{"id":215,"date":"2025-08-24T09:20:04","date_gmt":"2025-08-24T01:20:04","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=215"},"modified":"2025-08-24T09:20:05","modified_gmt":"2025-08-24T01:20:05","slug":"nci-h520%e4%ba%ba%e8%82%ba%e9%b3%9e%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/nci-h520%e4%ba%ba%e8%82%ba%e9%b3%9e%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"NCI-H520\u4eba\u80ba\u9cde\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" 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Gazdar \u56e2\u961f\u4ece\u80ba\u9cde\u764c\u60a3\u8005\u80ba\u6807\u672c\u4e2d\u5efa\u7acb\uff0c\u6838\u5fc3\u7279\u6027\u53ca\u5e94\u7528\u5982\u4e0b\uff1a\u2460\u5206\u5b50\u7279\u8272\uff1ap53 mRNA \u4f4e\u8868\u8fbe\u4f46\u65e0\u5927\u7684 DNA \u7ed3\u6784\u5f02\u5e38\uff08\u533a\u522b\u4e8e\u5176\u4ed6\u80ba\u764c\u7ec6\u80de\u7684 p53 \u7a81\u53d8\uff09\uff0c\u89d2\u86cb\u767d\uff08\u4e0a\u76ae\u6807\u5fd7\u7269\uff09\u3001\u6ce2\u5f62\u86cb\u767d\uff08\u95f4\u8d28\u6807\u5fd7\u7269\uff09\u53cc\u9633\u6027\uff08\u63d0\u793a EMT \u503e\u5411\uff09\uff0c\u662f\u7814\u7a76 p53 \u8868\u8fbe\u8c03\u63a7\u53ca EMT \u65e9\u671f\u9636\u6bb5\u7684\u7406\u60f3\u6a21\u578b\uff1b\u2461\u529f\u80fd\u7279\u8272\uff1a\u8f6f\u743c\u8102\u514b\u9686\u80fd\u529b\u53ca\u88f8\u9f20\u81f4\u7624\u6027\uff0821 \u5929 100% \u6210\u7624\uff09\uff0c\u9002\u5408\u80bf\u7624\u5e72\u7ec6\u80de\u53ca\u4f53\u5185\u836f\u6548\u5b9e\u9a8c\uff1b\u2462\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u80ba\u9cde\u764c\u539f\u53d1\u673a\u5236\u3001p53 \u4f4e\u8868\u8fbe\u529f\u80fd\u3001EMT \u503e\u5411\u7814\u7a76\uff0c\u540c\u65f6\u53ef\u4e0e NCI-H226\uff08\u9cde\u764c\u8f6c\u79fb\uff09\u642d\u914d\u5f00\u5c55 \u201c\u539f\u53d1 \/ \u8f6c\u79fb\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff0c\u63a2\u7d22 EMT \u4e0e\u8f6c\u79fb\u7684\u5173\u8054\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin: X\uff08\u6027\u522b\u6838\u578b\u5f85\u786e\u8ba4\uff0c\u9700\u7ed3\u5408 SRY \u57fa\u56e0 PCR \u9a8c\u8bc1\uff0c\u907f\u514d\u56e0 Y \u67d3\u8272\u4f53\u7f3a\u5931\u5bfc\u81f4\u6027\u522b\u8bef\u5224\uff09; CSF1PO: 10\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e NCI-H441 \u7684 11,12 \u5dee\u5f02\uff09\uff1bD13S317: 10,11\uff08\u4e0e NCI-H226 \u7684 13,14 \u5dee\u5f02\u663e\u8457\uff09\uff1bD16S539: 8,13\uff08\u4e0e NCI-H441 \u7684 9,13 \u5dee\u5f02\uff09\uff1bD5S818: 12,13\uff08\u4e0e NCI-H441 \u7684 11,12 \u5dee\u5f02\uff09\uff1bD7S820: 8,12\uff08\u4e0e NCI-H226 \u7684 8,10 \u5dee\u5f02\uff09\uff1bTH01: 10\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e NCI-H441 \u7684 9.3,9.3 \u5dee\u5f02\uff09\uff1bTPOX: 8\uff08\u7eaf\u5408\u5b50\uff09\uff1bvWA: 18,19\uff08\u9ad8\u7247\u6bb5\u7b49\u4f4d\u57fa\u56e0\uff0c\u9cde\u764c\u7279\u8272\uff09\uff1b\uff08STR \u4f4d\u70b9\u542b\u9ad8\u7247\u6bb5\u7b49\u4f4d\u57fa\u56e0\u53ca\u591a\u4e2a\u7eaf\u5408\u5b50\uff0c\u4e0e NCI-H441\u3001NCI-H226 \u7684 STR \u56fe\u8c31\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7\u56fe\u8c31\u4e0e ATCC HTB-182 \u6570\u636e\u6bd4\u5bf9\uff0c\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\u53ca\u80ba\u9cde\u764c\u5c5e\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e NCI-H441\u3001NCI-H226\u3001HFL-1 \u4e00\u81f4\uff0c\u4f4e\u4e8e