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style=\"list-style-type: none;\">\n<ol start=\"2\">\n<li class=\"temp-li number2\" data-line=\"true\" data-list=\"number2\" data-ol-id=\"7Xy9QVMh\" data-start=\"b\">\u63a5\u79cd\u5bc6\u5ea6\u8981\u6c42\uff1a\u63a5\u79cd\u5bc6\u5ea6\u9700\u4e0d\u4f4e\u4e8e 50%\uff08T25 \u74f6\u63a5\u79cd\u7ec6\u80de\u91cf\u22655\u00d710\u2075cells\uff09\uff0c\u4f4e\u4e8e\u6b64\u5bc6\u5ea6\u4f1a\u5bfc\u81f4\u7ec6\u80de\u751f\u957f\u505c\u6ede\u6216\u51cb\u4ea1\uff1b\u82e5\u9700\u5f00\u5c55\u4f4e\u5bc6\u5ea6\u5b9e\u9a8c\uff08\u5982\u514b\u9686\u5f62\u6210\uff09\uff0c\u9700\u63d0\u524d\u7528\u6761\u4ef6\u57f9\u517b\u57fa\uff08CW2 \u7ec6\u80de\u57f9\u517b\u4e0a\u6e05\u8fc7\u6ee4\u540e\u4f7f\u7528\uff09\u9884\u5904\u7406\u57f9\u517b\u677f\uff0c\u63d0\u5347\u7ec6\u80de\u5b58\u6d3b\u7387\uff1b<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<ol>\n<li style=\"list-style-type: none;\">\n<ol start=\"3\">\n<li class=\"temp-li number2\" data-line=\"true\" data-list=\"number2\" data-ol-id=\"f6aLLvrz\" data-start=\"c\">\u751f\u957f\u76d1\u6d4b\u9891\u7387\uff1a\u56e0\u589e\u6b96\u7f13\u6162\uff0c\u9700\u6bcf 3-4 \u5929\u955c\u68c0 1 \u6b21\u7ec6\u80de\u5bc6\u5ea6\uff0c\u5f85\u5bc6\u5ea6\u8fbe 80%-90% \u65f6\u518d\u4f20\u4ee3\uff08\u5e38\u89c4\u7ec6\u80de 2-3 \u5929\u4f20\u4ee3 1 \u6b21\uff09\uff0c\u907f\u514d\u7ec6\u80de\u8fc7\u5ea6\u5bc6\u96c6\u5bfc\u81f4\u8425\u517b\u8017\u5c3d\u3002<\/li>\n<\/ol>\n<\/li>\n<\/ol>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin\uff1aX,X\uff08\u660e\u786e\u5973\u6027\u6027\u522b\uff0c\u4e0e\u6027\u522b\u63a8\u6d4b\u4e00\u81f4\uff0c\u9a8c\u8bc1\u7ec6\u80de\u8eab\u4efd\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff0c\u4e0e COLO 320DM\uff08Amelogenin\uff1aX\uff09\u5171\u540c\u786e\u8ba4\u5973\u6027\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u8eab\u4efd\uff09; CSF1PO\uff1a11,14\uff1bD12S391\uff1a19,20\uff08\u65b0\u589e\u4f4d\u70b9\uff0c\u533a\u522b\u4e8e COLO 320DM \u65e0\u6b64\u4f4d\u70b9\uff09\uff1bD13S317\uff1a9,11\uff1bD16S539\uff1a9,9\uff08\u7eaf\u5408\u5b50\uff09\uff1bD18S51\uff1a12,13\uff1bD19S433\uff1a12,14.2\uff1bD21S11\uff1a31,31\uff08\u7eaf\u5408\u5b50\uff09\uff1bD2S1338\uff1a18,19\uff1bD3S1358\uff1a16,17\uff1bD5S818\uff1a11,13\uff1bD6S1043\uff1a16,16\uff08\u7eaf\u5408\u5b50\uff09\uff1bD7S820\uff1a10,13\uff1bD8S1179\uff1a15,17\uff1bFGA\uff1a21,22\uff1bPenta E\uff1a11,14\uff08\u65b0\u589e\u4f4d\u70b9\uff0c\u4e0e COLO 320DM \u7684 STR \u56fe\u8c31\u5dee\u5f02\u663e\u8457\uff09\uff1bTH01\uff1a7,8\uff1bTPOX\uff1a8,12\uff1bvWA\uff1a18,21\uff1b\uff08STR \u4f4d\u70b9\u542b\u65b0\u589e