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\u901a\u8def\uff09\uff1b\u2461\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u9f3b\u54bd\u764c\u9ad8\u8f6c\u79fb\u673a\u5236\u7814\u7a76\uff08\u5982\u9f3b\u54bd\u764c\u7ec6\u80de\u4e0e\u95f4\u8d28\u7ec6\u80de\u7684\u76f8\u4e92\u4f5c\u7528\uff09\u3001\u6297\u8f6c\u79fb\u836f\u7269\u7b5b\u9009\uff08\u5982\u6291\u5236 MMPs \u6d3b\u6027\u7684\u836f\u7269\uff09\u3001\u8de8\u5668\u5b98\u8f6c\u79fb\u80bf\u7624\u5bf9\u6bd4\u5b9e\u9a8c\uff08\u4e0e C918\u3001SK-MES-1\uff09\uff0c\u540c\u65f6\u53ef\u4e0e\u4f4e\u8f6c\u79fb\u9f3b\u54bd\u764c\u7ec6\u80de\u7cfb\uff08\u5982 6-10B\uff09\u642d\u914d\u5f00\u5c55 \u201c\u8f6c\u79fb\u80fd\u529b\u5dee\u5f02\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff0c\u63a2\u7d22\u8f6c\u79fb\u76f8\u5173\u5206\u5b50\u6807\u5fd7\u7269\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u9274\u5b9a\uff1a\u672a\u660e\u786e\u5177\u4f53\u4f4d\u70b9\u4fe1\u606f\uff08\u5efa\u8bae\u53c2\u8003\u968f\u8d27 STR \u9274\u5b9a\u62a5\u544a\u6216\u901a\u8fc7 PCR \u68c0\u6d4b\u6838\u5fc3 STR \u4f4d\u70b9\uff08\u5982 Amelogenin\u3001CSF1PO\u3001D13S317 \u7b49\uff09\uff0c\u91cd\u70b9\u5173\u6ce8 Amelogenin \u57fa\u56e0\u4ee5\u786e\u8ba4\u6027\u522b\uff0c\u6392\u9664\u7ec6\u80de\u4ea4\u53c9\u6c61\u67d3\uff08\u5982\u4e0e SUNE-1 \u6bcd\u7cfb\u7ec6\u80de\u6216\u5176\u4ed6\u5668\u5b98\u80bf\u7624\u7ec6\u80de\u6df7\u6dc6\uff09\uff1b\u53ef\u5c06\u68c0\u6d4b\u7ed3\u679c\u4e0e C918\u3001SK-MES-1 \u7684 STR \u56fe\u8c31\u5bf9\u6bd4\uff0c\u660e\u786e 5-8F \u7684\u72ec\u7279\u4f4d\u70b9\uff0c\u786e\u4fdd\u5b9e\u9a8c\u7528\u7ec6\u80de\u8eab\u4efd\u51c6\u786e\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u53c2\u8003\u540c\u7c7b\u80bf\u7624\u7ec6\u80de\uff08C918\u3001SK-MES-1\uff09\u7684\u5b89\u5168\u7b49\u7ea7\uff0c\u9884\u8ba1\u4e3a 1 \u7ea7\uff0c\u4f4e\u4e8e BEAS-2B \u7684 2 \u7ea7\u3001HGC-27 \u7684 2 \u7ea7\uff1b\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u56e0\u662f\u9ad8\u8f6c\u79fb\u7ec6\u80de\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u907f\u514d\u7ec6\u80de\u6b8b\u7559\u6c61\u67d3\uff1b\u82e5\u6d89\u53ca EB \u75c5\u6bd2\u76f8\u5173\u5b9e\u9a8c\uff0c\u9700\u6309\u75c5\u6bd2\u5b9e\u9a8c\u9632\u62a4\u8981\u6c42\u64cd\u4f5c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u4e0a\u76ae\u7ec6\u80de\u5448\u591a\u8fb9\u5f62\uff0c\u9700\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u56e2\u5757\u5f62\u6210\uff0c\u786e\u4fdd\u8ba1\u6570\u51c6\u786e\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226585%\uff08\u9ad8\u8f6c\u79fb\u80bf\u7624\u7ec6\u80de\u590d\u82cf\u6d3b\u6027\u7a33\u5b9a\uff09\uff0c\u4e14\u4e0a\u76ae\u6837\u5f62\u6001\u3001\u9ad8\u8f6c\u79fb\u7279\u6027\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%-80%\uff08\u9ad8\u4e8e