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\u4ee5\u4e0a\uff0c\u4e14\u590d\u82cf\u540e\u5e72\u6270\u7d20\u654f\u611f\u6027\u3001\u81f4\u7624\u6027\u65e0\u663e\u8457\u6539\u53d8\uff0c\u786e\u4fdd\u5b9e\u9a8c\u8d77\u59cb\u6761\u4ef6\u4e00\u81f4\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u5747\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff0c\u8986\u76d6\u5e38\u89c1\u81f4\u75c5\u6027\u652f\u539f\u4f53\u7c7b\u578b\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u53ef\u76f4\u63a5\u7528\u4e8e\u5b9e\u9a8c\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u751f\u957f\u3001\u5e72\u6270\u7d20\u4f5c\u7528\u6548\u679c\u53ca\u81f4\u7624\u6027\u5b9e\u9a8c\u7ed3\u679c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CRL-1611\uff0cECACC; 88100508\uff1b\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u57fa\u7840\u533b\u5b66\u7814\u7a76\u6240\u7ec6\u80de\u8d44\u6e90\u4e2d\u5fc3\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\u3001ECACC\uff09\u4e0e\u56fd\u5185\u673a\u6784\u53cc\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u57fa\u56e0\u578b\u53ca\u751f\u7269\u5b66\u7279\u6027\u7ecf\u8fc7\u591a\u91cd\u9a8c\u8bc1\uff0c\u4e0e A-704\uff08\u5efa\u8bae\u8865\u5145\u4fdd\u85cf\uff09\u3001A498 \u7684\u4fdd\u85cf\u673a\u6784\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5b9e\u9a8c\u7684\u6eaf\u6e90\u7ba1\u7406\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a90% DMEM+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff0c\u914d\u65b9\u8bf4\u660e\uff1a\u2460\u57f9\u517b\u57fa\u9002\u914d\u6027\uff1a\u91c7\u7528 DMEM \u57f9\u517b\u57fa\uff0c\u4e0e A-704 \u7684 DMEM \u914d\u65b9\u4e00\u81f4\uff0c\u533a\u522b\u4e8e A498 \u7684 MEM\u3001786-O \u7684 RPMI-1640\uff0c\u5176\u9ad8\u7cd6\u914d\u65b9\uff08\u542b 4.5g\/L \u8461\u8404\u7cd6\uff09\u53ef\u6ee1\u8db3\u8be5\u7ec6\u80de\u5feb\u901f\u589e\u6b96\u53ca\u5e72\u6270\u7d20\u5904\u7406\u540e\u7684\u80fd\u91cf\u9700\u6c42\uff1b\u246110% FBS \u5efa\u8bae\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u5e72\u6270\u7d20\u6837\u7269\u8d28\u5e72\u6270\u5b9e\u9a8c\u7ed3\u679c\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u4ee3\u8c22\u53ca\u5e72\u6270\u7d20\u4f5c\u7528\u8fc7\u7a0b\u3002\u57f9\u517b\u57fa\u9700 37\u2103\u9884\u70ed 15-20 \u5206\u949f\u540e\u4f7f\u7528\uff0c\u907f\u514d\u4f4e\u6e29\u523a\u6fc0\u7ec6\u80de\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff0c\u4e0e A-704\u3001A498\u3001786-O \u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u786e\u4fdd\u591a\u7ec6\u80de\u682a\u5b9e\u9a8c\u73af\u5883\u7684\u7edf\u4e00\u6027\uff0c\u51cf\u5c11\u73af\u5883\u56e0\u7d20\u5bf9\u5b9e\u9a8c\u7ed3\u679c\u7684\u5e72\u6270\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u500d\u589e\u65f6\u95f4\uff1a~28-36 hours\uff08\u589e\u6b96\u901f\u5ea6\u5feb\u4e8e