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class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u80cc\u666f\u7b80\u4ecb\uff1aCalu-1 \u7ec6\u80de\u6838\u5fc3\u7279\u6027\u53ca\u5e94\u7528\u5982\u4e0b\uff1a\u2460\u8d85\u5fae\u7ed3\u6784\u4e0e\u5206\u5b50\u7279\u8272\uff1a\u8d85\u5fae\u7ed3\u6784\u542b\u4f17\u591a\u5fae\u7ed2\u6bdb\uff08\u5229\u4e8e\u7ec6\u80de\u9ecf\u9644\u8f6c\u79fb\uff09\u3001\u663e\u8457\u7c97\u9762\u5185\u8d28\u7f51\uff08\u589e\u5f3a\u86cb\u767d\u5408\u6210\uff0c\u5982\u8f6c\u79fb\u76f8\u5173\u9176\uff09\u3001\u6eb6\u9176\u4f53\uff08\u53c2\u4e0e\u7ec6\u80de\u4fb5\u88ad\uff09\u3001\u8102\u5305\u542b\u4f53\uff08\u80ba\u8868\u76ae\u7624\u4ee3\u8c22\u7279\u5f81\uff09\uff0c\u65e0\u75c5\u6bd2\u9897\u7c92\uff0c\u4f53\u73b0\u8f6c\u79fb\u7076\u7ec6\u80de\u7684\u7ed3\u6784\u9002\u5e94\u6027\uff1b\u542b H-ras \u764c\u57fa\u56e0\uff08\u4e0e A549 \u7684 KRAS \u7a81\u53d8\u5f62\u6210 ras \u5bb6\u65cf\u4e9a\u578b\u5dee\u5f02\uff09\uff0c\u662f\u7814\u7a76 H-ras \u81f4\u764c\u673a\u5236\u7684\u7406\u60f3\u6a21\u578b\uff1b\u2461\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u80ba\u764c\u808b\u819c\u8f6c\u79fb\u673a\u5236\uff08\u5982\u80f8\u819c\u4fb5\u88ad\u4fe1\u53f7\u901a\u8def\uff09\u3001ras \u4e9a\u578b\u529f\u80fd\u5dee\u5f02\uff08H-ras vs KRAS\uff09\u3001\u80ba\u8868\u76ae\u7624\u75c5\u7406\u7279\u5f81\u7814\u7a76\uff0c\u540c\u65f6\u53ef\u4e0e A549 \u642d\u914d\u5f00\u5c55 \u201c\u539f\u53d1 \/ \u8f6c\u79fb + \u4e0d\u540c ras \u4e9a\u578b\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff0c\u63a2\u7d22 ras \u7a81\u53d8\u4e0e\u80ba\u764c\u8f6c\u79fb\u7684\u5173\u8054\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff08\u5efa\u8bae\u5b9e\u9a8c\u524d\u53c2\u8003\u4fdd\u85cf\u673a\u6784\uff08ATCC HTB-54\uff09\u63d0\u4f9b\u7684 STR \u56fe\u8c31\u8fdb\u884c\u8eab\u4efd\u9a8c\u8bc1\uff0c\u6838\u5fc3\u9274\u522b\u53ef\u901a\u8fc7 H-ras \u8868\u8fbe\u68c0\u6d4b\uff08\u5982 Western Blot\uff09\u53ca\u80ba\u8868\u76ae\u7624\u7279\u5f02\u6027\u6807\u5fd7\u7269\uff08\u5982 CK5\/6\uff09\u68c0\u6d4b\uff0c\u786e\u4fdd\u7ec6\u80de\u8eab\u4efd\u65e0\u8bef\uff0c\u907f\u514d\u4e0e\u5176\u4ed6\u80ba\u764c\u7ec6\u80de\uff08\u5982 A549\uff09\u6df7\u6dc6\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e A549\u3001SW620\u3001GES-1 \u7684\u5b89\u5168\u7b49\u7ea7\u4e00\u81f4\uff0c\u4f4e\u4e8e HGC-27\uff08\u7b49\u7ea7 2\uff09\u3001WPMY-1\uff08\u7b49\u7ea7 