C918 \u7684 60%-70%\uff09\uff0c\u6807\u6ce8 \u201c\u4eba\u9ad8\u8f6c\u79fb\u9f3b\u54bd\u764c\u7ec6\u80de\u7cfb\uff0c\u5efa\u8bae\u68c0\u6d4b\u8f6c\u79fb\u76f8\u5173\u6807\u5fd7\u7269\u201d\uff0c\u63d0\u9192\u7528\u6237\u5229\u7528\u9ad8\u8f6c\u79fb\u7279\u6027\u5f00\u5c55\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u7ec6\u80de\u589e\u6b96\u3001\u8f6c\u79fb\u80fd\u529b\u53ca\u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\u7ed3\u679c\uff0c\u4fdd\u969c\u4e0e C918\u3001SK-MES-1 \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff08\u5efa\u8bae\u5b9e\u9a8c\u524d\u54a8\u8be2\u4f9b\u5e94\u5546\u83b7\u53d6\u4fdd\u85cf\u673a\u6784\u4fe1\u606f\uff08\u5982 ATCC\u3001ECACC 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\u542b\u9ad8\u7cd6\uff084.5g\/L\uff09\u53ca\u9002\u914d\u9ad8\u8f6c\u79fb\u9f3b\u54bd\u764c\u7ec6\u80de\u7684\u8425\u517b\u914d\u6bd4\uff0c\u652f\u6301\u5176\u5feb\u901f\u589e\u6b96\u53ca\u8f6c\u79fb\u76f8\u5173\u86cb\u767d\uff08\u5982 MMP2\u3001MMP9\uff09\u5408\u6210\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u8840\u6e05\u4e2d\u8f6c\u79fb\u6291\u5236\u56e0\u5b50\uff08\u5982\u67d0\u4e9b\u7ec6\u80de\u56e0\u5b50\uff09\u5f71\u54cd\u7ec6\u80de\u9ad8\u8f6c\u79fb\u7279\u6027\uff1b\u2462P\/S \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u7ec6\u80de\u9ad8\u8f6c\u79fb\u8868\u578b\u53ca\u4fb5\u88ad\u80fd\u529b\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e C918\u3001SK-MES-1 \u7684\u5e38\u89c4 CO\u2082\u57f9\u517b\u6761\u4ef6\u4e00\u81f4\uff0c\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u57f9\u517b\u7bb1\u5185\u907f\u514d\u5267\u70c8\u6643\u52a8\uff0c\u9632\u6b62\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u4e0e\u5176\u4ed6\u7ec6\u80de\u57f9\u517b\u6761\u4ef6\u517c\u5bb9\u6027\u9ad8\uff0c\u4ec5\u9700\u6ce8\u610f\u5355\u72ec\u653e\u7f6e\uff0c\u907f\u514d\u4ea4\u53c9\u6c61\u67d3\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5f00\u5c55 \u201c\u8de8\u5668\u5b98\u8f6c\u79fb\u80bf\u7624\u5bf9\u6bd4\u201d \u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u4e0a\u76ae\u7ec6\u80de\u5f62\u6001\u4e0e\u9ad8\u8f6c\u79fb\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 80%\uff0c\u8f6c\u79fb\u80fd\u529b\u6700\u4f73\u3001\u589e\u6b96\u6d3b\u6027\u7a33\u5b9a\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 2-3 \u5206\u949f\uff08\u77ed\u4e8e C918 \u7684 2-3 \u5206\u949f\uff0c\u56e0\u8d34\u58c1\u5f3a\u5ea6\u7565\u4f4e\uff09\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 3\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e C918 \u7684 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