A-704\uff08\u672a\u660e\u786e\uff0c\u63a8\u6d4b\u8f83\u6162\uff09\u3001A498\uff0860 \u5c0f\u65f6\uff09\u3001769-P\uff0835 \u5c0f\u65f6\uff09\uff0c\u4e0e 786-O\uff0824-45 \u5c0f\u65f6\uff09\u76f8\u8fd1\uff0c\u5bf9\u6570\u751f\u957f\u671f\u4e3a\u63a5\u79cd\u540e 1-2 \u5929\uff0c\u9700\u6839\u636e\u8be5\u7279\u6027\u53ca\u65f6\u4f20\u4ee3\uff08\u5efa\u8bae 2-3 \u5929\u4f20\u4ee3\u4e00\u6b21\uff09\uff0c\u907f\u514d\u7ec6\u80de\u8fc7\u5ea6\u5bc6\u96c6\u5f71\u54cd\u5e72\u6270\u7d20\u5904\u7406\u6548\u679c\uff1b\u5b9e\u9a8c\u8bbe\u8ba1\u4e2d\u53ef\u5229\u7528\u77ed\u500d\u589e\u65f6\u95f4\u4f18\u52bf\uff0c\u5f00\u5c55\u5feb\u901f\u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\uff08\u5982 48 \u5c0f\u65f6\u836f\u7269\u6d3b\u6027\u68c0\u6d4b\uff09\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u81f4\u7624\u6027\uff1aYes, in nude mice (Tumors developed within 21 days at 100% frequency (5\/5) in nude mice inoculated subcutaneously with 1\u00d710^7 cells).\uff08\u81f4\u7624\u6027\u660e\u786e\u4e14\u9ad8\u6548\uff1a\u2460\u9ad8\u6210\u7624\u7387\uff08100%\uff09\u4e0e\u77ed\u6210\u7624\u5468\u671f\uff0821 \u5929\u5185\uff09\uff0c\u9002\u5408\u5feb\u901f\u6784\u5efa\u4f53\u5185\u80bf\u7624\u6a21\u578b\uff0c\u7f29\u77ed\u5b9e\u9a8c\u5468\u671f\uff1b\u2461\u660e\u786e\u7684\u63a5\u79cd\u6761\u4ef6\uff081\u00d710^7 cells \/ \u53ea\uff0c\u76ae\u4e0b\u63a5\u79cd\uff09\uff0c\u53ef\u76f4\u63a5\u53c2\u8003\u5f00\u5c55\u5b9e\u9a8c\uff0c\u51cf\u5c11\u9884\u5b9e\u9a8c\u6210\u672c\uff1b\u4e0e A-704\uff08\u4f53\u5185\u4e0d\u6210\u7624\uff09\u5f62\u6210\u9c9c\u660e\u5bf9\u6bd4\uff0c\u53ef\u7528\u4e8e\u7814\u7a76\u80bf\u7624\u8f6c\u79fb\u7076\u4e0e\u539f\u53d1\u7076\u5728\u4f53\u5185\u6210\u7624\u80fd\u529b\u4e0a\u7684\u5dee\u5f02\u673a\u5236\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u6d41\u7a0b\u4e0e\u5176\u4ed6\u80be\u764c\u7ec6\u80de\u682a\u4e00\u81f4\uff1a\u2460\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\u6d88\u5316\u79bb\u5fc3\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 1\u00d710\u2076-5\u00d710\u2076cells\/mL\uff1b\u2461\u5206\u88c5\u540e 4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u5efa\u8bae\u7528\u7a0b\u5e8f\u964d\u6e29\u76d2\uff0c\u51cf\u5c11\u4f4e\u6e29\u635f\u4f24\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u8f6c\u5165\u6db2\u6c2e\u957f\u671f\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u5185\u5b8c\u5168\u878d\u5316\uff0c\u79bb\u5fc3\u53bb\u9664\u51bb\u5b58\u6db2\uff0c\u7528\u9884\u70ed\u7684 DMEM \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u907f\u514d DMSO \u5bf9\u7ec6\u80de\u7684\u6bd2\u6027\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u5145\u8db3\uff0c\u6240\u6709\u73b0\u8d27\u7ec6\u80de\u5747\u7ecf\u8fc7\u6d3b\u6027\u68c0\u6d4b\u53ca\u5e72\u6270\u7d20\u654f\u611f\u6027\u9884\u5b9e\u9a8c\u9a8c\u8bc1\uff0c\u786e\u4fdd\u6838\u5fc3\u7279\u6027\u8fbe\u6807\uff0c\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u7279\u6b8a\u9700\u6c42\u5982\u52a0\u6025\u63d0\u4f9b\u5e72\u6270\u7d20\u6291\u5236\u7387\u68c0\u6d4b\u62a5\u544a\uff0c\u53ef\u63d0\u524d\u4e0e\u4f9b\u5e94\u65b9\u6c9f\u901a\u534f\u8c03\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u6052\u6e29\u6ce1\u6cab\u7bb1\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u786e\u4fdd\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u6e29\u5ea6\u6ce2\u52a8\u4e0d\u8d85\u8fc7 \u00b12\u2103\uff0c\u5230\u8fbe\u540e\u5efa\u8bae\u7acb\u5373\u653e\u5165 37\u2103\u57f9\u517b\u7bb1\uff0c\u56e0\u589e\u6b96\u901f\u5ea6\u5feb\uff0c24 \u5c0f\u65f6\u540e\u5373\u53ef\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 500g-1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226550%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff0c\u4fdd\u969c\u590d\u82cf\u5b58\u6d3b\u7387\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u65e5\u8fbe\u670d\u52a1\uff0c\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca\u5e72\u6270\u7d20\u654f\u611f\u6027\u6539\u53d8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff0c\u4f7f\u7528\u9700\u7b26\u5408\u300a\u4f53\u5916\u57f9\u517b\u52a8\u7269\u7ec6\u80de\u7cfb\u7ba1\u7406\u89c4\u8303\u300b\u300a\u533b\u5b66\u79d1\u7814\u4f26\u7406\u5ba1\u67e5\u529e\u6cd5\u300b\u7b49\u76f8\u5173\u6cd5\u89c4\u53ca\u4f26\u7406\u8981\u6c42\uff0c\u4e0e\u5176\u4ed6\u80be\u764c\u7ec6\u80de\u682a\u4f9b\u5e94\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4fbf\u4e8e\u5b9e\u9a8c\u5ba4\u5408\u89c4\u7ba1\u7406\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460\u5e72\u6270\u7d20\u76f8\u5173\u5b9e\u9a8c\u4f18\u5316\uff1a\u4f5c\u4e3a\u5e72\u6270\u7d20\u7814\u7a76\u4e13\u5c5e\u6a21\u578b\uff0c\u5b9e\u9a8c\u65f6\u9700\u8bbe\u7f6e\u68af\u5ea6\u5e72\u6270\u7d20\u6d53\u5ea6\uff08\u5982 0\u300110\u300150\u3001100\u3001500IU\/mL\uff09\uff0c\u5904\u7406\u65f6\u95f4\u5efa\u8bae\u4e3a 48-72 \u5c0f\u65f6\uff08\u7ed3\u5408 28-36 \u5c0f\u65f6\u500d\u589e\u65f6\u95f4\uff09\uff0c\u901a\u8fc7 CCK-8 \u6216 MTT \u6cd5\u68c0\u6d4b\u7ec6\u80de\u6d3b\u529b\uff0c\u8ba1\u7b97\u6291\u5236\u7387\uff1b\u540c\u65f6\u8bbe\u7f6e\u65e0\u5e72\u6270\u7d20\u7684\u7a7a\u767d\u5bf9\u7167\u53ca\u5df2\u77e5\u5e72\u6270\u7d20\u654f\u611f\u7ec6\u80de\uff08\u5982 HeLa\uff09\u4f5c\u4e3a\u9633\u6027\u5bf9\u7167\uff0c\u786e\u4fdd\u5b9e\u9a8c\u4f53\u7cfb\u6709\u6548\u6027\uff1b\u2461\u8f6c\u79fb\u76f8\u5173\u5b9e\u9a8c\u64cd\u4f5c\uff1a\u4f9d\u6258\u80f8\u6c34\u8f6c\u79fb\u7076\u6765\u6e90\u7279\u6027\uff0c\u53ef\u5f00\u5c55 Transwell \u4fb5\u88ad \/ \u8fc1\u79fb\u5b9e\u9a8c\uff0c\u63a5\u79cd\u7ec6\u80de\u5bc6\u5ea6\u5efa\u8bae\u4e3a 5\u00d710\u2074cells \/ \u5b54\uff08Transwell \u5c0f\u5ba4\uff09\uff0c\u57f9\u517b 24-36 \u5c0f\u65f6\u540e\u8ba1\u6570\u7a7f\u819c\u7ec6\u80de\uff0c\u8bc4\u4f30\u7ec6\u80de\u8f6c\u79fb\u80fd\u529b\uff1b\u5b9e\u9a8c\u4e2d\u53ef\u4e0e A-704\uff08\u539f\u53d1\u7076\uff09\u5bf9\u6bd4\uff0c\u5206\u6790\u8f6c\u79fb\u7076\u4e0e\u539f\u53d1\u7076\u5728\u4fb5\u88ad\u80fd\u529b\u4e0a\u7684\u5dee\u5f02\uff1b\u2462\u81f4\u7624\u6027\u5b9e\u9a8c\u8bbe\u8ba1\uff1a\u5f00\u5c55\u88f8\u9f20\u6210\u7624\u5b9e\u9a8c\u65f6\uff0c\u4e25\u683c\u53c2\u7167\u63a8\u8350\u63a5\u79cd\u6761\u4ef6\uff081\u00d710^7 cells \/ \u53ea\uff0c\u76ae\u4e0b\u63a5\u79cd\uff09\uff0c\u9009\u62e9 6-8 \u5468\u9f84\u88f8\u9f20\uff0c\u63a5\u79cd\u540e\u6bcf\u5468\u6d4b\u91cf\u80bf\u7624\u4f53\u79ef\uff08\u957f \u00d7 \u5bbd \u00b2\/2\uff09\uff0c21 \u5929\u5185\u5373\u53ef\u89c2\u5bdf\u5230\u660e\u663e\u80bf\u7624\uff0c\u9002\u5408\u5feb\u901f\u8bc4\u4f30\u836f\u7269\u4f53\u5185\u6297\u7624\u6548\u679c\uff1b\u2463STR \u6027\u522b\u4f4d\u70b9\u5dee\u5f02\u5904\u7406\uff1a\u56e0 Amelogenin \u4f4d\u70b9\uff08X\uff09\u4e0e\u6027\u522b\u6807\u6ce8\uff08\u7537\uff09\u5b58\u5728\u77db\u76fe\uff0c\u5efa\u8bae\u590d\u82cf\u540e\u901a\u8fc7\u67d3\u8272\u4f53\u6838\u578b\u5206\u6790\uff08\u5982 G \u5e26\u67d3\u8272\uff09\u6216 Amelogenin \u57fa\u56e0\u5355\u72ec PCR \u9a8c\u8bc1\uff0c\u786e\u8ba4\u7ec6\u80de\u5b9e\u9645\u6027\u522b\uff0c\u907f\u514d\u56e0\u6027\u522b\u8bef\u5dee\u5f71\u54cd\u5b9e\u9a8c\u8bbe\u8ba1\uff08\u5982\u6027\u522b\u76f8\u5173\u836f\u7269\u4ee3\u8c22\u7814\u7a76\uff09\uff1b\u2464\u57f9\u517b\u4e0e\u4f20\u4ee3\u6ce8\u610f\u4e8b\u9879\uff1a\u56e0\u589e\u6b96\u901f\u5ea6\u5feb\uff0c\u9700\u6bcf\u5929\u955c\u68c0\u7ec6\u80de\u5bc6\u5ea6\uff0c\u5f85\u5bc6\u5ea6\u8fbe 70%-80% \u65f6\u7acb\u5373\u4f20\u4ee3\uff1b\u6d88\u5316\u65f6\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 1-2 \u5206\u949f\uff08\u77ed\u4e8e A498\u3001A-704\uff09\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff0c\u9632\u6b62\u5f71\u54cd\u540e\u7eed\u8f6c\u79fb\u5b9e\u9a8c\u6216\u8f6c\u67d3\u6548\u7387\uff1b\u2465\u6c61\u67d3\u9632\u63a7\uff1a\u5b9e\u9a8c\u64cd\u4f5c\u9700\u5728\u8d85\u51c0\u5de5\u4f5c\u53f0\u5185\u8fdb\u884c\uff0c\u5e72\u6270\u7d20\u5b9e\u9a8c\u4e2d\u4f7f\u7528\u7684\u8bd5\u5242\u3001\u8017\u6750\u9700\u65e0\u83cc\u5904\u7406\uff0c\u907f\u514d\u4ea4\u53c9\u6c61\u67d3\uff1b\u5b9a\u671f\u5bf9 DMEM \u57f9\u517b\u57fa\u3001\u57f9\u517b\u7bb1\u8fdb\u884c\u65e0\u83cc\u68c0\u6d4b\uff0c\u786e\u4fdd\u5b9e\u9a8c\u73af\u5883\u6d01\u51c0\uff0c\u4fdd\u969c\u5b9e\u9a8c\u7ed3\u679c\u53ef\u9760\u3002<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"24\" data-line=\"true\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027 \u7ec6\u80de\u540d\u79f0\uff1aACHN 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