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data-list=\"bullet1\">\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4ee3\u6570\u7ec6\u80de\u80fd\u66f4\u597d\u4fdd\u7559 \u201c\u6162\u589e\u6b96\u3001\u8f6c\u79fb\u7279\u6027\u3001\u9cde\u764c\u5f62\u6001\u201d \u6838\u5fc3\u5c5e\u6027\uff0c\u5efa\u8bae\u4f20\u4ee3\u6b21\u6570\u4e0d\u8d85\u8fc7 10 \u4ee3\uff08\u5c11\u4e8e NCI-H1975 \u7684 12 \u4ee3\uff09\uff0c\u9632\u6b62\u957f\u671f\u4f20\u4ee3\u5bfc\u81f4\u7279\u6027\u6f02\u79fb\uff0c\u5982\u589e\u6b96\u901f\u5ea6\u52a0\u5feb\uff08\uff1c50 \u5c0f\u65f6\uff09\u3001\u89d2\u8d28\u5c0f\u4f53\u51cf\u5c11\u3001\u8f6c\u79fb\u76f8\u5173\u57fa\u56e0\uff08\u5982 MMP2\uff09\u8868\u8fbe\u4e0b\u964d\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u80cc\u666f\u4ecb\u7ecd\uff1aNCI-H226 \u7ec6\u80de\u4e8e 1980 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\u5c0f\u65f6\u6162\u589e\u6b96\u7279\u6027\u6a21\u62df\u4e34\u5e8a\u6162\u8fdb\u5c55\u578b\u80ba\u9cde\u764c\uff0c\u53ef\u7528\u4e8e\u63a2\u7d22\u6162\u589e\u6b96\u80bf\u7624\u7684\u8f6c\u79fb\u4f18\u52bf\uff08\u5982\u4f4e\u589e\u6b96\u4e0e\u9ad8\u5b58\u6d3b\u7684\u5e73\u8861\uff09\uff1b\u2461\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u7537\u6027\u80ba\u9cde\u764c\u80f8\u8154\u8f6c\u79fb\u673a\u5236\uff08\u5982\u80f8\u819c\u4fb5\u88ad\u4fe1\u53f7\u901a\u8def\uff09\u3001\u6162\u589e\u6b96\u80bf\u7624\u836f\u7269\u7b5b\u9009\uff08\u9700\u5ef6\u957f\u836f\u7269\u5904\u7406\u65f6\u95f4\uff09\u3001\u80ba\u9cde\u764c\u4e0e\u817a\u764c\u7684\u75c5\u7406\u5dee\u5f02\u7814\u7a76\uff0c\u540c\u65f6\u53ef\u4e0e NCI-H1703\uff08\u9cde\u764c\u539f\u53d1\uff09\u642d\u914d\u5f00\u5c55 \u201c\u539f\u53d1 \/ \u8f6c\u79fb\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff0c\u63a2\u7d22\u9cde\u764c\u8f6c\u79fb\u7684\u5206\u5b50\u6f14\u53d8\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin\uff1aX,Y\uff08\u660e\u786e\u7537\u6027\u6838\u578b\uff0c\u4e0e\u6027\u522b\u6807\u6ce8\u4e00\u81f4\uff0c\u65e0\u6027\u522b\u77db\u76fe\u95ee\u9898\uff0c\u9a8c\u8bc1\u7ec6\u80de\u8eab\u4efd\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff0c\u4e0e NCI-H1975\uff08X\uff09\u3001NCI-H1703\uff08X,Y\uff09\u5f62\u6210\u6027\u522b\u6838\u578b\u5bf9\u7167\uff09; CSF1PO:10,11\uff08\u4e0e NCI-H1703 \u7684 12 \u7eaf\u5408\u5b50\u5dee\u5f02\uff0c\u4f53\u73b0\u9cde\u764c\u4e0d\u540c\u7ec6\u80de\u682a STR \u5dee\u5f02\uff09\uff1bD12S391:22,22\uff08\u989d\u5916\u68c0\u6d4b\u4f4d\u70b9\uff0c\u533a\u522b\u4e8e NCI-H1975\u3001NCI-H1703\uff0c\u6838\u5fc3\u9274\u522b\u4f4d\u70b9\uff09\uff1bD13S317:13,14\uff08\u4e0e NCI-H1703 \u7684 10 \u7eaf\u5408\u5b50\u5dee\u5f02\u663e\u8457\uff09\uff1bD16S539:9,12\uff08\u4e0e NCI-H1975 \u4e00\u81f4\uff0c\u4f53\u73b0\u80ba\u80bf\u7624\u7ec6\u80de STR \u5171\u6027\uff09\uff1bD18S51:16,16\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e NCI-H1975 \u7684 13 