{"id":1602,"date":"2025-10-07T11:35:59","date_gmt":"2025-10-07T03:35:59","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=1602"},"modified":"2025-10-07T11:35:59","modified_gmt":"2025-10-07T03:35:59","slug":"pdac-x3%e4%ba%ba%e8%83%b0%e8%85%ba%e5%af%bc%e7%ae%a1%e7%99%8c%e7%bb%86%e8%83%9e","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/pdac-x3%e4%ba%ba%e8%83%b0%e8%85%ba%e5%af%bc%e7%ae%a1%e7%99%8c%e7%bb%86%e8%83%9e\/","title":{"rendered":"PDAC-X3\u4eba\u80f0\u817a\u5bfc\u7ba1\u764c\u7ec6\u80de"},"content":{"rendered":"<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u540d\u79f0\uff1aPDAC-X3 \u4eba\u80f0\u817a\u5bfc\u7ba1\u764c\u7ec6\u80de<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec4\u7ec7\u6765\u6e90\uff1a\u80f0\u817a\uff08\u6e90\u81ea 73 \u5c81\u65e0\u5438\u70df\u996e\u9152\u3001\u65e0\u4e59\u809d\u4e19\u809d\u611f\u67d3\u53f2\u7537\u6027\u80f0\u817a\u764c\u60a3\u8005\uff0c\u5177\u5907\u80f0\u817a\u5bfc\u7ba1\u764c\u7ec6\u80de\u7279\u5f81\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f\uff0c<strong>\u5b8c\u5168\u4e27\u5931\u63a5\u89e6\u6291\u5236\u80fd\u529b<\/strong>\uff08\u9700\u4e25\u683c\u63a7\u5236\u5bc6\u5ea6\uff0c\u907f\u514d\u8fc7\u5ea6\u589e\u6b96\u5bfc\u81f4\u7ec6\u80de\u5806\u79ef\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u5f62\u6001\uff1a\u4e3b\u8981\u5448\u591a\u8fb9\u5f62\uff0c\u542b\u5927\u800c\u660e\u663e\u7684\u7ec6\u80de\u6838\u53ca\u6e05\u6670\u6838\u4ec1\uff1b\u53ef\u89c1\u591a\u6838\u7ec6\u80de\u4e0e\u5de8\u6838\u7ec6\u80de<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u80cc\u666f\u8865\u5145\uff1a<\/p><\/div>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u589e\u6b96\u7279\u6027\uff1a\u7fa4\u4f53\u500d\u589e\u65f6\u95f4 50 \u5c0f\u65f6\uff0c\u514d\u75ab\u7ec4\u5316\u663e\u793a CK7\u3001CK19\uff08\u4e0a\u76ae\u6807\u5fd7\u7269\uff09\u3001E &#8211; \u9499\u9ecf\u86cb\u767d\u3001\u6ce2\u5f62\u86cb\u767d\u3001CA19-9 \u9633\u6027\uff0cKi-67 \u8868\u8fbe\u7387 30%\uff08\u63d0\u793a\u4e2d\u7b49\u589e\u6b96\u6d3b\u6027\uff09\uff0c\u65e0 CEA \u8868\u8fbe<\/li>\n<li>\u529f\u80fd\u7279\u6027\uff1a\u60ac\u6d6e\u57f9\u517b\u53ef\u5f62\u6210\u7403\u72b6\u3001\u56ca\u72b6\u53ca\u5b62\u5b50\u72b6 \/ \u5206\u679d\u72b6\u7c7b\u5668\u5b98\uff0c\u5177\u5907\u590d\u6742\u817a\u4f53\u6837\u7ed3\u6784\u5f62\u6210\u80fd\u529b<\/li>\n<li>\u81f4\u7624\u6027\uff1aBALB\/C \u88f8\u9f20\u76ae\u4e0b\u63a5\u79cd\u6210\u7624\u7387 33%\uff0c\u80bf\u7624\u751f\u957f\u8fc5\u901f\uff084 \u5468\u76f4\u5f84\u8fbe 0.7cm\uff09\uff0c\u809d\u80ba\u65e0\u8f6c\u79fb\u7076<\/li>\n<li>\u6838\u578b\u7279\u5f81\uff1a\u590d\u6742\u6838\u578b\uff0c75% \u4e3a\u4e9a\u4e09\u500d\u4f53\uff0c25% \u4e3a\u4e9a\u56db\u500d\u4f53\uff0c\u5178\u578b\u6838\u578b\u4e3a 53\u3001XY der (1) inv (9) der (22)<\/li>\n<li>\u836f\u7269\u654f\u611f\u6027\uff1a\u5bf9\u5965\u6c99\u5229\u94c2\u8010\u836f\uff08IC50=18.86\u00b5mol\/L\uff09\uff0c\u5bf9 5 &#8211; \u6c1f\u5c3f\u5627\u5576\uff08IC50=7.109\u00b5mol\/L\uff09\u3001\u5409\u897f\u4ed6\u6ee8\uff08IC50=0.029\u00b5mol\/L\uff09\u3001\u7d2b\u6749\u9187\uff08IC50=0.0046\u00b5g\/mL\uff09\u654f\u611f\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u9634\u6027<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u57f9\u517b\u57fa\uff1a1640 \u57f9\u517b\u57fa + 10% FBS+PS\uff08\u4e25\u683c\u6309\u914d\u65b9\u914d\u5236\uff0c\u4fdd\u969c\u7ec6\u80de\u589e\u6b96\u3001\u7c7b\u5668\u5b98\u5f62\u6210\u53ca\u836f\u7269\u654f\u611f\u6027\u7279\u6027\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8 95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c\u6e29\u5ea6 37\u2103\uff08\u5e38\u89c4\u4eba\u4f53\u764c\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09 \u987a\u4e30\u5feb\u9012<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528<\/li>\n<\/ul>\n<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u6838\u5fc3\u64cd\u4f5c<\/h1>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u4f20\u4ee3\u64cd\u4f5c\uff08\u91cd\u70b9\u5173\u6ce8\u63a5\u89e6\u6291\u5236\u4e27\u5931\u7279\u6027\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u5f53\u7ec6\u80de\u5bc6\u5ea6\u8fbe 60%-70% \u65f6\u7acb\u5373\u4f20\u4ee3\uff08\u56e0\u5b8c\u5168\u65e0\u63a5\u89e6\u6291\u5236\uff0c\u5bc6\u5ea6\u8d85 80% \u6613\u51fa\u73b0\u5bc6\u96c6\u5806\u53e0\uff0c\u5f71\u54cd\u7ec6\u80de\u6d3b\u6027\uff09\uff0c\u5f03\u53bb\u65e7\u57f9\u517b\u57fa\uff0c\u7528\u65e0\u83cc PBS \u8f7b\u67d4\u51b2\u6d17\u7ec6\u80de\u8868\u9762 1 \u6b21\uff0c\u907f\u514d\u635f\u4f24\u591a\u6838 \/ \u5de8\u6838\u7ec6\u80de\u3002<\/li>\n<li>\u52a0\u5165\u80f0\u9176\u6e29\u548c\u6d88\u5316\uff0c\u5f85\u7ec6\u80de\u8fb9\u7f18\u6536\u7f29\u3001\u591a\u8fb9\u5f62\u5f62\u6001\u7565\u53d8\u5706\uff08\u7ea6 2-3 \u5206\u949f\uff0c\u56e0\u7ec6\u80de\u589e\u6b96\u5feb\uff0c\u6d88\u5316\u901f\u5ea6\u8f83\u5176\u4ed6 PDAC \u4e9a\u578b\u7565\u5feb\uff0c\u9700\u5bc6\u5207\u89c2\u5bdf\uff09\u540e\uff0c\u7acb\u5373\u52a0\u5165\u542b 10% FBS \u7684 1640 \u57f9\u517b\u57fa\u7ec8\u6b62\u6d88\u5316\u3002<\/li>\n<li>\u7528\u79fb\u6db2\u5668\u8f7b\u67d4\u5439\u6253\u7ec6\u80de\u81f3\u5355\u7ec6\u80de\u60ac\u6db2\uff08\u52a8\u4f5c\u7f13\u6162\uff0c\u51cf\u5c11\u5de8\u6838\u7ec6\u80de\u7834\u788e\uff09\uff0c\u6309 1:4-1:5 \u6bd4\u4f8b\u63a5\u79cd\u5230\u65b0\u57f9\u517b\u74f6\uff08\u6269\u5927\u4f20\u4ee3\u6bd4\u4f8b\uff0c\u63a7\u5236\u540e\u7eed\u589e\u6b96\u901f\u5ea6\uff09\uff0c\u7ef4\u6301\u5176\u7c7b\u5668\u5b98\u5f62\u6210\u80fd\u529b\u4e0e\u836f\u7269\u654f\u611f\u6027\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u6362\u6db2\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u6bcf 1-2 \u5929\u66f4\u6362 1 \u6b21\u65b0\u9c9c\u57f9\u517b\u57fa\uff08\u56e0\u500d\u589e\u65f6\u95f4\u77ed\u3001\u4ee3\u8c22\u65fa\u76db\uff0c\u9700\u7f29\u77ed\u6362\u6db2\u95f4\u9694\uff09\uff0c\u6362\u6db2\u65f6\u5148\u7f13\u6162\u503e\u5012\u65e7\u57f9\u517b\u57fa\uff08\u907f\u514d\u76f4\u63a5\u51b2\u51fb\u7ec6\u80de\u5c42\u5bfc\u81f4\u591a\u6838\u7ec6\u80de\u8131\u843d\uff09\uff0c\u518d\u7528 