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    \n
  1. \u4f20\u4ee3\uff1a\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%-80% \u65f6\uff0c\u7528\u80f0\u9176\u6e29\u548c\u6d88\u5316\uff0c\u52a0 DMEM \u57f9\u517b\u57fa\u7ec8\u6b62\uff1b\u64cd\u4f5c\u65f6\u907f\u514d\u5267\u70c8\u5439\u6253\uff0c\u9632\u6b62\u4e0a\u76ae\u7ec6\u80de\u6837\u5f62\u6001\u88ab\u7834\u574f\uff0c\u4f20\u4ee3\u6bd4\u4f8b 1:3-1:5\uff0c\u4fdd\u969c\u7ec6\u80de\u540e\u7eed\u589e\u6b96\u7b26\u5408\u5176\u6076\u6027\u80bf\u7624\u7ec6\u80de\u7684\u751f\u957f\u7279\u6027\u3002<\/li>\n
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