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bD19S433:15,15\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e NCI-H1975 \u7684 15,15.2 \u5dee\u5f02\uff09\uff1bD21S11:29,32.2\uff1bD2S1338:25,25\uff08\u7eaf\u5408\u5b50\uff0c\u9ad8\u7247\u6bb5\u957f\u5ea6\u7b49\u4f4d\u57fa\u56e0\uff0c\u9cde\u764c\u7279\u8272\uff09\uff1bD2S441:14,14\uff08\u989d\u5916\u68c0\u6d4b\u4f4d\u70b9\uff09\uff1bD3S1358:16,16\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e NCI-H1975 \u7684 14,15 \u5dee\u5f02\uff09\uff1bD5S818:11,12\uff08\u4e0e NCI-H1975 \u4e00\u81f4\uff09\uff1bD6S1043:13,18\uff08\u989d\u5916\u68c0\u6d4b\u4f4d\u70b9\uff09\uff1bD7S820:8,10\uff08\u4e0e NCI-H1975 \u7684 8 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bD8S1179:14,15\uff1bFGA:20,23\uff1bPenta D:9,10\uff08\u989d\u5916\u68c0\u6d4b\u4f4d\u70b9\uff09\uff1bPenta E:12,15\uff08\u989d\u5916\u68c0\u6d4b\u4f4d\u70b9\uff09\uff1bTH01:8,9.3\uff08\u4e0e NCI-H1975 \u4e00\u81f4\uff09\uff1bTPOX:8,1\uff08\u7f55\u89c1\u7b49\u4f4d\u57fa\u56e0\uff0c\u6838\u5fc3\u9274\u522b\u4f4d\u70b9\uff09\uff1b\uff08STR \u4f4d\u70b9\u542b 3 \u4e2a\u989d\u5916\u68c0\u6d4b\u4f4d\u70b9\uff08D12S391\u3001D2S441\u3001D6S1043\uff09\u3001\u7f55\u89c1\u7b49\u4f4d\u57fa\u56e0\uff08TPOX:1\uff09\u53ca\u9ad8\u7247\u6bb5\u957f\u5ea6\u7b49\u4f4d\u57fa\u56e0\uff0c\u4e0e NCI-H1975\u3001NCI-H1703 \u7684 STR \u56fe\u8c31\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7\u56fe\u8c31\u4e0e ATCC CRL-5826 \u6570\u636e\u6bd4\u5bf9\uff0c\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\u53ca\u80ba\u9cde\u764c\u8f6c\u79fb\u5c5e\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e NCI-H1975\u3001NCI-H1703\u3001HFL-1 \u7684\u5b89\u5168\u7b49\u7ea7\u4e00\u81f4\uff0c\u4f4e\u4e8e HGC-27\uff08\u7b49\u7ea7 2\uff09\u3001WPMY-1\uff08\u7b49\u7ea7 2\uff09\uff0c\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u56e0\u662f\u8f6c\u79fb\u7076\u7ec6\u80de\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u907f\u514d\u7ec6\u80de\u6b8b\u7559\u6c61\u67d3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u4e0a\u76ae\u7ec6\u80de\u5448\u591a\u8fb9\u5f62\uff0c\u9700\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u56e2\u5757\u5f62\u6210\uff0c\u786e\u4fdd\u8ba1\u6570\u51c6\u786e\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 72 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226580%\uff08\u6162\u589e\u6b96\u7ec6\u80de\u590d\u82cf\u540e\u8d34\u58c1\u53ca\u589e\u6b96\u542f\u52a8\u65f6\u95f4\u957f\uff09\uff0c\u4e14\u4e0a\u76ae\u6837\u5f62\u6001\u3001\u9cde\u764c\u7279\u6027\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 60%-70%\uff08\u4e0e NCI-H1975 \u76f8\u8fd1\uff09\uff0c\u6807\u6ce8 \u201c\u80ba\u9cde\u764c\u80f8\u8154\u79ef\u6db2\u8f6c\u79fb\uff0c~60 \u5c0f\u65f6\u6162\u589e\u6b96\uff0c\u4f20\u4ee3\u95f4\u9694\u957f\u201d\uff0c\u63d0\u9192\u7528\u6237\u9002\u914d\u6162\u589e\u6b96\u8282\u594f\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u6162\u589e\u6b96\u8fc7\u7a0b\u3001\u9cde\u764c\u6807\u5fd7\u7269\u8868\u8fbe\u53ca\u8f6c\u79fb\u76f8\u5173\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u4e0e