{"id":164,"date":"2025-08-23T16:45:18","date_gmt":"2025-08-23T08:45:18","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=164"},"modified":"2025-08-23T16:45:19","modified_gmt":"2025-08-23T08:45:19","slug":"wpmy-1%e4%ba%ba%e6%ad%a3%e5%b8%b8%e5%89%8d%e5%88%97%e8%85%ba%e5%9f%ba%e8%b4%a8%e6%b0%b8%e7%94%9f%e5%8c%96%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/wpmy-1%e4%ba%ba%e6%ad%a3%e5%b8%b8%e5%89%8d%e5%88%97%e8%85%ba%e5%9f%ba%e8%b4%a8%e6%b0%b8%e7%94%9f%e5%8c%96%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"WPMY-1\u4eba\u6b63\u5e38\u524d\u5217\u817a\u57fa\u8d28\u6c38\u751f\u5316\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u540d\u79f0\uff1aWPMY-1 \u4eba\u6b63\u5e38\u524d\u5217\u817a\u57fa\u8d28\u6c38\u751f\u5316\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff08\u4e0e VCaP\u3001PC-3 \u7b49\u524d\u5217\u817a\u764c\u7ec6\u80de\u5f62\u6210 \u201c\u6b63\u5e38\u57fa\u8d28 &#8211; \u80bf\u7624\u7ec6\u80de\u201d \u6838\u5fc3\u5bf9\u7167\uff0c\u4e14\u662f\u524d\u5217\u817a\u57fa\u8d28\u7ec6\u80de\uff08\u975e\u4e0a\u76ae\u7ec6\u80de\uff09\uff0c\u533a\u522b\u4e8e RWPE-1\uff08\u540c\u6765\u6e90\u524d\u5217\u817a\u4e0a\u76ae\u7ec6\u80de\uff09\uff0c\u662f\u7814\u7a76\u524d\u5217\u817a\u57fa\u8d28 &#8211; \u4e0a\u76ae\u7ec6\u80de\u4ea4\u4e92\u4f5c\u7528\u3001\u80bf\u7624\u5fae\u73af\u5883\u8c03\u63a7\u7684\u5173\u952e\u6b63\u5e38\u6a21\u578b\uff1b\u53ef\u4e0e VCaP\uff08\u810a\u690e\u8f6c\u79fb\u764c\uff09\u3001PC-3\uff08\u9aa8\u8f6c\u79fb\u764c\uff09\u7ec4\u6210 \u201c\u57fa\u8d28 &#8211; \u80bf\u7624\u201d \u5171\u57f9\u517b\u4f53\u7cfb\uff0c\u63a2\u7d22\u524d\u5217\u817a\u764c\u8f6c\u79fb\u8fc7\u7a0b\u4e2d\u57fa\u8d28\u7ec6\u80de\u7684\u8c03\u63a7\u673a\u5236\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u522b\u79f0\uff1aWPMY1\uff1bWPMY-1\uff08\u522b\u79f0\u7b80\u6d01\uff0c\u4ee5 \u201cWPMY\u201d \u4e3a\u6838\u5fc3\u6807\u8bc6\uff0c\u65e0\u590d\u6742\u540e\u7f00\uff0c\u4e0e VCaP\uff08\u201cVCaP\u201d \u524d\u7f00\uff09\u3001PC-3\uff08\u201cPC\u201d \u524d\u7f00\uff09\u5f62\u6210\u524d\u5217\u817a\u7ec6\u80de\u682a\u7684\u6e05\u6670\u533a\u5206\uff0c\u6570\u5b57 \u201c1\u201d \u4fbf\u4e8e\u5b9e\u9a8c\u8bb0\u5f55\u53ca\u6587\u732e\u68c0\u7d22\uff0c\u8d34\u5408 \u201c\u6b63\u5e38\u7ec6\u80de + \u7f16\u53f7\u201d \u7684\u547d\u540d\u4f53\u7cfb\uff0c\u540c\u65f6\u907f\u514d\u4e0e\u5176\u4ed6 \u201cWP\u201d \u524d\u7f00\u7ec6\u80de\uff08\u5982\u80ba\u7ec6\u80de\uff09\u6df7\u6dc6\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u6027\u522b\u5e74\u9f84\uff1a\u7537\uff1b54 \u5c81\uff08\u660e\u786e\u7684\u4e2d\u5e74\u7537\u6027\u80cc\u666f\uff0c\u4e0e VCaP\uff08\u63a8\u6d4b\u4e2d\u8001\u5e74\u7537\uff09\u3001PC-3\uff0862 \u5c81\u7537\uff09\u5f62\u6210 \u201c\u4e2d\u5e74 &#8211; \u8001\u5e74\u201d \u5e74\u9f84\u68af\u5ea6\uff0c\u4e0e LNCaP\uff0850 \u5c81\u7537\uff09\u5e74\u9f84\u76f8\u8fd1\uff0c\u4e3a\u524d\u5217\u817a\u6b63\u5e38\u57fa\u8d28\u7ec6\u80de\u4e0e\u80bf\u7624\u7ec6\u80de\u7684\u5e74\u9f84\u5339\u914d\u5bf9\u7167\u63d0\u4f9b\u7cbe\u51c6\u6a21\u578b\uff0c\u9002\u5408\u7814\u7a76\u5e74\u9f84\u76f8\u5173\u7684\u57fa\u8d28 &#8211; \u80bf\u7624\u4ea4\u4e92\u4f5c\u7528\u5dee\u5f02\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec4\u7ec7\u6765\u6e90\uff1a\u524d\u5217\u817a \/ \u57fa\u8d28\uff08\u6838\u5fc3\u7279\u8272\u4e3a\u6b63\u5e38\u524d\u5217\u817a\u57fa\u8d28\u7ec4\u7ec7\u6765\u6e90\uff0c\u4e14\u4e0e RWPE-1 \u7ec6\u80de\uff08\u540c\u6765\u6e90\u524d\u5217\u817a\u4e0a\u76ae\u7ec6\u80de\uff09\u6e90\u81ea\u540c\u4e00\u5f20\u6210\u4eba\u524d\u5217\u817a\u7ec4\u7ec7\u5207\u7247\u7684\u5468\u56f4\u533a\u57df\uff0c\u4fdd\u7559\u6b63\u5e38\u524d\u5217\u817a\u57fa\u8d28\u7ec6\u80de\u7279\u6709\u7684 \u201c\u5206\u6ccc\u529f\u80fd\u3001\u7ec6\u80de\u5916\u57fa\u8d28\u5408\u6210\u3001\u4e0e\u4e0a\u76ae\u7ec6\u80de\u4ea4\u4e92\u4f5c\u7528\u201d \u7279\u6027\uff1b\u533a\u522b\u4e8e\u6240\u6709\u524d\u5217\u817a\u764c\u7ec6\u80de\u7684\u80bf\u7624\u7ec4\u7ec7\u6765\u6e90\uff0c\u53ef\u7528\u4e8e\u524d\u5217\u817a\u6b63\u5e38\u751f\u7406\u529f\u80fd\uff08\u5982\u57fa\u8d28\u652f\u6301\u4e0a\u76ae\u5206\u5316\uff09\u3001\u80bf\u7624\u5fae\u73af\u5883\uff08\u5982\u57fa\u8d28\u7ec6\u80de\u4fc3\u8fdb\u764c\u8f6c\u79fb\uff09\u7814\u7a76\uff0c\u540c\u65f6\u4e3a\u4e34\u5e8a\u524d\u5217\u817a\u57fa\u8d28\u76f8\u5173\u75be\u75c5\uff08\u5982\u826f\u6027\u524d\u5217\u817a\u589e\u751f\uff09\u63d0\u4f9b\u5b9e\u9a8c\u4f9d\u636e\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f\uff08\u8d34\u58c1\u80fd\u529b\u7a33\u5b9a\uff0c\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\u589e\u6b96\u8282\u594f\u5e73\u7f13\uff0c\u4e0e\u6b63\u5e38\u57fa\u8d28\u7ec6\u80de\u4ee3\u8c22\u7279\u6027\u5339\u914d\uff0c\u8d34\u58c1\u5f3a\u5ea6\u9ad8\u4e8e