{"id":1615,"date":"2025-10-07T23:56:12","date_gmt":"2025-10-07T15:56:12","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=1615"},"modified":"2025-10-07T23:56:13","modified_gmt":"2025-10-07T15:56:13","slug":"hek293-fgfr1nluc","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/hek293-fgfr1nluc\/","title":{"rendered":"HEK293-FGFR1(Nluc)"},"content":{"rendered":"<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h1>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u540d\u79f0\uff1aHEK293-FGFR1 (Nluc) \u4eba\u80be\u4e0a\u76ae\u7ec6\u80de\u7a33\u8f6c\u682a<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec4\u7ec7\u6765\u6e90\uff1a\u80da\u80ce\u80be\uff08\u6e90\u4e8e\u4eba\u80da\u80ce\u80be\u810f\u7ec4\u7ec7\uff0c\u4e3a HEK293 \u7ec6\u80de\u884d\u751f\u7a33\u8f6c\u682a\uff0c\u643a\u5e26 FGFR1 \u57fa\u56e0\u4e0e Nluc \u8367\u5149\u7d20\u9176\u62a5\u544a\u57fa\u56e0\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u5f62\u6001\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u5355\u5c42\u8d34\u58c1\u751f\u957f\uff0c\u5448\u591a\u8fb9\u5f62\uff0c\u6392\u5217\u7d27\u5bc6\uff0c\u7b26\u5408\u80be\u4e0a\u76ae\u7ec6\u80de\u5178\u578b\u5f62\u6001\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u5173\u952e\u6ce8\u610f\uff1a<\/p><\/div>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u836f\u7269\u7b5b\u9009\uff1apuro\uff08\u560c\u5464\u9709\u7d20\uff09\u836f\u7b5b\u6d53\u5ea6 2.0\u03bcg\/ml\uff0c\u5e38\u89c4\u57f9\u517b\u5efa\u8bae\u7528 1.0\u03bcg\/ml puro \u7ef4\u6301\u7a33\u8f6c\u7279\u6027\uff1bbsd\uff08\u535a\u6765\u9709\u7d20\uff09\u836f\u7b5b\u6d53\u5ea6 8.0\u03bcg\/ml\uff0c\u5e38\u89c4\u57f9\u517b\u5efa\u8bae\u7528 4.0\u03bcg\/ml bsd \u8f85\u52a9\u7ef4\u6301\uff0c\u9632\u6b62\u975e\u7a33\u8f6c\u7ec6\u80de\u6c61\u67d3<br \/>\n\u57f9\u517b\u57fa\uff1a90% MEM \u57f9\u517b\u57fa + 10% FBS+PS\uff08\u9700\u989d\u5916\u6dfb\u52a0\u6307\u5b9a\u6d53\u5ea6 puro\/bsd\uff0c\u4fdd\u969c\u7a33\u8f6c\u682a\u7eaf\u5ea6\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8 95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c\u6e29\u5ea6 37\u2103\uff08\u5e38\u89c4\u4eba\u4f53\u4e0a\u76ae\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff08\u63a8\u8350 IMC-701\uff09\uff0c\u6db2\u6c2e\u50a8\u5b58<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09 \u987a\u4e30\u5feb\u9012<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528<\/li>\n<\/ul>\n<h1 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u57f9\u517b\u6838\u5fc3\u64cd\u4f5c<\/h1>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u4f20\u4ee3\u64cd\u4f5c\uff08\u91cd\u70b9\u7ef4\u6301\u7a33\u8f6c\u7279\u6027\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u5f53\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%-80%\uff08\u4e0a\u76ae\u7ec6\u80de\u5355\u5c42\u94fa\u6ee1\u74f6\u58c1\uff0c\u65e0\u5bc6\u96c6\u5806\u53e0\uff0c\u591a\u8fb9\u5f62\u5f62\u6001\u5b8c\u6574\uff09\u65f6\uff0c\u5f03\u53bb\u65e7\u57f9\u517b\u57fa\uff0c\u7528\u65e0\u83cc PBS \u8f7b\u67d4\u51b2\u6d17\u7ec6\u80de\u8868\u9762 1 \u6b21\uff0c\u907f\u514d\u635f\u4f24\u7d27\u5bc6\u6392\u5217\u7684\u7ec6\u80de\u5c42\u3002<\/li>\n<li>\u52a0\u5165\u80f0\u9176\u6e29\u548c\u6d88\u5316\uff0c\u5f85\u7ec6\u80de\u8fb9\u7f18\u6536\u7f29\u3001\u95f4\u9699\u589e\u5927\uff08\u7ea6 2-3 \u5206\u949f\uff0cHEK293 \u884d\u751f\u682a\u6d88\u5316\u654f\u611f\uff0c\u9700\u9010\u74f6\u89c2\u5bdf\uff0c\u9632\u6b62\u8fc7\u5ea6\u6d88\u5316\u5bfc\u81f4\u7ec6\u80de\u7834\u788e\uff09\u540e\uff0c\u7acb\u5373\u52a0\u5165\u542b 10% FBS \u7684 MEM \u57f9\u517b\u57fa\u7ec8\u6b62\u6d88\u5316\uff08\u8840\u6e05\u53ef\u5feb\u901f\u4e2d\u548c\u80f0\u9176\u6d3b\u6027\uff09\u3002<\/li>\n<li>\u7528\u79fb\u6db2\u5668\u8f7b\u67d4\u5439\u6253\u7ec6\u80de\u81f3\u5355\u7ec6\u80de\u60ac\u6db2\uff0c\u6309 1:3-1:5 \u6bd4\u4f8b\u63a5\u79cd\u5230\u65b0\u57f9\u517b\u74f6\uff0c\u63a5\u79cd\u540e\u9700\u7acb\u5373\u6dfb\u52a0 1.0\u03bcg\/ml puro \u4e0e 4.0\u03bcg\/ml bsd\uff0c\u6291\u5236\u975e\u7a33\u8f6c\u7ec6\u80de\u589e\u6b96\uff0c\u7ef4\u6301\u7a33\u8f6c\u682a\u7eaf\u5ea6\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u6362\u6db2\u64cd\u4f5c\uff08\u91cd\u70b9\u628a\u63a7\u836f\u7269\u6d53\u5ea6\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u6bcf 2-3 \u5929\u66f4\u6362 1 \u6b21\u65b0\u9c9c\u57f9\u517b\u57fa\uff0c\u9700\u4e25\u683c\u6309 \u201c90% MEM+10% FBS+PS+1.0\u03bcg\/ml puro+4.0\u03bcg\/ml bsd\u201d \u914d\u65b9\u914d\u5236\uff0c\u786e\u4fdd\u836f\u7269\u6d53\u5ea6\u7cbe\u51c6 \u2014\u2014 \u6d53\u5ea6\u8fc7\u9ad8\u6613\u5bfc\u81f4\u7ec6\u80de\u6d3b\u6027\u4e0b\u964d\uff0c\u8fc7\u4f4e\u5219\u53ef\u80fd\u4e22\u5931\u7a33\u8f6c\u7279\u6027\u3002<\/li>\n<li>\u503e\u5012\u65e7\u57f9\u517b\u57fa\u65f6\u52a8\u4f5c\u7f13\u6162\uff08\u907f\u514d\u76f4\u63a5\u51b2\u51fb\u7ec6\u80de\u5c42\u5bfc\u81f4\u7ec6\u80de\u8131\u843d\uff09\uff0c\u7528 PBS \u51b2\u6d17 1 \u6b21\u540e\u52a0\u5165\u8db3\u91cf\u65b0\u57f9\u517b\u57fa\uff1b\u82e5\u5f00\u5c55 FGF1 \u6fc0\u6d3b\u5b9e\u9a8c\uff0c\u5b9e\u9a8c\u524d 12 \u5c0f\u65f6\u9700\u66f4\u6362\u65e0\u836f\u57f9\u517b\u57fa\uff0c\u907f\u514d\u836f\u7269\u5e72\u6270\u57fa\u56e0\u8868\u8fbe\u6216\u86cb\u767d\u68c0\u6d4b\u7ed3\u679c\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">3. \u51bb\u5b58\u64cd\u4f5c\uff08\u91cd\u70b9\u4fdd\u7559\u62a5\u544a\u57fa\u56e0\u529f\u80fd\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u9009\u53d6\u5bf9\u6570\u671f\uff08\u589e\u6b96\u65fa\u76db\u3001\u4e0a\u76ae\u5f62\u6001\u5747\u4e00\u3001\u65e0\u6742\u7ec6\u80de\uff0cNluc \u8367\u5149\u4fe1\u53f7\u7a33\u5b9a\uff09\u7684\u7ec6\u80de\uff0c\u80f0\u9176\u6d88\u5316\u540e 1000rpm \u79bb\u5fc3 5 \u5206\u949f\uff0c\u5f03\u4e0a\u6e05\u6536\u96c6\u7ec6\u80de\u6c89\u6dc0\u3002<\/li>\n<li>\u7528\u63a8\u8350\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff08IMC-701\uff09\u91cd\u60ac\u7ec6\u80de\u81f3 1\u00d710\u2076-2\u00d710\u2076cells\/ml\uff0c\u5206\u88c5\u5230\u51bb\u5b58\u7ba1\u540e\uff0c\u6309 \u201c4\u2103\u9759\u7f6e 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u8f6c\u5165\u6db2\u6c2e\u201d \u7684\u68af\u5ea6\u964d\u6e29\uff0c\u51cf\u5c11\u4f4e\u6e29\u5bf9\u7a33\u8f6c\u7ec6\u80de\u7684\u635f\u4f24\uff0c\u4fdd\u7559 FGFR1 \u57fa\u56e0\u4e0e Nluc \u62a5\u544a\u57fa\u56e0\u529f\u80fd\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">4. \u590d\u82cf\u64cd\u4f5c\uff08\u91cd\u70b9\u51cf\u5c11\u7ec6\u80de\u5e94\u6fc0\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u4ece\u6db2\u6c2e\u4e2d\u53d6\u51fa\u51bb\u5b58\u7ba1\uff0c\u7acb\u5373\u653e\u5165 37\u2103\u6c34\u6d74\u901f\u878d\uff081-2 \u5206\u949f\u5185\u5b8c\u5168\u878d\u5316\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u7834\u574f\u7ec6\u80de\u4e0e\u7a33\u8f6c\u57fa\u56e0\uff09\uff0c\u8fc5\u901f\u5c06\u7ec6\u80de\u60ac\u6db2\u8f6c\u79fb\u81f3\u79bb\u5fc3\u7ba1\u3002<\/li>\n<li>\u52a0\u5165 5 \u500d\u4f53\u79ef\u65b0\u9c9c\u65e0\u836f MEM \u57f9\u517b\u57fa\uff08\u6682\u4e0d\u52a0 puro\/bsd\uff0c\u964d\u4f4e\u590d\u82cf\u521d\u671f\u7ec6\u80de\u5e94\u6fc0\uff09\u7a00\u91ca\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u5f03\u4e0a\u6e05\uff0c\u7528\u542b 1.0\u03bcg\/ml puro \u4e0e 4.0\u03bcg\/ml bsd \u7684\u9884\u70ed\u57f9\u517b\u57fa\u91cd\u60ac\u7ec6\u80de\u540e\u63a5\u79cd\uff1b\u590d\u82cf\u540e 24 \u5c0f\u65f6\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u4e0e\u8367\u5149\u4fe1\u53f7\uff0c48 \u5c0f\u65f6\u540e\u6309\u5e38\u89c4\u6362\u6db2\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">5. FGF1 \u6fc0\u6d3b\u9a8c\u8bc1\u5b9e\u9a8c\u914d\u5957\u64cd\u4f5c<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u5b9e\u9a8c\u524d\u5c06\u7ec6\u80de\u63a5\u79cd\u81f3 6 \u5b54\u677f\uff08QPCR \u7528\uff09\u6216\u7ec6\u80de\u57f9\u517b\u76bf\uff08WB \u7528\uff09\uff0c\u5f85\u5bc6\u5ea6\u8fbe 50%-60% \u65f6\uff0c\u66f4\u6362\u542b\u4e0d\u540c\u6d53\u5ea6 FGF1 \u523a\u6fc0\u5242\u7684\u65e0\u836f\u57f9\u517b\u57fa\uff0c\u6309\u968f\u8d27\u8bf4\u660e\u4e66\u8bbe\u5b9a\u57f9\u517b\u65f6\u95f4\uff08\u901a\u5e38\u4e3a 8-24 \u5c0f\u65f6\uff09\u3002<\/li>\n<li>QPCR \u68c0\u6d4b\uff1a\u6536\u96c6\u7ec6\u80de\u63d0\u53d6\u603b RNA\uff0c\u9006\u8f6c\u5f55\u4e3a cDNA \u540e\uff0c\u901a\u8fc7\u7279\u5f02\u6027\u5f15\u7269\u68c0\u6d4b FGF1 \u6fc0\u6d3b\u76f8\u5173\u9776\u57fa\u56e0\uff08\u5982 FGFR1 \u4e0b\u6e38\u901a\u8def\u5206\u5b50\uff09\u7684\u8868\u8fbe\u6c34\u5e73\u3002<\/li>\n<li>WB \u68c0\u6d4b\uff1a\u6536\u96c6\u7ec6\u80de\u63d0\u53d6\u603b\u86cb\u767d\uff0c\u7ecf SDS-PAGE \u7535\u6cf3\u3001\u8f6c\u819c\u540e\uff0c\u5b75\u80b2\u6297 FGFR1 \u4e00\u6297\u4e0e\u8367\u5149\u4e8c\u6297\uff0c\u663e\u5f71\u68c0\u6d4b FGFR1 \u86cb\u767d\u8868\u8fbe\u53ca\u78f7\u9178\u5316\u6c34\u5e73\uff08\u82e5\u9700\u5206\u6790\u901a\u8def\u6d3b\u6027\uff09\u3002<\/li>\n<li>\u5b9e\u9a8c\u9700\u8bbe\u7f6e\u7a7a\u767d\u5bf9\u7167\u7ec4\uff08\u65e0 FGF1 \u523a\u6fc0\uff09\u4e0e\u9633\u6027\u5bf9\u7167\u7ec4\uff08\u5df2\u77e5\u6709\u6548\u6d53\u5ea6 FGF1 \u523a\u6fc0\uff09\uff0c\u786e\u4fdd\u7ed3\u679c\u7684\u53ef\u9760\u6027\u4e0e\u91cd\u590d\u6027\u3002<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aHEK293-FGFR1 (Nluc) \u4eba\u80be<\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/hek293-fgfr1nluc\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        <\/div>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[18],"product_tag":[],"class_list":{"0":"post-1615","1":"product","2":"type-product","3":"status-publish","5":"product_cat-18","7":"first","8":"instock","9":"sale","10":"taxable","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product\/1615","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/comments?post=1615"}],"wp:attachment":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/media?parent=1615"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=1615"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=1615"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=1615"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}