{"id":213,"date":"2025-08-24T09:01:49","date_gmt":"2025-08-24T01:01:49","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=213"},"modified":"2025-08-24T09:01:49","modified_gmt":"2025-08-24T01:01:49","slug":"nci-h3255%e4%ba%ba%e8%82%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/nci-h3255%e4%ba%ba%e8%82%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"NCI-H3255\u4eba\u80ba\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" 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2\uff09\uff1b\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u907f\u514d\u7ec6\u80de\u6b8b\u7559\u6c61\u67d3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u9700\u8f7b\u67d4\u5439\u6253\u4e0a\u76ae\u6837\u7ec6\u80de\uff0c\u907f\u514d\u7ec6\u80de\u56e2\u5757\u5f62\u6210\uff0c\u786e\u4fdd\u8ba1\u6570\u51c6\u786e\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u5efa\u8bae\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5185\u68c0\u6d4b\u5b58\u6d3b\u7387\uff08\u9884\u8ba1\u226580%\uff09\uff0c\u4e14\u8d34\u58c1\u7279\u6027\u3001\u4e0a\u76ae\u5f62\u6001\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 60%-70%\uff0c\u6807\u6ce8 \u201c\u9700 15% FBS \u57f9\u517b\u57fa\uff0c\u5efa\u8bae\u590d\u82cf\u540e\u4f18\u5148\u9a8c\u8bc1\u7ec6\u80de\u8eab\u4efd\u201d\uff0c\u63d0\u9192\u7528\u6237\u9002\u914d\u9ad8\u8840\u6e05\u57f9\u517b\u6761\u4ef6\u53ca\u8eab\u4efd\u9a8c\u8bc1\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u8d34\u58c1\u3001\u589e\u6b96\u53ca\u540e\u7eed\u5b9e\u9a8c\uff08\u5982\u836f\u7269\u7b5b\u9009\uff09\uff0c\u4fdd\u969c\u4e0e\u5176\u4ed6\u80ba\u80bf\u7624\u7ec6\u80de\u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff08\u5efa\u8bae\u5b9e\u9a8c\u524d\u54a8\u8be2\u4f9b\u5e94\u5546\u83b7\u53d6\u4fdd\u85cf\u673a\u6784\u4fe1\u606f\uff08\u5982 ATCC\u3001ECACC \u6216\u56fd\u5185\u6743\u5a01\u673a\u6784\uff09\uff0c\u901a\u8fc7\u4fdd\u85cf\u673a\u6784\u6570\u636e\u5e93\u67e5\u8be2\u7ec6\u80de\u80cc\u666f\uff08\u5982\u75c5\u7406\u4e9a\u578b\u3001\u6027\u522b\u5e74\u9f84\uff09\uff0c\u8865\u5145\u5b8c\u5584\u7ec6\u80de\u57fa\u7840\u4fe1\u606f\uff0c\u63d0\u5347\u5b9e\u9a8c\u53ef\u8ffd\u6eaf\u6027\u4e0e\u7ed3\u679c\u91cd\u590d\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a1640+15% FBS+1% PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528 RPMI-1640 \u57f9\u517b\u57fa\uff0c\u4e0e NCI-H292 \u7684 1640 \u57f9\u517b\u57fa\u4e00\u81f4\uff0c\u533a\u522b\u4e8e NCI-H1975 \u7684 DMEM\uff1b15% FBS \u4e3a\u9ad8\u8840\u6e05\u6d53\u5ea6\uff08\u9ad8\u4e8e\u540c\u7c7b\u80ba\u764c\u7ec6\u80de\u7684 10% FBS\uff09\uff0c\u9002\u914d\u7ec6\u80de\u9ad8\u8425\u517b\u9700\u6c42\uff0c\u53ef\u80fd\u4e0e\u7ec6\u80de\u4ee3\u8c22\u6d3b\u8dc3\u6216\u589e\u6b96\u4f9d\u8d56\u76f8\u5173\uff0c\u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u8d28\u91cf\u5f71\u54cd\u7ec6\u80de\u7279\u6027\uff1b\u24611% PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u8d34\u58c1\u4e0e\u589e\u6b96\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e NCI-H292\u3001NCI-H1975 \u7684\u5e38\u89c4 CO\u2082\u57f9\u517b\u6761\u4ef6\u4e00\u81f4\uff0c\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\u53ca\u8840\u6e05\u6d53\u5ea6\u5f02\u5e38\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u4e0e\u5176\u4ed6\u80ba\u80bf\u7624\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\u517c\u5bb9\u6027\u9ad8\uff0c\u4ec5\u9700\u6ce8\u610f 15% FBS \u7684\u5355\u72ec\u914d\u7f6e\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u8d34\u58c1\u53ca\u9ad8\u8840\u6e05\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 80%\uff0c\u6d3b\u6027\u6700\u4f73\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 2-3 \u5206\u949f\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2.5\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e\u5e38\u89c4 10% FBS \u7ec6\u80de\uff0c\u8865\u507f\u9ad8\u8840\u6e05\u7ec6\u80de\u51bb\u5b58\u635f\u5931\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 15% FBS 1640 \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\uff0c\u907f\u514d DMSO \u53ca\u8840\u6e05\u6d53\u5ea6\u9aa4\u964d\u5f71\u54cd\u7ec6\u80de\u6d3b\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 15% FBS 1640 \u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001\u652f\u539f\u4f53\u68c0\u6d4b\u53ca\u7ec6\u80de\u5f62\u6001\u9a8c\u8bc1\uff0c\u786e\u4fdd\u6838\u5fc3\u57f9\u517b\u7279\u6027\u7a33\u5b9a\uff1b\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u56e0\u9700 15% \u9ad8\u8840\u6e05\uff0c\u5efa\u8bae\u7528\u6237\u63d0\u524d\u51c6\u5907\u5bf9\u5e94\u57f9\u517b\u57fa\uff0c\u907f\u514d\u5230\u8d27\u540e\u5ef6\u8bef\u57f9\u517b\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u7528\u56fa\u5b9a\u652f\u67b6\u9632\u6b62\u57f9\u517b\u74f6\u6643\u52a8\uff0c\u51cf\u5c11\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165 5% CO\u2082\u57f9\u517b\u7bb1\uff0c24 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u72b6\u6001\uff0c48 \u5c0f\u65f6\u5185\u9996\u6b21\u6362\u6db2\uff08\u4f7f\u7528 15% FBS 1640 \u57f9\u517b\u57fa\uff09\uff0c\u786e\u4fdd\u7ec6\u80de\u9002\u5e94\u73af\u5883\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u6668\u8fbe\u670d\u52a1\uff0c\u6700\u5927\u9650\u5ea6\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca\u9ad8\u8840\u6e05\u9002\u5e94\u7279\u6027\u6539\u53d8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e NCI-H292\u3001NCI-H1975 \u7b49\u7ec6\u80de\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff1b\u5b9e\u9a8c\u8fc7\u7a0b\u4e2d\u9700\u59a5\u5584\u5904\u7406\u542b\u9ad8\u8840\u6e05\u7684\u57f9\u517b\u57fa\uff0c\u907f\u514d\u73af\u5883\u6c61\u67d3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460\u9ad8\u8840\u6e05\u57f9\u517b\u7ba1\u7406\uff1a\u4e25\u683c\u4f7f\u7528 15% FBS \u914d\u7f6e\u57f9\u517b\u57fa\uff0c\u907f\u514d\u64c5\u81ea\u964d\u4f4e\u8840\u6e05\u6d53\u5ea6\uff08\u53ef\u80fd\u5bfc\u81f4\u7ec6\u80de\u589e\u6b96\u51cf\u6162\u3001\u8d34\u58c1\u8131\u843d\uff09\uff1b\u66f4\u6362\u8840\u6e05\u6279\u6b21\u65f6\uff0c\u9700\u5148\u8fdb\u884c\u5c0f\u4f53\u79ef\u9002\u5e94\u6027\u57f9\u517b\uff08\u5982 T25 \u74f6\u63a5\u79cd 1\u00d710\u2075cells\uff09\uff0c\u89c2\u5bdf 2-3 \u4ee3\uff0c\u786e\u8ba4\u7ec6\u80de\u5f62\u6001\u3001\u589e\u6b96\u65e0\u5f02\u5e38\u540e\u518d\u6279\u91cf\u4f7f\u7528\uff1b\u2461\u7ec6\u80de\u8eab\u4efd\u4e0e\u4e9a\u578b\u9a8c\u8bc1\uff1a\u5efa\u8bae\u9996\u6b21\u57f9\u517b\u65f6\uff0c\u901a\u8fc7 STR \u68c0\u6d4b\uff08\u8986\u76d6\u81f3\u5c11 16 \u4e2a\u6838\u5fc3\u4f4d\u70b9\uff09\u9a8c\u8bc1\u7ec6\u80de\u8eab\u4efd\uff0c\u540c\u65f6\u91c7\u7528\u514d\u75ab\u8367\u5149\u6216 Western Blot \u68c0\u6d4b\u80ba\u80bf\u7624\u4e9a\u578b\u6807\u5fd7\u7269\uff08\u817a\u764c\uff1aTTF-1\u3001Napsin A\uff1b\u9cde\u764c\uff1aCK5\/6\u3001p63\uff09\uff0c\u660e\u786e\u75c5\u7406\u4e9a\u578b\uff0c\u4e3a\u540e\u7eed\u5b9e\u9a8c\u8bbe\u8ba1\u63d0\u4f9b\u4f9d\u636e\uff1b\u2462\u589e\u6b96\u7279\u6027\u63a2\u7d22\uff1a\u901a\u8fc7 CCK-8 \u6cd5\u7ed8\u5236\u7ec6\u80de\u751f\u957f\u66f2\u7ebf\uff0c\u68c0\u6d4b 0-120 \u5c0f\u65f6\u7ec6\u80de\u5bc6\u5ea6\u53d8\u5316\uff0c\u8ba1\u7b97\u500d\u589e\u65f6\u95f4\uff08\u9884\u8ba1 35-50 \u5c0f\u65f6\uff09\uff0c\u786e\u5b9a\u5bf9\u6570\u751f\u957f\u671f\uff08\u5efa\u8bae\u63a5\u79cd\u540e 24-72 \u5c0f\u65f6\uff09\uff0c\u4e3a\u836f\u7269\u5904\u7406\u3001\u8f6c\u67d3\u7b49\u5b9e\u9a8c\u63d0\u4f9b\u6700\u4f73\u65f6\u95f4\u7a97\u53e3\uff1b\u2463\u4e0e\u540c\u7c7b\u7ec6\u80de\u5bf9\u6bd4\u5b9e\u9a8c\uff1a\u642d\u914d NCI-H292\uff0810% FBS\uff09\u5f00\u5c55\u8840\u6e05\u6d53\u5ea6\u5f71\u54cd\u5b9e\u9a8c\u65f6\uff0c\u53ef\u8bbe\u7f6e 10%\u300115%\u300120% FBS 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