{"id":1607,"date":"2025-10-07T23:36:59","date_gmt":"2025-10-07T15:36:59","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=1607"},"modified":"2025-10-07T23:36:59","modified_gmt":"2025-10-07T15:36:59","slug":"gbc-x1%e4%ba%ba%e8%83%86%e5%9b%8a%e7%99%8c%e7%bb%86%e8%83%9e","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/gbc-x1%e4%ba%ba%e8%83%86%e5%9b%8a%e7%99%8c%e7%bb%86%e8%83%9e\/","title":{"rendered":"GBC-X1\u4eba\u80c6\u56ca\u764c\u7ec6\u80de"},"content":{"rendered":"<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u540d\u79f0\uff1aGBC-X1 \u4eba\u80c6\u56ca\u764c\u7ec6\u80de<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec4\u7ec7\u6765\u6e90\uff1a\u80c6\u56ca\uff08\u6e90\u81ea 72 \u5c81\u65e0\u5438\u70df\u996e\u9152\u53f2\u3001\u6709\u4e59\u809d\u611f\u67d3\u53f2\u5973\u6027\u80c6\u56ca\u764c\u60a3\u8005\uff0cSTR \u8bc1\u5b9e\u4e0e\u539f\u53d1\u80bf\u7624\u9ad8\u5ea6\u4e00\u81f4\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f\uff0c<strong>\u63a5\u89e6\u6291\u5236\u6d88\u5931<\/strong>\uff08\u9700\u4e25\u683c\u63a7\u5236\u5bc6\u5ea6\uff0c\u907f\u514d\u8fc7\u5ea6\u589e\u6b96\u5806\u79ef\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u5f62\u6001\uff1a\u5178\u578b\u4e0a\u76ae\u80bf\u7624\u7279\u5f81\uff0c\u591a\u5448\u591a\u8fb9\u5f62\uff0c\u6838\u4ec1\u663e\u8457\uff0c\u53ef\u89c1\u591a\u6838\u7ec6\u80de\u4e0e\u5de8\u6838\u7ec6\u80de<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u80cc\u666f\u8865\u5145\uff1a<\/p><\/div>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u589e\u6b96\u7279\u6027\uff1a\u79cd\u7fa4\u500d\u589e\u65f6\u95f4 32 \u5c0f\u65f6\uff08\u589e\u6b96\u901f\u5ea6\u5feb\uff09\uff1b\u514d\u75ab\u7ec4\u5316\u663e\u793a CK7\u3001CK19\uff08\u4e0a\u76ae\u6807\u5fd7\u7269\uff09\u3001E &#8211; \u9499\u9ecf\u86cb\u767d\u9633\u6027\uff0cVimentin \u5f31\u9633\u6027\uff0cCEA \u7076\u6027\u8868\u8fbe\uff0cKi-67 \u8868\u8fbe\u7387 35%\uff08\u589e\u6b96\u6d3b\u6027\u8f83\u5f3a\uff09<\/li>\n<li>\u529f\u80fd\u7279\u6027\uff1a\u60ac\u6d6e\u57f9\u517b\u53ef\u9ad8\u6548\u5f62\u6210\u80bf\u7624\u7403\u4f53\uff08\u968f\u57f9\u517b\u65f6\u95f4\u589e\u5927\uff09\uff1bTranswell \u5b9e\u9a8c\u663e\u793a 24 \u5c0f\u65f6\u8fc1\u79fb\u7ec6\u80de\u6570 9.33\u00b10.47\u3001\u4fb5\u88ad\u7ec6\u80de\u6570 3.33\u00b10.47\uff0c48 \u5c0f\u65f6\u5206\u522b\u8fbe 195.67\u00b12.49\u3001186\u00b14.90<\/li>\n<li>\u81f4\u7624\u6027\uff1aNXG \u5c0f\u9f20\u76ae\u4e0b\u63a5\u79cd\u6210\u7624\u7387 