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2\uff09\u3001WPMY-1\uff08\u7b49\u7ea7 2\uff09\uff0c\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u4fbf\u4e8e\u5b9e\u9a8c\u5ba4\u7edf\u4e00\u7ba1\u7406\u4e0d\u540c\u5b89\u5168\u7b49\u7ea7\u7684\u6d88\u5316\u9053\u7ec6\u80de\uff0c\u964d\u4f4e\u5b9e\u9a8c\u64cd\u4f5c\u95e8\u69db\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226585%\uff08\u56e0\u77ed\u500d\u589e\u65f6\u95f4\uff0c\u590d\u82cf\u540e 24 \u5c0f\u65f6\u5373\u53ef\u5feb\u901f\u589e\u6b96\uff09\uff0c\u4e14\u4e0a\u76ae\u6837\u5f62\u6001\u3001\u7624\u7403\u5f62\u6210\u80fd\u529b\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 60%-70%\uff0c\u786e\u4fdd\u5230\u8fbe\u540e\u53ef\u5feb\u901f\u4f20\u4ee3\u6216\u5f00\u5c55\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u5feb\u901f\u589e\u6b96\u53ca\u7624\u7403\u5f62\u6210\uff0c\u4fdd\u969c\u4e0e CW2\u3001COLO 320DM \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CCL-247\uff0cBCRC; 60349\uff0cBCRJ; 0288\uff0cDSMZ; ACC-581\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\u3001DSMZ\uff09\u4e0e\u5730\u533a\u6027\u673a\u6784\uff08BCRC\u3001BCRJ\uff09\u591a\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u6781\u5f3a\uff0c\u751f\u7269\u5b66\u7279\u6027\u7ecf\u5168\u7403\u5b9e\u9a8c\u5ba4\u9a8c\u8bc1\uff0c\u4e0e CW2\uff08\u672a\u660e\u786e\u4fdd\u85cf\uff09\u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u673a\u6784\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1aMcCoy&#8217;s 5a+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528 McCoy&#8217;s 5a \u57f9\u517b\u57fa\uff0c\u4e0e CW2 \u7684 RPMI-1640\u3001COLO 320DM \u7684 RPMI-1640 \u663e\u8457\u4e0d\u540c\uff1bMcCoy&#8217;s 5a \u542b\u72ec\u7279\u6c28\u57fa\u9178\u914d\u6bd4\u53ca\u7ef4\u751f\u7d20\uff0c\u9002\u914d\u5feb\u901f\u589e\u6b96\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u7684\u4ee3\u8c22\u9700\u6c42\uff0c\u652f\u6301\u5176\u77ed\u500d\u589e\u65f6\u95f4\u53ca\u7624\u7403\u5f62\u6210\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u589e\u6b96\u6291\u5236\u56e0\u5b50\u5f71\u54cd\u7ec6\u80de\u5feb\u901f\u751f\u957f\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u7624\u7403\u5f62\u6210\u53ca CEA \u8868\u8fbe\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u5f00\u5c55 3D \u7624\u7403\u57f9\u517b\u65f6\uff0c\u6e7f\u5ea6\u63d0\u5347\u81f3 80%\uff0c\u51cf\u5c11\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u7624\u7403\u5e72\u88c2\uff1b\u4e0e CW2\u3001COLO 320DM\u3001GES-1 \u7b49\u6240\u6709\u5df2\u68b3\u7406\u7ec6\u80de\u7684\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u4ec5\u9700\u5728 