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\u5171\u57f9\u517b\u63a2\u7d22\u6210\u7ea4\u7ef4\u7ec6\u80de\u5bf9\u80bf\u7624\u8f6c\u79fb\u7684\u5f71\u54cd\uff09\u3001\u80ba\u80da\u80ce\u53d1\u80b2\u673a\u5236\uff08\u5982\u7ec6\u80de\u56e0\u5b50\u8c03\u63a7\u80ba\u7ec4\u7ec7\u5206\u5316\uff09\u3001\u6b63\u5e38 &#8211; \u80bf\u7624\u7ec6\u80de\u529f\u80fd\u5bf9\u6bd4\uff08\u5982\u589e\u6b96\u3001\u5206\u6ccc\u5dee\u5f02\uff09\uff0c\u540c\u65f6\u53ef\u4f5c\u4e3a\u836f\u7269\u6bd2\u6027\u68c0\u6d4b\u7684\u6b63\u5e38\u7ec6\u80de\u6a21\u578b\uff08\u8bc4\u4f30\u836f\u7269\u5bf9\u6b63\u5e38\u80ba\u7ec6\u80de\u7684\u5f71\u54cd\uff09\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin:X\uff08\u4e0e\u7537\u6027\u6027\u522b\u6807\u6ce8\u77db\u76fe\uff0c\u63a8\u6d4b\u4e3a STR \u68c0\u6d4b\u65f6 Y \u67d3\u8272\u4f53\u4fe1\u53f7\u5f31\u6216\u80ce\u513f\u671f\u67d3\u8272\u4f53\u672a\u5b8c\u5168\u53d1\u80b2\u6210\u719f\u5bfc\u81f4\uff0c\u5efa\u8bae\u5b9e\u9a8c\u524d\u901a\u8fc7 SRY \u57fa\u56e0 PCR \u9a8c\u8bc1\u6027\u522b\uff0c\u907f\u514d\u5f71\u54cd\u7537\u6027\u80da\u80ce\u76f8\u5173\u5b9e\u9a8c\uff08\u5982\u96c4\u6fc0\u7d20\u5bf9\u80ba\u53d1\u80b2\u7684\u5f71\u54cd\uff09\uff09; CSF1PO:10\uff08\u7eaf\u5408\u5b50\uff09\uff1bD13S317:8\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e Calu-3 \u7684 12 \u7eaf\u5408\u5b50\u5dee\u5f02\u663e\u8457\uff09\uff1bD16S539:9,11\uff1bD18S51:15\uff08\u7eaf\u5408\u5b50\uff09\uff1bD21S11:31.2,32.2\uff1bD3S1358:15,17\uff08\u4e0e Calu-3 \u7684 15,18 \u5dee\u5f02\uff09\uff1bD5S818:11,12\uff1bD7S820:11\uff08\u7eaf\u5408\u5b50\uff09\uff1bD8S1179:12,14\uff1bFGA:21,24\uff1bPentaD:9,14\uff08\u989d\u5916\u68c0\u6d4b\u4f4d\u70b9\uff0c\u533a\u522b\u4e8e\u80ba\u764c\u7ec6\u80de\uff09\uff1bPentaE:21\uff08\u989d\u5916\u68c0\u6d4b\u4f4d\u70b9\uff09\uff1bTH01:6,9\uff1bTPOX:8\uff08\u7eaf\u5408\u5b50\uff09\uff1bvWA:16\uff08\u7eaf\u5408\u5b50\uff09\uff1b\uff08STR \u4f4d\u70b9\u542b\u989d\u5916 PentaD\/PentaE \u4f4d\u70b9\u3001\u591a\u4e2a\u7eaf\u5408\u5b50\uff0c\u4e0e Calu-3\u3001A549 \u7684 STR \u56fe\u8c31\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7\u56fe\u8c31\u4e0e ATCC CCL-153 \u6570\u636e\u6bd4\u5bf9\uff0c\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\u53ca\u6b63\u5e38\u6210\u7ea4\u7ef4\u5c5e\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e Calu-3\u3001A549\u3001BEAS-2B \u7684\u5b89\u5168\u7b49\u7ea7\u4e00\u81f4\uff0c\u4f4e\u4e8e HGC-27\uff08\u7b49\u7ea7 2\uff09\u3001WPMY-1\uff08\u7b49\u7ea7 