NCI-H1975\u3001NCI-H1703 \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CRL-5826\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\u5355\u4e00\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u7537\u6027\u80ba\u9cde\u764c\u8f6c\u79fb\u7279\u6027\u3001\u6162\u589e\u6b96\u5c5e\u6027\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4e0e NCI-H1975 \u7684 ATCC \u5355\u4e00\u4fdd\u85cf\u4f53\u7cfb\u4e00\u81f4\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u673a\u6784\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a1640+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u5373 RPMI-1640 \u57f9\u517b\u57fa\uff0c\u6838\u5fc3\u7279\u8272\uff1a\u2460\u4e0e NCI-H1975 \u7684 DMEM\u3001NCI-H1703 \u7684 RPMI-1640 \u914d\u65b9\u4e00\u81f4\uff08\u57fa\u7840\u57f9\u517b\u57fa\u76f8\u540c\uff0c\u533a\u522b\u4e8e Calu-3 \u7684 MEM\uff09\uff1bRPMI-1640 \u542b\u9002\u914d\u6162\u589e\u6b96\u9cde\u764c\u7ec6\u80de\u7684\u8425\u517b\u914d\u6bd4\uff0c\u652f\u6301\u5176\u89d2\u8d28\u5316\u8fc7\u7a0b\u53ca\u8f6c\u79fb\u76f8\u5173\u86cb\u767d\u5408\u6210\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u751f\u957f\u56e0\u5b50\u5f02\u5e38\u52a0\u5feb\u7ec6\u80de\u589e\u6b96\uff0c\u6539\u53d8\u6162\u589e\u6b96\u7279\u6027\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u9cde\u764c\u8868\u578b\u53ca\u81f4\u7624\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e NCI-H1975\u3001NCI-H1703 \u7684\u5e38\u89c4 CO\u2082\u57f9\u517b\u6761\u4ef6\u4e00\u81f4\uff0c\u6e7f\u5ea6\u7ef4\u6301\u5728 75%-80%\uff08\u9ad8\u4e8e\u5e38\u89c4\u7ec6\u80de\uff09\uff0c\u51cf\u5c11\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u4e0e\u5176\u4ed6\u80ba\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\u5b8c\u5168\u4e00\u81f4\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5f00\u5c55 \u201c\u591a\u7ef4\u5ea6\u5bf9\u6bd4\u201d \u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u4e0a\u76ae\u7ec6\u80de\u7279\u6027\u4e0e\u6162\u589e\u6b96\u9700\u6c42\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u540e\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 80%\uff0c\u589e\u6b96\u6d3b\u6027\u6700\u4f73\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 3-4 \u5206\u949f\uff08\u957f\u4e8e NCI-H1975 \u7684 2-3 \u5206\u949f\uff0c\u56e0\u8d34\u58c1\u5f3a\u5ea6\u7565\u9ad8\uff09\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2.5\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e NCI-H1975 \u7684 2\u00d710\u2076cells\/mL\uff0c\u8865\u507f\u6162\u589e\u6b96\u5bfc\u81f4\u7684\u590d\u82cf\u540e\u751f\u957f\u7f13\u6162\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 40 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 3 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1.5 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