\u7ec6\u80de\u751f\u957f\u7f13\u6162\uff0c\u8fc7\u9ad8\u6613\u51fa\u73b0\u63a5\u89e6\u6291\u5236\uff09\uff1b<\/li>\n<li>\u7528\u80f0\u9176\u6e29\u548c\u6d88\u5316\uff0c\u5f85\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\u5f62\u6001\u8fb9\u7f18\u6536\u7f29\u3001\u7ec6\u80de\u95f4\u9699\u589e\u5927\u540e\uff0c\u7acb\u5373\u52a0\u4e13\u7528\u57f9\u517b\u57fa\u7ec8\u6b62\u6d88\u5316\uff0c\u8f7b\u67d4\u5439\u6253\u5206\u6563\u7ec6\u80de\uff08\u907f\u514d\u5267\u70c8\u5439\u6253\u635f\u4f24\u7ec6\u80de\uff0c\u5f71\u54cd\u5206\u5316\u529f\u80fd\uff09\uff1b<\/li>\n<li>\u4f20\u4ee3\u6bd4\u4f8b\u5efa\u8bae 1:3-1:5\uff0c\u786e\u4fdd\u4f20\u4ee3\u540e\u7ec6\u80de\u5bc6\u5ea6\u9002\u5b9c\uff0c\u7ef4\u6301\u5bf9\u6570\u751f\u957f\u671f\u3002<\/li>\n<\/ul>\n<\/li>\n<li>GFP \u4fdd\u62a4\uff1aGFP \u5bf9\u5149\u654f\u611f\uff0c\u907f\u514d\u957f\u65f6\u95f4\u5f3a\u5149\u76f4\u5c04\u57f9\u517b\u74f6\uff0c\u89c2\u5bdf\u8367\u5149\u65f6\u7f29\u77ed\u66b4\u9732\u65f6\u95f4\uff0c\u51cf\u5c11\u8367\u5149\u4fe1\u53f7\u6dec\u706d\uff1b<\/li>\n<li>\u5206\u5316\u5b9e\u9a8c\u9002\u914d\uff1a\u8fdb\u884c\u6210\u9aa8\u3001\u6210\u8102\u7b49\u5206\u5316\u8bf1\u5bfc\u524d\uff0c\u9700\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff0c\u4e14\u786e\u4fdd\u7ec6\u80de\u4f20\u4ee3\u6b21\u6570\u4e0d\u8d85\u8fc7 10 \u4ee3\uff0c\u907f\u514d\u957f\u671f\u57f9\u517b\u5bfc\u81f4\u5206\u5316\u6f5c\u80fd\u4e0b\u964d\uff1b<\/li>\n<li>\u57f9\u517b\u57fa\u7ba1\u7406\uff1a\u4e13\u7528\u57f9\u517b\u57fa\u9700\u6309\u8bf4\u660e\u4e66\u8981\u6c42\u50a8\u5b58\uff08\u5982\u907f\u5149\u51b7\u85cf\uff09\uff0c\u4f7f\u7528\u524d\u5e73\u8861\u81f3\u5ba4\u6e29\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u5bfc\u81f4\u6210\u5206\u5931\u6548\u3002<\/li>\n<\/ol>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u51bb\u5b58\u4e0e\u590d\u82cf<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u51bb\u5b58\u6db2\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff1b<\/li>\n<li>\u51bb\u5b58\u8981\u70b9\uff1a\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u3001\u72b6\u6001\u826f\u597d\u7684\u7ec6\u80de\uff08GFP \u8367\u5149\u5747\u5300\u3001\u65e0\u5206\u5316\u8ff9\u8c61\uff09\uff0c\u80f0\u9176\u6d88\u5316\u6536\u96c6\u540e\uff0c\u7528\u4e13\u7528\u57f9\u517b\u57fa\u6e05\u6d17 1 \u6b21\uff0c\u518d\u7528\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u7ec6\u80de\u5bc6\u5ea6\u81f3 2\u00d710\u2076-5\u00d710\u2076cells\/ml\uff1b\u68af\u5ea6\u964d\u6e29\uff084\u2103 30 \u5206\u949f\u2192-20\u2103 1 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u957f\u671f\u4fdd\u5b58\uff09\uff0c\u907f\u514d\u964d\u6e29\u8fc7\u5feb\u635f\u4f24\u7ec6\u80de\uff1b<\/li>\n<li>\u590d\u82cf\u8981\u70b9\uff1a\u4ece\u6db2\u6c2e\u53d6\u51fa\u51bb\u5b58\u7ba1\u540e\uff0c\u7acb\u5373\u653e\u5165 37\u2103\u6c34\u6d74\u5feb\u901f\u878d\u5316\uff08\u7ea6 1-2 \u5206\u949f\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\uff09\uff1b1000rpm \u79bb\u5fc3 5 \u5206\u949f\u5f03\u51bb\u5b58\u6db2\uff0c\u7528\u9884\u70ed\u81f3 37\u2103\u7684\u4e13\u7528\u57f9\u517b\u57fa\u91cd\u60ac\u7ec6\u80de\uff1b\u590d\u82cf\u540e\u7ec6\u80de\u8d34\u58c1\u8f83\u6162\uff0c\u9700\u9759\u7f6e 24-48h \u518d\u89c2\u5bdf\uff0c\u4f18\u5148\u786e\u8ba4\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\u3001\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\u5f62\u6001\u53ca GFP \u8367\u5149\u5f3a\u5ea6\uff08\u8367\u5149\u5747\u5300\u65e0\u8870\u51cf\u4e3a\u72b6\u6001\u6b63\u5e38\uff09\u3002<\/li>\n<\/ul>\n<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u8d27\u671f\u4e0e\u8fd0\u8f93<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff1b<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ 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