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GM-CSF\uff08\u514d\u75ab\u8c03\u63a7\uff09\uff0c\u4f53\u73b0\u8f6c\u79fb\u764c\u7684\u514d\u75ab\u9003\u9038\u8868\u578b\uff1b\u2462\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u7ed3\u76f4\u80a0\u764c\u8f6c\u79fb\u76f8\u5173\u57fa\u56e0\u7b5b\u9009\uff08\u5982\u4e0a\u8c03\u7684\u8f6c\u79fb\u57fa\u56e0\uff09\u3001\u5316\u7597\u8010\u836f\u5dee\u5f02\u7814\u7a76\uff08\u8f6c\u79fb\u764c\u901a\u5e38\u66f4\u8010\u836f\uff09\u3001\u539f\u53d1 &#8211; \u8f6c\u79fb\u514d\u75ab\u5fae\u73af\u5883\u5bf9\u6bd4\uff0c\u540c\u65f6\u53ef\u4f5c\u4e3a ras \u7a81\u53d8 PCR \u68c0\u6d4b\u7684\u9633\u6027\u5bf9\u7167\uff08\u4e0e SW480 \u5171\u4eab\u7a81\u53d8\u4f4d\u70b9\uff09\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin\uff1aX\uff08\u4e0e\u7537\u6027\u6027\u522b\u6807\u6ce8\u77db\u76fe\uff0c\u63a8\u6d4b\u4e3a Y 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\u4e00\u81f4\uff09\uff1bD21S11\uff1a30\uff0c30.2\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e SW480 \u4e00\u81f4\uff0c\u6838\u5fc3\u914d\u5bf9\u6807\u5fd7\uff09\uff1bD2S1338\uff1a24\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e SW480 \u7684 17,24 \u5dee\u5f02\uff0c\u8f6c\u79fb\u540e\u7b49\u4f4d\u57fa\u56e0\u4e22\u5931\uff09\uff1bD3S1358\uff1a15\uff0c16\uff08\u4e0e SW480 \u7684 15 \u7eaf\u5408\u5b50\u5dee\u5f02\uff0c\u8f6c\u79fb\u540e\u67d3\u8272\u4f53\u53d8\u5f02\uff09\uff1bD5S818\uff1a13\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e SW480 \u4e00\u81f4\uff09\uff1bD7S820\uff1a8\uff0c9\uff08\u4e0e SW480 \u7684 8 \u7eaf\u5408\u5b50\u5dee\u5f02\uff0c\u8f6c\u79fb\u540e\u67d3\u8272\u4f53\u53d8\u5f02\uff09\uff1bD8S1179\uff1a13\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e SW480 \u4e00\u81f4\uff09\uff1bFGA\uff1a24\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e SW480 \u4e00\u81f4\uff09\uff1bTH01\uff1a8\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e SW480 \u4e00\u81f4\uff09\uff1bTPOX\uff1a11\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e SW480 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bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u6c61\u67d3\u5f71\u54cd\u539f\u53d1 &#8211; \u8f6c\u79fb\u914d\u5bf9\u5b9e\u9a8c\u7684\u5dee\u5f02\u5206\u6790\uff08\u5982\u57fa\u56e0\u8868\u8fbe\u3001\u4fb5\u88ad\u80fd\u529b\uff09\uff0c\u4fdd\u969c\u4e0e SW480 \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CCL-227\uff0cECACC; 87051203 \uff1b\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u57fa\u7840\u533b\u5b66\u7814\u7a76\u6240\u7ec6\u80de\u8d44\u6e90\u4e2d\u5fc3\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\u4e0e\u56fd\u5185\u673a\u6784\u53cc\u91cd\u4fdd\u85cf\uff0c\u4e0e SW480 \u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff08SW480 \u542b DSMZ \u4fdd\u85cf\uff09\uff0c\u914d\u5bf9\u5173\u7cfb\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u673a\u6784\u5f00\u5c55\u534f\u4f5c\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1aL15+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u4e0e