HGC-27\uff08\u7b49\u7ea7 2\uff09\uff1b\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u89c4\u8303\uff0c\u56e0\u5b58\u5728 EMT \u503e\u5411\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\uff0c\u907f\u514d\u7ec6\u80de\u6b8b\u7559\u6c61\u67d3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u9700\u8f7b\u67d4\u5439\u6563\u7ec6\u80de\u56e2\uff0c\u786e\u4fdd\u8ba1\u6570\u51c6\u786e\uff1b\u51bb\u5b58\u7ba1\u542b 10% DMSO \u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226580%\uff08\u5bbd\u8303\u56f4\u500d\u589e\u5bfc\u81f4\u90e8\u5206\u7ec6\u80de\u590d\u82cf\u6d3b\u6027\u5dee\u5f02\uff09\uff0c\u4e14\u53cc\u9633\u6027\u6807\u5fd7\u7269\u3001\u4e0a\u76ae\u5f62\u6001\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 60%\uff0c\u6807\u6ce8 \u201c\u80ba\u9cde\u764c\u539f\u53d1\u7076\uff0cp53 \u4f4e\u8868\u8fbe\uff0c\u500d\u589e\u65f6\u95f4\u6ce2\u52a8\u5927\u201d\uff0c\u63d0\u9192\u7528\u6237\u5173\u6ce8\u589e\u6b96\u5f02\u8d28\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u6c61\u67d3\u5f71\u54cd p53 \u8868\u8fbe\u53ca\u8f6f\u743c\u8102\u514b\u9686\u5b9e\u9a8c\uff0c\u4fdd\u969c\u4e0e\u5176\u4ed6\u80ba\u7ec6\u80de\u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; HTB-182\uff1b\u4e2d\u56fd\u79d1\u5b66\u9662\u6606\u660e\u7ec6\u80de\u5e93\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\u4e0e\u56fd\u5185\u673a\u6784\u53cc\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u80ba\u9cde\u764c\u539f\u53d1\u7279\u6027\u3001p53 \u8868\u8fbe\u7279\u5f81\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4e0e NCI-H441 \u7684 ATCC \u53cc\u7f16\u53f7\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u534f\u4f5c\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1aRPMI-1640+10% FBS+PS\uff08\u4e0e NCI-H441 \u7684\u57f9\u517b\u57fa\u914d\u65b9\u4e00\u81f4\uff0c\u533a\u522b\u4e8e NCI-H226 \u7684\u540c\u914d\u65b9\u4f46\u4e0d\u540c\u5e94\u7528\u573a\u666f\uff1bRPMI-1640 \u542b\u9002\u914d\u9cde\u764c\u539f\u53d1\u7ec6\u80de\u7684\u8425\u517b\u914d\u6bd4\uff0c\u652f\u6301\u5176\u5bbd\u8303\u56f4\u500d\u589e\u53ca\u53cc\u9633\u6027\u6807\u5fd7\u7269\u8868\u8fbe\uff1b10% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u6279\u6b21\uff0c\u907f\u514d\u5f71\u54cd p53 mRNA \u8868\u8fbe\uff1bPS \u7ec8\u6d53\u5ea6 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u4e0d\u5f71\u54cd\u81f4\u7624\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e NCI-H441\u3001NCI-H226 \u7684\u5e38\u89c4 CO\u2082\u6761\u4ef6\u4e00\u81f4\uff0c\u6e7f\u5ea6 70%-80%\uff1b\u56e0\u589e\u6b96\u5f02\u8d28\u6027\uff0c\u9700\u5b9a\u671f\u89c2\u5bdf\u7ec6\u80de\u5bc6\u5ea6\uff0c\u907f\u514d\u90e8\u5206\u5feb\u589e\u6b96\u7ec6\u80de\u8fc7\u5ea6\u751f\u957f\uff1b\u57f9\u517b\u73af\u5883\u4e0e\u5176\u4ed6\u80ba\u7ec6\u80de\u517c\u5bb9\uff0c\u65e0\u9700\u7279\u6b8a\u8c03\u6574\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u589e\u6b96\u5f02\u8d28\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u4e2d\u671f\u7ec6\u80de\uff08\u517c\u987e\u5feb\u6162\u589e\u6b96\u4e9a\u7fa4\uff09\uff0c0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 3 \u5206\u949f\uff0c\u8f7b\u67d4\u5439\u6253\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\uff0c\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\u81f3 