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class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin:X\uff08\u4e0e\u5973\u6027\u6027\u522b\u5339\u914d\uff0c\u9a8c\u8bc1\u7ec6\u80de\u6027\u522b\u4e00\u81f4\u6027\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff0c\u4e0e Eca-109 \u7684 Amelogenin:X X \u5171\u540c\u786e\u8ba4\u5973\u6027\u6d88\u5316\u9053\u80bf\u7624\u7ec6\u80de\u8eab\u4efd\uff09; CSF1PO:11,12\uff1bD13S317:12\uff1bD16S539:11,13\uff1bD18S51:13\uff1bD19S433:13.2,16\uff1bD21S11:29\uff1bD2S1338:20,22\uff1bD3S1358:16\uff1bD5S818:9,12\uff1bD7S820:10,11\uff1bD8S1179:13\uff1bFGA:23,24\uff1bTH01:6,7\uff1bTPOX:11,12\uff1bvWA:16,17\uff08STR \u4f4d\u70b9\u542b\u7279\u5f02\u6027\u6742\u5408\u5b50\u4f4d\u70b9\uff08\u5982 D2S1338:20,22\u3001FGA:23,24\uff09\uff0c\u4e0e TE-1\u3001Eca-109 \u7684 STR \u56fe\u8c31\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7\u56fe\u8c31\u4e0e\u4fdd\u85cf\u673a\u6784\uff08ATCC CRL-1739\uff09\u6570\u636e\u6bd4\u5bf9\uff0c\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\u53ca\u80c3\u817a\u764c\u7279\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a2\uff08\u9ad8\u4e8e TE-1\u3001Eca-109 \u7684\u7b49\u7ea7 1\uff0c\u64cd\u4f5c\u9700\u5728\u751f\u7269\u5b89\u5168\u4e8c\u7ea7\u5b9e\u9a8c\u5ba4\u8fdb\u884c\uff0c\u7a7f\u6234\u9632\u62a4\u670d\u3001\u624b\u5957\uff0c\u4f7f\u7528\u5e26\u6ee4\u82af\u5438\u5934\uff1b\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u907f\u514d\u751f\u7269\u5371\u5bb3\uff0c\u4e0e WPMY-1\u3001VCaP \u7684\u5b89\u5168\u9632\u62a4\u8981\u6c42\u4e00\u81f4\uff0c\u4fbf\u4e8e\u5b9e\u9a8c\u5ba4\u7edf\u4e00\u7ba1\u7406\u4e0d\u540c\u5b89\u5168\u7b49\u7ea7\u7ec6\u80de\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u5b58\u6d3b\u7387\u7a33\u5b9a\u5728 85% \u4ee5\u4e0a\uff0c\u4e14\u80c3\u817a\u764c\u5f62\u6001\u3001\u77ed\u500d\u589e\u65f6\u95f4\u3001\u690d\u677f\u7387\u7279\u6027\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%\uff08\u56e0\u589e\u6b96\u5feb\uff0c\u907f\u514d\u5bc6\u5ea6\u8fc7\u9ad8\u5bfc\u81f4\u4f20\u4ee3\u5ef6\u8bef\uff09\uff0c\u786e\u4fdd\u5230\u8fbe\u540e\u53ef\u5feb\u901f\u5f00\u5c55\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u5feb\u901f\u589e\u6b96\u53ca\u80c3\u817a\u764c\u8868\u578b\uff0c\u4fdd\u969c\u4e0e\u5176\u4ed6\u6d88\u5316\u9053\u80bf\u7624\u7ec6\u80de\u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CRL-1739\uff0cBCRC; 60102\uff0cBCRJ; 0311\uff1b\u4e2d\u56fd\u5178\u578b\u57f9\u517b\u7269\u4fdd\u85cf\u4e2d\u5fc3\u7ec6\u80de\u5e93\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\uff09\u3001\u5730\u533a\u6027\u673a\u6784\uff08BCRC\u3001BCRJ\uff09\u4e0e\u56fd\u5185\u673a\u6784\u56db\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u6781\u5f3a\uff0c\u80c3\u817a\u764c\u7279\u6027\u53ca\u751f\u7269\u5b66\u8868\u578b\u7ecf\u8fc7\u591a\u91cd\u9a8c\u8bc1\uff0c\u4e0e