{"id":210,"date":"2025-08-24T08:57:34","date_gmt":"2025-08-24T00:57:34","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=210"},"modified":"2025-08-24T08:57:35","modified_gmt":"2025-08-24T00:57:35","slug":"nci-h1975%e4%ba%ba%e8%82%ba%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/nci-h1975%e4%ba%ba%e8%82%ba%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"NCI-H1975\u4eba\u80ba\u817a\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" 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data-list=\"bullet1\">\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u6027\u522b\u5e74\u9f84\uff1a\u5973\uff1b\u672a\u660e\u786e\uff08Amelogenin\uff1aX \u660e\u786e\u5973\u6027\u6027\u522b\uff0c\u586b\u8865\u80ba\u817a\u764c\u7ec6\u80de\u6a21\u578b\u4e2d \u201c\u5973\u6027\u975e\u5438\u70df\u60a3\u8005\u201d \u7a7a\u767d\uff0c\u4e0e NCI-H1703\uff08\u7537\u6027\u5438\u70df\uff09\u5f62\u6210 \u201c\u5973\u6027\u975e\u5438\u70df &#8211; \u7537\u6027\u5438\u70df\u201d \u6027\u522b\u5438\u70df\u53cc\u91cd\u5bf9\u7167\uff0c\u4e0e Calu-3\uff08\u7537\u6027\u975e\u5438\u70df\uff09\u5f62\u6210 \u201c\u5973\u6027 &#8211; \u7537\u6027\u201d \u540c\u5438\u70df\u72b6\u6001\u6027\u522b\u5bf9\u7167\uff0c\u4e0e HT-29\uff0844 \u5c81\u5973\u6027\u975e\u5438\u70df\uff09\u5f62\u6210 \u201c\u540c\u6027\u522b\u975e\u5438\u70df\u4e0d\u540c\u5668\u5b98\u80bf\u7624\u201d 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data-list=\"bullet1\">\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4ee3\u6570\u7ec6\u80de\u80fd\u66f4\u597d\u4fdd\u7559 \u201c\u5973\u6027\u975e\u5438\u70df\u7279\u6027\u3001EGFR \u57fa\u56e0\u7279\u5f81\u3001\u817a\u764c\u5f62\u6001\u201d \u6838\u5fc3\u5c5e\u6027\uff0c\u5efa\u8bae\u4f20\u4ee3\u6b21\u6570\u4e0d\u8d85\u8fc7 12 \u4ee3\uff0c\u9632\u6b62\u957f\u671f\u4f20\u4ee3\u5bfc\u81f4\u7279\u6027\u6f02\u79fb\uff0c\u5982 EGFR \u8868\u8fbe\u5f02\u5e38\u3001\u817a\u764c\u6807\u5fd7\u7269\uff08TTF-1\uff09\u4e0b\u964d\u3001\u589e\u6b96\u901f\u5ea6\u504f\u79bb 28-45 \u5c0f\u65f6\u8303\u56f4\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u80cc\u666f\u4ecb\u7ecd\uff1aNCI-H1975 \u7ec6\u80de\u4e8e 1988 \u5e74 7 \u6708\u5efa\u682a\uff0c\u7ec4\u7ec7\u63d0\u4f9b\u8005\u4e3a\u975e\u5438\u70df\u5973\u6027\uff0c\u6838\u5fc3\u7279\u6027\u53ca\u5e94\u7528\u5982\u4e0b\uff1a\u2460\u6027\u522b\u5438\u70df\u80cc\u666f\u7279\u8272\uff1a\u975e\u5438\u70df\u5973\u6027\u6765\u6e90\u4f7f\u5176\u6210\u4e3a\u5973\u6027\u975e\u5438\u70df\u80ba\u817a\u764c\u7814\u7a76\u7684\u4e13\u5c5e\u6a21\u578b\uff0c\u53ef\u7528\u4e8e\u63a2\u7d22\u5973\u6027\u975e\u5438\u70df\u7fa4\u4f53\u80ba\u764c\u53d1\u75c5\u673a\u5236\uff08\u5982\u6fc0\u7d20\u6c34\u5e73\u3001\u73af\u5883\u56e0\u7d20\uff09\u3001\u6027\u522b\u5dee\u5f02\u5bf9\u80ba\u764c\u5206\u5b50\u8868\u578b\uff08\u5982 