{"id":1594,"date":"2025-10-06T16:12:53","date_gmt":"2025-10-06T08:12:53","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=1594"},"modified":"2025-10-06T16:12:53","modified_gmt":"2025-10-06T08:12:53","slug":"12z%e4%ba%ba%e5%ad%90%e5%ae%ab%e5%86%85%e8%86%9c%e5%bc%82%e4%bd%8d%e7%bb%86%e8%83%9e","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/12z%e4%ba%ba%e5%ad%90%e5%ae%ab%e5%86%85%e8%86%9c%e5%bc%82%e4%bd%8d%e7%bb%86%e8%83%9e\/","title":{"rendered":"12Z\u4eba\u5b50\u5bab\u5185\u819c\u5f02\u4f4d\u7ec6\u80de"},"content":{"rendered":"<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u540d\u79f0\uff1a12Z \u4eba\u5b50\u5bab\u5185\u819c\u5f02\u4f4d\u7ec6\u80de<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u522b\u79f0\uff1a12z; 12-Z; Z12; Z-12; Z12 Eo; EEC12Z<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec4\u7ec7\u6765\u6e90\uff1a\u5b50\u5bab\uff08\u6e90\u4e8e\u5b50\u5bab\u5185\u819c\u5f02\u4f4d\u7ec4\u7ec7\uff0c\u5177\u5907\u5b50\u5bab\u5185\u819c\u5f02\u4f4d\u7ec6\u80de\u7684\u751f\u7269\u5b66\u7279\u6027\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u5f62\u6001\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u5355\u5c42\u8d34\u58c1\u751f\u957f\uff0c\u7ec6\u80de\u5448\u591a\u8fb9\u5f62\uff0c\u6392\u5217\u7d27\u5bc6\uff0c\u7b26\u5408\u4e0a\u76ae\u7ec6\u80de\u5178\u578b\u5f62\u6001\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u57f9\u517b\u57fa\uff1aDMEM\/F-12 \u57f9\u517b\u57fa + 10% FBS+ PS\uff08\u5e38\u89c4\u590d\u5408\u57fa\u7840\u57f9\u517b\u57fa\uff0c\u9700\u6309\u914d\u65b9\u7cbe\u51c6\u914d\u5236\uff0c\u786e\u4fdd\u8425\u517b\u5747\u8861\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8 95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c\u6e29\u5ea6 37\u2103\uff08\u5e38\u89c4\u4eba\u4f53\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09 \u987a\u4e30\u5feb\u9012<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528<\/p><\/div>\n<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u6838\u5fc3\u64cd\u4f5c<\/h1>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u4f20\u4ee3\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u5f53\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%-80%\uff08\u4e0a\u76ae\u7ec6\u80de\u5355\u5c42\u94fa\u6ee1\u74f6\u58c1\uff0c\u65e0\u660e\u663e\u5806\u53e0\uff0c\u591a\u8fb9\u5f62\u5f62\u6001\u5b8c\u6574\uff09\u65f6\uff0c\u5f03\u53bb\u65e7\u57f9\u517b\u57fa\uff0c\u7528\u65e0\u83cc PBS \u8f7b\u67d4\u51b2\u6d17\u7ec6\u80de\u8868\u9762 1 \u6b21\uff0c\u907f\u514d\u635f\u4f24\u7d27\u5bc6\u6392\u5217\u7684\u7ec6\u80de\u5c42\u3002<\/li>\n<li>\u52a0\u5165\u80f0\u9176\u6e29\u548c\u6d88\u5316\uff0c\u5f85\u7ec6\u80de\u8fb9\u7f18\u6536\u7f29\u3001\u95f4\u9699\u589e\u5927\uff08\u7ea6 2-3 \u5206\u949f\uff0c\u9700\u5bc6\u5207\u89c2\u5bdf\uff0c\u9632\u6b62\u8fc7\u5ea6\u6d88\u5316\u5bfc\u81f4\u7ec6\u80de\u7834\u788e\uff09\u540e\uff0c\u7acb\u5373\u52a0\u5165\u542b 10% FBS \u7684 DMEM\/F-12 \u57f9\u517b\u57fa\u7ec8\u6b62\u6d88\u5316\uff08\u8840\u6e05\u53ef\u5feb\u901f\u4e2d\u548c\u80f0\u9176\u6d3b\u6027\uff09\u3002<\/li>\n<li>\u7528\u79fb\u6db2\u5668\u8f7b\u67d4\u5439\u6253\u7ec6\u80de\u81f3\u5355\u7ec6\u80de\u60ac\u6db2\uff0c\u6309 1:3-1:5 \u6bd4\u4f8b\u63a5\u79cd\u5230\u65b0\u57f9\u517b\u74f6\uff0c\u5168\u7a0b\u52a8\u4f5c\u7f13\u6162\uff0c\u7ef4\u6301\u5b50\u5bab\u5185\u819c\u5f02\u4f4d\u7ec6\u80de\u7684\u4e0a\u76ae\u5f62\u6001\u4e0e\u589e\u6b96\u7279\u6027\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u6362\u6db2\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u6bcf 2-3 \u5929\u66f4\u6362 1 \u6b21\u65b0\u9c9c\u57f9\u517b\u57fa\uff0c\u6362\u6db2\u65f6\u5148\u7f13\u6162\u503e\u5012\u65e7\u57f9\u517b\u57fa\uff08\u907f\u514d\u76f4\u63a5\u51b2\u51fb\u7ec6\u80de\u5c42\u5bfc\u81f4\u7ec6\u80de\u8131\u843d\uff09\uff0c\u518d\u7528 PBS \u51b2\u6d17 1 \u6b21\uff0c\u6700\u540e\u52a0\u5165\u8db3\u91cf \u201cDMEM\/F-12+10% FBS+PS\u201d \u57f9\u517b\u57fa\uff0c\u786e\u4fdd\u8425\u517b\u6ee1\u8db3\u7ec6\u80de\u9700\u6c42\u3002<\/li>\n<li>\u82e5\u53d1\u73b0\u57f9\u517b\u57fa\u8f83\u5feb\u53d8\u9ec4\uff08\u4ee3\u8c22\u65fa\u76db\uff09\uff0c\u53ef\u9002\u5f53\u7f29\u77ed\u6362\u6db2\u95f4\u9694\uff0c\u907f\u514d\u4ee3\u8c22\u5e9f\u7269\u5806\u79ef\u6291\u5236\u7ec6\u80de\u6d3b\u6027\uff0c\u5f71\u54cd\u5b9e\u9a8c\u7ed3\u679c\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">3. \u51bb\u5b58\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u9009\u53d6\u5bf9\u6570\u671f\uff08\u589e\u6b96\u65fa\u76db\u3001\u4e0a\u76ae\u5f62\u6001\u5747\u4e00\u3001\u65e0\u6742\u7ec6\u80de\uff09\u7684\u7ec6\u80de\uff0c\u80f0\u9176\u6d88\u5316\u540e 1000rpm \u79bb\u5fc3 5 \u5206\u949f\uff0c\u5f03\u4e0a\u6e05\u6536\u96c6\u7ec6\u80de\u6c89\u6dc0\u3002<\/li>\n<li>\u7528\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\u7ec6\u80de\u81f3 1\u00d710\u2076-2\u00d710\u2076cells\/ml\uff0c\u5206\u88c5\u5230\u51bb\u5b58\u7ba1\u540e\uff0c\u6309 \u201c4\u2103\u9759\u7f6e 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u8f6c\u5165\u6db2\u6c2e\u201d \u7684\u68af\u5ea6\u964d\u6e29\uff0c\u51cf\u5c11\u4f4e\u6e29\u5bf9\u7ec6\u80de\u7684\u635f\u4f24\uff0c\u4fdd\u7559\u5176\u751f\u7269\u5b66\u7279\u6027\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">4. \u590d\u82cf\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u4ece\u6db2\u6c2e\u4e2d\u53d6\u51fa\u51bb\u5b58\u7ba1\uff0c\u7acb\u5373\u653e\u5165 37\u2103\u6c34\u6d74\u901f\u878d\uff081-2 \u5206\u949f\u5185\u5b8c\u5168\u878d\u5316\uff0c\u907f\u514d\u51b0\u6676\u53cd\u590d\u51bb\u878d\u7834\u574f\u7ec6\u80de\uff09\uff0c\u8fc5\u901f\u5c06\u7ec6\u80de\u60ac\u6db2\u8f6c\u79fb\u81f3\u79bb\u5fc3\u7ba1\u3002<\/li>\n<li>\u52a0\u5165 5 \u500d\u4f53\u79ef\u65b0\u9c9c \u201cDMEM\/F-12+10% FBS+PS\u201d \u57f9\u517b\u57fa\u7a00\u91ca\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u5f03\u4e0a\u6e05\uff0c\u7528\u9884\u70ed\u81f3 37\u2103\u7684\u57f9\u517b\u57fa\u91cd\u60ac\u7ec6\u80de\u540e\u63a5\u79cd\uff0c24 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\uff0c48 \u5c0f\u65f6\u540e\u6309\u5e38\u89c4\u6362\u6db2\u3002<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1a12Z \u4eba\u5b50\u5bab\u5185\u819c\u5f02\u4f4d\u7ec6\u80de \u7ec6\u80de\u522b\u79f0\uff1a12z<\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/12z%e4%ba%ba%e5%ad%90%e5%ae%ab%e5%86%85%e8%86%9c%e5%bc%82%e4%bd%8d%e7%bb%86%e8%83%9e\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        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