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\u7f9f\u8272\u80fa\u3001\u53bb\u7532\u80be\u4e0a\u817a\u7d20\u3001\u80be\u4e0a\u817a\u7d20\u3001ACTH\uff08\u4fc3\u80be\u4e0a\u817a\u76ae\u8d28\u6fc0\u7d20\uff09\u548c\u7532\u72b6\u65c1\u817a\u7d20\uff0c\u89d2\u86cb\u767d\u3001\u6ce2\u5f62\u86cb\u767d\u5f31\u9633\u6027\uff0c\u6838\u5fc3\u7279\u6027\u53ca\u5e94\u7528\u5982\u4e0b\uff1a\u2460\u6fc0\u7d20\u5206\u6ccc\u7279\u8272\uff1a\u4f5c\u4e3a\u5c11\u6570\u5177\u5907\u795e\u7ecf\u5185\u5206\u6ccc\u6837\u8868\u578b\u7684\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\uff0c\u6fc0\u7d20\u5206\u6ccc\u529f\u80fd\u6a21\u62df\u4e34\u5e8a \u201c\u795e\u7ecf\u5185\u5206\u6ccc\u578b\u7ed3\u76f4\u80a0\u764c\u201d\uff08\u5360\u7ed3\u76f4\u80a0\u764c 1%-2%\uff0c\u9884\u540e\u5dee\uff09\uff0c\u53ef\u7528\u4e8e\u80bf\u7624 &#8211; 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\u4e0e\u8f6c\u79fb\u9636\u6bb5\u7684\u5173\u8054\u7814\u7a76\uff1b\u2462\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u7ed3\u76f4\u80a0\u764c\u5c40\u90e8\u8f6c\u79fb\u673a\u5236\uff08\u5982\u6dcb\u5df4\u7ed3\u4fb5\u88ad\uff09\u3001\u795e\u7ecf\u5185\u5206\u6ccc\u6837\u8868\u578b\u8c03\u63a7\u3001\u6fc0\u7d20\u9776\u5411\u6cbb\u7597\u7b5b\u9009\uff0c\u540c\u65f6\u53ef\u4e0e COLO205 \u642d\u914d\u5f00\u5c55 \u201c\u5c40\u90e8\u8f6c\u79fb &#8211; \u8fdc\u5904\u8f6c\u79fb\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff0c\u9a8c\u8bc1\u836f\u7269\u5bf9\u4e0d\u540c\u8f6c\u79fb\u9636\u6bb5\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u7684\u7597\u6548\u5dee\u5f02\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin\uff1aX\uff08\u660e\u786e\u5973\u6027\u6027\u522b\uff0c\u4e0e\u6027\u522b\u6807\u6ce8\u4e00\u81f4\uff0c\u9a8c\u8bc1\u7ec6\u80de\u8eab\u4efd\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff0c\u4e0e MKN-45\uff08Amelogenin\uff1aX\uff09\u5171\u540c\u786e\u8ba4\u5973\u6027\u6d88\u5316\u9053\u80bf\u7624\u7ec6\u80de\u8eab\u4efd\uff09; CSF1PO\uff1a11\uff1bD13S317\uff1a11\uff1bD16S539\uff1a11\uff0c12\uff1bD18S51\uff1a15\uff1bD19S433\uff1a16.2\uff1bD21S11\uff1a33.2\uff1bD2S1338\uff1a18\uff0c25\uff1bD3S1358\uff1a17\uff1bD5S818\uff1a12\uff1bD7S820\uff1a9\uff0c12\uff1bD8S1179\uff1a13\uff1bFGA\uff1a20\uff1bTH01\uff1a9\uff1bTPOX\uff1a8\uff0c9\uff1bvWA\uff1a15\uff0c18\uff1b\uff08STR \u4f4d\u70b9\u4ee5\u7eaf\u5408\u5b50\u548c\u53cc\u7b49\u4f4d\u57fa\u56e0\u4e3a\u4e3b\uff0c\u65e0\u591a\u7b49\u4f4d\u57fa\u56e0\uff08\u533a\u522b\u4e8e COLO205 \u7684\u591a\u7b49\u4f4d\u57fa\u56e0\uff09\uff0c\u4f53\u73b0\u8f83\u4f4e\u7684\u67d3\u8272\u4f53\u4e0d\u7a33\u5b9a\u6027\uff0c\u4e0e 