2\uff09\uff0c\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u56e0\u662f\u8f6c\u79fb\u7076\u7ec6\u80de\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u907f\u514d\u7ec6\u80de\u6b8b\u7559\u6c61\u67d3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226585%\uff08\u4e2d\u7b49\u589e\u6b96\u7279\u6027\u5bfc\u81f4\u590d\u82cf\u6d3b\u6027\u7a33\u5b9a\uff09\uff0c\u4e14 H-ras \u8868\u8fbe\u3001\u4e0a\u76ae\u6837\u5f62\u6001\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 60%-70%\uff0c\u6807\u6ce8 \u201c\u80ba\u8868\u76ae\u7624 \u2162 \u671f\u808b\u819c\u8f6c\u79fb\u7076\uff0c\u542b H-ras \u764c\u57fa\u56e0\u201d\uff0c\u63d0\u9192\u7528\u6237\u5229\u7528\u8f6c\u79fb\u53ca\u5206\u5b50\u7279\u6027\u5f00\u5c55\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u589e\u6b96\u3001H-ras \u4fe1\u53f7\u901a\u8def\u53ca\u8d85\u5fae\u7ed3\u6784\u5b8c\u6574\u6027\uff0c\u4fdd\u969c\u4e0e A549\u3001SW620 \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; HTB-54\uff0cECACC; 93120818\uff1b\u4e2d\u56fd\u79d1\u5b66\u9662\u5206\u5b50\u7ec6\u80de\u79d1\u5b66\u5353\u8d8a\u521b\u65b0\u4e2d\u5fc3\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\u3001ECACC\uff09\u4e0e\u56fd\u5185\u9876\u5c16\u673a\u6784\u53cc\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u80ba\u8868\u76ae\u7624 \u2162 \u671f\u8f6c\u79fb\u7279\u6027\u3001H-ras \u8868\u8fbe\u53ca\u8d85\u5fae\u7ed3\u6784\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4e0e A549\uff08\u591a\u56fd\u9645\u673a\u6784\u4fdd\u85cf\uff09\u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u673a\u6784\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a89% McCoy&#8217;s 5A+10% FBS+1% PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528 McCoy&#8217;s 5A \u57f9\u517b\u57fa\uff0c\u4e0e A549 \u7684 DMEM\u3001SW620 \u7684 L15 \u663e\u8457\u4e0d\u540c\uff1bMcCoy&#8217;s 5A \u542b\u72ec\u7279\u6c28\u57fa\u9178\u914d\u6bd4\u53ca\u5fae\u91cf\u5143\u7d20\uff0c\u9002\u914d\u80ba\u8868\u76ae\u7624\u8f6c\u79fb\u7ec6\u80de\u7684\u4ee3\u8c22\u9700\u6c42\uff0c\u652f\u6301\u5176 H-ras \u86cb\u767d\u5408\u6210\u53ca\u8d85\u5fae\u7ed3\u6784\u7ef4\u6301\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d ras \u901a\u8def\u6291\u5236\u5242\u5f71\u54cd H-ras \u6d3b\u6027\uff1b\u24621% PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u81f4\u7624\u6027\u53ca\u8f6c\u79fb\u7279\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e A549 \u7684\u5e38\u89c4 CO\u2082\u57f9\u517b\u6761\u4ef6\u4e00\u81f4\uff0c\u533a\u522b\u4e8e SW620 \u7684 100% \u7a7a\u6c14\uff1b\u57f9\u517b\u7bb1\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u4e0e A549\u3001GES-1 \u7b49\u5e38\u89c4\u7ec6\u80de\u7684\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5f00\u5c55 \u201c\u80ba\u539f\u53d1 \/ \u8f6c\u79fb\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u8d34\u58c1\u53ca\u8f6c\u79fb\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 70%\uff0c\u589e\u6b96\u6d3b\u6027\u6700\u4f73\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 2-3 \u5206\u949f\uff08\u957f\u4e8e A549 \u7684 1.5-2 \u5206\u949f\uff0c\u56e0\u8d34\u58c1\u5f3a\u5ea6\u7565\u4f4e\uff09\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7834\u574f\u8d85\u5fae\u7ed3\u6784\uff08\u5982\u5fae\u7ed2\u6bdb\uff09\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2.5\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e A549 \u7684 3\u00d710\u2076cells\/mL\uff0c\u8865\u507f\u8f6c\u79fb\u7ec6\u80de\u51bb\u5b58\u635f\u5931\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 McCoy&#8217;s 5A \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\u4e8e 5% CO\u2082\u73af\u5883\uff0c\u907f\u514d DMSO \u5bf9 H-ras \u86cb\u767d\u529f\u80fd\u53ca\u8d85\u5fae\u7ed3\u6784\u7684\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u500d\u589e\u65f6\u95f4\uff1a~37 hours\uff08\u589e\u6b96\u901f\u5ea6\u4e2d\u7b49\uff0c\u6162\u4e8e A549 \u7684 22 \u5c0f\u65f6\uff08\u5feb\uff09\uff0c\u5feb\u4e8e SW1116 \u7684 96-144 \u5c0f\u65f6\uff08\u6162\uff09\uff0c\u5bf9\u6570\u751f\u957f\u671f\u4e3a\u63a5\u79cd\u540e 24-60 \u5c0f\u65f6\uff0c\u9700\u6bcf 24 \u5c0f\u65f6\u955c\u68c0 1 \u6b21\u7ec6\u80de\u5bc6\u5ea6\uff0c\u4f20\u4ee3\u95f4\u9694 3-4 \u5929\uff1b\u5b9e\u9a8c\u8bbe\u8ba1\u9700\u9002\u914d\u4e2d\u5468\u671f\uff0c\u5982\u836f\u7269\u5904\u7406\u65f6\u95f4\u5efa\u8bae 48-72 \u5c0f\u65f6\uff0cH-ras \u4fe1\u53f7\u901a\u8def\u5b9e\u9a8c\u9700\u5728\u5bf9\u6570\u751f\u957f\u671f\u4e2d\u671f\u5f00\u5c55\uff08\u63a5\u79cd\u540e 36 \u5c0f\u65f6\u5de6\u53f3\uff09\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u81f4\u7624\u6027\uff1aYes, in steroid treated hamsters.\uff08\u660e\u786e\u7684\u7c7b\u56fa\u9187\u5904\u7406\u5730\u9f20\u81f4\u7624\u80fd\u529b\uff0c\u56e0\u662f\u8f6c\u79fb\u7076\u7ec6\u80de\uff0c\u81f4\u7624\u7279\u6027\u53ef\u80fd\u66f4\u663e\u8457\uff08\u5982\u6210\u7624\u901f\u5ea6\u5feb\u4e8e\u539f\u53d1\u7076 A549\uff09\uff1b\u5efa\u8bae\u9009\u62e9 6-8 \u5468\u9f84\u7c7b\u56fa\u9187\u5904\u7406\u5730\u9f20\uff08\u5982\u5730\u585e\u7c73\u677e\u9884\u5904\u7406\uff09\uff0c\u63a5\u79cd\u5bc6\u5ea6\u4e3a 1\u00d710\u2077cells \/ \u53ea\uff08\u4e0e A549 \u4e00\u81f4\uff09\uff0c\u63a5\u79cd\u540e\u6bcf 3 \u5929\u6d4b\u91cf\u80bf\u7624\u4f53\u79ef\uff08\u957f \u00d7 \u5bbd \u00b2\/2\uff09\uff0c28 \u5929\u540e\u89c2\u5bdf\u80bf\u7624\u75c5\u7406\u7279\u5f81\uff08\u5982\u8868\u76ae\u7624\u5178\u578b\u7ed3\u6784\uff09\uff0c\u540c\u65f6\u68c0\u6d4b\u80bf\u7624\u7ec4\u7ec7\u4e2d H-ras \u8868\u8fbe\uff0c\u9a8c\u8bc1\u4f53\u5185\u5916\u7279\u6027\u4e00\u81f4\u6027\uff0c\u9002\u5408\u5f00\u5c55\u80ba\u764c\u8f6c\u79fb\u7076\u4f53\u5185\u836f\u7269 efficacy \u8bc4\u4f30\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff1aBlood Type A; Rh+\uff08\u8840\u578b\u6297\u539f A \u578b\u3001Rh \u9633\u6027\uff0c\u4e0e A549\uff08\u672a\u660e\u786e\u8840\u578b\uff09\u3001SW620\uff08A \u578b Rh+\uff09\u8840\u578b\u4e00\u81f4\uff0c\u53ef\u901a\u8fc7\u8840\u578b\u9274\u5b9a\u5b9e\u9a8c\u8f85\u52a9\u9a8c\u8bc1\u7ec6\u80de\u8eab\u4efd\uff0c\u907f\u514d\u4e0e\u5176\u4ed6\u80ba\u764c\u7ec6\u80de\u4ea4\u53c9\u6c61\u67d3\uff1b\u8840\u578b\u6297\u539f\u4e0e\u80bf\u7624\u514d\u75ab\u76f8\u5173\uff0c\u53ef\u7528\u4e8e\u80ba\u764c\u8f6c\u79fb\u7076\u514d\u75ab\u9003\u9038\u673a\u5236\u7814\u7a76\uff08\u5982 ABO \u8840\u578b\u4e0e\u514d\u75ab\u7ec6\u80de\u6d78\u6da6\u7684\u5173\u8054\uff09\uff0c\u540c\u65f6\u4e3a\u7ec6\u80de\u8eab\u4efd\u591a\u91cd\u9a8c\u8bc1\u63d0\u4f9b\u4f9d\u636e\uff09; HLA A10, A11, B15, Bw35\uff08HLA \u5206\u578b\u660e\u786e\uff0c\u53ef\u7528\u4e8e\u80bf\u7624\u514d\u75ab\u76f8\u5173\u5b9e\u9a8c\uff08\u5982 T \u7ec6\u80de\u8bc6\u522b\u3001\u514d\u75ab\u6cbb\u7597\u9776\u70b9\u7b5b\u9009\uff09\uff0c\u4e0e A549\uff08\u672a\u660e\u786e HLA \u5206\u578b\uff09\u5f62\u6210\u514d\u75ab\u8868\u578b\u5dee\u5f02\u5bf9\u6bd4\uff0c\u9002\u5408\u5f00\u5c55\u80ba\u764c\u4e0d\u540c\u4e9a\u578b\u7684\u514d\u75ab\u539f\u6027\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 McCoy&#8217;s 5A \u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001H-ras \u8868\u8fbe\u68c0\u6d4b\uff08Western Blot\uff09\u53ca\u8d85\u5fae\u7ed3\u6784\u9a8c\u8bc1\uff08\u7535\u955c\u89c2\u5bdf\u5fae\u7ed2\u6bdb\uff09\uff0c\u786e\u4fdd\u6838\u5fc3\u7279\u6027\u7a33\u5b9a\uff1b\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u53ef\u4e0e A549\uff081 \u5468\u8d27\u671f\uff09\u540c\u6b65\u4e0b\u5355\uff0c\u4fdd\u969c \u201c\u80ba\u539f\u53d1 \/ \u8f6c\u79fb\u201d \u5e73\u884c\u5b9e\u9a8c\u7684\u65f6\u95f4\u4e00\u81f4\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u4f7f\u7528\u56fa\u5b9a\u652f\u67b6\u9632\u6b62\u57f9\u517b\u74f6\u6643\u52a8\uff0c\u51cf\u5c11\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165 5% CO\u2082\u57f9\u517b\u7bb1\uff0c24 