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\u5bf9\u6bd4\u5b9e\u9a8c\uff0c\u9a8c\u8bc1\u5e74\u9f84\u5bf9\u5370\u6212\u7ec6\u80de\u764c\u6cbb\u7597\u54cd\u5e94\u7684\u5f71\u54cd\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin\uff1aX\uff08\u660e\u786e\u5973\u6027\u6027\u522b\uff0c\u4e0e MKN-1 \u7684 Amelogenin\uff1aX \u4e00\u81f4\uff0c\u9a8c\u8bc1\u7ec6\u80de\u6027\u522b\u4e00\u81f4\u6027\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff0c\u5f3a\u5316 \u201c\u5973\u6027\u5370\u6212\u7ec6\u80de\u764c\u201d \u7684\u4e9a\u578b\u8eab\u4efd\uff09; CSF1PO\uff1a12\uff1bD13S317\uff1a8\uff0c11\uff1bD16S539\uff1a10\uff1bD18S51\uff1a16\uff1bD19S433\uff1a14\uff0c16.2\uff1bD21S11\uff1a31\uff1bD2S1338\uff1aOL\uff08\u7b49\u4f4d\u57fa\u56e0\u7f3a\u5931\uff0c\u5370\u6212\u7ec6\u80de\u764c\u67d3\u8272\u4f53\u5f02\u5e38\u7279\u5f81\uff09\uff1bD3S1358\uff1a15\uff0c16\uff1bD5S818\uff1a10\uff0c11\uff1bD7S820\uff1a10\uff0c11\uff1bD8S1179\uff1a13\uff0c17\uff1bFGA\uff1a19\uff0c24\uff1bTH01\uff1a7\uff1bTPOX\uff1a8\uff1bvWA\uff1a19\uff1b\uff08STR \u4f4d\u70b9\u542b\u7b49\u4f4d\u57fa\u56e0\u7f3a\u5931\uff08D2S1338\uff1aOL\uff09\uff0c\u4e0e MKN-1 \u7684\u67d3\u8272\u4f53\u5f02\u5e38\u7279\u5f81\u76f8\u4f3c\uff0c\u53ef\u901a\u8fc7\u56fe\u8c31\u4e0e\u4fdd\u85cf\u673a\u6784\u6570\u636e\u6bd4\u5bf9\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\uff0c\u540c\u65f6\u4e0e MGC-803\u3001HGC-27 \u7684 STR \u56fe\u8c31\u5dee\u5f02\u663e\u8457\uff0c\u907f\u514d\u7ec6\u80de\u6df7\u6dc6\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f7f\u7528\u6743\u9650\uff1aA \u7c7b\uff08\u4e0e MKN-1\uff08\u672a\u660e\u786e\u6743\u9650\uff09\u3001LNCaP \u7b49\u7ec6\u80de\u6743\u9650\u4e00\u81f4\uff0c\u5c5e\u4e8e\u5f00\u653e\u83b7\u53d6\u7684\u79d1\u7814\u7528\u7ec6\u80de\u682a\uff0c\u65e0\u9700\u7279\u6b8a\u7533\u8bf7\u5373\u53ef\u83b7\u53d6\uff0c\u4fbf\u4e8e\u5404\u7c7b\u79d1\u7814\u673a\u6784\u5f00\u5c55\u5370\u6212\u7ec6\u80de\u764c\u5e74\u9f84\u68af\u5ea6\u3001\u6df7\u5408\u751f\u957f\u76f8\u5173\u7814\u7a76\uff0c\u5c24\u5176\u9002\u5408\u56fd\u5185\u5b9e\u9a8c\u5ba4\u5f00\u5c55 \u201cMKN \u7cfb\u5217\u201d \u7ec6\u80de\u534f\u4f5c\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u9700\u5305\u542b\u8d34\u58c1\u7ec6\u80de\u4e0e\u60ac\u6d6e\u7ec6\u80de\u56e2\uff08\u5439\u6563\u540e\u8ba1\u6570\uff09\uff1b\u51bb\u5b58\u7ba1\u542b 10% DMSO \u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226575%\uff08\u4f4e\u4e8e MKN-1 \u7684 80%\uff0c\u56e0\u6df7\u5408\u751f\u957f\u5bf9\u51bb\u5b58\u66f4\u654f\u611f\uff09\uff0c\u4e14\u5370\u6212\u5f62\u6001\u3001\u6df7\u5408\u751f\u957f\u6bd4\u4f8b\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u6807\u6ce8 \u201c\u542b\u5c11\u91cf\u60ac\u6d6e\u7ec6\u80de\u201d\uff0c\u63d0\u9192\u7528\u6237\u6536\u96c6\u4e0a\u6e05\u7ec6\u80de\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR 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MKN-1\uff08\u672a\u660e\u786e\u4fdd\u85cf\uff09\u3001MGC-803\uff08\u591a\u673a\u6784\u4fdd\u85cf\uff09\u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u79d1\u7814\u673a\u6784\u5f00\u5c55\u5370\u6212\u7ec6\u80de\u764c\u672c\u571f\u5316\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1aRPMI-1640\uff0b10% FBS\uff0b1% P\/S\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u4e0e MKN-1 \u7684 RPMI-1640 \u914d\u65b9\u4e00\u81f4\uff0c\u533a\u522b\u4e8e HGC-27 \u7684 DMEM\uff1bRPMI-1640 \u542b\u4e30\u5bcc\u6c28\u57fa\u9178\uff0c\u9002\u914d\u5370\u6212\u7ec6\u80de\u7c98\u6db2\u5408\u6210\u9700\u6c42\uff0c\u540c\u65f6\u652f\u6301\u60ac\u6d6e\u7ec6\u80de\u56e2\u751f\u957f\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u9ecf\u9644\u56e0\u5b50\u6539\u53d8\u6df7\u5408\u751f\u957f\u6bd4\u4f8b\uff1b\u2462P\/S \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u60ac\u6d6e\u7ec6\u80de\u6d3b\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 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