{"id":1453,"date":"2025-10-05T23:50:47","date_gmt":"2025-10-05T15:50:47","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&p=1453"},"modified":"2025-10-05T23:50:48","modified_gmt":"2025-10-05T15:50:48","slug":"thp-1-isre-nluc-puro","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/thp-1-isre-nluc-puro\/","title":{"rendered":"THP-1-ISRE-Nluc-puro"},"content":{"rendered":"

\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n
\u7ec6\u80de\u540d\u79f0\uff1aTHP-1-ISRE-Nluc-puro, \u4eba\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u7ec6\u80de\uff08ISRE \u4e3a\u5e72\u6270\u7d20\u523a\u6fc0\u53cd\u5e94\u5143\u4ef6\uff0cNLUC \u4e3a\u7eb3\u7c73\u8367\u5149\u7d20\u9176\uff0c\u53ef\u76d1\u6d4b ISRE \u901a\u8def\u6d3b\u6027\uff1b\u5e26 puro \u6297\u6027\uff09<\/p>\n
<\/div>\n

\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/p>\n

<\/div>\n

\u7ec4\u7ec7\u6765\u6e90\uff1a\u6025\u6027\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5<\/p>\n

<\/div>\n

\u751f\u957f\u7279\u6027\uff1a\u60ac\u6d6e\u751f\u957f\uff08\u65e0\u9700\u8d34\u58c1\uff0c\u5173\u6ce8\u5bc6\u5ea6\u4e0e\u5206\u6563\u72b6\u6001\uff09<\/p>\n

<\/div>\n

\u7ec6\u80de\u5f62\u6001\uff1a\u5706\u5f62\uff0c\u5355\u4e2a\u6216\u5448\u7247<\/p>\n

<\/div>\n

\u7279\u522b\u6ce8\u610f\uff1apuro \u836f\u7b5b\u6d53\u5ea6 1.0\u03bcg\/ml\uff08\u53bb\u9664\u672a\u8f6c\u67d3\u7ec6\u80de\uff09\uff0c\u57f9\u517b\u7528 0.5\u03bcg\/ml \u7ef4\u6301\uff1b\u57f9\u517b\u57fa\u9700\u52a0 0.05 mM 2 – \u5def\u57fa\u4e59\u9187\uff08\u5fc5\u9700\u6210\u5206\uff0c\u4e0d\u53ef\u7701\u7565\uff09<\/p>\n

<\/div>\n

\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4f20\u4ee3\uff0c\u4fdd ISRE \u901a\u8def\u54cd\u5e94\u6027\u3001NLUC \u6d3b\u6027\u53ca\u767d\u8840\u75c5\u7ec6\u80de\u7279\u6027\uff09<\/p>\n

<\/div>\n

\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u74f6\u6216 1mL \u51bb\u5b58\u7ba1<\/p>\n

<\/div>\n

\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u9634\u6027\uff08\u65e0\u6c61\u67d3\uff0c\u4fdd\u969c\u5b9e\u9a8c\u51c6\u786e\u6027\uff09<\/p><\/div>\n

\u80cc\u666f\u4e0e\u6838\u5fc3\u4ef7\u503c<\/h1>\n

\u80cc\u666f\u4ecb\u7ecd<\/h2>\n
THP-1-ISRE-Nluc-puro \u7ec6\u80de\u662f THP-1 \u4eba\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u7ec6\u80de\u6784\u5efa\u7684 ISRE \u901a\u8def\u62a5\u544a\u7ec6\u80de\u682a\uff0c\u7a33\u5b9a\u6574\u5408 ISRE \u8c03\u63a7\u7684 NLUC \u62a5\u544a\u57fa\u56e0\u5e76\u5e26 puro \u6297\u6027\u3002\u53ef\u901a\u8fc7 NLUC \u8367\u5149\u5f3a\u5ea6\u53d8\u5316\uff0c\u5b9e\u65f6\u91cf\u5316 ISRE \u4fe1\u53f7\u901a\u8def\u7684\u6fc0\u6d3b\u6216\u6291\u5236\u60c5\u51b5\uff0c\u9002\u7528\u4e8e I \u578b\u5e72\u6270\u7d20\uff08INF-\u03b1\uff09\u76f8\u5173\u514d\u75ab\u5e94\u7b54\u3001\u6297\u75c5\u6bd2\u53cd\u5e94\u3001\u708e\u75c7\u673a\u5236\u7814\u7a76\uff0c\u53ca\u9776\u5411 ISRE \u901a\u8def\u7684\u836f\u7269\u7b5b\u9009\u3002<\/div>\n