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u72b6\u6001\uff0c36 \u5c0f\u65f6\u5185\u9996\u6b21\u6362\u6db2\uff0c\u786e\u4fdd\u7ec6\u80de\u5feb\u901f\u9002\u5e94\u73af\u5883\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u6668\u8fbe\u670d\u52a1\uff0c\u6700\u5927\u9650\u5ea6\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca H-ras \u8868\u8fbe\u7279\u6027\u6539\u53d8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e A549\u3001SW620 \u7b49\u7ec6\u80de\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff1b\u56e0\u5177\u5907\u665a\u671f\u8f6c\u79fb\u7279\u6027\u53ca H-ras \u81f4\u764c\u57fa\u56e0\uff0c\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u9700\u59a5\u5584\u5904\u7406\u542b\u8be5\u7ec6\u80de\u7684\u57f9\u517b\u57fa\uff0c\u907f\u514d\u73af\u5883\u66b4\u9732\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff09<\/li>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460H-ras \u5b9e\u9a8c\u8bbe\u8ba1\uff1a\u5f00\u5c55 ras \u4e9a\u578b\u5bf9\u6bd4\u5b9e\u9a8c\u65f6\uff0c\u53ef\u901a\u8fc7 Western Blot\uff08\u4e00\u6297\uff1aanti-H-ras \u6297\u4f53\uff0c\u8d27\u53f7\uff1aab16796\uff09\u68c0\u6d4b Calu-1 \u7684 H-ras \u8868\u8fbe\uff0c\u4e0e A549 \u7684 KRAS \u8868\u8fbe\uff08\u4e00\u6297\uff1aanti-KRAS \u6297\u4f53\uff0c\u8d27\u53f7\uff1aab180772\uff09\u5bf9\u6bd4\uff0c\u5206\u6790\u4e24\u682a\u7ec6\u80de\u7684 ras \u901a\u8def\u6d3b\u6027\uff08\u5982 p-RAF\u3001p-ERK \u8868\u8fbe\uff09\u5dee\u5f02\uff1b\u836f\u7269\u7b5b\u9009\u53ef\u9009\u62e9 H-ras \u7279\u5f02\u6027\u6291\u5236\u5242\uff08\u5982 Tipifarnib\uff0c\u6d53\u5ea6\u68af\u5ea6 0.01-1\u03bcM\uff09\uff0c\u5904\u7406 48 \u5c0f\u65f6\u540e\u7528 CCK-8 \u6cd5\u68c0\u6d4b\u6d3b\u6027\uff0c\u8ba1\u7b97 IC50 \u503c\uff0c\u4e0e A549 \u7684 KRAS \u6291\u5236\u5242\u654f\u611f\u6027\u5bf9\u6bd4\uff1b\u2461\u8d85\u5fae\u7ed3\u6784\u7814\u7a76\uff1a\u89c2\u5bdf\u5fae\u7ed2\u6bdb\u3001\u7c97\u9762\u5185\u8d28\u7f51\u7b49\u7ed3\u6784\u65f6\uff0c\u9700\u6536\u96c6\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff0c\u7528 2.5% \u620a\u4e8c\u919b\u56fa\u5b9a\uff0c\u7ecf\u9507\u9178\u540e\u56fa\u5b9a\u3001\u68af\u5ea6\u8131\u6c34\u3001\u73af\u6c27\u6811\u8102\u5305\u57cb\uff0c\u8d85\u8584\u5207\u7247\u540e\u901a\u8fc7\u900f\u5c04\u7535\u955c\u89c2\u5bdf\uff0c\u53ef\u4e0e A549 \u7684\u8d85\u5fae\u7ed3\u6784\u5bf9\u6bd4\uff0c\u5206\u6790\u8f6c\u79fb\u7076\u7684\u7ed3\u6784\u9002\u5e94\u6027\u6539\u53d8\uff1b\u2462\u80ba\u764c\u8f6c\u79fb\u5b9e\u9a8c\uff1a\u5f00\u5c55\u808b\u819c\u8f6c\u79fb\u6a21\u62df\u5b9e\u9a8c\u65f6\uff0c\u53ef\u5c06 