3D \u57f9\u517b\u65f6\u8c03\u6574\u8017\u6750\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u77ed\u500d\u589e\u4e0e\u8d34\u58c1\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u63a5\u79cd\u540e 24-36 \u5c0f\u65f6\uff0c\u589e\u6b96\u6d3b\u6027\u6700\u4f73\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 1.5-2.5 \u5206\u949f\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e\u5e38\u89c4\u7ec6\u80de\uff0c\u8865\u507f\u5feb\u901f\u589e\u6b96\u5bfc\u81f4\u7684\u4f20\u4ee3\u9700\u6c42\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 McCoy&#8217;s 5a \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\uff0c\u907f\u514d DMSO \u5bf9\u7ec6\u80de\u589e\u6b96\u53ca\u7624\u7403\u5f62\u6210\u80fd\u529b\u7684\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u500d\u589e\u65f6\u95f4\uff1a~25-48 hours\uff08\u589e\u6b96\u901f\u5ea6\u6781\u5feb\uff0c\u662f\u5df2\u68b3\u7406\u7ed3\u76f4\u80a0\u764c\u7ec6\u80de\u4e2d\u4e0e COLO205 \u5e76\u5217\u6700\u5feb\u7684\uff08\u5feb\u4e8e CW2 \u7684 72-96 \u5c0f\u65f6\uff0c\u4e0e COLO205 \u7684 25-30 \u5c0f\u65f6\u76f8\u8fd1\uff09\uff0c\u5bf9\u6570\u751f\u957f\u671f\u4e3a\u63a5\u79cd\u540e 12-24 \u5c0f\u65f6\uff0c\u9700\u6bcf\u5929\u955c\u68c0 2 \u6b21\u7ec6\u80de\u5bc6\u5ea6\uff0c\u5f85\u5bc6\u5ea6\u8fbe 80% \u65f6\u7acb\u5373\u4f20\u4ee3\uff08\u907f\u514d\u8fc7\u5ea6\u5bc6\u96c6\u5bfc\u81f4\u8425\u517b\u8017\u5c3d\uff09\uff1b\u5b9e\u9a8c\u8bbe\u8ba1\u9700\u9002\u914d\u5feb\u8282\u594f\uff0c\u5982\u836f\u7269\u5904\u7406\u65f6\u95f4\u5efa\u8bae 24-48 \u5c0f\u65f6\uff0c\u57fa\u56e0\u7f16\u8f91\u5b9e\u9a8c\u53ef\u7f29\u77ed\u7b5b\u9009\u5468\u671f\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u81f4\u7624\u6027\uff1aYes, in nude mice.\uff08\u660e\u786e\u7684\u88f8\u9f20\u9ad8\u6210\u7624\u80fd\u529b\uff0c\u6210\u7624\u5468\u671f\u77ed\uff08\u7ed3\u5408 25-48 \u5c0f\u65f6\u500d\u589e\u65f6\u95f4\uff0c\u63a8\u6d4b\u76ae\u4e0b\u6210\u7624 2-3 \u5468\u53ef\u89c1\u660e\u663e\u80bf\u7624\uff09\uff0c\u6210\u7624\u7387\u63a5\u8fd1 100%\uff1b\u5efa\u8bae\u63a5\u79cd\u5bc6\u5ea6\u4e3a 1\u00d710\u2076cells \/ \u53ea\uff08\u4f4e\u4e8e COLO205 \u7684 1\u00d710\u2077cells \/ \u53ea\uff09\uff0c\u6210\u7624\u540e\u901a\u8fc7 HE \u67d3\u8272\u89c2\u5bdf\u4e0a\u76ae\u6837\u80bf\u7624\u5f62\u6001\uff0c\u9a8c\u8bc1\u4f53\u5185\u5916\u7279\u6027\u4e00\u81f4\u6027\uff0c\u9002\u5408\u5f00\u5c55\u4f53\u5185\u836f\u7269 efficacy \u5feb\u901f\u8bc4\u4f30\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7624\u7403\u5f62\u6210\u80fd\u529b\uff1a\u5177\u5907\u7624\u7403\u5f62\u6210\u80fd\u529b\uff08\u9700\u4f7f\u7528\u4e13\u7528 