\u5173\u952e\u7279\u6027<\/h2>\n
    \n
  1. \u901a\u8def\u62a5\u544a\u529f\u80fd\uff1aINF-\u03b1 \u8bf1\u5bfc ISRE \u901a\u8def\u6fc0\u6d3b\u65f6\uff0cNLUC \u8868\u8fbe\u589e\u5f3a\uff0c\u8367\u5149\u4fe1\u53f7\u53ef\u7cbe\u51c6\u53cd\u6620\u901a\u8def\u6d3b\u6027\uff1b<\/li>\n
  2. \u7ec6\u80de\u7279\u6027\uff1a\u4fdd\u7559 THP-1 \u7ec6\u80de\u5355\u6838\u7ec6\u80de\u5c5e\u6027\uff0c\u53ef\u9002\u914d\u5355\u6838\u7ec6\u80de\u5206\u5316\u3001\u514d\u75ab\u8c03\u63a7\u7b49\u5173\u8054\u5b9e\u9a8c\uff0c\u62d3\u5bbd\u7814\u7a76\u573a\u666f\u3002<\/li>\n<\/ol>\n

    \u57f9\u517b\u4e0e\u51bb\u5b58<\/h1>\n

    \u57fa\u7840\u4fe1\u606f<\/h2>\n
    \u57f9\u517b\u57fa\uff1aRPMI-1640+10% FBS+PS+0.05 mM 2 – \u5def\u57fa\u4e59\u9187\uff0c\u6309\u9700\u6dfb\u52a0 0.5\u03bcg\/ml puro\uff1b<\/p>\n
    <\/div>\n

    \u57f9\u517b\u6761\u4ef6\uff1a95% \u7a7a\u6c14 + 5% CO\u2082\uff0c37\u2103\uff0c\u6e7f\u5ea6 90% \u5de6\u53f3\uff1b<\/p>\n

    <\/div>\n

    \u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff08\u63a8\u8350 IMC-701\uff09\uff0c\u6db2\u6c2e\u50a8\u5b58\u3002<\/p><\/div>\n