2.5\u00d710\u2076cells\/mL\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\uff0c\u7528\u9884\u70ed\u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u907f\u514d DMSO \u5f71\u54cd p53 \u86cb\u767d\u529f\u80fd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u67d3\u8272\u4f53\uff1a55-60\uff08\u4e2d\u9ad8\u5f02\u500d\u4f53\uff0c\u67d3\u8272\u4f53\u6570\u76ee\u9ad8\u4e8e NCI-H441\uff08\u672a\u660e\u786e\uff09\u3001\u4f4e\u4e8e NCI-H226\uff08\u672a\u660e\u786e\uff09\uff0c\u6838\u578b\u7a33\u5b9a\uff1b\u5f02\u500d\u4f53\u7279\u5f81\u53ef\u80fd\u4e0e\u5bbd\u8303\u56f4\u500d\u589e\u76f8\u5173\uff0c\u5b9e\u9a8c\u8bbe\u8ba1\u9700\u6ce8\u610f\u67d3\u8272\u4f53\u6570\u76ee\u5bf9\u57fa\u56e0\u8868\u8fbe\u68c0\u6d4b\u7684\u6f5c\u5728\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u500d\u589e\u65f6\u95f4\uff1a~32-60 hours\uff08\u589e\u6b96\u5f02\u8d28\u6027\u6838\u5fc3\u7279\u5f81\uff0c\u9700\u901a\u8fc7 CCK-8 \u6cd5\u7ed8\u5236\u751f\u957f\u66f2\u7ebf\u786e\u5b9a\u5177\u4f53\u6279\u6b21\u500d\u589e\u65f6\u95f4\uff08\u5efa\u8bae\u68c0\u6d4b 3-5 \u5929\uff09\uff1b\u5b9e\u9a8c\u8bbe\u8ba1\u9700\u9002\u914d\u5bbd\u8303\u56f4\uff0c\u5982\u836f\u7269\u5904\u7406\u65f6\u95f4\u5efa\u8bae 72 \u5c0f\u65f6\uff0c\u786e\u4fdd\u8986\u76d6\u5b8c\u6574\u589e\u6b96\u5468\u671f\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u81f4\u7624\u6027\uff1aYes, in nude mice inoculated subcutaneously with 1\u00d710^7 cells (Tumors developed within 21 days at 100% frequency (5\/5)).\uff08\u88f8\u9f20\u9ad8\u6210\u7624\u80fd\u529b\uff0c\u6210\u7624\u5468\u671f\u77ed\uff0821 \u5929\uff09\u3001\u6210\u7624\u7387 100%\uff0c\u63a5\u79cd\u5bc6\u5ea6\u4e0e NCI-H226 \u4e00\u81f4\uff1b\u6210\u7624\u75c5\u7406\u663e\u793a\u5178\u578b\u9cde\u764c\u7ed3\u6784\uff08\u89d2\u8d28\u5316\uff09\uff0c\u53ef\u4e0e NCI-H226 \u540c\u6b65\u5f00\u5c55\u88f8\u9f20\u6210\u7624\uff0c\u5bf9\u6bd4\u539f\u53d1\u4e0e\u8f6c\u79fb\u9cde\u764c\u7684\u4f53\u5185\u751f\u957f\u5dee\u5f02\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff1a\u2460p53 mRNA \u4f4e\u8868\u8fbe\uff08\u65e0 DNA \u7ed3\u6784\u5f02\u5e38\uff0c\u533a\u522b\u4e8e\u7a81\u53d8\u578b p53\uff09\uff1a\u53ef\u901a\u8fc7 qPCR \u68c0\u6d4b p53 mRNA \u6c34\u5e73\uff08\u5f15\u7269\uff1a\u6b63\u5411 5&#8242;-TGGAAGCCGGTTCAGGTGG-3&#8217;\uff0c\u53cd\u5411 5&#8242;-GGCCTTGGAACTCAAGGATG-3&#8217;\uff09\uff0c\u4e0e\u6b63\u5e38\u80ba\u7ec4\u7ec7\uff08HFL-1\uff09\u5bf9\u6bd4\u9a8c\u8bc1\uff1b\u2461\u89d2\u86cb\u767d + \u6ce2\u5f62\u86cb\u767d\u53cc\u9633\u6027\uff1a\u901a\u8fc7\u514d\u75ab\u8367\u5149\uff08\u89d2\u86cb\u767d\u4e00\u6297\uff1aab9377\uff0c\u6ce2\u5f62\u86cb\u767d\u4e00\u6297\uff1aab92547\uff09\u68c0\u6d4b\u53cc\u9633\u6027\u7ec6\u80de\u6bd4\u4f8b\uff08\u9884\u8ba1 30%-50%\uff09\uff0c\u5206\u6790 EMT \u503e\u5411\uff1b\u2462\u65e0\u795e\u7ecf\u4e1d\u4e09\u8054\u86cb\u767d\u8868\u8fbe\uff1a\u9a8c\u8bc1\u4e0a\u76ae\u6765\u6e90\uff0c\u6392\u9664\u795e\u7ecf\u5185\u5206\u6ccc\u5206\u5316\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7\u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001p53 mRNA \u68c0\u6d4b\uff08\u4f4e\u8868\u8fbe\uff09\u53ca\u53cc\u9633\u6027\u6807\u5fd7\u7269\u9a8c\u8bc1\uff0c\u786e\u4fdd\u6838\u5fc3\u7279\u6027\u7a33\u5b9a\uff1b\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53d1\u8d27\uff0c\u5efa\u8bae\u4e0e 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