{"id":218,"date":"2025-08-24T09:26:00","date_gmt":"2025-08-24T01:26:00","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=218"},"modified":"2025-08-24T09:26:00","modified_gmt":"2025-08-24T01:26:00","slug":"beas-2b%e4%ba%ba%e6%94%af%e6%b0%94%e7%ae%a1%e4%b8%8a%e7%9a%ae%e6%a0%b7%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/beas-2b%e4%ba%ba%e6%94%af%e6%b0%94%e7%ae%a1%e4%b8%8a%e7%9a%ae%e6%a0%b7%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"BEAS-2B\u4eba\u652f\u6c14\u7ba1\u4e0a\u76ae\u6837\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u540d\u79f0\uff1aBEAS-2B \u4eba\u652f\u6c14\u7ba1\u4e0a\u76ae\u6837\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff08\u4e0e SK-MES-1\uff08\u80ba\u9cde\u764c\u80f8\u6c34\u8f6c\u79fb\u300165 \u5c81\u7537\uff09\u3001HFL-1\uff08\u6b63\u5e38\u80ba\u6210\u7ea4\u7ef4\u3001\u80ce\u513f\u7537\uff09\u540c\u5c5e\u80ba\u6765\u6e90\u7ec6\u80de\uff0c\u533a\u522b\u4e8e PC-9\uff08\u80ba\u817a\u764c\uff09\u3001NCI-H520\uff08\u80ba\u9cde\u764c\u539f\u53d1\uff09\uff0c\u6838\u5fc3\u7279\u8272\u4e3a \u201c\u6b63\u5e38\u652f\u6c14\u7ba1\u4e0a\u76ae + \u817a\u75c5\u6bd2 12-SV40 \u8f6c\u5316 + \u751f\u7269\u5b89\u5168\u7b49\u7ea7 2 + \u4fdd\u7559\u5206\u5316\u80fd\u529b\u201d\uff0c\u662f\u80ba\u764c\u5fae\u73af\u5883\u7814\u7a76\u3001\u4e0a\u76ae\u5206\u5316\u673a\u5236\u3001\u6b63\u5e38 &#8211; \u80bf\u7624\u7ec6\u80de\u529f\u80fd\u5bf9\u6bd4\u7684\u5173\u952e\u6b63\u5e38\u5bf9\u7167\u6a21\u578b\uff1b\u53ef\u4e0e SK-MES-1\uff08\u80ba\u9cde\u764c\u8f6c\u79fb\uff09\u3001HFL-1\uff08\u6b63\u5e38\u80ba\u6210\u7ea4\u7ef4\uff09\u3001PC-9\uff08\u80ba\u817a\u764c\uff09\u7ec4\u6210 \u201c\u80ba\u7ec6\u80de &#8211; \u6b63\u5e38 \/ \u80bf\u7624\uff08\u4e0a\u76ae \/ \u6210\u7ea4\u7ef4\uff09+ \u7ec6\u80de\u7c7b\u578b\uff08\u652f\u6c14\u7ba1\u4e0a\u76ae \/ \u80ba\u6210\u7ea4\u7ef4\uff09+ \u6b63\u5e38\u5bf9\u7167\u201d \u4f53\u7cfb\uff0c\u63a2\u7d22\u80ba\u764c\u53d1\u751f\u4e2d\u652f\u6c14\u7ba1\u4e0a\u76ae\u7ec6\u80de\u7684\u8f6c\u5316\u673a\u5236\u53ca\u5fae\u73af\u5883\uff08\u4e0a\u76ae &#8211; \u6210\u7ea4\u7ef4\uff09\u76f8\u4e92\u4f5c\u7528\uff0c\u540c\u65f6\u56e0\u75c5\u6bd2\u8f6c\u5316\u7279\u6027\uff0c\u9002\u5408\u5f00\u5c55\u75c5\u6bd2\u81f4\u764c\u76f8\u5173\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u522b\u79f0\uff1aBeas-2B; BEAS 2B; BEAS2B; Beas2B; Bronchial Epithelium transformed with Ad12-SV40 2B\uff1b\u4eba\u652f\u6c14\u7ba1\u4e0a\u76ae\u6837\u7ec6\u80de\uff1b\u4eba\u652f\u6c14\u7ba1\u4e0a\u76ae\u6837\u7ec6\u80de\uff08\u522b\u79f0\u4ee5 \u201cBEAS-2B\u201d \u4e3a\u6838\u5fc3\uff0c\u201cBronchial Epithelium transformed with Ad12-SV40 2B\u201d \u660e\u786e\u6807\u6ce8 \u201c\u652f\u6c14\u7ba1\u4e0a\u76ae + \u75c5\u6bd2\u8f6c\u5316\u201d \u5c5e\u6027\uff0c\u4e0e \u201cSK-MES-1\u201d\u201cPC-9\u201d \u7b49\u80ba\u764c\u7ec6\u80de\u524d\u7f00\u5f62\u6210\u6e05\u6670\u533a\u5206\uff1b\u5927\u5c0f\u5199\u3001\u7a7a\u683c\u5dee\u5f02\u4e3a\u5e38\u89c1\u4e66\u5199\u53d8\u4f53\uff0c\u7edf\u4e00\u6807\u6ce8\u4fbf\u4e8e\u5b9e\u9a8c\u8bb0\u5f55\u68c0\u7d22\uff1b\u201c\u4eba\u652f\u6c14\u7ba1\u4e0a\u76ae\u6837\u7ec6\u80de\u201d \u7cbe\u51c6\u5b9a\u4f4d \u201c\u6b63\u5e38\u652f\u6c14\u7ba1 + \u4e0a\u76ae\u7ec6\u80de\u7c7b\u578b\u201d\uff0c\u907f\u514d\u4e0e\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08HFL-1\uff09\u3001\u80ba\u764c\u7ec6\u80de\uff08SK-MES-1\uff09\u6df7\u6dc6\uff0c\u8d34\u5408 \u201c\u6b63\u5e38\u80ba\u4e0a\u76ae\u7ec6\u80de + \u5b57\u6bcd\u6570\u5b57 + \u7ec6\u80de\u7c7b\u578b\u201d \u7684\u547d\u540d\u4f53\u7cfb\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u6027\u522b\u5e74\u9f84\uff1a\u672a\u660e\u786e\uff08STR \u4f4d\u70b9 Amelogenin\uff1aX,Y \u660e\u786e\u4e3a\u7537\u6027\u6838\u578b\uff0c\u5efa\u8bae\u7ed3\u5408\u4e34\u5e8a\u652f\u6c14\u7ba1\u4e0a\u76ae\u7ec6\u80de\u7814\u7a76\u5e38\u7528\u5e74\u9f84\uff08\u6210\u4eba\uff09\u63a8\u6d4b\u4e3a\u6210\u4eba\u7537\u6027\uff0c\u4e0e SK-MES-1\uff0865 \u5c81\u7537\u6210\u4eba\uff09\u5f62\u6210 \u201c\u6210\u4eba\u7537\u6b63\u5e38 &#8211; \u8001\u5e74\u7537\u80bf\u7624\u201d \u5e74\u9f84\u6027\u522b\u5bf9\u7167\uff0c\u4e0e HFL-1\uff08\u80ce\u513f\u7537\uff09\u5f62\u6210 \u201c\u6210\u4eba\u7537 &#8211; \u80ce\u513f\u7537\u201d \u5e74\u9f84\u68af\u5ea6\uff0c\u4e0e HT-29\uff0844 \u5c81\u5973\u6210\u4eba\u80a0\u764c\uff09\u5f62\u6210 \u201c\u6210\u4eba\u7537\u6b63\u5e38\u80ba &#8211; \u4e2d\u5e74\u5973\u80bf\u7624\u80a0\u201d \u6027\u522b\u5668\u5b98\u53cc\u91cd\u5bf9\u6bd4\uff1b\u6210\u4eba\u6765\u6e90\u9002\u5408\u6a21\u62df\u4e34\u5e8a\u6210\u4eba\u652f\u6c14\u7ba1\u4e0a\u76ae\u7684\u751f\u7406\u72b6\u6001\uff0c\u7528\u4e8e\u6210\u4eba\u80ba\u764c\u4e0e\u6b63\u5e38\u4e0a\u76ae\u7684\u5dee\u5f02\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec4\u7ec7\u6765\u6e90\uff1a\u80ba\uff1b\u5177\u4f53\u90e8\u4f4d\uff1a\u652f\u6c14\u7ba1\uff1b\u7ec6\u80de\u6027\u8d28\uff1a\u6b63\u5e38\uff1b\u7ec6\u80de\u7c7b\u578b\uff1a\u4e0a\u76ae\uff1b\u8f6c\u5316\u65b9\u5f0f\uff1a\u817a\u75c5\u6bd2 12-SV40 \u75c5\u6bd2\u6742\u4ea4\u75c5\u6bd2\u8f6c\u5316\uff08\u660e\u786e\u4e3a\u6b63\u5e38\u652f\u6c14\u7ba1\u4e0a\u76ae\u75c5\u6bd2\u8f6c\u5316\u6765\u6e90\uff0c\u4fdd\u7559\u6b63\u5e38\u4e0a\u76ae\u7279\u6709\u7684 \u201c\u9cde\u5173\u5206\u5316\u80fd\u529b\u3001\u4e0a\u76ae\u6807\u5fd7\u7269\u8868\u8fbe\u201d \u7279\u6027\uff0c\u540c\u65f6\u56e0\u75c5\u6bd2\u8f6c\u5316\u5177\u5907\u6c38\u751f\u5316\u7279\u5f81\uff08\u533a\u522b\u4e8e HFL-1 \u7684\u6709\u9650\u7ec6\u80de\u7cfb\uff09\uff1b\u533a\u522b\u4e8e SK-MES-1 \u7684\u80ba\u9cde\u764c\u80f8\u6c34\u8f6c\u79fb\u7076\u3001PC-9 \u7684\u80ba\u817a\u764c\uff0c\u53ef\u7528\u4e8e\u80ba\u764c\u53d1\u751f\u673a\u5236\u7814\u7a76\uff08\u5982\u75c5\u6bd2\u8f6c\u5316\u4e0e\u80ba\u764c\u7684\u5173\u8054\uff09\u3001\u652f\u6c14\u7ba1\u4e0a\u76ae\u5206\u5316\u529f\u80fd\u7814\u7a76\uff0c\u540c\u65f6\u4e3a\u4e34\u5e8a\u80ba\u764c\u8bca\u65ad\u6807\u5fd7\u7269\uff08\u5982\u4e0a\u76ae\u6807\u5fd7\u7269\uff09\u9a8c\u8bc1\u63d0\u4f9b\u6b63\u5e38\u5bf9\u7167\u4f9d\u636e\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\u751f\u957f\u6a21\u5f0f\u4e0e SK-MES-1\u3001PC-9 \u4e00\u81f4\uff0c\u4f46\u6838\u5fc3\u7279\u8272\u4e3a \u201c\u4e0a\u76ae\u7ec6\u80de\u6837\u8d34\u58c1 + \u75c5\u6bd2\u8f6c\u5316\u6c38\u751f\u5316 + \u4fdd\u7559\u5206\u5316\u80fd\u529b\u201d\uff1a\u2460\u589e\u6b96\u7279\u6027\uff1a10 \u4ee3\u4ee5\u5185\u7ec6\u80de\u589e\u6b96\u4e2d\u7b49\uff08\u7ed3\u5408\u75c5\u6bd2\u8f6c\u5316\u53ca\u6b63\u5e38\u4e0a\u76ae\u7279\u6027\uff0c\u63a8\u6d4b\u500d\u589e\u65f6\u95f4 28-36 \u5c0f\u65f6\uff0c\u5feb\u4e8e SK-MES-1 \u7684 50 \u5c0f\u65f6\uff0c\u6162\u4e8e PC-9 \u7684 35-50 \u5c0f\u65f6\uff09\uff0c\u56e0\u75c5\u6bd2\u8f6c\u5316\u5177\u5907\u6c38\u751f\u5316\u7279\u5f81\uff08\u65e0\u6709\u9650\u4f20\u4ee3\u9650\u5236\uff0c\u533a\u522b\u4e8e HFL-1 \u7684\u6709\u9650\u7ec6\u80de\u7cfb\uff09\uff1b\u2461\u8d34\u58c1\u7279\u6027\uff1a\u8d34\u58c1\u5f3a\u5ea6\u4f4e\u4e8e SK-MES-1\uff08\u9cde\u764c\u8f6c\u79fb\u7ec6\u80de\uff09\u3001\u9ad8\u4e8e PC-9\uff08\u817a\u764c\uff09\uff0c\u7ec6\u80de\u5448\u5178\u578b\u4e0a\u76ae\u6837\u94fa\u8def\u77f3\u6837\u6392\u5217\uff08\u652f\u6c14\u7ba1\u4e0a\u76ae\u7279\u5f81\uff0c\u65e0\u80bf\u7624\u7ec6\u80de\u7684\u4e0d\u89c4\u5219\u6392\u5217\uff09\uff1b\u5bf9\u6570\u751f\u957f\u671f\u4e3a\u63a5\u79cd\u540e 24-72 \u5c0f\u65f6\uff0c\u4f20\u4ee3\u9700\u6bcf 24 \u5c0f\u65f6\u955c\u68c0 1 \u6b21\uff0c\u5f85\u5bc6\u5ea6\u8fbe 80% \u65f6\u6309 1:3-1:4 \u4f20\u4ee3\uff08\u9ad8\u4e8e SK-MES-1 \u7684 1:2\uff09\uff0c\u4f20\u4ee3\u65f6\u9700\u907f\u514d\u8fc7\u5ea6\u6d88\u5316\uff08\u9632\u6b62\u7834\u574f\u4e0a\u76ae\u7ec6\u80de\u95f4\u8fde\u63a5\uff09\uff0c\u9002\u5408\u5f00\u5c55\u4e0a\u76ae\u5206\u5316\u3001\u75c5\u6bd2\u8f6c\u5316\u76f8\u5173\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u5f62\u6001\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u7ec6\u80de\u5448\u591a\u8fb9\u5f62\uff0c\u6392\u5217\u89c4\u6574\u5448\u94fa\u8def\u77f3\u6837\uff0c\u80de\u8d28\u4e30\u5bcc\uff0c\u65e0\u80bf\u7624\u7ec6\u80de\u7279\u6709\u7684\u89d2\u8d28\u5c0f\u4f53\u6216\u9ecf\u6db2\u9897\u7c92\uff0c\u6838\u6d46\u6bd4\u5c0f\u4e8e SK-MES-1\uff08\u9cde\u764c\uff09\uff0c\u4f53\u73b0\u6b63\u5e38\u4e0a\u76ae\u4e0e\u80bf\u7624\u7ec6\u80de\u7684\u5f62\u6001\u5dee\u5f02\uff1b\u4e0e SK-MES-1\uff08\u9cde\u764c\u4e0a\u76ae\u6837\u3001\u542b\u89d2\u8d28\u5c0f\u4f53\uff09\u76f8\u6bd4\uff0c\u6392\u5217\u66f4\u89c4\u6574\u4e14\u65e0\u80bf\u7624\u76f8\u5173\u5f62\u6001\u7279\u5f81\uff0c\u4e0e HFL-1\uff08\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\u3001\u68ad\u5f62\uff09\u76f8\u6bd4\uff0c\u5448\u5178\u578b\u4e0a\u76ae\u7ec6\u80de\u5f62\u6001\uff1b\u53ef\u901a\u8fc7 \u201c\u4e0a\u76ae\u6837 + SV40 \u6297\u539f\u9633\u6027 + \u65e0\u80bf\u7624\u5f62\u6001\u201d \u5feb\u901f\u9274\u522b\uff0c\u540c\u65f6\u4e0e OS-RC-2 \u80be\u764c\u7ec6\u80de\uff08\u4e0a\u76ae\u6837\uff09\u901a\u8fc7\u652f\u6c14\u7ba1\u4e0a\u76ae\u7279\u5f02\u6027\u6807\u5fd7\u7269\uff08\u5982 CK18\u3001TTF-1 \u4f4e\u8868\u8fbe\uff09\u8fdb\u4e00\u6b65\u533a\u5206\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4ee3\u6570\u7ec6\u80de\u80fd\u66f4\u597d\u4fdd\u7559 \u201c\u75c5\u6bd2\u8f6c\u5316\u7279\u6027\u3001\u9cde\u5173\u5206\u5316\u80fd\u529b\u3001\u6b63\u5e38\u4e0a\u76ae\u5f62\u6001\u201d \u6838\u5fc3\u7279\u5f81\uff0c\u5efa\u8bae\u4f20\u4ee3\u6b21\u6570\u4e0d\u8d85\u8fc7 15 \u4ee3\uff08\u591a\u4e8e HFL-1 \u7684 10 \u4ee3\uff0c\u56e0\u75c5\u6bd2\u8f6c\u5316\u6c38\u751f\u5316\uff09\uff0c\u9632\u6b62\u957f\u671f\u4f20\u4ee3\u5bfc\u81f4\u7279\u6027\u6f02\u79fb\uff0c\u5982\u5206\u5316\u80fd\u529b\u4e0b\u964d\uff08\u9cde\u5173\u5206\u5316\u9633\u6027\u7387\uff1c50%\uff09\u3001SV40 \u6297\u539f\u8868\u8fbe\u51cf\u5f31\u3001\u7ec6\u80de\u5f62\u6001\u5411\u68ad\u5f62\u8f6c\u53d8\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u80cc\u666f\u4ecb\u7ecd\uff1aBEAS-2B \u7ec6\u80de\u4ece\u975e\u764c\u4e2a\u4f53\u6b63\u5e38\u652f\u6c14\u7ba1\u4e0a\u76ae\u75c5\u7406\u5207\u7247\u5206\u79bb\uff0c\u7ecf\u817a\u75c5\u6bd2 12-SV40 \u75c5\u6bd2\u6742\u4ea4\u75c5\u6bd2\u611f\u67d3\u514b\u9686\u5efa\u7acb\uff0c\u6838\u5fc3\u7279\u6027\u53ca\u5e94\u7528\u5982\u4e0b\uff1a\u2460\u6b63\u5e38\u4e0e\u8f6c\u5316\u7279\u8272\uff1a\u4fdd\u7559\u6b63\u5e38\u652f\u6c14\u7ba1\u4e0a\u76ae\u7684\u9cde\u5173\u5206\u5316\u80fd\u529b\uff08\u53ef\u5bf9\u8840\u6e05\u53cd\u5e94\u8fdb\u884c\u5206\u5316\uff09\uff0c\u89d2\u86cb\u767d\uff08\u4e0a\u76ae\u6807\u5fd7\u7269\uff09\u53ca SV40 \u6297\u539f\uff08\u75c5\u6bd2\u8f6c\u5316\u6807\u5fd7\u7269\uff09\u53cc\u9633\u6027\uff0c\u662f\u7814\u7a76\u75c5\u6bd2\u8f6c\u5316\u4e0e\u4e0a\u76ae\u5206\u5316\u7684\u7406\u60f3\u6a21\u578b\uff1b\u2461\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u80ba\u764c\u5fae\u73af\u5883\u7814\u7a76\uff08\u5982\u4e0e SK-MES-1 \u5171\u57f9\u517b\u63a2\u7d22\u4e0a\u76ae &#8211; \u80bf\u7624\u76f8\u4e92\u4f5c\u7528\uff09\u3001\u652f\u6c14\u7ba1\u4e0a\u76ae\u5206\u5316\u673a\u5236\uff08\u5982\u8840\u6e05\u8bf1\u5bfc\u5206\u5316\uff09\u3001\u75c5\u6bd2\u81f4\u764c\u5b9e\u9a8c\uff08\u5982 Ad12-SV40 \u75c5\u6bd2\u5bf9\u4e0a\u76ae\u7ec6\u80de\u7684\u8f6c\u5316\u5f71\u54cd\uff09\uff0c\u540c\u65f6\u53ef\u4f5c\u4e3a\u80ba\u764c\u7814\u7a76\u7684\u6b63\u5e38\u4e0a\u76ae\u5bf9\u7167\uff0c\u8bc4\u4f30\u80bf\u7624\u7ec6\u80de\u4e0e\u6b63\u5e38\u4e0a\u76ae\u7684\u5206\u5b50\u5dee\u5f02\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u4f4d\u70b9\u4fe1\u606f\uff1aAmelogenin\uff1aX,Y\uff08\u660e\u786e\u7537\u6027\u6838\u578b\uff0c\u4e0e\u6027\u522b\u63a8\u6d4b\u4e00\u81f4\uff0c\u65e0\u6027\u522b\u77db\u76fe\u95ee\u9898\uff0c\u9a8c\u8bc1\u7ec6\u80de\u8eab\u4efd\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff0c\u4e0e SK-MES-1\uff08X,Y\uff09\u3001HFL-1\uff08\u63a8\u6d4b XY\uff09\u5f62\u6210\u6027\u522b\u6838\u578b\u5bf9\u7167\uff09; D8S1179\uff1a13, 15\uff1bD21S11\uff1a28, 30\uff08\u4e0e SK-MES-1 \u7684 29,30 \u5dee\u5f02\uff09\uff1bD7S820\uff1a10, 13\uff08\u4e0e SK-MES-1 \u7684 8 \u7eaf\u5408\u5b50\u5dee\u5f02\u663e\u8457\uff09\uff1bCSF1PO\uff1a9, 12\uff08\u4e0e SK-MES-1 \u7684 12 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bD3S1358\uff1a15, 17\uff08\u4e0e SK-MES-1 \u7684 16 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bTH01\uff1a7, 9.3\uff08\u4e0e SK-MES-1 \u4e00\u81f4\uff09\uff1bD13S317\uff1a13\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e SK-MES-1 \u7684 11 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bD16S539\uff1a12\uff08\u7eaf\u5408\u5b50\uff0c\u4e0e SK-MES-1 \u7684 13 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bD2S1338\uff1a22, 23\uff08\u4e0e SK-MES-1 \u7684 19 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bD19S433\uff1a13.2, 15.2\uff08\u7f55\u89c1\u7b49\u4f4d\u57fa\u56e0\uff0c\u6838\u5fc3\u9274\u522b\u4f4d\u70b9\uff09\uff1bvWA\uff1a17, 