VCaP\uff08\u8d34\u58c1\u5dee\uff09\u3001MDA-PCA-2B\uff08\u8d34\u58c1\u5dee\uff09\uff0c\u4f4e\u4e8e PC-3\uff08\u8d34\u58c1\u7a33\u5b9a\uff09\uff1b\u7ec6\u80de\u751f\u957f\u65e0\u6210\u7c07\u6216\u6210\u56e2\u73b0\u8c61\uff0c\u5448\u5747\u5300\u5355\u5c42\u5206\u5e03\uff0c\u4f20\u4ee3\u64cd\u4f5c\u53ef\u53c2\u8003\u6b63\u5e38\u57fa\u8d28\u7ec6\u80de\u901a\u7528\u6d41\u7a0b\uff0c\u4fbf\u4e8e\u4e0e\u80bf\u7624\u7ec6\u80de\u540c\u6b65\u5f00\u5c55\u5171\u57f9\u517b\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u5f62\u6001\uff1a\u6210\u808c\u7ec6\u80de\u6837\uff08\u6838\u5fc3\u5f62\u6001\u7279\u8272\u4e3a\u6210\u808c\u7ec6\u80de\u6837\uff0c\u5448\u957f\u68ad\u5f62\uff0c\u80de\u8d28\u7ec6\u957f\uff0c\u6838\u5448\u692d\u5706\u5f62\uff0c\u6392\u5217\u5448\u5e73\u884c\u6216\u6f29\u6da1\u72b6\uff0c\u4e0e\u6240\u6709\u524d\u5217\u817a\u764c\u7ec6\u80de\u7684 \u201c\u4e0a\u76ae\u7ec6\u80de\u6837\u201d \u5f62\u6001\u663e\u8457\u5dee\u5f02\uff1b\u4e0e RWPE-1\uff08\u4e0a\u76ae\u7ec6\u80de\u6837\uff09\u76f8\u6bd4\uff0c\u5f62\u6001\u662f\u9274\u522b\u57fa\u8d28\u4e0e\u4e0a\u76ae\u7ec6\u80de\u7684\u5173\u952e\u6807\u5fd7\uff0c\u53ef\u901a\u8fc7\u5f62\u6001\u89c2\u5bdf\u5feb\u901f\u5224\u65ad\u7ec6\u80de\u7eaf\u5ea6\uff0c\u907f\u514d\u4e0e\u4e0a\u76ae\u7ec6\u80de\u6216\u80bf\u7624\u7ec6\u80de\u4ea4\u53c9\u6c61\u67d3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4ee3\u6570\u7ec6\u80de\u80fd\u66f4\u597d\u4fdd\u7559 \u201c\u6b63\u5e38\u57fa\u8d28\u7279\u6027\u3001SV40 \u6c38\u751f\u5316\u7a33\u5b9a\u6027\u3001\u4e0e RWPE-1 \u7684\u4ea4\u4e92\u80fd\u529b\u201d \u6838\u5fc3\u7279\u5f81\uff0c\u5efa\u8bae\u4f20\u4ee3\u6b21\u6570\u4e0d\u8d85\u8fc7 15 \u4ee3\uff0c\u9632\u6b62\u957f\u671f\u4f20\u4ee3\u5bfc\u81f4\u7279\u6027\u6f02\u79fb\uff0c\u5982\u6210\u808c\u7ec6\u80de\u6837\u5f62\u6001\u4e22\u5931\u3001\u5206\u6ccc\u529f\u80fd\u4e0b\u964d\u3001\u4e0e\u4e0a\u76ae\u7ec6\u80de\u4ea4\u4e92\u80fd\u529b\u51cf\u5f31\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u80cc\u666f\u4ecb\u7ecd\uff1aWPMY-1 \u7ec6\u80de\u4e0e RWPE-1 \u7ec6\u80de\u540c\u6e90\u4e8e\u540c\u4e00\u5f20\u6210\u4eba\u524d\u5217\u817a\u7ec4\u7ec7\u5207\u7247\u7684\u5468\u56f4\u533a\u57df\uff0c\u901a\u8fc7 pRSTV \u8d28\u7c92\u5bfc\u5165 SV40 \u5927 T \u6297\u539f\u5b9e\u73b0\u6c38\u751f\u5316\uff0c\u6838\u5fc3\u7279\u6027\u53ca\u5e94\u7528\u5982\u4e0b\uff1a\u2460\u6c38\u751f\u5316\u4e0e\u540c\u6e90\u6027\u7279\u8272\uff1a\u6c38\u751f\u5316\u8fc7\u7a0b\u53ef\u8ffd\u6eaf\uff08SV40 \u5927 T \u6297\u539f\u4ecb\u5bfc\uff09\uff0c\u514b\u670d\u539f\u4ee3\u57fa\u8d28\u7ec6\u80de\u4f20\u4ee3\u6b21\u6570\u6709\u9650\u7684\u7f3a\u9677\uff1b\u4e0e RWPE-1 \u7684\u540c\u6e90\u6027\u4f7f\u5176\u6210\u4e3a\u7814\u7a76\u524d\u5217\u817a\u57fa\u8d28 &#8211; \u4e0a\u76ae\u7ec6\u80de\u4ea4\u4e92\u4f5c\u7528\u7684 \u201c\u9ec4\u91d1\u914d\u5bf9\u201d \u6a21\u578b\uff0c\u53ef\u6a21\u62df\u4f53\u5185\u524d\u5217\u817a\u7ec4\u7ec7\u7684\u57fa\u8d28 &#8211; \u4e0a\u76ae\u7ed3\u6784\uff1b\u2461\u5b89\u5168\u6027\u9a8c\u8bc1\uff1a\u540c\u6e90\u7ec6\u80de RWPE-1 \u7ecf\u68c0\u6d4b\u4e59\u809d\u3001\u4e19\u809d\u3001\u4eba\u514d\u75ab\u7f3a\u9677\u75c5\u6bd2\u5747\u4e3a\u9634\u6027\uff0c\u95f4\u63a5\u4f50\u8bc1 WPMY-1 \u7ec6\u80de\u7684\u751f\u7269\u5b89\u5168\u6027\uff0c\u964d\u4f4e\u5b9e\u9a8c\u611f\u67d3\u98ce\u9669\uff1b\u2462\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u524d\u5217\u817a\u6b63\u5e38\u57fa\u8d28\u529f\u80fd\u7814\u7a76\uff08\u5982\u5206\u6ccc\u7ec6\u80de\u56e0\u5b50\u8c03\u63a7\u4e0a\u76ae\u5206\u5316\uff09\u3001\u524d\u5217\u817a\u764c\u5fae\u73af\u5883\u673a\u5236\u7814\u7a76\uff08\u5982\u57fa\u8d28\u7ec6\u80de\u5206\u6ccc\u56e0\u5b50\u4fc3\u8fdb VCaP \u810a\u690e\u8f6c\u79fb\uff09\u3001\u57fa\u8d28 &#8211; \u4e0a\u76ae\u5171\u57f9\u517b\u6a21\u578b\u6784\u5efa\uff08\u4e0e RWPE-1\uff09\uff0c\u540c\u65f6\u53ef\u4e0e PC-3\uff08\u9aa8\u8f6c\u79fb\u764c\uff09\u642d\u914d\u5f00\u5c55 \u201c\u57fa\u8d28 &#8211; \u9aa8\u8f6c\u79fb\u764c\u201d \u5171\u57f9\u517b\uff0c\u63a2\u7d22\u9aa8\u8f6c\u79fb\u8fc7\u7a0b\u4e2d\u524d\u5217\u817a\u57fa\u8d28\u7684\u8fdc\u7a0b\u8c03\u63a7\u4f5c\u7528\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a2\uff08\u56e0\u542b SV40 \u5927 T \u6297\u539f\u6c38\u751f\u5316\u80cc\u666f\uff0c\u867d\u65e0\u4f20\u67d3\u6027\u75c5\u6bd2\uff0c\u4f46\u5b58\u5728\u5916\u6e90\u57fa\u56e0\u6f5c\u5728\u98ce\u9669\uff0c\u751f\u7269\u5b89\u5168\u7b49\u7ea7\u4e0e VCaP\uff08\u7b49\u7ea7 2\uff09\u4e00\u81f4\uff0c\u9ad8\u4e8e PC-3\u3001LNCaP \u7684\u7b49\u7ea7 1\uff1b\u64cd\u4f5c\u9700\u5728\u751f\u7269\u5b89\u5168\u4e8c\u7ea7\u5b9e\u9a8c\u5ba4\u8fdb\u884c\uff0c\u7a7f\u6234\u9632\u62a4\u670d\u3001\u624b\u5957\uff0c\u4f7f\u7528\u5e26\u6ee4\u82af\u5438\u5934\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\uff0c\u907f\u514d\u5916\u6e90\u57fa\u56e0\u6269\u6563\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u5b58\u6d3b\u7387\u7a33\u5b9a\u5728 