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data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9700\u9002\u914d\u8d34\u58c1\u7f13\u6162\u7279\u6027\uff1a\u2460\u9009\u62e9\u5b8c\u5168\u8d34\u58c1\u5c55\u5f00\u7684\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u4f20\u4ee3\u540e 72 \u5c0f\u65f6\u5de6\u53f3\uff09\uff0c\u907f\u514d\u4f7f\u7528\u672a\u5b8c\u5168\u8d34\u58c1\u7684\u7ec6\u80de\uff1b\u2461\u6d88\u5316\u65f6\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 3-4 \u5206\u949f\uff08\u957f\u4e8e T24 \u7684 1-2 \u5206\u949f\uff09\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u3001\u8fb9\u7f18\u6536\u7f29\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u635f\u4f24\u7ec6\u80de\u8868\u9762\u9ecf\u9644\u7ed3\u6784\uff1b\u2462\u91cd\u60ac\u6d53\u5ea6\u8c03\u6574\u81f3 2\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e\u5e38\u89c4\u7ec6\u80de\uff09\uff0c\u63d0\u5347\u590d\u82cf\u540e\u8d34\u58c1\u7ec6\u80de\u5bc6\u5ea6\uff1b\u51bb\u5b58\u6d41\u7a0b\u4e0e T24 \u4e00\u81f4\uff0c\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 DMEM \u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\u81f3\u9884\u6e29\u7684 T25 \u74f6\uff0c\u51cf\u5c11\u6e29\u5ea6\u523a\u6fc0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 48 \u5c0f\u65f6\u8d34\u58c1\u9a8c\u8bc1\uff0c\u786e\u4fdd\u8d34\u58c1\u7279\u6027\u7a33\u5b9a\uff0c\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff1b\u82e5\u9700\u540c\u6b65\u5f00\u5c55\u4e0e T24 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data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff0c\u4f7f\u7528\u9700\u7b26\u5408\u300a\u4f53\u5916\u57f9\u517b\u52a8\u7269\u7ec6\u80de\u7cfb\u7ba1\u7406\u89c4\u8303\u300b\u300a\u533b\u5b66\u79d1\u7814\u4f26\u7406\u5ba1\u67e5\u529e\u6cd5\u300b\u7b49\u6cd5\u89c4\uff1b\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf\u9ad8\u538b\u706d\u83cc\uff08121\u2103\uff0c30 \u5206\u949f\uff09\u5904\u7406\uff0c\u907f\u514d\u751f\u7269\u5371\u5bb3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460\u8d34\u58c1\u671f\u7ba1\u7406\uff1a\u4f20\u4ee3\u540e 48 \u5c0f\u65f6\u5185\u4e25\u683c\u7981\u6b62\u79fb\u52a8\u57f9\u517b\u74f6\uff0c\u5305\u62ec\u663e\u5fae\u955c\u89c2\u5bdf\u4ec5\u53ef\u8fdb\u884c 1 \u6b21\uff08\u8f7b\u7f13\u64cd\u4f5c\uff09\uff1b\u82e5\u53d1\u73b0\u90e8\u5206\u7ec6\u80de\u672a\u8d34\u58c1\uff0c\u65e0\u9700\u79bb\u5fc3\u91cd\u94fa\uff0c\u7ee7\u7eed\u57f9\u517b\u81f3 72 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