2\uff09\uff0c\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u56e0\u662f\u6b63\u5e38\u7ec6\u80de\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u5904\u7406\u53ef\u6309\u5e38\u89c4\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u6807\u51c6\uff08121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\uff09\uff0c\u65e0\u9700\u989d\u5916\u7279\u6b8a\u9632\u62a4\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u6210\u7ea4\u7ef4\u7ec6\u80de\u5448\u68ad\u5f62\uff0c\u9700\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u65ad\u88c2\uff0c\u786e\u4fdd\u8ba1\u6570\u51c6\u786e\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226585%\uff08\u80da\u80ce\u7ec6\u80de\u590d\u82cf\u6d3b\u6027\u9ad8\uff09\uff0c\u4e14\u6210\u7ea4\u7ef4\u5f62\u6001\u3001\u6838\u578b\u6b63\u5e38\u7279\u6027\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%-80%\uff08\u9ad8\u4e8e Calu-3 \u7684 50%-60%\uff09\uff0c\u6807\u6ce8 \u201c\u6709\u9650\u7ec6\u80de\u7cfb\uff0c\u5efa\u8bae 10 \u4ee3\u5185\u4f7f\u7528\u201d\uff0c\u63d0\u9192\u7528\u6237\u5173\u6ce8\u4f20\u4ee3\u9650\u5236\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u589e\u6b96\u3001\u5206\u6ccc\u529f\u80fd\u53ca\u6838\u578b\u7a33\u5b9a\u6027\uff0c\u4fdd\u969c\u4e0e Calu-3\u3001A549 \u5e73\u884c\u5b9e\u9a8c\uff08\u5982\u5171\u57f9\u517b\uff09\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CCL-153\uff0cBCRC; 60299\uff0cECACC; 89071902\uff1b\u4e2d\u56fd\u79d1\u5b66\u9662\u5206\u5b50\u7ec6\u80de\u79d1\u5b66\u5353\u8d8a\u521b\u65b0\u4e2d\u5fc3\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\u3001ECACC\uff09\u3001\u5730\u533a\u6027\u673a\u6784\uff08BCRC\uff09\u4e0e\u56fd\u5185\u9876\u5c16\u673a\u6784\u4e09\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u6781\u5f3a\uff0c\u80ce\u513f\u80ba\u6210\u7ea4\u7ef4\u7279\u6027\u3001\u6838\u578b\u6b63\u5e38\u53ca\u6709\u9650\u7ec6\u80de\u7cfb\u5c5e\u6027\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4e0e Calu-3\uff08\u53cc\u673a\u6784\u4fdd\u85cf\uff09\u7684\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u673a\u6784\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1aF12K+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528 F12K \u57f9\u517b\u57fa\uff0c\u4e0e Calu-3 \u7684 MEM\u3001A549 \u7684 DMEM \u663e\u8457\u4e0d\u540c\uff1bF12K \u542b\u4e30\u5bcc\u6c28\u57fa\u9178\u3001\u7ef4\u751f\u7d20\u53ca\u5fae\u91cf\u5143\u7d20\uff0c\u9002\u914d\u80da\u80ce\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7684\u9ad8\u4ee3\u8c22\u9700\u6c42\uff0c\u652f\u6301\u5176\u589e\u6b96\u53ca\u5206\u6ccc\u80ba\u53d1\u80b2\u76f8\u5173\u56e0\u5b50\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u8870\u8001\u56e0\u5b50\u52a0\u901f\u6709\u9650\u7ec6\u80de\u7cfb\u8870\u8001\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u5206\u6ccc\u529f\u80fd\u53ca\u6838\u578b\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e Calu-3\u3001A549 \u7684\u5e38\u89c4 CO\u2082\u57f9\u517b\u6761\u4ef6\u4e00\u81f4\uff0c\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u6210\u7ea4\u7ef4\u7ec6\u80de\u4f38\u5c55\u7684\u80de\u8d28\u65ad\u88c2\uff1b\u4e0e\u5176\u4ed6\u80ba\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5f00\u5c55 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