85% \u4ee5\u4e0a\uff0c\u4e14\u6210\u808c\u7ec6\u80de\u6837\u5f62\u6001\u3001\u5206\u6ccc\u529f\u80fd\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%-80%\uff0c\u786e\u4fdd\u5230\u8fbe\u540e\u53ef\u76f4\u63a5\u7528\u4e8e\u5b9e\u9a8c\u6216\u4f20\u4ee3\uff0c\u65e0\u9700\u989d\u5916\u57f9\u517b\u7b49\u5f85\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u751f\u957f\u53ca\u5206\u6ccc\u529f\u80fd\uff0c\u4fdd\u969c\u57fa\u8d28 &#8211; \u80bf\u7624\u5171\u57f9\u517b\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CRL-2854\uff1b\u4e2d\u56fd\u5178\u578b\u57f9\u517b\u7269\u4fdd\u85cf\u4e2d\u5fc3\u7ec6\u80de\u5e93\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\uff09\u4e0e\u56fd\u5185\u673a\u6784\u53cc\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\uff0c\u6b63\u5e38\u57fa\u8d28\u7279\u6027\u53ca\u6c38\u751f\u5316\u7a33\u5b9a\u6027\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4e0e VCaP\uff08ATCC CRL-2876\uff09\u3001PC-3\uff08ATCC CRL-1435\uff09\u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u8de8\u5730\u57df\u5b9e\u9a8c\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1aDMEM+5% FBS+1% \u53cc\u6297\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528\u4f4e\u8840\u6e05\uff085% FBS\uff09DMEM \u57f9\u517b\u57fa\uff0c\u533a\u522b\u4e8e VCaP\uff0810% FBS DMEM\uff09\u3001PC-3\uff0810% FBS F12K\uff09\uff1b\u4f4e\u8840\u6e05\u914d\u65b9\u9002\u914d\u6b63\u5e38\u57fa\u8d28\u7ec6\u80de\u751f\u957f\uff0c\u907f\u514d\u9ad8\u8840\u6e05\u8bf1\u5bfc\u7ec6\u80de\u6d3b\u5316\u6216\u5206\u5316\uff0c\u540c\u65f6\u7ef4\u6301 SV40 \u5927 T \u6297\u539f\u7684\u7a33\u5b9a\u8868\u8fbe\uff1b\u2461FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u7ec6\u80de\u56e0\u5b50\u5e72\u6270\u57fa\u8d28\u7ec6\u80de\u7684\u6b63\u5e38\u5206\u6ccc\u529f\u80fd\uff1b\u2462\u53cc\u6297\u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u6c38\u751f\u5316\u7279\u6027\u53ca\u57fa\u8d28\u529f\u80fd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u57f9\u517b\u7bb1\u5185\u6258\u76d8\u9700\u6c34\u5e73\u653e\u7f6e\uff0c\u907f\u514d\u57f9\u517b\u57fa\u503e\u659c\u5bfc\u81f4\u7ec6\u80de\u5206\u5e03\u4e0d\u5747\uff1b\u4e0e VCaP\u3001PC-3\u3001LNCaP \u7b49\u6240\u6709\u524d\u5217\u817a\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u4ec5\u9700\u5728\u5171\u57f9\u517b\u5b9e\u9a8c\u4e2d\u8c03\u6574\u63a5\u79cd\u6bd4\u4f8b\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u6b63\u5e38\u57fa\u8d28\u7ec6\u80de\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u8d34\u58c1\u5747\u5300\u3001\u65e0\u8001\u5316\u8ff9\u8c61\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 3-4 \u5206\u949f\uff08\u957f\u4e8e\u80bf\u7624\u7ec6\u80de\uff09\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u3001\u68ad\u5f62\u6536\u7f29\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff08\u4fdd\u62a4\u6210\u808c\u7ec6\u80de\u6837\u5f62\u6001\uff09\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 1\u00d710\u2076-2\u00d710\u2076cells\/mL\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 DMEM \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\uff0c\u51cf\u5c11\u5bf9\u57fa\u8d28\u7ec6\u80de\u5f62\u6001\u53ca\u529f\u80fd\u7684\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7\u4f4e\u8840\u6e05\u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001\u6210\u808c\u7ec6\u80de\u6837\u5f62\u6001\u9a8c\u8bc1\u53ca\u4e0e RWPE-1 \u7684\u4ea4\u4e92\u80fd\u529b\u9884\u5b9e\u9a8c\uff0c\u786e\u4fdd\u6b63\u5e38\u57fa\u8d28\u7279\u6027\u7a33\u5b9a\uff1b\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u53ef\u4e0e VCaP\u3001PC-3 \u540c\u6b65\u4e0b\u5355\uff0c\u4fdd\u969c \u201c\u57fa\u8d28 &#8211; \u80bf\u7624\u201d \u5171\u57f9\u517b\u5b9e\u9a8c\u7684\u65f6\u95f4\u4e00\u81f4\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u907f\u514d\u5267\u70c8\u98a0\u7c38\uff0c\u51cf\u5c11\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165 37\u2103\u57f9\u517b\u7bb1\uff0c24 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\uff0c\u65e0\u9700\u7279\u6b8a\u9759\u7f6e\u5904\u7406\uff0c3 \u5929\u5185\u9996\u6b21\u6362\u6db2\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 500g-1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226550%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u65e5\u8fbe\u670d\u52a1\uff0c\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca\u6c38\u751f\u5316\u7279\u6027\u6539\u53d8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e VCaP\u3001PC-3 \u7b49\u7ec6\u80de\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff1b\u56e0\u542b SV40 \u5927 T \u6297\u539f\uff0c\u9700\u9075\u5b88\u300a\u57fa\u56e0\u5de5\u7a0b\u5b89\u5168\u7ba1\u7406\u529e\u6cd5\u300b\uff0c\u5b9e\u9a8c\u64cd\u4f5c\u53ca\u5e9f\u5f03\u7269\u5904\u7406\u7b26\u5408\u751f\u7269\u5b89\u5168\u4e8c\u7ea7\u5b9e\u9a8c\u5ba4\u8981\u6c42\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460\u57f9\u517b\u57fa\u4e0e\u8840\u6e05\u63a7\u5236\uff1a\u4e25\u683c\u4f7f\u7528 \u201cDMEM+5% FBS+1% \u53cc\u6297\u201d \u914d\u65b9\uff0c\u4e0d\u53ef\u4e0e VCaP \u7684 10% FBS DMEM\u3001PC-3 \u7684 10% FBS F12K \u6df7\u7528\uff1b\u4f4e\u8840\u6e05\uff085%\uff09\u662f\u7ef4\u6301\u6b63\u5e38\u57fa\u8d28\u7ec6\u80de\u7279\u6027\u7684\u5173\u952e\uff0c\u82e5\u4f7f\u7528 10% FBS \u53ef\u80fd\u5bfc\u81f4\u7ec6\u80de\u6d3b\u5316\uff08\u5982 \u03b1-SMA \u8868\u8fbe\u5347\u9ad8\uff09\uff0c\u5f71\u54cd\u4e0e\u4e0a\u76ae\u7ec6\u80de\u7684\u4ea4\u4e92\u529f\u80fd\uff1b\u2461\u5171\u57f9\u517b\u5b9e\u9a8c\u8bbe\u8ba1\uff1a\u4e0e RWPE-1\uff08\u4e0a\u76ae\u7ec6\u80de\uff09\u5f00\u5c55\u57fa\u8d28 &#8211; \u4e0a\u76ae\u5171\u57f9\u517b\u65f6\uff0c\u5efa\u8bae\u6309 1:1 \u6bd4\u4f8b\u63a5\u79cd\uff0c\u4f7f\u7528 Transwell \u5c0f\u5ba4\uff08\u57fa\u8d28\u7ec6\u80de\u79cd\u4e8e\u4e0b\u5ba4\uff0c\u4e0a\u76ae\u7ec6\u80de\u79cd\u4e8e\u4e0a\u5ba4\uff09\uff0c\u57f9\u517b 7-10 \u5929\u89c2\u5bdf\u4e0a\u76ae\u7ec6\u80de\u5206\u5316\u60c5\u51b5\uff1b\u4e0e VCaP\uff08\u80bf\u7624\u7ec6\u80de\uff09\u5f00\u5c55\u5171\u57f9\u517b\u65f6\uff0c\u53ef\u91c7\u7528\u76f4\u63a5\u5171\u57f9\u517b\uff08\u6309 2:1 \u57fa\u8d28 &#8211; \u80bf\u7624\u6bd4\u4f8b\uff09\uff0c\u68c0\u6d4b\u57fa\u8d28\u7ec6\u80de\u5bf9\u80bf\u7624\u7ec6\u80de\u4fb5\u88ad\u80fd\u529b\uff08Transwell \u5b9e\u9a8c\uff09\u7684\u5f71\u54cd\uff0c\u8bbe\u7f6e\u5355\u72ec\u80bf\u7624\u7ec6\u80de\u7ec4\u4f5c\u4e3a\u5bf9\u7167\uff1b\u2462\u5f62\u6001\u4e0e\u529f\u80fd\u9a8c\u8bc1\uff1a\u5b9e\u9a8c\u524d\u9700\u901a\u8fc7\u514d\u75ab\u8367\u5149\u68c0\u6d4b\u6210\u808c\u7ec6\u80de\u6807\u5fd7\u7269\uff08\u5982 desmin\u3001vimentin\uff09\uff0c\u786e\u8ba4\u7ec6\u80de\u57fa\u8d28\u8eab\u4efd\uff0c\u6392\u9664\u4e0a\u76ae\u7ec6\u80de\u6216\u80bf\u7624\u7ec6\u80de\u6c61\u67d3\uff1b\u68c0\u6d4b\u57fa\u8d28\u7ec6\u80de\u5206\u6ccc\u529f\u80fd\uff08\u5982 IL-6\u3001TGF-\u03b2\uff09\u65f6\uff0c\u6536\u96c6\u57f9\u517b\u4e0a\u6e05\uff0c\u7528 ELISA \u6cd5\u68c0\u6d4b\uff0c\u9a8c\u8bc1\u7ec6\u80de\u529f\u80fd\u7a33\u5b9a\u6027\uff1b\u2463\u4e0e\u80bf\u7624\u7ec6\u80de\u7684\u5bf9\u6bd4\u5b9e\u9a8c\uff1a\u642d\u914d