3D \u80bf\u7624\u7403\u57f9\u517b\u57fa\uff0c\u8d27\u53f7 IMC-314\uff09\uff08\u6838\u5fc3\u5e94\u7528\u7279\u6027\uff1a\u2460\u7624\u7403\u5f62\u6210\u6761\u4ef6\uff1a\u4f7f\u7528\u8d85\u4f4e\u5438\u9644 6 \u5b54\u677f\uff0c\u63a5\u79cd\u5bc6\u5ea6 1\u00d710\u2074cells \/ \u5b54\uff0c\u52a0\u5165 IMC-314 \u57f9\u517b\u57fa\uff0c\u57f9\u517b 7-10 \u5929\u53ef\u5f62\u6210\u76f4\u5f84 100-150\u03bcm \u7684\u7403\u5f62\u7ed3\u6784\uff0c\u7624\u7403\u5f62\u6210\u7387\u226580%\uff08\u9ad8\u4e8e CW2 \u7684\u63a8\u6d4b\u503c\uff09\uff1b\u2461\u7624\u7403\u7279\u6027\uff1a\u7624\u7403\u7ec6\u80de\u8868\u8fbe\u80bf\u7624\u5e72\u7ec6\u80de\u6807\u5fd7\u7269\uff08\u5982 CD44\u3001ALDH1\uff09\uff0c\u4e14\u4fdd\u7559 CEA \u8868\u8fbe\u53ca\u5feb\u901f\u589e\u6b96\u7279\u6027\uff0c\u53ef\u7528\u4e8e\u80bf\u7624\u5e72\u7ec6\u80de\u5206\u9009\u3001\u836f\u7269\u6e17\u900f\u5b9e\u9a8c\u53ca\u8010\u836f\u673a\u5236\u7814\u7a76\uff1b\u2462\u64cd\u4f5c\u6ce8\u610f\uff1a3D \u57f9\u517b\u671f\u95f4\u65e0\u9700\u6362\u6db2\uff0c\u907f\u514d\u6643\u52a8\u57f9\u517b\u677f\u5bfc\u81f4\u7624\u7403\u7834\u788e\uff1b\u6536\u96c6\u7624\u7403\u65f6\u7528\u79fb\u6db2\u67aa\u8f7b\u67d4\u5439\u6253\uff0c\u79bb\u5fc3\u8f6c\u901f\u964d\u81f3 800rpm\uff0c\u9632\u6b62\u7ed3\u6784\u7834\u574f\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff1acarcinoembryonic antigen (CEA) 1 ng per 1\u00d710\u2076 cells per 10 days\uff08\u7ed3\u76f4\u80a0\u764c\u6807\u5fd7\u7269\uff0c\u8868\u8fbe\u91cf\u660e\u786e\u4e14\u4f4e\u4e8e CW2\uff08\u672a\u660e\u786e\u5177\u4f53\u503c\uff0c\u4f46\u63a8\u6d4b\u66f4\u9ad8\uff09\uff0c\u53ef\u901a\u8fc7 ELISA \u6cd5\u5b9a\u91cf\u68c0\u6d4b\uff0c\u4e0e CW2 \u5bf9\u6bd4\u9a8c\u8bc1 \u201c\u589e\u6b96\u901f\u5ea6\u4e0e CEA \u8868\u8fbe\u91cf\u201d \u7684\u5173\u8054\uff08\u901a\u5e38\u5feb\u901f\u589e\u6b96\u7ec6\u80de CEA \u8868\u8fbe\u76f8\u5bf9\u8f83\u4f4e\uff09\uff1bCEA \u8868\u8fbe\u7a33\u5b9a\uff0c\u53ef\u4f5c\u4e3a\u7ec6\u80de\u8868\u578b\u7a33\u5b9a\u6027\u7684\u5224\u65ad\u6307\u6807\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 McCoy&#8217;s 5a \u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001\u589e\u6b96\u901f\u5ea6\u9a8c\u8bc1\uff08\u7ed8\u5236 24-48 \u5c0f\u65f6\u751f\u957f\u66f2\u7ebf\uff09\u53ca\u7624\u7403\u5f62\u6210\u9884\u5b9e\u9a8c\uff0c\u786e\u4fdd\u6838\u5fc3\u7279\u6027\u7a33\u5b9a\uff1b\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u53ef\u4e0e CW2\uff081 \u5468\u8d27\u671f\uff09\u540c\u6b65\u4e0b\u5355\uff0c\u4fdd\u969c \u201c\u5feb\u6162\u589e\u6b96\u201d \u5e73\u884c\u5b9e\u9a8c\u7684\u65f6\u95f4\u4e00\u81f4\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u907f\u514d\u5267\u70c8\u98a0\u7c38\uff0c\u51cf\u5c11\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165 37\u2103\u57f9\u517b\u7bb1\uff0c12 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u72b6\u6001\uff0c\u56e0\u589e\u6b96\u5feb\uff0c24 \u5c0f\u65f6\u5185\u9700\u5224\u65ad\u662f\u5426\u4f20\u4ee3\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff1b\u82e5\u540c\u6b65\u8d2d\u4e70 IMC-314 \u57f9\u517b\u57fa\uff0c\u9700\u5355\u72ec\u5e38\u6e29\u5305\u88c5\uff0c\u907f\u514d\u4e0e\u5e72\u51b0\u63a5\u89e6\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u6668\u8fbe\u670d\u52a1\uff0c\u6700\u5927\u9650\u5ea6\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca\u589e\u6b96\u7279\u6027\u6539\u53d8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e CW2\u3001COLO 320DM \u7b49\u7ec6\u80de\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff1b\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u907f\u514d\u751f\u7269\u5371\u5bb3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460\u57f9\u517b\u57fa\u4e0e\u589e\u6b96\u7ef4\u62a4\uff1a\u4e25\u683c\u4f7f\u7528 \u201cMcCoy&#8217;s 5a+10% FBS+PS\u201d \u914d\u65b9\uff0c\u4e0d\u53ef\u4e0e CW2 \u7684 RPMI-1640\u3001COLO 320DM \u7684 RPMI-1640 \u6df7\u7528\uff0c\u907f\u514d\u56e0\u57f9\u517b\u57fa\u5dee\u5f02\u5bfc\u81f4\u7ec6\u80de\u589e\u6b96\u901f\u5ea6\u5f02\u5e38\uff1b\u57f9\u517b\u8fc7\u7a0b\u4e2d\u4fdd\u6301\u57f9\u517b\u7bb1\u6e29\u5ea6\u3001CO\u2082\u6d53\u5ea6\u7a33\u5b9a\uff08CO\u2082\u6d53\u5ea6\u6ce2\u52a8\u9700\uff1c\u00b10.5\u2103\uff09\uff0c\u9632\u6b62\u73af\u5883\u56e0\u7d20\u5f71\u54cd\u5feb\u901f\u589e\u6b96\u8282\u594f\uff1b\u24613D \u7624\u7403\u57f9\u517b\u4f18\u5316\uff1a\u4f7f\u7528 IMC-314 \u57f9\u517b\u57fa\u5f00\u5c55\u7624\u7403\u5b9e\u9a8c\u65f6\uff0c\u63a5\u79cd\u524d\u9700\u786e\u4fdd\u8d85\u4f4e\u5438\u9644\u677f\u5e95\u65e0\u6c14\u6ce1\uff1b\u57f9\u517b\u7b2c 5 \u5929\u53ef\u901a\u8fc7\u5012\u7f6e\u663e\u5fae\u955c\u89c2\u5bdf\u7624\u7403\u5b8c\u6574\u6027\uff0c\u82e5\u51fa\u73b0\u677e\u6563\u7ed3\u6784\uff0c\u9700\u8865\u5145 500\u03bcL \u65b0\u9c9c IMC-314 \u57f9\u517b\u57fa\uff0c\u7ef4\u6301\u8425\u517b\u4f9b\u5e94\uff1b\u2462\u201c\u5feb\u6162\u589e\u6b96\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff1a\u642d\u914d CW2\uff08\u4f4e\u589e\u6b96\uff09\u5f00\u5c55\u5b9e\u9a8c\u65f6\uff0c\u53ef\uff1aa. \u68c0\u6d4b\u7ec6\u80de\u5468\u671f\u76f8\u5173\u57fa\u56e0\uff08\u5982 Cyclin D1\uff0cHCT-116 \u9ad8\u8868\u8fbe\uff09\u3001\u51cb\u4ea1\u76f8\u5173\u57fa\u56e0\uff08\u5982 Bcl-2\uff0cHCT-116 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