{"id":179,"date":"2025-08-23T21:02:02","date_gmt":"2025-08-23T13:02:02","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=179"},"modified":"2025-08-23T21:02:02","modified_gmt":"2025-08-23T13:02:02","slug":"colo205%e4%ba%ba%e7%bb%93%e8%82%a0%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/colo205%e4%ba%ba%e7%bb%93%e8%82%a0%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"COLO205\u4eba\u7ed3\u80a0\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u540d\u79f0\uff1aCOLO205 \u4eba\u7ed3\u80a0\u764c\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff08\u4e0e Caco-2\uff08\u539f\u53d1\u7ed3\u80a0\u764c\uff09\u540c\u5c5e\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\uff0c\u533a\u522b\u4e8e SNU-216\uff08\u80c3\u764c\uff09\u3001GES-1\uff08\u6b63\u5e38\u80c3\u9ecf\u819c\uff09\uff0c\u6838\u5fc3\u7279\u8272\u4e3a \u201c\u8179\u6c34\u8f6c\u79fb\u6765\u6e90 + \u534a\u8d34\u534a\u60ac\u6df7\u5408\u751f\u957f + \u77ed\u500d\u589e\u65f6\u95f4\u201d\uff0c\u662f\u7ed3\u76f4\u80a0\u764c\u8f6c\u79fb\u673a\u5236\u3001\u5316\u7597\u8010\u836f\uff08\u7ecf 5 &#8211; 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\u8f6c\u79fb\u201d \u72b6\u6001\u7684\u5206\u5b50\u7279\u5f81\u53d8\u5316\uff1b\u2462\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u7ed3\u76f4\u80a0\u764c\u8179\u6c34\u8f6c\u79fb\u673a\u5236\u7814\u7a76\uff08\u5982\u8179\u819c\u4fb5\u88ad\u3001\u514d\u75ab\u9003\u9038\uff09\u3001\u6c1f\u5c3f\u5627\u5576\u7c7b\u836f\u7269\u8010\u836f\u7814\u7a76\uff08\u5982\u8010\u836f\u9006\u8f6c\u5242\u7b5b\u9009\uff09\u3001\u201c\u539f\u53d1 &#8211; \u8f6c\u79fb\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff0c\u540c\u65f6\u53ef\u4e0e Caco-2 \u642d\u914d\u5f00\u5c55\u836f\u7269\u7b5b\u9009\uff0c\u9a8c\u8bc1\u836f\u7269\u5bf9\u539f\u53d1\u4e0e\u8f6c\u79fb\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u7684\u7597\u6548\u5dee\u5f02\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e Caco-2\u3001SNU-216\u3001GES-1 \u7684\u5b89\u5168\u7b49\u7ea7\u4e00\u81f4\uff0c\u4f4e\u4e8e HGC-27\uff08\u7b49\u7ea7 2\uff09\u3001WPMY-1\uff08\u7b49\u7ea7 2\uff09\uff0c\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u4fbf\u4e8e\u5b9e\u9a8c\u5ba4\u7edf\u4e00\u7ba1\u7406\u4e0d\u540c\u5b89\u5168\u7b49\u7ea7\u7684\u6d88\u5316\u9053\u7ec6\u80de\uff0c\u964d\u4f4e\u5b9e\u9a8c\u64cd\u4f5c\u95e8\u69db\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u9700\u5305\u542b\u8d34\u58c1\u7ec6\u80de\u4e0e\u60ac\u6d6e\u7ec6\u80de\uff08\u5439\u6563\u540e\u8ba1\u6570\uff09\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226585%\uff08\u56e0\u77ed\u500d\u589e\u65f6\u95f4\uff0c\u590d\u82cf\u540e 24 \u5c0f\u65f6\u5373\u53ef\u5feb\u901f\u589e\u6b96\uff09\uff0c\u4e14\u6df7\u5408\u751f\u957f\u6bd4\u4f8b\u3001\u8010\u836f\u7279\u6027\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u6807\u6ce8 \u201c\u542b\u60ac\u6d6e\u7ec6\u80de\uff08\u6b63\u5e38\uff09\u201d\uff0c\u63d0\u9192\u7528\u6237\u6536\u96c6\u4e0a\u6e05\u7ec6\u80de\uff0c\u907f\u514d\u6d3b\u6027\u7ec6\u80de\u6d41\u5931\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u6df7\u5408\u751f\u957f\u5e73\u8861\u3001\u589e\u6b96\u901f\u5ea6\u53ca\u8010\u836f\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u4e0e Caco-2 \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CCL-222\uff0cBCRC; 60054\uff0cECACC; 87061208\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\u3001ECACC\uff09\u4e0e\u5730\u533a\u6027\u673a\u6784\uff08BCRC\uff09\u591a\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u8179\u6c34\u8f6c\u79fb\u7279\u6027\u53ca\u8010\u836f\u8868\u578b\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4e0e Caco-2\uff08\u591a\u673a\u6784\u4fdd\u85cf\uff09\u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u673a\u6784\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a90% RPMI-1640+10% FBS+1% PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528 RPMI-1640 \u57f9\u517b\u57fa\uff0c\u533a\u522b\u4e8e Caco-2 \u7684 MEM\uff08\u542b NEAA\uff09\uff0c\u4e0e SNU-216\u3001MKN-45 \u7684 RPMI-1640 \u914d\u65b9\u4e00\u81f4\uff1bRPMI-1640 \u542b\u4e30\u5bcc\u6c28\u57fa\u9178\u53ca\u8461\u8404\u7cd6\uff0c\u9002\u914d\u8179\u6c34\u8f6c\u79fb\u7ec6\u80de\u7684\u5feb\u901f\u4ee3\u8c22\u9700\u6c42\uff0c\u652f\u6301\u5176\u77ed\u500d\u589e\u65f6\u95f4\u53ca\u6df7\u5408\u751f\u957f\u6a21\u5f0f\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u9ecf\u9644\u56e0\u5b50\u6539\u53d8\u6df7\u5408\u751f\u957f\u6bd4\u4f8b\uff08\u5982\u51cf\u5c11\u60ac\u6d6e\u7ec6\u80de\uff09\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u8010\u836f\u6027\u53ca\u8f6c\u79fb\u7279\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u60ac\u6d6e\u7ec6\u80de\u56e2\u5e72\u6db8\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u8d34\u58c1\u7ec6\u80de\u8131\u843d\u52a0\u5267\uff1b\u4e0e Caco-2\u3001SNU-216\u3001GES-1 \u7b49\u6240\u6709\u5df2\u68b3\u7406\u7ec6\u80de\u7684\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u4ec5\u9700\u9002\u914d\u6df7\u5408\u751f\u957f\u7279\u6027\u8c03\u6574\u64cd\u4f5c\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u6df7\u5408\u751f\u957f\u4e0e\u77ed\u500d\u589e\u7279\u6027\uff1a\u2460\u540c\u65f6\u6536\u96c6\u8d34\u58c1\u7ec6\u80de\uff080.