TE-1\uff08\u56fd\u5185\u72ec\u5bb6\u4fdd\u85cf\uff09\u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u8de8\u5730\u57df\u3001\u8de8\u56fd\u5bb6\u79d1\u7814\u534f\u4f5c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a90% F12K+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528 F12K \u57f9\u517b\u57fa\uff0c\u533a\u522b\u4e8e TE-1\u3001Eca-109 \u7684 RPMI-1640\u3001WPMY-1 \u7684 DMEM\uff1bF12K \u542b\u4e30\u5bcc\u5fae\u91cf\u5143\u7d20\uff08\u5982\u950c\u3001\u7852\uff09\u53ca\u751f\u957f\u56e0\u5b50\uff0c\u9002\u914d\u80c3\u817a\u764c\u7ec6\u80de\u5feb\u901f\u4ee3\u8c22\u9700\u6c42\uff0c\u7ef4\u6301\u5176\u817a\u7ba1\u6837\u5f62\u6001\u53ca\u9ecf\u6db2\u5206\u6ccc\u529f\u80fd\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u80c3\u9ecf\u819c\u523a\u6fc0\u56e0\u5b50\u5f71\u54cd\u7ec6\u80de\u817a\u764c\u8868\u578b\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u589e\u6b96\u901f\u5ea6\u53ca\u81f4\u7624\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 75%-80%\uff08\u9ad8\u4e8e\u5e38\u89c4\u7ec6\u80de\uff09\uff0c\u51cf\u5c11\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u7684\u6e17\u900f\u538b\u5347\u9ad8\uff0c\u907f\u514d\u52a0\u91cd\u7ec6\u80de\u4ee3\u8c22\u8d1f\u62c5\uff1b\u57f9\u517b\u7bb1\u9700\u8fdc\u79bb\u9891\u7e41\u5f00\u5173\u533a\u57df\uff0c\u9632\u6b62\u6e29\u5ea6\u6ce2\u52a8\u5f71\u54cd\u7ec6\u80de\u8d34\u58c1\u4e0e\u589e\u6b96\uff1b\u4e0e TE-1\u3001Eca-109\u3001WPMY-1 \u7b49\u6240\u6709\u5df2\u68b3\u7406\u7ec6\u80de\u7684\u57f9\u517b\u6761\u4ef6\u4e00\u81f4\uff0c\u4ec5\u9700\u63d0\u5347\u751f\u7269\u5b89\u5168\u9632\u62a4\u7b49\u7ea7\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u77ed\u500d\u589e\u65f6\u95f4\u4e0e\u8d34\u58c1\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u63a5\u79cd\u540e 24 \u5c0f\u65f6\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 1-2 \u5206\u949f\uff08\u77ed\u4e8e TE-1 \u7684 2-3 \u5206\u949f\uff09\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7834\u574f\u817a\u7ba1\u6837\u7ed3\u6784\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e\u5e38\u89c4\u7ec6\u80de\uff09\uff0c\u63d0\u5347\u590d\u82cf\u540e\u7ec6\u80de\u5bc6\u5ea6\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 F12K \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\u81f3\u9884\u6e29\u57f9\u517b\u74f6\uff0c\u51cf\u5c11\u5bf9\u7ec6\u80de\u589e\u6b96\u8282\u594f\u7684\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u500d\u589e\u65f6\u95f4\uff1a~20-24 hours\uff08\u589e\u6b96\u901f\u5ea6\u6781\u5feb\uff0c\u662f\u5df2\u68b3\u7406\u6d88\u5316\u9053\u80bf\u7624\u7ec6\u80de\u4e2d\u6700\u5feb\u7684\uff08\u5feb\u4e8e