PC-3 \u5f00\u5c55 \u201c\u6b63\u5e38\u57fa\u8d28 &#8211; \u9aa8\u8f6c\u79fb\u764c\u201d \u7814\u7a76\u65f6\uff0c\u53ef\uff1aa. \u68c0\u6d4b\u57fa\u8d28\u7ec6\u80de\u5206\u6ccc\u7684\u9aa8\u76f8\u5173\u56e0\u5b50\uff08\u5982 OPG\u3001RANKL\uff09\u5bf9\u80bf\u7624\u7ec6\u80de\u9aa8\u9ecf\u9644\u80fd\u529b\u7684\u5f71\u54cd\uff1bb. \u5bf9\u6bd4\u4e0d\u540c\u8f6c\u79fb\u90e8\u4f4d\u80bf\u7624\u7ec6\u80de\uff08VCaP \u810a\u690e\u8f6c\u79fb\u3001PC-3 \u9aa8\u8f6c\u79fb\uff09\u4e0e\u57fa\u8d28\u7ec6\u80de\u7684\u4ea4\u4e92\u4f5c\u7528\u5dee\u5f02\uff0c\u5206\u6790\u8f6c\u79fb\u5668\u5b98\u7279\u5f02\u6027\u7684\u5fae\u73af\u5883\u673a\u5236\uff1b\u2464\u957f\u671f\u57f9\u517b\u76d1\u6d4b\uff1a\u6bcf 5 \u4ee3\u68c0\u6d4b\u6210\u808c\u7ec6\u80de\u6837\u5f62\u6001\u3001SV40 \u5927 T \u6297\u539f\u8868\u8fbe\uff08Western Blot\uff09\u53ca\u5206\u6ccc\u529f\u80fd\uff0c\u82e5\u53d1\u73b0\u5f62\u6001\u68ad\u5f62\u53d8\u5706\u3001\u5206\u6ccc\u56e0\u5b50\u4e0b\u964d\uff0c\u9700\u7acb\u5373\u66f4\u6362\u4f4e\u4ee3\u6570\u79cd\u5b50\u7ec6\u80de\uff0c\u786e\u4fdd\u5b9e\u9a8c\u7ed3\u679c\u53ef\u9760\u3002<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"21\" data-line=\"true\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027 \u7ec6\u80de\u540d\u79f0\uff1aWPMY-1 \u4eba\u6b63\u5e38\u524d\u5217\u817a\u57fa\u8d28\u6c38\u751f\u5316\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff08\u4e0e VCaP\u3001PC-<\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/wpmy-1%e4%ba%ba%e6%ad%a3%e5%b8%b8%e5%89%8d%e5%88%97%e8%85%ba%e5%9f%ba%e8%b4%a8%e6%b0%b8%e7%94%9f%e5%8c%96%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        <\/div>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[17],"product_tag":[],"class_list":{"0":"post-164","1":"product","2":"type-product","3":"status-publish","5":"product_cat-17","7":"first","8":"instock","9":"sale","10":"taxable","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product\/164","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/comments?post=164"}],"wp:attachment":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/media?parent=164"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=164"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=164"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=164"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}