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 1.5-2.5 \u5206\u949f\uff0c\u77ed\u4e8e Caco-2 \u7684 3-4 \u5206\u949f\uff09\u4e0e\u60ac\u6d6e\u7ec6\u80de\uff0c\u5408\u5e76\u540e\u8f7b\u67d4\u5439\u6563\u5927\u56e2\u5757\uff08\u4fdd\u7559\u5c0f\u56e2\u5757\uff0c\u907f\u514d\u5355\u7ec6\u80de\u5316\u5bfc\u81f4\u8010\u836f\u6027\u4e22\u5931\uff09\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e\u5e38\u89c4\u7ec6\u80de\uff0c\u8865\u507f\u6df7\u5408\u751f\u957f\u7684\u7ec6\u80de\u635f\u5931\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 RPMI-1640 \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\uff0c\u907f\u514d DMSO \u5bf9\u8010\u836f\u76f8\u5173\u86cb\u767d\u8868\u8fbe\u7684\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u500d\u589e\u65f6\u95f4\uff1a~25-30 hours\uff08\u589e\u6b96\u901f\u5ea6\u6781\u5feb\uff0c\u662f\u5df2\u68b3\u7406\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u4e2d\u6700\u5feb\u7684\uff08\u5feb\u4e8e Caco-2 \u7684 60-80 \u5c0f\u65f6\uff0c\u4e0e\u80c3\u764c\u7ec6\u80de Eca-109 \u7684 28 \u5c0f\u65f6\u76f8\u8fd1\uff09\uff0c\u5bf9\u6570\u751f\u957f\u671f\u4e3a\u63a5\u79cd\u540e 12-24 \u5c0f\u65f6\uff0c\u9700\u6bcf\u5929\u955c\u68c0 2 \u6b21\u7ec6\u80de\u5bc6\u5ea6\uff0c\u5f85\u8d34\u58c1\u7ec6\u80de\u8fbe 70% \u65f6\u7acb\u5373\u4f20\u4ee3\uff08\u907f\u514d\u8fc7\u5ea6\u5bc6\u96c6\u5bfc\u81f4\u60ac\u6d6e\u7ec6\u80de\u6bd4\u4f8b\u5f02\u5e38\u5347\u9ad8\uff09\uff1b\u5b9e\u9a8c\u8bbe\u8ba1\u9700\u9002\u914d\u5feb\u8282\u594f\uff0c\u5982\u836f\u7269\u5904\u7406\u65f6\u95f4\u5efa\u8bae 24-48 \u5c0f\u65f6\uff0c\u8010\u836f\u5b9e\u9a8c\u9700\u7f29\u77ed\u836f\u7269\u4f5c\u7528\u5468\u671f\uff0c\u907f\u514d\u7ec6\u80de\u8fc7\u5ea6\u589e\u6b96\u5f71\u54cd\u7ed3\u679c\u5224\u65ad\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u81f4\u7624\u6027\uff1aYes, in nude mice (Tumors developed within 21 days at 100% frequency (5\/5) in nude mice inoculated subcutaneously with 1\u00d710^7 cells).\uff08\u660e\u786e\u7684\u88f8\u9f20\u9ad8\u6210\u7624\u80fd\u529b\uff0c\u6210\u7624\u5468\u671f\u77ed\uff0821 \u5929\u5185\uff09\u3001\u6210\u7624\u7387 100%\uff0c\u4e14\u63a5\u79cd\u5bc6\u5ea6\uff081\u00d710^7cells \/ \u53ea\uff09\u9ad8\u4e8e Caco-2\uff082\u00d710^6cells \/ \u53ea\uff09\uff0c\u4f53\u73b0\u8f6c\u79fb\u764c\u7ec6\u80de\u66f4\u5f3a\u7684\u4f53\u5185\u589e\u6b96\u80fd\u529b\uff1b\u5efa\u8bae\u540c\u65f6\u6784\u5efa\u76ae\u4e0b\u6210\u7624\u4e0e\u8179\u8154\u6210\u7624\u6a21\u578b\uff08\u6a21\u62df\u8179\u6c34\u8f6c\u79fb\uff09\uff0c\u8179\u8154\u63a5\u79cd\u5bc6\u5ea6 5\u00d710^6cells \/ \u53ea\uff0c21 \u5929\u540e\u89c2\u5bdf\u8179\u6c34\u5f62\u6210\u53ca\u8179\u8154\u5185\u80bf\u7624\u5b9a\u690d\u60c5\u51b5\uff0c\u66f4\u8d34\u5408\u4e34\u5e8a\u8179\u6c34\u8f6c\u79fb\u573a\u666f\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff1acarcinoembryonic