Eca-109 \u7684 28 \u5c0f\u65f6\u3001TE-1 \u7684 60 \u5c0f\u65f6\uff09\uff0c\u5bf9\u6570\u751f\u957f\u671f\u4e3a\u63a5\u79cd\u540e 12-24 \u5c0f\u65f6\uff0c\u9700\u6bcf\u5929\u955c\u68c0 2 \u6b21\u7ec6\u80de\u5bc6\u5ea6\uff0c\u5f85\u5bc6\u5ea6\u8fbe 70%-80% \u65f6\u7acb\u5373\u4f20\u4ee3\uff08\u907f\u514d\u8fc7\u5ea6\u5bc6\u96c6\u5bfc\u81f4\u7ec6\u80de\u8131\u843d\uff09\uff1b\u5b9e\u9a8c\u8bbe\u8ba1\u9700\u9002\u914d\u5feb\u8282\u594f\uff0c\u5982\u836f\u7269\u5904\u7406\u5468\u671f\u5efa\u8bae 24-48 \u5c0f\u65f6\uff0c\u8bbe\u7f6e 0\u300112\u300124\u300148 \u5c0f\u65f6\u591a\u4e2a\u65f6\u95f4\u8282\u70b9\u76d1\u6d4b\u7ec6\u80de\u54cd\u5e94\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u81f4\u7624\u6027\uff1aYes, in athymic BALB\/c mice.\uff08\u660e\u786e\u7684\u88f8\u9f20\uff08\u65e0\u80f8\u817a BALB\/c \u5c0f\u9f20\uff09\u6210\u7624\u80fd\u529b\uff0c\u6210\u7624\u6548\u7387\u9ad8\u3001\u5468\u671f\u77ed\uff08\u7ed3\u5408 20-24 \u5c0f\u65f6\u500d\u589e\u65f6\u95f4\uff0c\u63a8\u6d4b\u76ae\u4e0b\u6210\u7624 1-2 \u5468\u53ef\u89c1\u660e\u663e\u80bf\u7624\uff09\uff1b\u5efa\u8bae\u63a5\u79cd\u5bc6\u5ea6\u4e3a 5\u00d710\u2075cells \/ \u53ea\uff08\u4f4e\u4e8e Eca-109 \u7684 1\u00d710\u2076cells \/ \u53ea\uff09\uff0c\u6210\u7624\u540e\u901a\u8fc7 HE \u67d3\u8272\u89c2\u5bdf\u817a\u7ba1\u6837\u80bf\u7624\u7ed3\u6784\uff0c\u9a8c\u8bc1\u4f53\u5185\u80c3\u817a\u764c\u8868\u578b\uff0c\u9002\u5408\u5f00\u5c55\u4f53\u5185\u836f\u7269 efficacy \u5feb\u901f\u8bc4\u4f30\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 F12K \u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001\u500d\u589e\u65f6\u95f4\u68c0\u6d4b\u3001\u690d\u677f\u7387\u9a8c\u8bc1\u53ca\u817a\u764c\u6807\u5fd7\u7269\u68c0\u6d4b\uff0c\u786e\u4fdd\u6838\u5fc3\u7279\u6027\u7a33\u5b9a\uff1b\u4e0b\u5355\u540e 24 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff08\u56e0\u589e\u6b96\u5feb\u9700\u5feb\u901f\u5468\u8f6c\uff09\uff0c\u53ef\u4e0e TE-1\u3001Eca-109 \u540c\u6b65\u4e0b\u5355\uff0c\u4fdd\u969c \u201c\u6d88\u5316\u9053\u8de8\u5668\u5b98\u201d \u5e73\u884c\u5b9e\u9a8c\u7684\u65f6\u95f4\u4e00\u81f4\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u907f\u514d\u4efb\u4f55\u98a0\u7c38\uff0c\u51cf\u5c11\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165\u751f\u7269\u5b89\u5168\u4e8c\u7ea7\u5b9e\u9a8c\u5ba4\u7684 37\u2103\u57f9\u517b\u7bb1\uff0c12 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u60c5\u51b5\uff0c\u56e0\u589e\u6b96\u5feb\uff0c24 \u5c0f\u65f6\u5185\u9700\u5224\u65ad\u662f\u5426\u4f20\u4ee3\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff09 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