18\uff08\u4e0e SK-MES-1 \u7684 14 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bTHOX\uff1a6, 11\uff08\u63a8\u6d4b\u4e3a TPOX\uff0c\u4e0e SK-MES-1 \u7684 8 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bD18S51\uff1a18, 19\uff08\u4e0e SK-MES-1 \u7684 17 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bD5S818\uff1a12, 13\uff08\u4e0e SK-MES-1 \u7684 11 \u7eaf\u5408\u5b50\u5dee\u5f02\uff09\uff1bFGA\uff1a20, 24\uff08\u4e0e SK-MES-1 \u4e00\u81f4\uff09\uff1b\uff08STR \u4f4d\u70b9\u542b\u7f55\u89c1\u7b49\u4f4d\u57fa\u56e0\uff08D19S433\uff1a13.2,15.2\uff09\u53ca\u591a\u4e2a\u6742\u5408\u5b50\uff0c\u4e0e SK-MES-1\u3001PC-9 \u7684 STR \u56fe\u8c31\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7\u56fe\u8c31\u4e0e ATCC CRL-9609 \u6570\u636e\u6bd4\u5bf9\uff0c\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\u53ca\u6b63\u5e38\u652f\u6c14\u7ba1\u4e0a\u76ae\u5c5e\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a2\uff08\u9ad8\u4e8e SK-MES-1\u3001HFL-1\u3001PC-9 \u7684\u5b89\u5168\u7b49\u7ea7 1\uff0c\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e8c\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff08\u914d\u5907\u8d1f\u538b\u901a\u98ce\u3001\u4e2a\u4eba\u9632\u62a4\u88c5\u5907\uff08\u9632\u62a4\u670d\u3001\u62a4\u76ee\u955c\uff09\uff09\uff1b\u56e0\u542b\u817a\u75c5\u6bd2 12-SV40 \u75c5\u6bd2\u8f6c\u5316\u5e8f\u5217\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 60 \u5206\u949f\u5904\u7406\uff08\u706d\u6d3b\u75c5\u6bd2\u5e8f\u5217\uff09\uff0c\u907f\u514d\u75c5\u6bd2\u5e8f\u5217\u6269\u6563\uff1b\u5b9e\u9a8c\u64cd\u4f5c\u9700\u5728\u751f\u7269\u5b89\u5168\u67dc\u5185\u8fdb\u884c\uff0c\u9632\u6b62\u7ec6\u80de\u6c14\u6eb6\u80f6\u4f20\u64ad\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u4e0a\u76ae\u7ec6\u80de\u5448\u591a\u8fb9\u5f62\uff0c\u9700\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u56e2\u5757\u5f62\u6210\uff0c\u786e\u4fdd\u8ba1\u6570\u51c6\u786e\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226585%\uff08\u75c5\u6bd2\u8f6c\u5316\u7ec6\u80de\u590d\u82cf\u6d3b\u6027\u9ad8\uff09\uff0c\u4e14\u4e0a\u76ae\u6837\u5f62\u6001\u3001SV40 \u6297\u539f\u8868\u8fbe\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%-80%\uff08\u9ad8\u4e8e SK-MES-1 \u7684 60%-70%\uff09\uff0c\u6807\u6ce8 \u201c\u6b63\u5e38\u652f\u6c14\u7ba1\u4e0a\u76ae\u75c5\u6bd2\u8f6c\u5316\uff0c\u751f\u7269\u5b89\u5168\u7b49\u7ea7 2\uff0c\u4fdd\u7559\u5206\u5316\u80fd\u529b\u201d\uff0c\u63d0\u9192\u7528\u6237\u5173\u6ce8\u751f\u7269\u5b89\u5168\u9632\u62a4\u53ca\u5206\u5316\u7279\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u589e\u6b96\u3001\u5206\u5316\u80fd\u529b\u53ca SV40 \u6297\u539f\u8868\u8fbe\uff0c\u4fdd\u969c\u4e0e SK-MES-1\u3001HFL-1 \u5e73\u884c\u5b9e\u9a8c\uff08\u5982\u5171\u57f9\u517b\uff09\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CRL-9609\uff0cECACC; 95102433\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\uff08ATCC\u3001ECACC\uff09\u53cc\u91cd\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u6b63\u5e38\u652f\u6c14\u7ba1\u4e0a\u76ae\u7279\u6027\u3001\u75c5\u6bd2\u8f6c\u5316\u5c5e\u6027\u53ca\u5206\u5316\u80fd\u529b\u7ecf\u8fc7\u4e25\u683c\u9a8c\u8bc1\uff0c\u4e0e