antigen (CEA) 1.5 to 4.1 ng\/10^6 cells cells\/10 days\uff08\u7ed3\u76f4\u80a0\u764c\u6807\u5fd7\u7269\uff0c\u8868\u8fbe\u91cf\u660e\u786e\uff0c\u53ef\u901a\u8fc7 ELISA \u6cd5\u5b9a\u91cf\u68c0\u6d4b\uff0c\u4e0e Caco-2\uff08CEA \u4f4e\u8868\u8fbe\uff09\u5bf9\u6bd4\uff0c\u9a8c\u8bc1 \u201c\u8f6c\u79fb\u7076 CEA \u9ad8\u8868\u8fbe\u201d \u7684\u4e34\u5e8a\u7279\u5f81\uff09; keratin\uff08\u4e0a\u76ae\u7ec6\u80de\u6807\u5fd7\u7269\uff0c\u514d\u75ab\u8fc7\u6c27\u5316\u7269\u9176\u67d3\u8272\u9633\u6027\uff0c\u9a8c\u8bc1\u7ec6\u80de\u4e0a\u76ae\u5c5e\u6027\uff09; interleukin 10 (IL-10, interleukin-10)\uff08\u514d\u75ab\u6291\u5236\u56e0\u5b50\uff0c\u53ef\u80fd\u901a\u8fc7\u6291\u5236\u514d\u75ab\u7ec6\u80de\u6d3b\u6027\u4fc3\u8fdb\u8179\u6c34\u8f6c\u79fb\uff0c\u53ef\u7528\u4e8e\u7ed3\u76f4\u80a0\u764c\u514d\u75ab\u9003\u9038\u673a\u5236\u7814\u7a76\uff09\uff08\u57fa\u56e0\u8868\u8fbe\u4e0e\u8179\u6c34\u8f6c\u79fb\u3001\u514d\u75ab\u9003\u9038\u9ad8\u5ea6\u5173\u8054\uff0c\u53ef\u901a\u8fc7 RT-PCR \u6216 Western Blot \u68c0\u6d4b\u5176\u8868\u8fbe\u6c34\u5e73\uff0c\u5206\u6790\u8010\u836f\u5904\u7406\uff08\u5982 5 &#8211; \u6c1f\u5c3f\u5627\u5576\uff09\u5bf9\u57fa\u56e0\u8868\u8fbe\u7684\u5f71\u54cd\uff0c\u63a2\u7d22\u8010\u836f\u4e0e\u8f6c\u79fb\u7684\u5173\u8054\u673a\u5236\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a2 \u5468\u5de6\u53f3\uff08\u56e0\u9700\u7ef4\u6301\u6df7\u5408\u751f\u957f\u7279\u6027\u53ca\u8010\u836f\u8868\u578b\u7a33\u5b9a\u6027\uff0c\u5e93\u5b58\u7ec6\u80de\u9700\u7ecf\u8fc7\u8010\u836f\u6027\u9a8c\u8bc1\uff08\u5982 5 &#8211; \u6c1f\u5c3f\u5627\u5576 IC50 \u68c0\u6d4b\uff09\uff0c\u5236\u5907\u5468\u671f\u957f\u4e8e Caco-2 \u7684 1 \u5468\uff1b\u4e0b\u5355\u540e\u9700\u63d0\u524d\u6c9f\u901a\u5b9e\u9a8c\u9700\u6c42\uff0c72 \u5c0f\u65f6\u5185\u542f\u52a8\u7ec6\u80de\u51c6\u5907\uff0c\u786e\u4fdd 2 \u5468\u5185\u53d1\u8d27\uff0c\u53ef\u4e0e Caco-2 \u9519\u5cf0\u4e0b\u5355\uff0c\u4fdd\u969c \u201c\u539f\u53d1 &#8211; \u8f6c\u79fb\u201d \u5b9e\u9a8c\u7684\u65f6\u95f4\u8854\u63a5\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u907f\u514d\u5267\u70c8\u98a0\u7c38\uff0c\u51cf\u5c11\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165 37\u2103\u57f9\u517b\u7bb1\uff0c12 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u72b6\u6001\uff0c\u56e0\u589e\u6b96\u5feb\uff0c24 \u5c0f\u65f6\u5185\u9700\u5224\u65ad\u662f\u5426\u4f20\u4ee3\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u6668\u8fbe\u670d\u52a1\uff0c\u6700\u5927\u9650\u5ea6\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca\u8010\u836f\u7279\u6027\u6539\u53d8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e