PBS \u51b2\u6d17 1 \u6b21\uff0c\u6700\u540e\u52a0\u5165\u8db3\u91cf \u201c1640+10% FBS+PS\u201d \u57f9\u517b\u57fa\u3002<\/li>\n<li>\u82e5\u5f00\u5c55\u836f\u7269\u654f\u611f\u6027\u5b9e\u9a8c\uff0c\u6362\u6db2\u540e\u9700\u7a33\u5b9a\u57f9\u517b 24 \u5c0f\u65f6\u518d\u52a0\u836f\uff0c\u907f\u514d\u6362\u6db2\u5e94\u6fc0\u5f71\u54cd\u5b9e\u9a8c\u7ed3\u679c\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">3. \u7c7b\u5668\u5b98\u57f9\u517b\u64cd\u4f5c\uff08\u4e13\u9879\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u6536\u96c6\u5bf9\u6570\u671f\u7ec6\u80de\uff0c\u7528\u65e0\u83cc PBS \u6d17\u6da4 2 \u6b21\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u540e\u5f03\u4e0a\u6e05\uff0c\u7528\u7c7b\u5668\u5b98\u5b8c\u5168\u57f9\u517b\u57fa\u91cd\u60ac\u7ec6\u80de\u81f3 8\u00d710\u2074cells\/mL\uff08\u5bc6\u5ea6\u4f4e\u4e8e PDAC-X1\/X2\uff0c\u9002\u914d\u5176\u5feb\u901f\u589e\u6b96\u7279\u6027\uff09\u3002<\/li>\n<li>\u5c06\u7ec6\u80de\u60ac\u6db2\u63a5\u79cd\u5230\u8d85\u4f4e\u9644\u7740\u57f9\u517b\u677f\uff0c\u6bcf\u5b54 1mL\uff0c\u7f6e\u4e8e\u57f9\u517b\u7bb1\u9759\u7f6e\u57f9\u517b\uff1b\u57f9\u517b\u7b2c 2 \u5929\u8865\u52a0 500\u03bcL \u65b0\u9c9c\u7c7b\u5668\u5b98\u57f9\u517b\u57fa\uff0c\u7b2c 5-6 \u5929\u53ef\u89c2\u5bdf\u5230\u5b62\u5b50\u72b6 \/ \u5206\u679d\u72b6\u7c7b\u5668\u5b98\uff0c\u6309\u9700\u8fdb\u884c\u540e\u7eed\u5b9e\u9a8c\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">4. \u51bb\u5b58\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u9009\u53d6\u5bf9\u6570\u671f\uff08\u589e\u6b96\u65fa\u76db\u3001\u591a\u8fb9\u5f62\u5f62\u6001\u5747\u4e00\u3001\u591a\u6838 \/ \u5de8\u6838\u7ec6\u80de\u6bd4\u4f8b\u6b63\u5e38\uff0c\u5bc6\u5ea6\u672a\u8d85 70%\uff09\u7684\u7ec6\u80de\uff0c\u80f0\u9176\u6d88\u5316\u540e 1000rpm \u79bb\u5fc3 5 \u5206\u949f\uff0c\u5f03\u4e0a\u6e05\u6536\u96c6\u7ec6\u80de\u6c89\u6dc0\u3002<\/li>\n<li>\u7528\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\u7ec6\u80de\u81f3 1\u00d710\u2076-2\u00d710\u2076cells\/ml\uff0c\u5206\u88c5\u540e\u6309 \u201c4\u2103\u9759\u7f6e 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u8f6c\u5165\u6db2\u6c2e\u201d \u7684\u68af\u5ea6\u964d\u6e29\uff0c\u51cf\u5c11\u4f4e\u6e29\u5bf9\u590d\u6742\u6838\u578b\u7ec6\u80de\u7684\u635f\u4f24\uff0c\u4fdd\u7559\u7c7b\u5668\u5b98\u5f62\u6210\u80fd\u529b\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">5. \u590d\u82cf\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u4ece\u6db2\u6c2e\u4e2d\u53d6\u51fa\u51bb\u5b58\u7ba1\uff0c\u7acb\u5373\u653e\u5165 37\u2103\u6c34\u6d74\u901f\u878d\uff081 \u5206\u949f\u5185\u5b8c\u5168\u878d\u5316\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u7834\u574f\u7ec6\u80de\uff09\uff0c\u8fc5\u901f\u5c06\u7ec6\u80de\u60ac\u6db2\u8f6c\u79fb\u81f3\u79bb\u5fc3\u7ba1\u3002<\/li>\n<li>\u52a0\u5165 5 \u500d\u4f53\u79ef\u65b0\u9c9c \u201c1640+10% FBS+PS\u201d 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