49~52 \u6761\u67d3\u8272\u4f53\u7684\u4f4e\u5f02\u500d\u4f53\u7279\u5f81\u4e00\u81f4\uff1b\u53ef\u901a\u8fc7\u56fe\u8c31\u4e0e ATCC CCL-220 \u6570\u636e\u6bd4\u5bf9\uff0c\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\u53ca Dukes \u6c0f C \u578b\u7279\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e COLO205\u3001Caco-2\u3001GES-1 \u7684\u5b89\u5168\u7b49\u7ea7\u4e00\u81f4\uff0c\u4f4e\u4e8e HGC-27\uff08\u7b49\u7ea7 2\uff09\u3001WPMY-1\uff08\u7b49\u7ea7 2\uff09\uff0c\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u4fbf\u4e8e\u5b9e\u9a8c\u5ba4\u7edf\u4e00\u7ba1\u7406\u4e0d\u540c\u5b89\u5168\u7b49\u7ea7\u7684\u6d88\u5316\u9053\u7ec6\u80de\uff0c\u964d\u4f4e\u5b9e\u9a8c\u64cd\u4f5c\u95e8\u69db\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u9700\u5305\u542b\u8d34\u58c1\u7ec6\u80de\u4e0e\u60ac\u6d6e\u7ec6\u80de\uff08\u65e0\u9700\u5439\u6563\uff0c\u76f4\u63a5\u8ba1\u6570\u5706\u5f62\u7ec6\u80de\u56e2\uff09\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226580%\uff08\u60ac\u6d6e\u7ec6\u80de\u590d\u82cf\u5b58\u6d3b\u7387\u9ad8\u4e8e\u8d34\u58c1\u7ec6\u80de\uff09\uff0c\u4e14\u6fc0\u7d20\u5206\u6ccc\u91cf\u3001\u5706\u5f62\u6298\u5149\u5f62\u6001\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u6807\u6ce8 \u201c\u9ad8\u60ac\u6d6e\u7ec6\u80de\u6bd4\u4f8b\uff08\u6b63\u5e38\uff09\u201d\uff0c\u63d0\u9192\u7528\u6237\u6536\u96c6\u4e0a\u6e05\u7ec6\u80de\uff0c\u907f\u514d\u6d3b\u6027\u7ec6\u80de\u6d41\u5931\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u6df7\u5408\u751f\u957f\u5e73\u8861\u3001\u6fc0\u7d20\u5206\u6ccc\u53ca\u8f6c\u79fb\u76f8\u5173\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u4e0e COLO205 \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CCL-220\uff0cBCRC; 60108\uff0cECACC; 87061205\uff1b\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u57fa\u7840\u533b\u5b66\u7814\u7a76\u6240\u7ec6\u80de\u8d44\u6e90\u4e2d\u5fc3\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\u3001ECACC\uff09\u3001\u5730\u533a\u6027\u673a\u6784\uff08BCRC\uff09\u4e0e\u56fd\u5185\u673a\u6784\u56db\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u6781\u5f3a\uff0cDukes \u6c0f C \u578b\u7279\u6027\u53ca\u6fc0\u7d20\u5206\u6ccc\u8868\u578b\u7ecf\u8fc7\u591a\u91cd\u9a8c\u8bc1\uff0c\u4e0e COLO205\uff08\u591a\u673a\u6784\u4fdd\u85cf\uff09\u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u673a\u6784\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a90% RPMI-1640+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u4e0e COLO205 \u7684\u57f9\u517b\u57fa\u914d\u65b9\u5b8c\u5168\u4e00\u81f4\uff0c\u533a\u522b\u4e8e Caco-2 \u7684 MEM\uff08\u542b NEAA\uff09\uff1bRPMI-1640 \u542b\u4e30\u5bcc\u6c28\u57fa\u9178\u53ca\u8461\u8404\u7cd6\uff0c\u9002\u914d\u665a\u671f\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u7684\u4ee3\u8c22\u9700\u6c42\uff0c\u652f\u6301\u5176\u6fc0\u7d20\u5408\u6210\u53ca\u6df7\u5408\u751f\u957f\u6a21\u5f0f\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u6fc0\u7d20\u6210\u5206\u5e72\u6270\u7ec6\u80de\u81ea\u8eab\u5206\u6ccc\u529f\u80fd\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u8f6c\u79fb\u7279\u6027\u53ca\u6fc0\u7d20\u5206\u6ccc\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 75%-80%\uff08\u9ad8\u4e8e\u5e38\u89c4\u7ec6\u80de\uff09\uff0c\u51cf\u5c11\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u60ac\u6d6e\u7ec6\u80de\u56e2\u5e72\u6db8\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u8d34\u58c1\u7ec6\u80de\u8131\u843d\u52a0\u5267\uff1b\u4e0e COLO205\u3001Caco-2\u3001GES-1 \u7b49\u6240\u6709\u5df2\u68b3\u7406\u7ec6\u80de\u7684\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u4ec5\u9700\u9002\u914d\u9ad8\u60ac\u6d6e\u6bd4\u4f8b\u8c03\u6574\u64cd\u4f5c\uff08\u5982\u6362\u6db2\u65f6\u8f7b\u67d4\u5438\u53d6\u4e0a\u6e05\uff09\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u9ad8\u60ac\u6d6e\u4e0e\u6fc0\u7d20\u5206\u6ccc\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u60ac\u6d6e\u7ec6\u80de\u56e2\u5360\u6bd4 40% \u5de6\u53f3\uff09\uff0c\u8d34\u58c1\u7ec6\u80de\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 2-3 \u5206\u949f\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u540c\u65f6\u6536\u96c6\u4e0a\u6e05\u60ac\u6d6e\u7ec6\u80de\uff0c\u5408\u5e76\u540e\u8f7b\u67d4\u5439\u6253\uff08\u907f\u514d\u7834\u574f\u6fc0\u7d20\u5206\u6ccc\u76f8\u5173\u7ec6\u80de\u5668\uff09\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2.5\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e COLO205\uff0c\u8865\u507f\u60ac\u6d6e\u7ec6\u80de\u51bb\u5b58\u635f\u5931\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 40 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 3 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1.5 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 RPMI-1640 \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\uff0c\u907f\u514d DMSO \u5bf9\u6fc0\u7d20\u5206\u6ccc\u76f8\u5173\u86cb\u767d\uff08\u5982 ACTH \u5408\u6210\u9176\uff09\u7684\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u81f4\u7624\u6027\uff1aYes, in nude mice.