EGFR \u7a81\u53d8\uff09\u7684\u5f71\u54cd\uff1b\u2461EGFR \u7279\u8272\uff1a\u5177\u5907 EGFR \u57fa\u56e0\u68c0\u6d4b\u4fe1\u606f\uff08\u4e34\u5e8a\u5973\u6027\u975e\u5438\u70df\u80ba\u817a\u764c EGFR \u7a81\u53d8\u7387\u9ad8\uff09\uff0c\u53ef\u7528\u4e8e EGFR \u4fe1\u53f7\u901a\u8def\u7814\u7a76\u3001EGFR \u9776\u5411\u836f\u7269\uff08\u5982\u5409\u975e\u66ff\u5c3c\uff09\u654f\u611f\u6027\u5b9e\u9a8c\uff1b\u2462\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u5973\u6027\u975e\u5438\u70df\u80ba\u817a\u764c\u673a\u5236\u3001EGFR \u9776\u5411\u6cbb\u7597\u3001\u80ba\u764c\u6027\u522b\u5dee\u5f02\u7814\u7a76\uff0c\u540c\u65f6\u53ef\u4e0e NCI-H1703 \u642d\u914d\u5f00\u5c55 \u201c\u9cde\u764c \/ \u817a\u764c + \u5438\u70df \/ \u975e\u5438\u70df + \u7537 \/ \u5973\u201d \u591a\u7ef4\u5ea6\u5bf9\u6bd4\u5b9e\u9a8c\uff0c\u63a2\u7d22\u80ba\u764c\u591a\u56e0\u7d20\u5f71\u54cd\u673a\u5236\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin\uff1aX\uff08\u660e\u786e\u5973\u6027\u6838\u578b\uff0c\u4e0e\u6027\u522b\u6807\u6ce8\u4e00\u81f4\uff0c\u65e0\u6027\u522b\u77db\u76fe\u95ee\u9898\uff0c\u9a8c\u8bc1\u7ec6\u80de\u8eab\u4efd\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff0c\u4e0e NCI-H1703\uff08X,Y\uff09\u3001Calu-3\uff08XX \u77db\u76fe\uff09\u5f62\u6210\u6027\u522b\u6838\u578b\u5bf9\u7167\uff09; CSF1PO\uff1a12\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e NCI-H1703 \u7684 12 \u7eaf\u5408\u5b50\u4e00\u81f4\uff0c\u4f53\u73b0\u80ba\u80bf\u7624\u7ec6\u80de STR \u5171\u6027\uff09\uff1bD13S317\uff1a10\uff0c13\uff08\u4e0e NCI-H1703 \u7684 10 \u7eaf\u5408\u5b50\u5dee\u5f02\uff0c\u4f53\u73b0\u817a\u764c\u4e0e\u9cde\u764c STR \u5dee\u5f02\uff09\uff1bD16S539\uff1a9\uff0c12\uff1bD18S51\uff1a13\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e NCI-H1703 \u7684 17,18 \u5dee\u5f02\u663e\u8457\uff09\uff1bD19S433\uff1a15\uff0c15.2\uff08\u7f55\u89c1\u7b49\u4f4d\u57fa\u56e0\uff0c\u817a\u764c\u7279\u8272\u4f4d\u70b9\uff09\uff1bD21S11\uff1a28\uff08\u7eaf\u5408\u5b50\uff09\uff1bD2S1338\uff1a17\uff08\u7eaf\u5408\u5b50\uff09\uff1bD3S1358\uff1a14\uff0c15\uff08\u4e0e NCI-H1703 \u7684 16,17 \u5dee\u5f02\uff09\uff1bD5S818\uff1a11\uff0c12\uff1bD7S820\uff1a8\uff08\u7eaf\u5408\u5b50\uff09\uff1bD8S1179\uff1a13\uff08\u7eaf\u5408\u5b50\uff09\uff1bFGA\uff1a21\uff0c24\uff1bTH01\uff1a7\uff08\u7eaf\u5408\u5b50\uff09\uff1bTPOX\uff1a8\uff0c11\uff1bvWA\uff1a18\uff08\u7eaf\u5408\u5b50\uff09\uff1b\uff08STR \u4f4d\u70b9\u542b\u7f55\u89c1\u7b49\u4f4d\u57fa\u56e0\uff08D19S433\uff1a15.2\uff09\u3001\u591a\u4e2a\u7eaf\u5408\u5b50\uff0c\u4e0e