SW480 \u7684\u57f9\u517b\u57fa\u914d\u65b9\u5b8c\u5168\u4e00\u81f4\uff0cL15 \u57f9\u517b\u57fa\u7684\u65e0 CO\u2082\u7f13\u51b2\u4f53\u7cfb\u9002\u914d\u8f6c\u79fb\u7ec6\u80de\u7684\u4ee3\u8c22\u9700\u6c42\uff0c\u652f\u6301\u5176\u5feb\u589e\u6b96\uff1b10% FBS \u9700\u4e0e SW480 \u4f7f\u7528\u540c\u6279\u6b21\uff0c\u907f\u514d\u8840\u6e05\u5dee\u5f02\u5e72\u6270\u914d\u5bf9\u5b9e\u9a8c\uff1bPS \u7ec8\u6d53\u5ea6 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u4e0d\u5f71\u54cd GM-CSF \u8868\u8fbe\u53ca\u8f6c\u79fb\u7279\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a100% \u7a7a\u6c14\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e SW480 \u5b8c\u5168\u4e00\u81f4\uff0c\u6e7f\u5ea6 70%-80%\uff1b\u56e0\u5feb\u589e\u6b96\u5bfc\u81f4\u57f9\u517b\u57fa\u84b8\u53d1\u5feb\uff0c\u9700\u6bcf\u5468\u8865\u5145\u57f9\u517b\u7bb1\u6c34\u76d8\u6c34\u91cf\uff0c\u9632\u6b62\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u53ef\u4e0e SW480 \u5171\u7528\u65e0 CO\u2082\u57f9\u517b\u73af\u5883\uff0c\u4fbf\u4e8e\u540c\u6b65\u7ba1\u7406\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u5feb\u589e\u6b96\u4e0e\u8d34\u58c1\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u671f\u65e9\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 60%\uff09\uff0c0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 1.5-2 \u5206\u949f\uff08\u77ed\u4e8e SW480 \u7684 2-3 \u5206\u949f\uff09\uff0c\u8f7b\u67d4\u5439\u6253\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\uff0c\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\u81f3 3\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e SW480 \u7684 2\u00d710\u2076cells\/mL\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 20 \u5206\u949f\u2192-20\u2103 1.5 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\uff0c\u76f4\u63a5\u63a5\u79cd\u65e0 CO\u2082\u73af\u5883\uff0c\u907f\u514d DMSO \u5f71\u54cd\u589e\u6b96\u76f8\u5173\u86cb\u767d\uff08\u5982 Cyclin D1\uff09\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u67d3\u8272\u4f53\uff1a48~51\uff0cXY\uff08\u660e\u786e XY \u7537\u6027\u6838\u578b\uff0c\u67d3\u8272\u4f53\u6570\u76ee\u6bd4 SW480\uff0851~57\uff09\u5c11 3-6 \u6761\uff0c\u4f53\u73b0\u8f6c\u79fb\u540e\u67d3\u8272\u4f53\u4e22\u5931\uff08\u80bf\u7624\u6f14\u8fdb\u7279\u5f81\uff09\uff0c\u4e0e SW480 \u7684\u4e2d\u4f4e\u5f02\u500d\u4f53\u5f62\u6210 \u201c\u8f6c\u79fb\u540e\u67d3\u8272\u4f53\u7b80\u5316\u201d \u68af\u5ea6\uff0c\u9002\u5408\u7814\u7a76\u67d3\u8272\u4f53\u4e0d\u7a33\u5b9a\u6027\u4e0e\u8f6c\u79fb\u7684\u5173\u8054\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u500d\u589e\u65f6\u95f4\uff1a~20-26 hours\uff08\u589e\u6b96\u901f\u5ea6\u5feb\uff0c\u4e3a \u201cSW\u201d \u7cfb\u5217\u4e2d\u6700\u5feb\uff0c\u63a5\u8fd1 HCT-15\uff1b\u5bf9\u6570\u751f\u957f\u671f\u77ed\uff0c\u9700\u6bcf 12 \u5c0f\u65f6\u955c\u68c0 1 \u6b21\uff0c\u4f20\u4ee3\u95f4\u9694 2-3 \u5929\uff1b\u5b9e\u9a8c\u8bbe\u8ba1\u9700\u9002\u914d\u77ed\u5468\u671f\uff0c\u5982\u836f\u7269\u5904\u7406 12-24 \u5c0f\u65f6\uff0c\u4e0e SW480\uff0830-50 \u5c0f\u65f6\uff09\u7684\u5904\u7406\u65f6\u95f4\u9700\u6309\u589e\u6b96\u6bd4\u8c03\u6574\uff08SW620 \u5904\u7406 24 \u5c0f\u65f6\u2248SW480 \u5904\u7406 48 \u5c0f\u65f6\uff09\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u81f4\u7624\u6027\uff1aYes, in nude mice (Tumors developed within 21 days at 100% frequency (5\/5) in nude mice inoculated subcutaneously with 1\u00d710^7 cells).\uff08\u4e0e SW480 \u63a5\u79cd\u5bc6\u5ea6\u4e00\u81f4\uff0c\u4f46\u6210\u7624\u4f53\u79ef\u6bd4 SW480 \u5927 30%-50%\uff08\u5feb\u589e\u6b96 + \u9ad8\u4fb5\u88ad\uff09\uff0c\u6210\u7624\u75c5\u7406\u663e\u793a\u66f4\u9ad8\u7684\u6838\u5206\u88c2\u8c61\uff1b\u5efa\u8bae\u4e0e SW480 \u540c\u6b65\u5f00\u5c55\u88f8\u9f20\u6210\u7624\uff0c\u5bf9\u6bd4\u80bf\u7624\u751f\u957f\u66f2\u7ebf\u53ca\u8f6c\u79fb\u80fd\u529b\uff08SW620 \u53ef\u80fd\u51fa\u73b0\u80ba\u8f6c\u79fb\uff09\uff0c\u9a8c\u8bc1\u4f53\u5185\u8f6c\u79fb\u7279\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7624\u7403\u5f62\u6210\u80fd\u529b\uff1a\u65e0\u6cd5\u5f62\u6210\u7624\u7403\uff08\u4e0e SW480 \u4e00\u81f4\uff0c\u65e0\u80bf\u7624\u5e72\u7ec6\u80de\u7279\u6027\uff0c\u8868\u660e\u8be5\u60a3\u8005\u7684\u539f\u53d1 &#8211; \u8f6c\u79fb\u8fc7\u7a0b\u4e0d\u4f9d\u8d56\u80bf\u7624\u5e72\u7ec6\u80de\uff0c\u9002\u5408\u5f00\u5c55\u975e\u5e72\u7ec6\u80de\u4f9d\u8d56\u7684\u8f6c\u79fb\u673a\u5236\u7814\u7a76\uff0c\u540c\u65f6\u53ef\u4f5c\u4e3a SW480 \u7624\u7403\u5b9e\u9a8c\u7684\u9634\u6027\u5bf9\u7167\uff0c\u6392\u9664\u975e\u7279\u5f02\u6027\u5e72\u6270\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u6297\u539f\u8868\u8fbe\u60c5\u51b5\uff1aBlood Type A; Rh+\uff08\u4e0e SW480 \u5b8c\u5168\u4e00\u81f4\uff0c\u9a8c\u8bc1\u914d\u5bf9\u540c\u6e90\u6027\uff0c\u53ef\u901a\u8fc7\u8840\u578b\u9274\u5b9a\u8f85\u52a9\u786e\u8ba4\u4e24\u682a\u7ec6\u80de\u7684\u914d\u5bf9\u5173\u7cfb\uff0c\u540c\u65f6\u4e0e SW1116\uff08O \u578b\uff09\u5f62\u6210\u8840\u578b\u5dee\u5f02\uff0c\u7528\u4e8e ABO \u8840\u578b\u4e0e\u8f6c\u79fb\u5173\u8054\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff1aCarcinoembryonic antigen (CEA) 0.15 ng\/10^6 cells\/10 days\uff08\u7ed3\u76f4\u80a0\u764c\u6807\u5fd7\u7269\uff0c\u8868\u8fbe\u91cf\u6781\u4f4e\uff0c\u4e3a SW480 \u7684 1\/4.7\u3001SW1116 \u7684 1\/17693\uff0c\u4e0e SW480 \u5f62\u6210 \u201c\u8f6c\u79fb\u540e CEA \u4e0b\u8c03\u201d \u7279\u5f81\uff0c\u53ef\u7528\u4e8e\u7814\u7a76 CEA \u4e0e\u8f6c\u79fb\u6f5c\u80fd\u7684\u8d1f\u76f8\u5173\u673a\u5236\uff09; transforming growth factor alpha\uff08\u8f6c\u5316\u751f\u957f\u56e0\u5b50 \u03b1\uff0c\u4fc3\u8fdb\u8f6c\u79fb\u7ec6\u80de\u589e\u6b96\uff0c\u8868\u8fbe\u91cf\u9ad8\u4e8e SW480\uff09; matrilysin\uff08\u80bf\u7624\u4fb5\u88ad\u76f8\u5173\u91d1\u5c5e\u86cb\u767d\u9176\uff0c\u4e0e SW480 \u5747\u4e3a\u9634\u6027\uff0c\u8868\u660e\u8be5\u60a3\u8005\u80bf\u7624\u4fb5\u88ad\u4e0d\u4f9d\u8d56\u6b64\u9176\uff09; \u764c\u57fa\u56e0\u8868\u8fbe\u4e0e SW480 \u4e00\u81f4\uff08c-myc\u3001K-ras \u7b49\u9633\u6027\uff09\uff0c\u4f53\u73b0\u914d\u5bf9\u7ec6\u80de\u7684\u5206\u5b50\u540c\u6e90\u6027\uff1b\uff08\u57fa\u56e0\u8868\u8fbe\u5dee\u5f02\uff08\u5982 TGF-\u03b1 \u4e0a\u8c03\u3001CEA \u4e0b\u8c03\uff09\u53ef\u4f5c\u4e3a\u8f6c\u79fb\u76f8\u5173\u5019\u9009\u6807\u5fd7\u7269\uff0c\u901a\u8fc7 RT-PCR \u4e0e SW480 \u5bf9\u6bd4\u9a8c\u8bc1\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u9700\u4e0e SW480 \u540c\u6b65\u57f9\u517b\u9a8c\u8bc1\u914d\u5bf9\u7279\u6027\uff0c\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53d1\u8d27\uff0c\u5efa\u8bae\u4e0e SW480 \u540c\u65f6\u4e0b\u5355\uff0c\u4fdd\u969c\u914d\u5bf9\u5b9e\u9a8c\u7684\u65f6\u95f4\u4e00\u81f4\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8d39\uff0c\u9632\u9707\u5305\u88c5 + 2-8\u2103\u51b0\u888b\uff0c\u8fd0\u8f93\u65f6\u5bc6\u5c01\u57f9\u517b\u74f6\u76d6\uff0c\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165\u65e0 CO\u2082\u57f9\u517b\u7bb1\uff0c12 \u5c0f\u65f6\u5185\u89c2\u5bdf\u7ec6\u80de\u72b6\u6001\uff0c24 \u5c0f\u65f6\u5185\u9996\u6b21\u6362\u6db2\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u52a0\u5e72\u51b0\u8d39\u7528\uff0c\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u786e\u4fdd\u51bb\u5b58\u72b6\u6001\uff09 \u987a\u4e30\u6b21\u6668\u8fbe\uff0c\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\u51cf\u5c11\u7279\u6027\u6539\u53d8<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u4e25\u7981\u4e34\u5e8a\u4f7f\u7528\uff1b\u56e0\u662f\u8f6c\u79fb\u7ec6\u80de\uff0c\u5b9e\u9a8c\u64cd\u4f5c\u9700\u52a0\u5f3a\u751f\u7269\u5b89\u5168\u9632\u62a4\uff0c\u907f\u514d\u6c14\u6eb6\u80f6\u6c61\u67d3<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u64cd\u4f5c\u4ec5\u4f9b\u53c2\u8003\uff0c\u4ee5\u968f\u8d27\u8bf4\u660e\u4e66\u4e3a\u51c6\uff1b\u6838\u5fc3\u5efa\u8bae\uff1a\u2460\u914d\u5bf9\u5b9e\u9a8c\u8bbe\u8ba1\uff1a\u4e0e SW480 \u5bf9\u6bd4\u65f6\uff0c\u9700\u63a7\u5236\u4ee3\u6570\uff08\u5747\u7528 5-8 \u4ee3\uff09\u3001\u57f9\u517b\u6761\u4ef6\u3001\u68c0\u6d4b\u65f6\u95f4\u4e09\u5927\u53d8\u91cf\uff1b\u2461\u8f6c\u79fb\u80fd\u529b\u9a8c\u8bc1\uff1a\u9664 Transwell \u5916\uff0c\u53ef\u5f00\u5c55\u88f8\u9f20\u5c3e\u9759\u8109\u6ce8\u5c04\u5b9e\u9a8c\uff08SW620 \u80ba\u8f6c\u79fb\u7387\u9ad8\u4e8e SW480\uff09\uff1b\u2462\u957f\u671f\u57f9\u517b\uff1a\u6bcf 3 \u4ee3\u68c0\u6d4b STR<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"32\" data-line=\"true\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aSW620 \u4eba\u7ed3\u76f4\u80a0\u817a\u764c\u7ec6\u80de\uff08\u4e0e SW480<\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/sw620%e4%ba%ba%e7%bb%93%e7%9b%b4%e8%82%a0%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        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