\uff08\u660e\u786e\u7684\u88f8\u9f20\u6210\u7624\u80fd\u529b\uff0c\u6210\u7624\u75c5\u7406\u7c7b\u578b\u4e0e Dukes \u6c0f C \u578b\u4e00\u81f4\uff08\u5c40\u90e8\u6d78\u6da6\u6027\u751f\u957f\uff09\uff0c\u6210\u7624\u5468\u671f\u4e2d\u7b49\uff08\u7ed3\u5408\u589e\u6b96\u8282\u594f\uff0c\u63a8\u6d4b\u76ae\u4e0b\u6210\u7624 3-4 \u5468\u53ef\u89c1\u660e\u663e\u80bf\u7624\uff09\uff1b\u5efa\u8bae\u540c\u65f6\u6784\u5efa\u76ae\u4e0b\u6210\u7624\u4e0e\u6dcb\u5df4\u7ed3\u8f6c\u79fb\u6a21\u578b\uff08\u6a21\u62df\u4e34\u5e8a Dukes \u6c0f C \u578b\uff09\uff0c\u6dcb\u5df4\u7ed3\u63a5\u79cd\u5bc6\u5ea6 1\u00d710\u2076cells \/ \u53ea\uff0c28 \u5929\u540e\u89c2\u5bdf\u6dcb\u5df4\u7ed3\u5185\u80bf\u7624\u5b9a\u690d\u60c5\u51b5\uff0c\u66f4\u8d34\u5408\u665a\u671f\u5c40\u90e8\u8f6c\u79fb\u573a\u666f\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff1aserotonin\uff085 &#8211; \u7f9f\u8272\u80fa\uff0c\u795e\u7ecf\u9012\u8d28\uff0c\u53c2\u4e0e\u80bf\u7624\u7ec6\u80de\u589e\u6b96\u8c03\u63a7\uff09; norepinephrine\uff08\u53bb\u7532\u80be\u4e0a\u817a\u7d20\uff0c\u6fc0\u7d20\uff0c\u53ef\u80fd\u4fc3\u8fdb\u80bf\u7624\u4fb5\u88ad\uff09; epinephrine\uff08\u80be\u4e0a\u817a\u7d20\uff0c\u6fc0\u7d20\uff0c\u4e0e\u80bf\u7624\u8840\u7ba1\u751f\u6210\u76f8\u5173\uff09; adrenocorticotropic hormone (ACTH)\uff08\u4fc3\u80be\u4e0a\u817a\u76ae\u8d28\u6fc0\u7d20\uff0c\u53ef\u8bf1\u5bfc\u76ae\u8d28\u9187\u5206\u6ccc\uff0c\u53c2\u4e0e\u80bf\u7624\u514d\u75ab\u9003\u9038\uff09; parathyroid hormone\uff08\u7532\u72b6\u65c1\u817a\u7d20\uff0c\u53ef\u80fd\u5bfc\u81f4\u764c\u6027\u9ad8\u9499\u8840\u75c7\uff09\uff08\u57fa\u56e0\u8868\u8fbe\u4e0e\u795e\u7ecf\u5185\u5206\u6ccc\u6837\u8868\u578b\u9ad8\u5ea6\u5173\u8054\uff0c\u53ef\u901a\u8fc7 ELISA \u6cd5\u68c0\u6d4b\u57f9\u517b\u4e0a\u6e05\u4e2d\u6fc0\u7d20\u542b\u91cf\uff08\u5982 ACTH \u53c2\u8003\u503c\uff1a10-20pg\/mL\/10\u2076cells\uff09\uff0c\u4e0e COLO205\uff08\u65e0\u6fc0\u7d20\u5206\u6ccc\uff09\u5bf9\u6bd4\uff0c\u9a8c\u8bc1 \u201c\u665a\u671f\u7ed3\u76f4\u80a0\u764c\u6fc0\u7d20\u5206\u6ccc\u201d \u7684\u4e34\u5e8a\u7279\u5f81\uff1b\u6fc0\u7d20\u5206\u6ccc\u91cf\u53ef\u4f5c\u4e3a\u7ec6\u80de\u8868\u578b\u7a33\u5b9a\u6027\u7684\u5224\u65ad\u6307\u6807\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u67d3\u8272\u4f53\uff1a49~52\uff08\u67d3\u8272\u4f53\u6570\u76ee\u7565\u9ad8\u4e8e\u6b63\u5e38\u4e8c\u500d\u4f53\uff0846 \u6761\uff09\uff0c\u4e3a\u4f4e\u5f02\u500d\u4f53\uff0c\u4e0e COLO205 \u7684 85~100 \u6761\u9ad8\u5f02\u500d\u4f53\u5f62\u6210\u9c9c\u660e\u5bf9\u6bd4\uff0c\u4f53\u73b0\u4e0d\u540c\u8f6c\u79fb\u9636\u6bb5\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u7684\u9057\u4f20\u5dee\u5f02\uff1b\u67d3\u8272\u4f53\u6570\u76ee\u7a33\u5b9a\uff0c\u5b9e\u9a8c\u8bbe\u8ba1\u4e2d\u65e0\u9700\u8003\u8651\u9ad8\u5f02\u500d\u4f53\u5bfc\u81f4\u7684\u9776\u70b9\u8868\u8fbe\u6ce2\u52a8\uff0c\u9002\u5408\u5f00\u5c55\u7cbe\u51c6\u7684\u6fc0\u7d20\u8c03\u63a7\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 RPMI-1640 \u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001\u6fc0\u7d20\u5206\u6ccc\u68c0\u6d4b\uff08\u5982 5 &#8211; \u7f9f\u8272\u80fa ELISA\uff09\u53ca\u5706\u5f62\u6298\u5149\u5f62\u6001\u9a8c\u8bc1\uff0c\u786e\u4fdd\u6838\u5fc3\u7279\u6027\u7a33\u5b9a\uff1b\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u53ef\u4e0e COLO205 \u540c\u6b65\u4e0b\u5355\uff0c\u4fdd\u969c \u201c\u4e0d\u540c\u8f6c\u79fb\u9636\u6bb5\u201d \u5e73\u884c\u5b9e\u9a8c\u7684\u65f6\u95f4\u4e00\u81f4\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u907f\u514d\u4efb\u4f55\u98a0\u7c38\uff0c\u51cf\u5c11\u8d34\u58c1\u7ec6\u80de\u8131\u843d\u53ca\u60ac\u6d6e\u7ec6\u80de\u56e2\u7834\u788e\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165 37\u2103\u57f9\u517b\u7bb1\uff0c36 \u5c0f\u65f6\u5185\u4e0d\u6362\u6db2\uff0c\u5f85\u60ac\u6d6e\u7ec6\u80de\u56e2\u7a33\u5b9a\u540e\u518d\u64cd\u4f5c\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u6668\u8fbe\u670d\u52a1\uff0c\u6700\u5927\u9650\u5ea6\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca\u6fc0\u7d20\u5206\u6ccc\u7279\u6027\u6539\u53d8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e COLO205\u3001Caco-2 \u7b49\u7ec6\u80de\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff1b\u56e0\u5177\u5907\u6fc0\u7d20\u5206\u6ccc\u529f\u80fd\uff0c\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u9700\u59a5\u5584\u5904\u7406\u542b\u6fc0\u7d20\u7684\u57f9\u517b\u57fa\uff0c\u907f\u514d\u73af\u5883\u6c61\u67d3\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460\u6fc0\u7d20\u5206\u6ccc\u5b9e\u9a8c\u8bbe\u8ba1\uff1a\u68c0\u6d4b\u6fc0\u7d20\u5206\u6ccc\u65f6\uff0c\u9700\u6536\u96c6\u57f9\u517b 48 \u5c0f\u65f6\u7684\u4e0a\u6e05\uff08\u5bf9\u6570\u751f\u957f\u671f\uff09\uff0c3000rpm \u79bb\u5fc3 10 \u5206\u949f\u53bb\u9664\u7ec6\u80de\u788e\u7247\uff0c\u91c7\u7528\u7279\u5f02\u6027 ELISA \u8bd5\u5242\u76d2\uff08\u5982 ACTH \u8bd5\u5242\u76d2\u8d27\u53f7\uff1aE-EL-H0138\uff09\u68c0\u6d4b\uff0c\u8bbe\u7f6e