D12S391\u3001Penta E \u4f4d\u70b9\u53ca\u591a\u4e2a\u7eaf\u5408\u5b50\uff08\u5982 D16S539\uff1a9,9\uff09\uff0c\u4e0e COLO 320DM\u3001COLO320 HSR \u7684 STR \u56fe\u8c31\u5dee\u5f02\u660e\u663e\uff0c\u53ef\u901a\u8fc7\u56fe\u8c31\u6bd4\u5bf9\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\uff0c\u907f\u514d\u540c\u5668\u5b98\u7ec6\u80de\u6df7\u6dc6\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 72 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226575%\uff08\u56e0\u751f\u957f\u7f13\u6162\uff0c\u590d\u82cf\u540e\u8d34\u58c1\u53ca\u589e\u6b96\u542f\u52a8\u65f6\u95f4\u957f\u4e8e\u5e38\u89c4\u7ec6\u80de\uff09\uff0c\u4e14 CEA \u9633\u6027\u7279\u6027\u3001\u4e0a\u76ae\u6837\u5f62\u6001\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%\uff08\u9ad8\u4e8e\u5e38\u89c4\u53d1\u8d27\u5bc6\u5ea6\uff09\uff0c\u6807\u6ce8 \u201c\u9700\u9ad8\u63a5\u79cd\u5bc6\u5ea6\u4f20\u4ee3\u201d\uff0c\u63d0\u9192\u7528\u6237\u9075\u5faa 1:2 \u4f20\u4ee3\u6bd4\u4f8b\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u7f13\u6162\u589e\u6b96\u8fc7\u7a0b\u53ca CEA \u8868\u8fbe\uff0c\u4fdd\u969c\u4e0e\u5176\u4ed6\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1aRPMI-1640+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u4e0e COLO 320DM \u7684\u57f9\u517b\u57fa\u914d\u65b9\u4e00\u81f4\uff0c\u533a\u522b\u4e8e COLO320 HSR \u7684 DMEM\uff1bRPMI-1640 \u542b\u4e30\u5bcc\u6c28\u57fa\u9178\uff0c\u9002\u914d\u4f4e\u589e\u6b96\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u7684\u4ee3\u8c22\u9700\u6c42\uff0c\u652f\u6301\u5176 CEA \u5408\u6210\u53ca\u7a33\u5b9a\u8d34\u58c1\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u589e\u6b96\u56e0\u5b50\u5f02\u5e38\u52a0\u5feb\u7ec6\u80de\u751f\u957f\uff0c\u6539\u53d8\u5176\u4f4e\u589e\u6b96\u7279\u6027\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de CEA \u8868\u8fbe\u53ca\u751f\u957f\u8282\u594f\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u4e0e COLO 320DM\u3001COLO320 HSR\u3001GES-1 \u7b49\u6240\u6709\u5df2\u68b3\u7406\u7ec6\u80de\u7684\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u4ec5\u9700\u9002\u914d\u4f4e\u589e\u6b96\u7279\u6027\u8c03\u6574\u4f20\u4ee3\u95f4\u9694\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u4f4e\u589e\u6b96\u4e0e\u7eaf\u8d34\u58c1\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u540e\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 80%\uff0c\u6b64\u65f6\u7ec6\u80de\u6d3b\u6027\u6700\u4f73\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 2.5-3.5 \u5206\u949f\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 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