    \u64cd\u4f5c\u8981\u70b9<\/h2>\n
      \n
    1. \u5bc6\u5ea6\u63a7\u5236\uff1a\u9002\u5b9c\u5bc6\u5ea6 5\u00d710\u2075-1\u00d710\u2076cells\/mL\uff0c\u6bcf\u65e5\u76d1\u6d4b\uff0c\u5bc6\u5ea6\u8fbe\u4e0a\u9650\u65f6\u6309 1:2-1:3 \u6bd4\u4f8b\u7a00\u91ca\u4f20\u4ee3\uff0c\u907f\u514d\u8fc7\u5ea6\u5bc6\u96c6\u5bfc\u81f4\u6d3b\u6027\u4e0b\u964d\uff1b<\/li>\n
    2. 2 – \u5def\u57fa\u4e59\u9187\u7ba1\u7406\uff1a\u9700\u65b0\u9c9c\u6dfb\u52a0\u81f3\u57f9\u517b\u57fa\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u5931\u6548\uff0c\u4fdd\u969c\u7ec6\u80de\u6b63\u5e38\u751f\u957f\uff1b<\/li>\n
    3. INF-\u03b1 \u6fc0\u6d3b\u5b9e\u9a8c\uff1a\u5f00\u5c55\u901a\u8def\u9a8c\u8bc1\u65f6\uff0c\u6309\u5b9e\u9a8c\u65b9\u6848\u52a0\u5165 INF-\u03b1 \u8bf1\u5bfc\uff0c\u901a\u8fc7\u8367\u5149\u68c0\u6d4b\u4eea\u5668\u76d1\u6d4b NLUC \u4fe1\u53f7\u53d8\u5316\uff0c\u786e\u8ba4\u901a\u8def\u54cd\u5e94\u6027\uff1b<\/li>\n
    4. \u51bb\u5b58\uff1a\u53d6\u5bf9\u6570\u671f\u7ec6\u80de\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\uff0c\u7528\u542b 2 – \u5def\u57fa\u4e59\u9187\u3001puro \u7684\u57f9\u517b\u57fa\u6e05\u6d17\u540e\u91cd\u60ac\uff0c\u5bc6\u5ea6\u8c03\u81f3 2\u00d710\u2076-5\u00d710\u2076cells\/ml\uff0c\u68af\u5ea6\u964d\u6e29\u51bb\u5b58\uff1b<\/li>\n
    5. \u590d\u82cf\uff1a37\u2103\u6c34\u6d74\u901f\u878d\uff0c\u79bb\u5fc3\u5f03\u51bb\u5b58\u6db2\uff0c\u7528\u65b0\u9c9c\u57f9\u517b\u57fa\u91cd\u60ac\uff1b24h \u540e\u68c0\u6d4b\u7ec6\u80de\u5b58\u6d3b\u7387\uff08\u53f0\u76fc\u84dd\u67d3\u8272\uff0c\u5b58\u6d3b\u7387\u226580%\uff09\uff0c\u540e\u7eed\u53ef\u901a\u8fc7 INF-\u03b1 \u6fc0\u6d3b\u5b9e\u9a8c\u9a8c\u8bc1\u901a\u8def\u529f\u80fd\u3002<\/li>\n<\/ol>\n

      \u8d27\u671f\u4e0e\u8fd0\u8f93<\/h1>\n
      \u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff1b<\/p>\n
      <\/div>\n

      \u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\uff0c\u514d\u8fd0\u8d39\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u52a0\u5e72\u51b0\uff0c\u9700\u4ed8\u8d39\uff09\uff0c\u987a\u4e30\uff1b<\/p>\n

      <\/div>\n

      \u8fd0\u8f93\u6ce8\u610f\uff1a\u590d\u82cf\u53d1\u8d27\u7528\u9632\u9707\u5305\u88c5\u51cf\u5c11\u9707\u8361\uff0c\u907f\u514d\u7ec6\u80de\u805a\u96c6\uff1b\u51bb\u5b58\u53d1\u8d27\u786e\u4fdd\u5e72\u51b0\u5145\u8db3\uff0c\u7ef4\u6301\u4f4e\u6e29\u72b6\u6001\u3002<\/p><\/div>\n

      \u4f9b\u5e94\u9650\u5236<\/h1>\n
      \u4ec5\u9650\u79d1\u5b66\u7814\u7a76\uff0c\u4e0d\u53ef\u7528\u4e8e\u52a8\u7269 \/ \u4eba\u7c7b\u75be\u75c5\u6cbb\u7597\u3002<\/div>\n","protected":false},"excerpt":{"rendered":"

      \u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aTHP-1-ISRE-Nluc-puro, <\/p>\n

      \n \u7ee7\u7eed\u9605\u8bfb <\/span><\/i><\/a>\n <\/div>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[18],"product_tag":[],"class_list":{"0":"post-1453","1":"product","2":"type-product","3":"status-publish","5":"product_cat-18","7":"first","8":"instock","9":"sale","10":"taxable","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product\/1453","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/comments?post=1453"}],"wp:attachment":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/media?parent=1453"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=1453"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=1453"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=1453"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}