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CRL-9520\uff1b\u4e2d\u56fd\u79d1\u5b66\u9662\u5206\u5b50\u7ec6\u80de\u79d1\u5b66\u5353\u8d8a\u521b\u65b0\u4e2d\u5fc3\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\uff09\u4e0e\u56fd\u5185\u9876\u5c16\u673a\u6784\u53cc\u91cd\u4fdd\u85cf\uff0cATCC \u7f16\u53f7 CRL-9520 \u4e3a\u8be5\u7ec6\u80de\u4e13\u5c5e\u6807\u8bc6\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u6c38\u751f\u5316\u7279\u6027\u53ca\u6b63\u5e38\u4e0a\u76ae\u529f\u80fd\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4e0e RT4\uff08\u591a\u673a\u6784\u4fdd\u85cf\uff09\u3001HK-2\uff08ATCC \u4fdd\u85cf\uff09\u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u8de8\u5730\u57df\u5b9e\u9a8c\u534f\u4f5c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1aF12K+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u91c7\u7528 F12K \u57f9\u517b\u57fa\uff0c\u533a\u522b\u4e8e RT4 \u7684 MCCOY&#8217;S 5A\u3001RT112 \u7684 RPMI-1640\u3001HK-2 \u7684\u4e13\u7528\u57f9\u517b\u57fa\uff1bF12K \u57f9\u517b\u57fa\u542b\u4e30\u5bcc\u7684\u5fae\u91cf\u5143\u7d20\u53ca\u751f\u957f\u56e0\u5b50\uff0c\u9002\u914d\u8f93\u5c3f\u7ba1\u4e0a\u76ae\u7ec6\u80de\u7684\u4ee3\u8c22\u9700\u6c42\uff0c\u53ef\u7ef4\u6301\u5176\u6b63\u5e38\u5206\u5316\u8868\u578b\uff1b10% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u75c5\u6bd2\u6216\u5206\u5316\u5e72\u6270\u56e0\u5b50\u5f71\u54cd\u7ec6\u80de\u72b6\u6001\uff1bPS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u6b63\u5e38\u4ee3\u8c22\u53ca SV40 T \u6297\u539f\u7a33\u5b9a\u8868\u8fbe\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u4e0e RT4\u3001RT112\u3001HK-2 \u7b49\u6ccc\u5c3f\u751f\u6b96\u7cfb\u7edf\u76f8\u5173\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u786e\u4fdd \u201c\u8de8\u5668\u5b98\u201d\u201c\u6b63\u5e38 &#8211; \u6076\u6027\u201d \u5bf9\u7167\u5b9e\u9a8c\u73af\u5883\u7684\u7edf\u4e00\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u6d41\u7a0b\uff1a\u2460\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\u6d88\u5316\u79bb\u5fc3\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 1\u00d710\u2076-5\u00d710\u2076cells\/mL\uff1b\u2461\u5206\u88c5\u540e 4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u5efa\u8bae\u7528\u7a0b\u5e8f\u964d\u6e29\u76d2\uff0c\u51cf\u5c11\u4f4e\u6e29\u5bf9\u7ec6\u80de\u5206\u5316\u8868\u578b\u7684\u5f71\u54cd\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u8f6c\u5165\u6db2\u6c2e\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u5185\u878d\u5316\uff0c\u79bb\u5fc3\u53bb\u9664\u51bb\u5b58\u6db2\uff0c\u7528\u9884\u70ed\u7684 F12K \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u907f\u514d DMSO \u5bf9\u7ec6\u80de\u7684\u6bd2\u6027\u5f71\u54cd\u53ca\u666e\u901a\u57f9\u517b\u57fa\u5bf9\u7ec6\u80de\u8868\u578b\u7684\u5e72\u6270\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u81f4\u7624\u6027\uff1aNo, nude mice.\uff08\u660e\u786e\u7684\u88f8\u9f20\u65e0\u81f4\u7624\u6027\uff0c\u4e0e RT4\uff08\u6761\u4ef6\u6027\u81f4\u7624\uff09\u3001RT112\uff08\u88f8\u9f20\u81f4\u7624\uff09\u5f62\u6210 \u201c\u65e0\u81f4\u7624 &#8211; \u6761\u4ef6\u81f4\u7624 &#8211; \u6076\u6027\u81f4\u7624\u201d \u68af\u5ea6\uff0c\u662f\u9a8c\u8bc1\u7ec6\u80de\u6b63\u5e38\u5c5e\u6027\u3001\u6392\u9664\u6076\u6027\u6c61\u67d3\u7684\u5173\u952e\u4f9d\u636e\uff0c\u9002\u5408\u4f5c\u4e3a\u80bf\u7624\u7814\u7a76\u4e2d\u7684\u6b63\u5e38\u5bf9\u7167\uff0c\u8bc4\u4f30\u836f\u7269\u5bf9\u6b63\u5e38\u7ec6\u80de\u7684\u6bd2\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff1auroepithelial keratins\uff08\u5c3f\u8def\u4e0a\u76ae\u89d2\u86cb\u767d\uff0c\u8f93\u5c3f\u7ba1\u4e0a\u76ae\u7279\u5f02\u6027\u6807\u5fd7\u7269\uff0c\u9a8c\u8bc1\u7ec6\u80de\u8f93\u5c3f\u7ba1\u4e0a\u76ae\u8eab\u4efd\uff09; SV40 T antigen\uff08SV40 \u75c5\u6bd2 T \u6297\u539f\uff0c\u6c38\u751f\u5316\u5173\u952e\u86cb\u767d\uff0c\u786e\u4fdd\u7ec6\u80de\u6c38\u751f\u5316\u7279\u6027\u7a33\u5b9a\uff09\uff08\u57fa\u56e0\u8868\u8fbe\u8986\u76d6\u7ec6\u80de\u8eab\u4efd\u6807\u5fd7\u7269\u4e0e\u6c38\u751f\u5316\u6807\u5fd7\u7269\uff0c\u53ef\u901a\u8fc7 Western Blot \u6216\u514d\u75ab\u8367\u5149\u6cd5\u68c0\u6d4b\uff0c\u9a8c\u8bc1\u7ec6\u80de\u8868\u578b\u7a33\u5b9a\u6027\uff0c\u907f\u514d\u957f\u671f\u4f20\u4ee3\u5bfc\u81f4\u7279\u6027\u6f02\u79fb\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u5145\u8db3\uff0c\u6240\u6709\u73b0\u8d27\u7ec6\u80de\u5747\u7ecf\u8fc7 F12K \u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001\u5c3f\u8def\u4e0a\u76ae\u89d2\u86cb\u767d\u53ca SV40 T \u6297\u539f\u8868\u8fbe\u9a8c\u8bc1\uff0c\u786e\u4fdd\u6b63\u5e38\u8f93\u5c3f\u7ba1\u4e0a\u76ae\u6c38\u751f\u5316\u7279\u6027\u7a33\u5b9a\uff0c\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u53ef\u4e0e RT4\u3001RT112 \u540c\u6b65\u4e0b\u5355\uff0c\u4fdd\u969c \u201c\u6b63\u5e38 &#8211; 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