Caco-2\u3001SNU-216 \u7b49\u7ec6\u80de\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff1b\u56e0\u5177\u5907\u5316\u7597\u8010\u836f\u80cc\u666f\uff0c\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u9700\u59a5\u5584\u5904\u7406\u542b\u836f\u7269\u7684\u57f9\u517b\u57fa\uff0c\u907f\u514d\u73af\u5883\u6c61\u67d3\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460\u6df7\u5408\u751f\u957f\u7ef4\u62a4\uff1a\u4f20\u4ee3\u65f6\u7528\u79fb\u6db2\u7ba1\u8f7b\u8f7b\u5438\u53d6\u4e0a\u6e05\uff08\u4fdd\u7559\u5e95\u90e8 1mL \u65e7\u57f9\u517b\u57fa\uff09\uff0c\u6536\u96c6\u60ac\u6d6e\u7ec6\u80de\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u540e\u4e0e\u8d34\u58c1\u6d88\u5316\u7ec6\u80de\u5408\u5e76\u4f20\u4ee3\uff1b\u82e5\u60ac\u6d6e\u7ec6\u80de\u6bd4\u4f8b\u8d85\u8fc7 40%\uff0c\u9700\u68c0\u67e5\u57f9\u517b\u7bb1\u6e29\u5ea6\uff08\u6ce2\u52a8\u9700\uff1c\u00b10.5\u2103\uff09\u53ca\u57f9\u517b\u57fa\u6279\u6b21\uff0c\u6392\u9664\u73af\u5883\u56e0\u7d20\u5bfc\u81f4\u7684\u8d34\u58c1\u80fd\u529b\u4e0b\u964d\uff1b\u6362\u6db2\u9891\u7387\u5efa\u8bae 1-2 \u5929 1 \u6b21\uff0c\u6362\u6db2\u65f6\u4fdd\u7559 1\/4 \u65e7\u57f9\u517b\u57fa\uff08\u542b\u60ac\u6d6e\u7ec6\u80de\u751f\u957f\u6240\u9700\u7684 IL-10 \u7b49\u56e0\u5b50\uff09\uff1b\u2461\u8010\u836f\u5b9e\u9a8c\u8bbe\u8ba1\uff1a\u5f00\u5c55 5 &#8211; \u6c1f\u5c3f\u5627\u5576\u8010\u836f\u5b9e\u9a8c\u65f6\uff0c\u8bbe\u7f6e Caco-2\uff08\u654f\u611f\u5bf9\u7167\uff09\u3001COLO205\uff08\u8010\u836f\u6a21\u578b\uff09\uff0c\u836f\u7269\u6d53\u5ea6\u68af\u5ea6\u5efa\u8bae 0.1-100\u03bcM\uff0c\u5904\u7406 48 \u5c0f\u65f6\u540e\u901a\u8fc7 CCK-8 \u6cd5\u68c0\u6d4b IC50 \u503c\uff0cCOLO205 \u7684 IC50 \u5e94\u663e\u8457\u9ad8\u4e8e Caco-2\uff08\u901a\u5e38\u4e3a 2-5 \u500d\uff09\uff1b\u53ef\u901a\u8fc7 Western Blot \u68c0\u6d4b\u8010\u836f\u76f8\u5173\u86cb\u767d\uff08\u5982 ABC \u8f6c\u8fd0\u86cb\u767d\u3001DNA \u4fee\u590d\u9176\uff09\u8868\u8fbe\uff0c\u9a8c\u8bc1\u8010\u836f\u673a\u5236\uff1b\u2462\u201c\u539f\u53d1 &#8211; \u8f6c\u79fb\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff1a\u642d\u914d Caco-2 \u5f00\u5c55\u5b9e\u9a8c\u65f6\uff0c\u53ef\uff1aa. \u68c0\u6d4b\u8f6c\u79fb\u76f8\u5173\u57fa\u56e0\uff08\u5982 MMP9\u3001CXCR4\uff0cCOLO205 \u9ad8\u8868\u8fbe\uff09\u4e0e\u5206\u5316\u76f8\u5173\u57fa\u56e0\uff08\u5982\u7ef4\u7532\u9178\u7ed3\u5408\u86cb\u767d\uff0cCaco-2 \u9ad8\u8868\u8fbe\uff09\u7684\u8868\u8fbe\u5dee\u5f02\uff0c\u5206\u6790 \u201c\u539f\u53d1 &#8211; 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