NCI-H1703 \u7684 OL \u7f3a\u5931\u4f4d\u70b9\u3001Calu-3 \u7684 STR \u56fe\u8c31\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7\u56fe\u8c31\u4e0e ATCC CRL-5908 \u6570\u636e\u6bd4\u5bf9\uff0c\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\u53ca\u80ba\u817a\u764c\u5c5e\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">EGFR \u57fa\u56e0\u68c0\u6d4b\uff1a\u672a\u660e\u786e\u5177\u4f53\u7a81\u53d8\u4f4d\u70b9\uff08\u5efa\u8bae\u53c2\u8003\u968f\u8d27\u68c0\u6d4b\u62a5\u544a\u6216\u901a\u8fc7 Sanger \u6d4b\u5e8f\u9a8c\u8bc1\uff0c\u4e34\u5e8a\u5973\u6027\u975e\u5438\u70df\u80ba\u817a\u764c\u5e38\u89c1 EGFR \u7a81\u53d8\u7c7b\u578b\u4e3a L858R \u70b9\u7a81\u53d8\u6216 19 \u5916\u663e\u5b50\u7f3a\u5931\uff09\uff08\u6838\u5fc3\u5e94\u7528\u4ef7\u503c\uff1a\u2460\u9776\u5411\u6cbb\u7597\u7814\u7a76\uff1a\u82e5\u542b\u654f\u611f\u7a81\u53d8\uff08\u5982 L858R\uff09\uff0c\u53ef\u4f5c\u4e3a EGFR-TKI \u7c7b\u836f\u7269\uff08\u5409\u975e\u66ff\u5c3c\u3001\u5384\u6d1b\u66ff\u5c3c\uff09\u7b5b\u9009\u7684\u9633\u6027\u5bf9\u7167\uff1b\u82e5\u542b\u8010\u836f\u7a81\u53d8\uff08\u5982 T790M\uff09\uff0c\u53ef\u7528\u4e8e\u8010\u836f\u673a\u5236\u7814\u7a76\u53ca\u65b0\u4e00\u4ee3 EGFR \u6291\u5236\u5242\uff08\u5965\u5e0c\u66ff\u5c3c\uff09\u7b5b\u9009\uff1b\u2461\u68c0\u6d4b\u65b9\u6cd5\uff1a\u53ef\u901a\u8fc7 ARMS-PCR\uff08\u6269\u589e\u963b\u6ede\u7a81\u53d8\u7cfb\u7edf\uff09\u6216\u6570\u5b57 PCR \u7cbe\u51c6\u68c0\u6d4b\u7a81\u53d8\u7c7b\u578b\u53ca\u7a81\u53d8\u9891\u7387\uff0c\u786e\u4fdd\u9776\u5411\u5b9e\u9a8c\u7684\u51c6\u786e\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e NCI-H1703\u3001Calu-3\u3001HFL-1 \u7684\u5b89\u5168\u7b49\u7ea7\u4e00\u81f4\uff0c\u4f4e\u4e8e HGC-27\uff08\u7b49\u7ea7 2\uff09\u3001WPMY-1\uff08\u7b49\u7ea7 2\uff09\uff0c\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u56e0\u6d89\u53ca EGFR \u9776\u5411\u836f\u7269\u5b9e\u9a8c\uff0c\u9700\u59a5\u5584\u5904\u7406\u542b\u836f\u7269\u7684\u57f9\u517b\u57fa\uff0c\u907f\u514d\u73af\u5883\u66b4\u9732\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u4e0a\u76ae\u7ec6\u80de\u5448\u591a\u8fb9\u5f62\uff0c\u9700\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u56e2\u5757\u5f62\u6210\uff0c\u786e\u4fdd\u8ba1\u6570\u51c6\u786e\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226585%\uff08\u6c38\u751f\u5316\u764c\u7ec6\u80de\u590d\u82cf\u6d3b\u6027\u7a33\u5b9a\uff09\uff0c\u4e14\u4e0a\u76ae\u6837\u5f62\u6001\u3001EGFR \u8868\u8fbe\u7279\u6027\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 60%-70%\uff08\u4e0e Calu-3 \u76f8\u8fd1\uff09\uff0c\u6807\u6ce8 \u201c\u5973\u6027\u975e\u5438\u70df\u80ba\u817a\u764c\uff0c\u5efa\u8bae\u9a8c\u8bc1 EGFR \u7a81\u53d8\u72b6\u6001\u201d\uff0c\u63d0\u9192\u7528\u6237\u5229\u7528\u6027\u522b\u53ca\u5206\u5b50\u7279\u6027\u5f00\u5c55\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u589e\u6b96\u3001EGFR \u8868\u8fbe\u53ca\u9776\u5411\u836f\u7269\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u4e0e NCI-H1703\u3001Calu-3 \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CRL-5908\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\u5355\u4e00\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u5973\u6027\u975e\u5438\u70df\u80ba\u817a\u764c\u7279\u6027\u3001EGFR \u57fa\u56e0\u7279\u5f81\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4e0e NCI-H1703 \u7684\u53cc\u673a\u6784\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u673a\u6784\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a90% DMEM+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528 DMEM \u57f9\u517b\u57fa\uff0c\u4e0e NCI-H1703 \u7684 RPMI-1640\u3001Calu-3 \u7684 MEM \u663e\u8457\u4e0d\u540c\uff1bDMEM \u542b\u9ad8\u7cd6\uff084.5g\/L\uff09\u53ca\u9002\u914d\u5973\u6027\u80ba\u817a\u764c\u7ec6\u80de\u7684\u8425\u517b\u914d\u6bd4\uff0c\u652f\u6301\u5176\u589e\u6b96\u53ca EGFR \u86cb\u767d\u5408\u6210\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u6fc0\u7d20\u6210\u5206\uff08\u5982\u96cc\u6fc0\u7d20\uff09\u5e72\u6270\u5973\u6027\u80ba\u764c\u76f8\u5173\u901a\u8def\uff08\u5982 EGFR-ERK \u4fe1\u53f7\uff09\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u817a\u764c\u8868\u578b\u53ca EGFR \u6d3b\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e NCI-H1703\u3001Calu-3 \u7684\u5e38\u89c4 CO\u2082\u57f9\u517b\u6761\u4ef6\u4e00\u81f4\uff0c\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u4e0e\u5176\u4ed6\u80ba\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5f00\u5c55 \u201c\u591a\u7ef4\u5ea6\u5bf9\u6bd4\u201d \u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u4e0a\u76ae\u7ec6\u80de\u7279\u6027\u4e0e EGFR \u8868\u8fbe\u9700\u6c42\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 80%\uff0cEGFR \u8868\u8fbe\u7a33\u5b9a\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 2-3 \u5206\u949f\uff08\u4e0e NCI-H1703 \u4e00\u81f4\uff09\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2\u00d710\u2076cells\/mL\uff08\u4e0e NCI-H1703 \u4e00\u81f4\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 DMEM \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\uff0c\u907f\u514d DMSO \u5bf9 EGFR \u86cb\u767d\u6784\u8c61\u53ca\u6d3b\u6027\u7684\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u500d\u589e\u65f6\u95f4\uff1a~28-45 