80%\uff0c\u809d\u80ba\u65e0\u8f6c\u79fb\u7076<\/li>\n<li>\u6838\u578b\u7279\u5f81\uff1a\u4e9a\u56db\u500d\u4f53\u5f02\u5e38\u6838\u578b\uff0c\u67d3\u8272\u4f53\u6570\u76ee\u4e0e\u5f62\u6001\u5dee\u5f02\u663e\u8457\uff0c\u5178\u578b\u6838\u578b\u4e3a 98\uff0cXXXX del (4) p (12) del (5) p (21) der (10)<\/li>\n<li>\u836f\u7269\u654f\u611f\u6027\uff1a\u5bf9\u5965\u6c99\u5229\u94c2\u8010\u836f\uff08IC50=10.65\u00b5mol\/L\uff09\uff0c\u5bf9 5 &#8211; \u6c1f\u5c3f\u5627\u5576\uff08IC50=0.50\u00b5mol\/L\uff09\u3001\u5409\u897f\u4ed6\u6ee8\uff08IC50=0.0035\u00b5mol\/L\uff09\u3001\u7d2b\u6749\u9187\uff08IC50=0.55\u00b5g\/mL\uff09\u654f\u611f\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u9634\u6027<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u57f9\u517b\u57fa\uff1a1640 \u57f9\u517b\u57fa + 10% FBS+PS\uff08\u4e25\u683c\u6309\u914d\u65b9\u914d\u5236\uff0c\u4fdd\u969c\u7ec6\u80de\u589e\u6b96\u3001\u80bf\u7624\u7403\u5f62\u6210\u53ca\u836f\u7269\u654f\u611f\u6027\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8 95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c\u6e29\u5ea6 37\u2103\uff08\u5e38\u89c4\u4eba\u4f53\u764c\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09 \u987a\u4e30\u5feb\u9012<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528<\/li>\n<\/ul>\n<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u6838\u5fc3\u64cd\u4f5c<\/h1>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u4f20\u4ee3\u64cd\u4f5c\uff08\u91cd\u70b9\u5e94\u5bf9\u5feb\u589e\u6b96\u4e0e\u65e0\u63a5\u89e6\u6291\u5236\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u5f53\u7ec6\u80de\u5bc6\u5ea6\u8fbe 50%-60% \u65f6\u7acb\u5373\u4f20\u4ee3\uff08\u500d\u589e\u5feb\u4e14\u65e0\u63a5\u89e6\u6291\u5236\uff0c\u5bc6\u5ea6\u8d85 70% \u6613\u5bc6\u96c6\u5806\u53e0\uff09\uff0c\u5f03\u53bb\u65e7\u57f9\u517b\u57fa\uff0c\u7528\u65e0\u83cc PBS \u8f7b\u67d4\u51b2\u6d17\u7ec6\u80de\u8868\u9762 1 \u6b21\uff0c\u907f\u514d\u635f\u4f24\u591a\u6838 \/ \u5de8\u6838\u7ec6\u80de\u3002<\/li>\n<li>\u52a0\u5165\u80f0\u9176\u6e29\u548c\u6d88\u5316\uff0c\u5f85\u7ec6\u80de\u8fb9\u7f18\u6536\u7f29\u3001\u591a\u8fb9\u5f62\u7565\u53d8\u5706\uff08\u7ea6 1.5-2.5 \u5206\u949f\uff0c\u589e\u6b96\u5feb\u5bfc\u81f4\u6d88\u5316\u901f\u5ea6\u5feb\uff0c\u9700\u9ad8\u9891\u89c2\u5bdf\uff09\u540e\uff0c\u7acb\u5373\u52a0\u5165\u542b 10% FBS \u7684 1640 \u57f9\u517b\u57fa\u7ec8\u6b62\u6d88\u5316\u3002<\/li>\n<li>\u7528\u79fb\u6db2\u5668\u8f7b\u67d4\u5439\u6253\u7ec6\u80de\u81f3\u5355\u7ec6\u80de\u60ac\u6db2\uff08\u52a8\u4f5c\u7f13\u6162\uff0c\u51cf\u5c11\u5de8\u6838\u7ec6\u80de\u7834\u788e\uff09\uff0c\u6309 1:5-1:6 \u6bd4\u4f8b\u63a5\u79cd\u5230\u65b0\u57f9\u517b\u74f6\uff08\u6269\u5927\u4f20\u4ee3\u6bd4\u4f8b\u63a7\u5236\u589e\u6b96\u901f\u5ea6\uff09\uff0c\u7ef4\u6301\u5176\u8fc1\u79fb\u4fb5\u88ad\u80fd\u529b\u4e0e\u836f\u7269\u654f\u611f\u6027\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u6362\u6db2\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u6bcf 1 \u5929\u66f4\u6362 1 \u6b21\u65b0\u9c9c\u57f9\u517b\u57fa\uff08\u4ee3\u8c22\u65fa\u76db\uff0c\u9700\u53ca\u65f6\u8865\u5145\u8425\u517b\uff09\uff0c\u6362\u6db2\u65f6\u5148\u7f13\u6162\u503e\u5012\u65e7\u57f9\u517b\u57fa\uff08\u907f\u514d\u76f4\u63a5\u51b2\u51fb\u7ec6\u80de\u5c42\u5bfc\u81f4\u8131\u843d\uff09\uff0c\u518d\u7528 PBS \u51b2\u6d17 1 \u6b21\uff0c\u6700\u540e\u52a0\u5165\u8db3\u91cf \u201c1640+10% FBS+PS\u201d \u57f9\u517b\u57fa\u3002<\/li>\n<li>\u82e5\u5f00\u5c55\u836f\u7269\u654f\u611f\u6027\u5b9e\u9a8c\uff08\u5982\u5409\u897f\u4ed6\u6ee8\u836f\u6548\u68c0\u6d4b\uff09\uff0c\u6362\u6db2\u540e\u9700\u7a33\u5b9a\u57f9\u517b 24 \u5c0f\u65f6\u518d\u52a0\u836f\uff0c\u907f\u514d\u6362\u6db2\u5e94\u6fc0\u5e72\u6270\u5b9e\u9a8c\u7ed3\u679c\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">3. \u80bf\u7624\u7403\u57f9\u517b\u4e0e Transwell \u5b9e\u9a8c\u64cd\u4f5c\uff08\u4e13\u9879\uff09<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\uff081\uff09\u80bf\u7624\u7403\u57f9\u517b<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u6536\u96c6\u5bf9\u6570\u671f\u7ec6\u80de\uff0c\u7528\u65e0\u8840\u6e05\u57f9\u517b\u57fa\u6d17\u6da4 2 \u6b21\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u540e\u5f03\u4e0a\u6e05\uff0c\u7528\u65e0\u8840\u6e05\u80bf\u7624\u7403\u57f9\u517b\u57fa\u91cd\u60ac\u7ec6\u80de\u81f3 7\u00d710\u2074cells\/mL\u3002<\/li>\n<li>\u63a5\u79cd\u5230\u8d85\u4f4e\u9644\u7740\u57f9\u517b\u677f\uff0c\u6bcf\u5b54 1mL\uff0c\u9759\u7f6e\u57f9\u517b\uff1b\u57f9\u517b\u7b2c 2 \u5929\u8865\u52a0 500\u03bcL \u65e0\u8840\u6e05\u57f9\u517b\u57fa\uff0c\u7b2c 5-6 \u5929\u53ef\u89c2\u5bdf\u5230\u660e\u663e\u589e\u5927\u7684\u80bf\u7624\u7403\u4f53\u3002<\/li>\n<\/ul>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\uff082\uff09Transwell \u8fc1\u79fb \/ \u4fb5\u88ad\u5b9e\u9a8c<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u8fc1\u79fb\u5b9e\u9a8c\uff1a\u6536\u96c6\u5bf9\u6570\u671f\u7ec6\u80de\uff0c\u7528\u65e0\u8840\u6e05 1640 \u57f9\u517b\u57fa\u91cd\u60ac\u81f3 5\u00d710\u2075cells\/mL\uff0c\u4e0a\u5ba4\u52a0 200\u03bcL \u7ec6\u80de\u60ac\u6db2\uff0c\u4e0b\u5ba4\u52a0 600\u03bcL \u542b 10% FBS \u7684 1640 \u57f9\u517b\u57fa\uff0c\u57f9\u517b 24-48 \u5c0f\u65f6\u540e\u8ba1\u6570\u3002<\/li>\n<li>\u4fb5\u88ad\u5b9e\u9a8c\uff1a\u63d0\u524d\u7528 Matrigel\u2122\u5305\u88ab Transwell \u5c0f\u5ba4\uff0c\u5176\u4f59\u64cd\u4f5c\u540c\u8fc1\u79fb\u5b9e\u9a8c\uff0c\u786e\u4fdd\u7ec6\u80de\u4fb5\u88ad\u80fd\u529b\u68c0\u6d4b\u51c6\u786e\u6027\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">4. \u51bb\u5b58\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u9009\u53d6\u5bf9\u6570\u671f\uff08\u589e\u6b96\u65fa\u76db\u3001\u591a\u8fb9\u5f62\u5f62\u6001\u5360\u6bd4\u6b63\u5e38\u3001\u591a\u6838 \/ \u5de8\u6838\u7ec6\u80de\u6bd4\u4f8b\u7a33\u5b9a\u3001\u5bc6\u5ea6\u672a\u8d85 60%\uff09\u7684\u7ec6\u80de\uff0c\u80f0\u9176\u6d88\u5316\u540e 1000rpm \u79bb\u5fc3 5 \u5206\u949f\uff0c\u5f03\u4e0a\u6e05\u6536\u96c6\u7ec6\u80de\u6c89\u6dc0\u3002<\/li>\n<li>\u7528\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\u7ec6\u80de\u81f3 1\u00d710\u2076-2\u00d710\u2076cells\/ml\uff0c\u5206\u88c5\u540e\u6309 \u201c4\u2103\u9759\u7f6e 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u8f6c\u5165\u6db2\u6c2e\u201d \u7684\u68af\u5ea6\u964d\u6e29\uff0c\u51cf\u5c11\u4f4e\u6e29\u5bf9\u4e9a\u56db\u500d\u4f53\u7ec6\u80de\u7684\u635f\u4f24\uff0c\u4fdd\u7559\u80bf\u7624\u7403\u5f62\u6210\u80fd\u529b\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">5. \u590d\u82cf\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u4ece\u6db2\u6c2e\u4e2d\u53d6\u51fa\u51bb\u5b58\u7ba1\uff0c\u7acb\u5373\u653e\u5165 37\u2103\u6c34\u6d74\u901f\u878d\uff081 \u5206\u949f\u5185\u5b8c\u5168\u878d\u5316\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u7834\u574f\u7ec6\u80de\uff09\uff0c\u8fc5\u901f\u5c06\u7ec6\u80de\u60ac\u6db2\u8f6c\u79fb\u81f3\u79bb\u5fc3\u7ba1\u3002<\/li>\n<li>\u52a0\u5165 5 \u500d\u4f53\u79ef\u65b0\u9c9c \u201c1640+10% FBS+PS\u201d \u57f9\u517b\u57fa\u7a00\u91ca\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u5f03\u4e0a\u6e05\uff0c\u7528\u9884\u70ed\u81f3 37\u2103\u7684\u57f9\u517b\u57fa\u91cd\u60ac\u7ec6\u80de\u540e\u63a5\u79cd\uff1b\u590d\u82cf\u540e 12 \u5c0f\u65f6\u89c2\u5bdf\u8d34\u58c1\u60c5\u51b5\uff0c24 \u5c0f\u65f6\u540e\u6309\u5e38\u89c4\u6362\u6db2\uff0c\u56e0\u589e\u6b96\u5feb\u9700\u53ca\u65f6\u63a7\u5bc6\u5ea6\u3002<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aGBC-X1 \u4eba\u80c6\u56ca\u764c\u7ec6\u80de \u79cd\u5c5e\u6765\u6e90\uff1a\u4eba \u7ec4<\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/gbc-x1%e4%ba%ba%e8%83%86%e5%9b%8a%e7%99%8c%e7%bb%86%e8%83%9e\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        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