Calu-1 \u7ec6\u80de\u4e0e\u5927\u9f20\u80f8\u819c\u95f4\u76ae\u7ec6\u80de\uff08\u5982 RPMI-2650\uff09\u5171\u57f9\u517b\uff0c\u901a\u8fc7 Transwell \u5171\u57f9\u517b\u4f53\u7cfb\u89c2\u5bdf\u7ec6\u80de\u4fb5\u88ad\u80fd\u529b\uff0c\u540c\u65f6\u68c0\u6d4b\u4fb5\u88ad\u76f8\u5173\u57fa\u56e0\uff08\u5982 MMP2\u3001MMP9\uff09\u7684\u8868\u8fbe\uff0c\u9a8c\u8bc1\u8f6c\u79fb\u7279\u6027\uff1b\u2463\u201c\u539f\u53d1 \/ \u8f6c\u79fb\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff1a\u642d\u914d A549 \u5f00\u5c55\u5b9e\u9a8c\u65f6\uff0c\u53ef\uff1aa. \u68c0\u6d4b\u8f6c\u79fb\u76f8\u5173\u57fa\u56e0\uff08\u5982 CXCR4\uff0cCalu-1 \u9ad8\u8868\u8fbe\uff09\u3001ras \u901a\u8def\u57fa\u56e0\uff08H-ras vs KRAS\uff09\u7684\u5dee\u5f02\uff0c\u5206\u6790\u80ba\u764c\u8fdb\u5c55\u7684\u5206\u5b50\u673a\u5236\uff1bb. \u5bf9\u6bd4\u4e24\u682a\u7ec6\u80de\u5bf9\u5316\u7597\u836f\uff08\u5982\u7d2b\u6749\u9187\uff09\u7684 IC50 \u503c\uff08Calu-1 \u56e0\u8f6c\u79fb\u7279\u6027\u53ef\u80fd IC50 \u66f4\u9ad8\uff09\uff0c\u8bc4\u4f30\u8f6c\u79fb\u5bf9\u836f\u7269\u654f\u611f\u6027\u7684\u5f71\u54cd\uff1bc. \u68c0\u6d4b\u80ba\u8868\u76ae\u7624\u6807\u5fd7\u7269\uff08\u5982 CK5\/6\uff0cCalu-1 \u9ad8\u8868\u8fbe\uff09\u4e0e\u817a\u764c\u6807\u5fd7\u7269\uff08\u5982 TTF-1\uff0cA549 \u9ad8\u8868\u8fbe\uff09\u7684\u5dee\u5f02\uff0c\u9a8c\u8bc1\u75c5\u7406\u4e9a\u578b\u7279\u6027\uff1b\u2464\u957f\u671f\u57f9\u517b\u76d1\u6d4b\uff1a\u6bcf 5 \u4ee3\u68c0\u6d4b H-ras \u8868\u8fbe\uff08Western Blot\uff09\u3001\u500d\u589e\u65f6\u95f4\uff08\u9700\u5728 37 \u5c0f\u65f6\u5de6\u53f3\uff09\u53ca\u7ec6\u80de\u5f62\u6001\uff0c\u82e5\u53d1\u73b0 H-ras \u8868\u8fbe\u4e0b\u964d\u6216\u5f62\u6001\u6539\u53d8\uff0c\u9700\u7acb\u5373\u66f4\u6362\u4f4e\u4ee3\u6570\u79cd\u5b50\u7ec6\u80de\uff1b\u2465\u57f9\u517b\u57fa\u9002\u914d\u6027\uff1a\u82e5\u5b9e\u9a8c\u5ba4\u65e0 McCoy&#8217;s 5A \u57f9\u517b\u57fa\uff0c\u53ef\u5c1d\u8bd5\u7528 RPMI-1640 \u57f9\u517b\u57fa\u66ff\u4ee3\uff0c\u4f46\u9700\u63d0\u524d\u8fdb\u884c\u9002\u5e94\u6027\u57f9\u517b\uff08\u4f20\u4ee3 2-3 \u6b21\uff09\uff0c\u5e76\u68c0\u6d4b H-ras \u8868\u8fbe\u53ca\u589e\u6b96\u901f\u5ea6\uff0c\u786e\u4fdd\u7279\u6027\u65e0\u663e\u8457\u6539\u53d8\u3002<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aCalu-1 \u4eba\u80ba\u764c\u7ec6\u80de\uff08\u4e0e A549\uff08\u80ba\u539f<\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/calu-1%e4%ba%ba%e8%82%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        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