hours\uff08\u589e\u6b96\u901f\u5ea6\u4e2d\u7b49\uff0c\u4e0e NCI-H1703 \u7684 30-40 \u5c0f\u65f6\u76f8\u8fd1\uff0c\u5feb\u4e8e Calu-3 \u7684 35-45 \u5c0f\u65f6\uff0c\u5bf9\u6570\u751f\u957f\u671f\u4e3a\u63a5\u79cd\u540e 24-72 \u5c0f\u65f6\uff0c\u9700\u6bcf 24 \u5c0f\u65f6\u955c\u68c0 1 \u6b21\u7ec6\u80de\u5bc6\u5ea6\uff0c\u4f20\u4ee3\u95f4\u9694 3-5 \u5929\uff1b\u5b9e\u9a8c\u8bbe\u8ba1\u9700\u9002\u914d\u4e2d\u5468\u671f\uff0c\u5982 EGFR \u9776\u5411\u836f\u7269\u5904\u7406\u65f6\u95f4\u5efa\u8bae 48-72 \u5c0f\u65f6\uff0c\u786e\u4fdd\u836f\u7269\u5145\u5206\u4f5c\u7528\u4e8e\u7ec6\u80de\u5468\u671f\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 DMEM \u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001EGFR \u8868\u8fbe\u68c0\u6d4b\uff08Western Blot\uff09\u53ca STR \u56fe\u8c31\u9a8c\u8bc1\uff0c\u786e\u4fdd\u6838\u5fc3\u7279\u6027\u7a33\u5b9a\uff1b\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u53ef\u4e0e NCI-H1703\uff081 \u5468\u8d27\u671f\uff09\u540c\u6b65\u4e0b\u5355\uff0c\u4fdd\u969c \u201c\u9cde\u764c \/ \u817a\u764c + \u7537 \/ \u5973\u201d \u5e73\u884c\u5b9e\u9a8c\u7684\u65f6\u95f4\u4e00\u81f4\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u7528\u56fa\u5b9a\u652f\u67b6\u9632\u6b62\u57f9\u517b\u74f6\u6643\u52a8\uff0c\u51cf\u5c11\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165 5% CO\u2082\u57f9\u517b\u7bb1\uff0c24 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u72b6\u6001\uff0c48 \u5c0f\u65f6\u5185\u9996\u6b21\u6362\u6db2\uff0c\u786e\u4fdd\u7ec6\u80de\u5feb\u901f\u9002\u5e94\u73af\u5883\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u6668\u8fbe\u670d\u52a1\uff0c\u6700\u5927\u9650\u5ea6\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca EGFR \u7279\u6027\u6539\u53d8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e NCI-H1703\u3001Calu-3 \u7b49\u7ec6\u80de\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff1b\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u82e5\u6d89\u53ca EGFR \u9776\u5411\u836f\u7269\uff0c\u9700\u6309\u836f\u7269\u5e9f\u5f03\u7269\u5355\u72ec\u5206\u7c7b\u5904\u7406\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460EGFR \u5b9e\u9a8c\u8bbe\u8ba1\uff1a\u5f00\u5c55\u9776\u5411\u836f\u7269\u7b5b\u9009\u65f6\uff0c\u9996\u5148\u901a\u8fc7 Sanger \u6d4b\u5e8f\u9a8c\u8bc1 EGFR \u7a81\u53d8\u7c7b\u578b\uff08\u63a8\u8350\u5f15\u7269\uff1aEGFR \u5916\u663e\u5b50 19 \u6b63\u5411 5&#8242;-GGAATTGAGCAGCGTGATG-3&#8217;\uff0c\u53cd\u5411 5&#8242;-CAGATGTTTTCTTGTCTGATG-3&#8217;\uff1b\u5916\u663e\u5b50 21 \u6b63\u5411 5&#8242;-GCTCACAGCAAATGAGCTGG-3&#8217;\uff0c\u53cd\u5411 