COLO205\uff08\u9634\u6027\u5bf9\u7167\uff09\u3001\u6807\u51c6\u54c1\u68af\u5ea6\uff0c\u786e\u4fdd\u7ed3\u679c\u51c6\u786e\u6027\uff1b\u82e5\u9700\u8c03\u63a7\u6fc0\u7d20\u5206\u6ccc\uff0c\u53ef\u6dfb\u52a0\u6fc0\u7d20\u5408\u6210\u6291\u5236\u5242\uff08\u5982\u916e\u5eb7\u5511\u6291\u5236\u80be\u4e0a\u817a\u7d20\u5408\u6210\uff09\uff0c\u89c2\u5bdf\u7ec6\u80de\u589e\u6b96\u53ca\u8f6c\u79fb\u80fd\u529b\u53d8\u5316\uff1b\u2461\u6df7\u5408\u751f\u957f\u7ef4\u62a4\uff1a\u4f20\u4ee3\u65f6\u7528\u79fb\u6db2\u7ba1\u6cbf\u74f6\u58c1\u7f13\u6162\u5438\u53d6\u4e0a\u6e05\uff08\u4fdd\u7559\u5e95\u90e8 2mL \u65e7\u57f9\u517b\u57fa\uff09\uff0c\u6536\u96c6\u60ac\u6d6e\u7ec6\u80de\uff0c1000rpm \u79bb\u5fc3 5 \u5206\u949f\u540e\u4e0e\u8d34\u58c1\u6d88\u5316\u7ec6\u80de\u5408\u5e76\u4f20\u4ee3\uff1b\u82e5\u60ac\u6d6e\u7ec6\u80de\u6bd4\u4f8b\u4f4e\u4e8e 30%\uff0c\u9700\u68c0\u67e5\u57f9\u517b\u57fa pH \u503c\uff08\u5e94\u7ef4\u6301\u5728 7.2-7.4\uff09\u53ca\u8840\u6e05\u6279\u6b21\uff0c\u6392\u9664\u73af\u5883\u56e0\u7d20\u5bfc\u81f4\u7684\u8868\u578b\u6539\u53d8\uff1b\u6362\u6db2\u9891\u7387\u5efa\u8bae 2 \u5929 1 \u6b21\uff0c\u6362\u6db2\u65f6\u4fdd\u7559 1\/3 \u65e7\u57f9\u517b\u57fa\uff08\u542b\u7ec6\u80de\u5206\u6ccc\u7684\u81ea\u5206\u6ccc\u56e0\u5b50\uff09\uff1b\u2462\u201c\u8f6c\u79fb\u9636\u6bb5\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff1a\u642d\u914d COLO205\uff08\u8179\u6c34\u8f6c\u79fb\uff09\u5f00\u5c55\u5b9e\u9a8c\u65f6\uff0c\u53ef\uff1aa. \u68c0\u6d4b\u8f6c\u79fb\u76f8\u5173\u57fa\u56e0\uff08\u5982 MMP2\uff08\u5c40\u90e8\u8f6c\u79fb\u9ad8\u8868\u8fbe\uff09\u3001MMP9\uff08\u8fdc\u5904\u8f6c\u79fb\u9ad8\u8868\u8fbe\uff09\uff09\u7684\u8868\u8fbe\u5dee\u5f02\uff0c\u5206\u6790\u4e0d\u540c\u8f6c\u79fb\u9636\u6bb5\u7684\u5206\u5b50\u7279\u5f81\uff1bb. \u5bf9\u6bd4\u4e24\u682a\u7ec6\u80de\u7684\u6dcb\u5df4\u7ed3\u4fb5\u88ad\u80fd\u529b\uff08Transwell \u5c0f\u5ba4\u94fa Matrigel \u6a21\u62df\u6dcb\u5df4\u7ed3\u5c4f\u969c\uff09\uff0cCOLO 320DM\uff08\u5c40\u90e8\u8f6c\u79fb\uff09\u7684\u7a7f\u819c\u7ec6\u80de\u6570\u5e94\u663e\u8457\u9ad8\u4e8e COLO205\uff08\u8179\u6c34\u8f6c\u79fb\uff09\uff0c\u9a8c\u8bc1\u5c40\u90e8\u8f6c\u79fb\u7ec6\u80de\u7684\u6dcb\u5df4\u7ed3\u5b9a\u690d\u4f18\u52bf\uff1bc. \u68c0\u6d4b\u6fc0\u7d20\u5bf9\u4e24\u682a\u7ec6\u80de\u589e\u6b96\u7684\u5f71\u54cd\uff08\u5982\u6dfb\u52a0 5 &#8211; \u7f9f\u8272\u80fa\uff09\uff0cCOLO 320DM \u53ef\u80fd\u5b58\u5728\u81ea\u5206\u6ccc\u589e\u6b96\u4f18\u52bf\uff1b\u2463\u5f62\u6001\u4e0e\u67d3\u8272\u4f53\u76d1\u6d4b\uff1a\u957f\u671f\u57f9\u517b\u4e2d\uff0c\u6bcf 5 \u4ee3\u89c2\u5bdf\u7ec6\u80de\u5f62\u6001\uff08\u5706\u5f62\u6298\u5149\u7ec6\u80de\u5360\u6bd4\u9700\u226560%\uff09\u3001\u68c0\u6d4b\u67d3\u8272\u4f53\u6570\u76ee\uff0849~52 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