SK-MES-1 \u7684\u591a\u673a\u6784\u4fdd\u85cf\u4f53\u7cfb\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u673a\u6784\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a90% DMEM+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528 DMEM \u57f9\u517b\u57fa\uff0c\u4e0e SK-MES-1 \u7684 DMEM \u4e00\u81f4\uff0c\u533a\u522b\u4e8e HFL-1 \u7684 F12K\uff1bDMEM \u542b\u9ad8\u7cd6\uff084.5g\/L\uff09\u53ca\u9002\u914d\u652f\u6c14\u7ba1\u4e0a\u76ae\u7ec6\u80de\u7684\u8425\u517b\u914d\u6bd4\uff0c\u652f\u6301\u5176\u589e\u6b96\u3001\u5206\u5316\u80fd\u529b\u53ca SV40 \u6297\u539f\u5408\u6210\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u5206\u5316\u6291\u5236\u56e0\u5b50\u5f71\u54cd\u9cde\u5173\u5206\u5316\u80fd\u529b\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u75c5\u6bd2\u8f6c\u5316\u7279\u6027\u53ca\u4e0a\u76ae\u5f62\u6001\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e SK-MES-1\u3001HFL-1 \u7684\u5e38\u89c4 CO\u2082\u57f9\u517b\u6761\u4ef6\u4e00\u81f4\uff0c\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u56e0\u751f\u7269\u5b89\u5168\u7b49\u7ea7 2\uff0c\u57f9\u517b\u7bb1\u9700\u5b9a\u671f\u6d88\u6bd2\uff08\u5982\u7d2b\u5916\u7ebf\u7167\u5c04\uff09\uff0c\u907f\u514d\u4ea4\u53c9\u6c61\u67d3\uff1b\u4e0e\u5176\u4ed6\u80ba\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\u517c\u5bb9\u6027\u9ad8\uff0c\u4ec5\u9700\u9002\u914d\u751f\u7269\u5b89\u5168\u9632\u62a4\u8981\u6c42\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5f00\u5c55 \u201c\u6b63\u5e38 &#8211; \u80bf\u7624\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u4e0a\u76ae\u7ec6\u80de\u7279\u6027\u4e0e\u75c5\u6bd2\u8f6c\u5316\u5c5e\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 80%\uff0c\u5206\u5316\u80fd\u529b\u6700\u4f73\u3001SV40 \u6297\u539f\u8868\u8fbe\u7a33\u5b9a\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 2-3 \u5206\u949f\uff08\u77ed\u4e8e SK-MES-1 \u7684 3-4 \u5206\u949f\uff0c\u56e0\u8d34\u58c1\u5f3a\u5ea6\u7565\u4f4e\uff09\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u6536\u7f29\u53d8\u5706\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2\u00d710\u2076cells\/mL\uff08\u4e0e SK-MES-1 \u7684 2.5\u00d710\u2076cells\/mL \u76f8\u8fd1\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 DMEM \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\u4e8e\u751f\u7269\u5b89\u5168\u4e8c\u7ea7\u5b9e\u9a8c\u5ba4\u7684\u57f9\u517b\u7bb1\uff0c\u907f\u514d DMSO \u5bf9\u7ec6\u80de\u5206\u5316\u76f8\u5173\u86cb\u767d\uff08\u5982\u89d2\u86cb\u767d\uff09\u53ca SV40 \u6297\u539f\u7684\u5f71\u54cd\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u67d3\u8272\u4f53\uff1a47-48\uff08\u8fd1\u4e8c\u500d\u4f53\uff0c\u67d3\u8272\u4f53\u6570\u76ee\u7565\u9ad8\u4e8e\u6b63\u5e38\u4e8c\u500d\u4f53\uff0846 \u6761\uff09\uff0c\u4e0e SK-MES-1\uff08\u672a\u660e\u786e\uff09\u3001HFL-1\uff08\u6b63\u5e38\u4e8c\u500d\u4f53\u63a8\u6d4b\uff09\u5f62\u6210\u67d3\u8272\u4f53\u500d\u6027\u68af\u5ea6\uff1b\u8fd1\u4e8c\u500d\u4f53\u7279\u5f81\u7b26\u5408\u6b63\u5e38\u4e0a\u76ae\u7ec6\u80de\u7ecf\u75c5\u6bd2\u8f6c\u5316\u540e\u7684\u6838\u578b\u7279\u70b9\uff0c\u5b9e\u9a8c\u8bbe\u8ba1\u9700\u6ce8\u610f\u67d3\u8272\u4f53\u6570\u76ee\u5bf9\u57fa\u56e0\u8868\u8fbe\u68c0\u6d4b\u7684\u6f5c\u5728\u5f71\u54cd\uff0c\u9002\u5408\u5f00\u5c55\u6b63\u5e38\u4e0a\u76ae\u8f6c\u5316\u76f8\u5173\u7684\u6838\u578b\u7a33\u5b9a\u6027\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 