5&#8242;-TGGATGAGCTGCGTGATG-3&#8217;\uff09\uff1b\u82e5\u4e3a\u654f\u611f\u7a81\u53d8\uff0c\u8bbe\u7f6e\u5409\u975e\u66ff\u5c3c\u6d53\u5ea6\u68af\u5ea6\uff080.01-10\u03bcM\uff09\uff0c\u5904\u7406 48 \u5c0f\u65f6\u540e\u7528 CCK-8 \u6cd5\u68c0\u6d4b IC50 \u503c\uff0c\u540c\u65f6\u68c0\u6d4b p-EGFR\u3001p-ERK \u8868\u8fbe\uff08Western Blot\uff09\uff0c\u9a8c\u8bc1\u901a\u8def\u6291\u5236\u6548\u679c\uff1b\u2461\u6027\u522b\u5dee\u5f02\u5b9e\u9a8c\uff1a\u642d\u914d NCI-H1703\uff08\u7537\u6027\u5438\u70df\uff09\u5f00\u5c55\u5b9e\u9a8c\u65f6\uff0c\u53ef\uff1aa. \u68c0\u6d4b\u96cc\u6fc0\u7d20\u53d7\u4f53\uff08ER\u03b1\uff0c\u5973\u6027\u7ec6\u80de\u53ef\u80fd\u9ad8\u8868\u8fbe\uff09\u3001EGFR \u7a81\u53d8\u9891\u7387\u7684\u5dee\u5f02\uff0c\u5206\u6790\u6027\u522b\u5bf9\u80ba\u764c\u5206\u5b50\u7279\u5f81\u7684\u5f71\u54cd\uff1bb. \u5bf9\u6bd4\u4e24\u682a\u7ec6\u80de\u5bf9\u5316\u7597\u836f\uff08\u987a\u94c2\uff09\u53ca\u9776\u5411\u836f\uff08\u5409\u975e\u66ff\u5c3c\uff09\u7684\u654f\u611f\u6027\uff0c\u7537\u6027\u9cde\u764c\u53ef\u80fd\u5bf9\u987a\u94c2\u66f4\u654f\u611f\uff0c\u5973\u6027\u817a\u764c\u53ef\u80fd\u5bf9\u5409\u975e\u66ff\u5c3c\u66f4\u654f\u611f\uff1bc. \u68c0\u6d4b\u5438\u70df\u76f8\u5173\u57fa\u56e0\uff08\u5982 CYP1A1\uff0cNCI-H1703 \u9ad8\u8868\u8fbe\uff09\u7684\u5dee\u5f02\uff0c\u9a8c\u8bc1\u5438\u70df\u80cc\u666f\u5bf9\u57fa\u56e0\u8868\u8fbe\u7684\u5f71\u54cd\uff1b\u2462\u957f\u671f\u57f9\u517b\u76d1\u6d4b\uff1a\u6bcf 5 \u4ee3\u68c0\u6d4b EGFR \u8868\u8fbe\uff08Western Blot\uff09\u3001\u817a\u764c\u6807\u5fd7\u7269\uff08TTF-1\u3001Napsin A\uff09\u53ca\u589e\u6b96\u901f\u5ea6\uff0c\u82e5\u53d1\u73b0 EGFR \u8868\u8fbe\u4e0b\u964d\u6216\u6807\u5fd7\u7269\u5f02\u5e38\uff0c\u9700\u7acb\u5373\u66f4\u6362\u4f4e\u4ee3\u6570\u79cd\u5b50\u7ec6\u80de\uff1b\u2463\u57f9\u517b\u57fa\u9002\u914d\u6027\uff1a\u82e5\u5b9e\u9a8c\u5ba4\u65e0 DMEM \u57f9\u517b\u57fa\uff0c\u53ef\u5c1d\u8bd5\u7528 DMEM\/F12\uff081:1\uff09\u57f9\u517b\u57fa\u66ff\u4ee3\uff0c\u4f46\u9700\u63d0\u524d\u9002\u5e94\u6027\u57f9\u517b\uff08\u4f20\u4ee3 2 \u6b21\uff09\uff0c\u5e76\u68c0\u6d4b EGFR \u8868\u8fbe\u3001\u7ec6\u80de\u5f62\u6001\uff0c\u786e\u4fdd\u817a\u764c\u53ca\u5206\u5b50\u7279\u6027\u65e0\u663e\u8457\u6539\u53d8\u3002<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"24\" data-line=\"true\">&lt;\/dou<\/div>\n<div data-zone-id=\"0\" data-line-index=\"25\" data-line=\"true\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aNCI-H1975 \u4eba\u80ba\u817a\u764c\u7ec6\u80de\uff08STR \u9274<\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/nci-h1975%e4%ba%ba%e8%82%ba%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        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