DMEM \u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001SV40 \u6297\u539f\u68c0\u6d4b\uff08Western Blot \u9633\u6027\uff09\u53ca\u5206\u5316\u80fd\u529b\u9a8c\u8bc1\uff08\u9cde\u5173\u5206\u5316\u9633\u6027\uff09\uff0c\u786e\u4fdd\u6838\u5fc3\u7279\u6027\u7a33\u5b9a\uff1b\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u56e0\u751f\u7269\u5b89\u5168\u7b49\u7ea7 2\uff0c\u9700\u63d0\u9192\u7528\u6237\u63d0\u524d\u51c6\u5907\u751f\u7269\u5b89\u5168\u4e8c\u7ea7\u5b9e\u9a8c\u5ba4\u6761\u4ef6\uff0c\u907f\u514d\u5230\u8d27\u540e\u5ef6\u8bef\u57f9\u517b\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u751f\u7269\u5b89\u5168\u4e13\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u7528\u56fa\u5b9a\u652f\u67b6\u9632\u6b62\u57f9\u517b\u74f6\u6643\u52a8\uff0c\u51cf\u5c11\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u9700\u7acb\u5373\u5728\u751f\u7269\u5b89\u5168\u4e8c\u7ea7\u5b9e\u9a8c\u5ba4\u5f00\u7bb1\uff0c\u653e\u5165 CO\u2082\u57f9\u517b\u7bb1\uff0c24 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u72b6\u6001\uff0c48 \u5c0f\u65f6\u5185\u9996\u6b21\u6362\u6db2\uff0c\u786e\u4fdd\u7ec6\u80de\u5feb\u901f\u9002\u5e94\u73af\u5883\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u4e00\u822c\u914d\u5907 1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff1b\u51bb\u5b58\u7ba1\u9700\u5355\u72ec\u5305\u88c5\uff0c\u6807\u6ce8 \u201c\u751f\u7269\u5b89\u5168\u7b49\u7ea7 2\uff0c\u542b\u75c5\u6bd2\u8f6c\u5316\u5e8f\u5217\u201d\uff0c\u63d0\u9192\u8fd0\u8f93\u53ca\u63a5\u6536\u73af\u8282\u7684\u5b89\u5168\u9632\u62a4\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u6668\u8fbe\u670d\u52a1\uff0c\u6700\u5927\u9650\u5ea6\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca\u75c5\u6bd2\u8f6c\u5316\u7279\u6027\u6539\u53d8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e SK-MES-1\u3001HFL-1 \u7b49\u7ec6\u80de\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e25\u7981\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u3001\u7ec6\u80de\u6cbb\u7597\u3001\u7f8e\u5bb9\u53ca\u5176\u4ed6\u975e\u79d1\u7814\u7528\u9014\uff1b\u56e0\u542b\u817a\u75c5\u6bd2 12-SV40 \u75c5\u6bd2\u8f6c\u5316\u5e8f\u5217\uff0c\u9700\u9075\u5b88\u75c5\u6bd2\u76f8\u5173\u751f\u7269\u5b89\u5168\u6cd5\u89c4\uff0c\u5b9e\u9a8c\u524d\u9700\u63d0\u4ea4\u751f\u7269\u5b89\u5168\u5ba1\u67e5\u5907\u6848\uff0c\u786e\u4fdd\u5408\u89c4\u4f7f\u7528\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460\u751f\u7269\u5b89\u5168\u9632\u62a4\uff1a\u6240\u6709\u64cd\u4f5c\u9700\u5728\u751f\u7269\u5b89\u5168\u4e8c\u7ea7\u5b9e\u9a8c\u5ba4\u8fdb\u884c\uff0c\u5b9e\u9a8c\u4eba\u5458\u9700\u7a7f\u6234\u9632\u62a4\u670d\u3001\u62a4\u76ee\u955c\u3001\u624b\u5957\uff0c\u4f7f\u7528\u4e00\u6b21\u6027\u8017\u6750\uff08\u5982\u79fb\u6db2\u7ba1\u3001\u57f9\u517b\u74f6\uff09\uff0c\u5b9e\u9a8c\u540e\u8017\u6750\u53ca\u57f9\u517b\u57fa\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 60 \u5206\u949f\u5904\u7406\uff0c\u907f\u514d\u75c5\u6bd2\u5e8f\u5217\u6269\u6563\uff1b\u2461\u5206\u5316\u80fd\u529b\u5b9e\u9a8c\uff1a\u5f00\u5c55\u9cde\u5173\u5206\u5316\u5b9e\u9a8c\u65f6\uff0c\u53ef\u5c06\u7ec6\u80de\u63a5\u79cd\u4e8e\u542b 2% FBS \u7684 DMEM \u57f9\u517b\u57fa\uff08\u8bf1\u5bfc\u5206\u5316\uff09\uff0c\u57f9\u517b 72 \u5c0f\u65f6\u540e\u901a\u8fc7\u514d\u75ab\u8367\u5149\u68c0\u6d4b\u5206\u5316\u6807\u5fd7\u7269\uff08\u5982 CK13\uff0c\u9cde\u4e0a\u76ae\u5206\u5316\u6807\u5fd7\u7269\uff09\uff0c\u5bf9\u6bd4\u672a\u8bf1\u5bfc\u7ec4\uff0810% FBS\uff09\u7684\u8868\u8fbe\u5dee\u5f02\uff0c\u5206\u6790\u5206\u5316\u80fd\u529b\uff1b\u2462\u6b63\u5e38 &#8211; \u80bf\u7624\u5bf9\u6bd4\u5b9e\u9a8c\uff1a\u642d\u914d SK-MES-1\uff08\u80ba\u9cde\u764c\u8f6c\u79fb\uff09\u5f00\u5c55\u5b9e\u9a8c\u65f6\uff0c\u53ef\uff1aa. \u68c0\u6d4b\u4e0a\u76ae\u6807\u5fd7\u7269\uff08CK18\uff0cBEAS-2B \u9ad8\u8868\u8fbe\uff09\u3001\u80bf\u7624\u6807\u5fd7\u7269\uff08\u5982 MMP9\uff0cSK-MES-1 \u9ad8\u8868\u8fbe\uff09\u7684\u5dee\u5f02\uff0c\u9a8c\u8bc1\u6b63\u5e38\u4e0e\u80bf\u7624\u7ec6\u80de\u7279\u6027\uff1bb. \u5bf9\u6bd4\u4e24\u682a\u7ec6\u80de\u5bf9\u5316\u7597\u836f\uff08\u5982\u987a\u94c2\uff09\u7684 IC50 \u503c\uff08BEAS-2B \u4f5c\u4e3a\u6b63\u5e38\u7ec6\u80de IC50 \u66f4\u4f4e\uff0c\u5bf9\u836f\u7269\u66f4\u654f\u611f\uff09\uff0c\u8bc4\u4f30\u836f\u7269\u5bf9\u6b63\u5e38\u7ec6\u80de\u7684\u6bd2\u6027\uff1bc. \u68c0\u6d4b\u75c5\u6bd2\u8f6c\u5316\u76f8\u5173\u57fa\u56e0\uff08\u5982 SV40 T \u6297\u539f\uff0cBEAS-2B \u9633\u6027\uff09\u7684\u5dee\u5f02\uff0c\u5206\u6790\u75c5\u6bd2\u8f6c\u5316\u4e0e\u80bf\u7624\u53d1\u751f\u7684\u5173\u8054\uff1b\u2463\u957f\u671f\u57f9\u517b\u76d1\u6d4b\uff1a\u6bcf 5 \u4ee3\u68c0\u6d4b SV40 \u6297\u539f\u8868\u8fbe\uff08Western Blot\uff09\u3001\u5206\u5316\u80fd\u529b\uff08CK13 \u514d\u75ab\u8367\u5149\uff09\u53ca\u67d3\u8272\u4f53\u6570\u76ee\uff0847-48 \u6761\uff09\uff0c\u82e5\u53d1\u73b0 SV40 \u6297\u539f\u8868\u8fbe\u4e0b\u964d\u6216\u5206\u5316\u80fd\u529b\u51cf\u5f31\uff0c\u9700\u7acb\u5373\u66f4\u6362\u4f4e\u4ee3\u6570\u79cd\u5b50\u7ec6\u80de\uff1b\u2464\u57f9\u517b\u57fa\u9002\u914d\u6027\uff1a<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"23\" data-line=\"true\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aBEAS-2B \u4eba\u652f\u6c14\u7ba1\u4e0a\u76ae\u6837\u7ec6\u80de\uff08STR <\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/beas-2b%e4%ba%ba%e6%94%af%e6%b0%94%e7%ae%a1%e4%b8%8a%e7%9a%ae%e6%a0%b7%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        <\/div>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[17],"product_tag":[],"class_list":{"0":"post-218","1":"product","2":"type-product","3":"status-publish","5":"product_cat-17","7":"first","8":"instock","9":"sale","10":"taxable","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product\/218","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/comments?post=218"}],"wp:attachment":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/media?parent=218"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=218"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=218"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=218"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}