{"id":224,"date":"2025-08-24T09:54:20","date_gmt":"2025-08-24T01:54:20","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=224"},"modified":"2025-08-24T09:54:20","modified_gmt":"2025-08-24T01:54:20","slug":"cne2%e4%ba%ba%e9%bc%bb%e5%92%bd%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89%ef%bc%88hela%e6%b1%a1%e6%9f%93%e7%bb%86%e8%83%9e%e7%b3%bb%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/cne2%e4%ba%ba%e9%bc%bb%e5%92%bd%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89%ef%bc%88hela%e6%b1%a1%e6%9f%93%e7%bb%86%e8%83%9e%e7%b3%bb%ef%bc%89\/","title":{"rendered":"CNE2\u4eba\u9f3b\u54bd\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09\uff08hela\u6c61\u67d3\u7ec6\u80de\u7cfb\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u540d\u79f0\uff1aCNE2 \u4eba\u9f3b\u54bd\u764c\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff08\u4e0e CNE1\uff08\u666e\u901a\u9f3b\u54bd\u764c\u7ec6\u80de\u3001STR \u6b63\u786e\uff09\u30015-8F\uff08\u9ad8\u8f6c\u79fb\u9f3b\u54bd\u764c\u7ec6\u80de\u3001\u7eaf\u51c0\uff09\u540c\u5c5e\u9f3b\u54bd\u6765\u6e90\u7eaf\u51c0\u80bf\u7624\u7ec6\u80de\uff0c\u533a\u522b\u4e8e CNE\uff08Hela \u6c61\u67d3\uff09\u3001MET-5A\uff08\u76ae\u80a4\u6b63\u5e38\u95f4\u76ae\uff09\uff0c\u6838\u5fc3\u7279\u8272\u4e3a \u201c\u9f3b\u6765\u6e90 + \u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u72b6\u7ec6\u80de\u764c + DMEM \u57f9\u517b\u57fa + STR \u9274\u5b9a\u6b63\u786e + \u73b0\u8d27\u4f9b\u5e94\u201d\uff0c\u662f\u9f3b\u54bd\u764c\u5206\u5316\u673a\u5236\u3001\u4f4e\u5206\u5316\u80bf\u7624\u6076\u6027\u7279\u6027\u7814\u7a76\u7684\u5173\u952e\u7eaf\u51c0\u6a21\u578b\uff1b\u53ef\u4e0e CNE1\uff08\u672a\u660e\u786e\u5206\u5316\uff09\u30015-8F\uff08\u9ad8\u8f6c\u79fb\uff09\u3001HFL-1\uff08\u6b63\u5e38\u80ba\u6210\u7ea4\u7ef4\uff09\u7ec4\u6210 \u201c\u9f3b\u54bd\u764c\u7ec6\u80de &#8211; \u4e0d\u540c\u5206\u5316\u7a0b\u5ea6 \/ \u8f6c\u79fb\u80fd\u529b + \u6b63\u5e38\u5bf9\u7167\u201d \u4f53\u7cfb\uff0c\u63a2\u7d22\u4f4e\u5206\u5316\u4e0e\u80bf\u7624\u6076\u6027\u7a0b\u5ea6\uff08\u5982\u4fb5\u88ad\u3001\u8010\u836f\uff09\u7684\u5173\u8054\u53ca\u57f9\u517b\u57fa\u5dee\u5f02\u5bf9\u9f3b\u54bd\u764c\u7ec6\u80de\u7684\u5f71\u54cd\uff0c\u540c\u65f6\u56e0\u4f4e\u5206\u5316\u7279\u6027\uff0c\u9002\u5408\u5f00\u5c55\u9f3b\u54bd\u764c\u4f4e\u5206\u5316\u76f8\u5173\u6807\u5fd7\u7269\u9a8c\u8bc1\u53ca\u6297\u4f4e\u5206\u5316\u80bf\u7624\u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u522b\u79f0\uff1aCNE2; \u4eba\u9f3b\u54bd\u764c\u7ec6\u80de\uff08\u522b\u79f0\u4ee5 \u201cCNE2\u201d \u4e3a\u6838\u5fc3\uff0c\u660e\u786e\u533a\u5206\u4e8e \u201cCNE1\u201d\u201cCNE\uff08\u6c61\u67d3\uff09\u201d\uff0c\u6807\u6ce8 \u201cSTR \u9274\u5b9a\u6b63\u786e\u201d \u5f3a\u8c03\u7eaf\u51c0\u5c5e\u6027\uff0c\u8865\u5145 \u201c\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u72b6\u7ec6\u80de\u764c\u201d \u7ec6\u5316\u75c5\u7406\u4e9a\u578b\uff1b\u201c\u4eba\u9f3b\u54bd\u764c\u7ec6\u80de\u201d \u7cbe\u51c6\u5b9a\u4f4d\u5668\u5b98\u4e0e\u80bf\u7624\u7c7b\u578b\uff0c\u8d34\u5408 \u201c\u4f4e\u5206\u5316\u7eaf\u51c0\u9f3b\u54bd\u764c\u7ec6\u80de + \u5b57\u6bcd\u6570\u5b57 + \u9274\u5b9a\u72b6\u6001\u201d \u7684\u547d\u540d\u4f53\u7cfb\uff0c\u907f\u514d\u4e0e\u540c\u7c7b\u7ec6\u80de\u7cfb\u6df7\u6dc6\uff0c\u540c\u65f6\u4e0e CNE1 \u7684 \u201c\u666e\u901a\u9f3b\u54bd\u764c\u201d\u30015-8F \u7684 \u201c\u9ad8\u8f6c\u79fb\u9f3b\u54bd\u764c\u201d \u5f62\u6210 \u201c\u5206\u5316\u7a0b\u5ea6 \/ \u8f6c\u79fb\u80fd\u529b\u201d \u53cc\u91cd\u4e9a\u578b\u533a\u5206\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u79cd\u5c5e\u6765\u6e90\uff1a\u4eba\uff08STR \u9274\u5b9a\u786e\u8ba4\u65e0\u4ea4\u53c9\u6c61\u67d3\uff0c\u7ec6\u80de\u7fa4\u4f53\u4e3a\u5355\u4e00\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u72b6\u7ec6\u80de\u764c\u7ec6\u80de\u7cfb\uff0c\u79cd\u5c5e\u7eaf\u51c0\u4e14\u6765\u6e90\u660e\u786e\uff0c\u4e0e CNE \u7684 \u201c\u4eba + HeLa \u6df7\u5408\u201d \u5f62\u6210\u672c\u8d28\u5dee\u5f02\uff0c\u53ef\u7a33\u5b9a\u8868\u73b0\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u7279\u6709\u7684\u751f\u7269\u5b66\u7279\u6027\uff08\u5982\u9ad8\u6076\u6027\u3001\u4f4e\u5206\u5316\u6807\u5fd7\u7269\u8868\u8fbe\uff09\uff0c\u65e0\u5916\u6e90\u7ec6\u80de\u5e72\u6270\uff0c\u5b9e\u9a8c\u7ed3\u679c\u91cd\u590d\u6027\u53ca\u53ef\u9760\u6027\u663e\u8457\u4f18\u4e8e\u6c61\u67d3\u7ec6\u80de\u7cfb\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u6027\u522b\u5e74\u9f84\uff1a\u672a\u660e\u786e\uff08\u5efa\u8bae\u5b9e\u9a8c\u524d\u901a\u8fc7 STR \u4f4d\u70b9 Amelogenin \u57fa\u56e0\uff08\u5df2\u9274\u5b9a\u53ef\u8ffd\u6eaf\uff09\u6216 SRY \u57fa\u56e0 PCR \u9a8c\u8bc1\u6027\u522b\uff0c\u53c2\u8003\u4e34\u5e8a\u4f4e\u5206\u5316\u9f3b\u54bd\u764c\u9ad8\u53d1\u5e74\u9f84\uff0840-60 \u5c81\uff09\u53ca\u7ec6\u80de\u6076\u6027\u7279\u6027\uff0c\u63a8\u6d4b\u4e3a\u4e2d\u5e74\u81f3\u4e2d\u8001\u5e74\u4e2a\u4f53\uff0c\u4e0e CNE1\uff08\u4e2d\u5e74\u81f3\u4e2d\u8001\u5e74\u63a8\u6d4b\uff09\u5f62\u6210 \u201c\u540c\u5e74\u9f84\u5c42\u4e0d\u540c\u5206\u5316\u7a0b\u5ea6\u201d \u5bf9\u7167\uff0c\u4e0e 5-8F\uff08\u4e2d\u5e74\u63a8\u6d4b\uff09\u5f62\u6210 \u201c\u540c\u5e74\u9f84\u5c42\u4e0d\u540c\u8f6c\u79fb\u80fd\u529b \/ \u5206\u5316\u7a0b\u5ea6\u201d \u5bf9\u7167\uff0c\u4e0e CNE\uff0858 \u5c81\u5973\u6c61\u67d3\uff09\u5f62\u6210 \u201c\u540c\u9f84\u5c42\u7eaf\u51c0\u4f4e\u5206\u5316 &#8211; \u6c61\u67d3\u201d \u5bf9\u6bd4\uff1b\u5e74\u9f84\u80cc\u666f\u9002\u914d\u4f4e\u5206\u5316\u9f3b\u54bd\u764c\u9ad8\u53d1\u4eba\u7fa4\u7814\u7a76\uff0c\u9002\u5408\u5f00\u5c55\u5e74\u9f84\u4e0e\u80bf\u7624\u5206\u5316\u7a0b\u5ea6\u5173\u8054\u5206\u6790\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec4\u7ec7\u6765\u6e90\uff1a\u9f3b\uff1b\u75c5\u7406\u4e9a\u578b\uff1a\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u72b6\u7ec6\u80de\u764c\uff08\u660e\u786e\u4e3a\u9f3b\u90e8\u4f4d\u6765\u6e90\uff0c\u4e14\u7ec6\u5316\u4e3a \u201c\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u201d\uff0c\u8f83 CNE1 \u7684 \u201c\u9f3b\u201d \u66f4\u7cbe\u51c6\uff0c\u63a8\u6d4b\u4e3a\u9f3b\u54bd\u90e8\u539f\u53d1\u7076\uff0c\u4fdd\u7559\u4f4e\u5206\u5316\u9cde\u764c\u7279\u6709\u7684 \u201c\u4f4e\u5206\u5316\u5f62\u6001\u3001\u9ad8\u4fb5\u88ad\u6f5c\u80fd\u3001\u9cde\u72b6\u4e0a\u76ae\u5206\u5316\u6b8b\u7559\u7279\u5f81\u201d\uff0c\u65e0 CNE1 \u7684\u672a\u660e\u786e\u5206\u5316\u7279\u6027\u53ca HeLa \u7684\u5bab\u9888\u6765\u6e90\u5c5e\u6027\uff1b\u533a\u522b\u4e8e CNE1 \u7684\u672a\u660e\u786e\u5206\u5316\u30015-8F \u7684\u9f3b\u54bd\u9ad8\u8f6c\u79fb\u7076\uff0c\u53ef\u7528\u4e8e\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u539f\u53d1\u673a\u5236\u7814\u7a76\uff08\u5982\u9f3b\u54bd\u9ecf\u819c\u4f4e\u5206\u5316\u764c\u53d8\u8fc7\u7a0b\uff09\u3001\u4e0d\u540c\u5206\u5316\u7a0b\u5ea6\u9f3b\u54bd\u764c\uff08CNE2 \u4f4e\u5206\u5316 vs CNE1 \u672a\u660e\u786e\u5206\u5316\uff09\u7684\u5206\u5b50\u5dee\u5f02\u5bf9\u6bd4\uff0c\u540c\u65f6\u4e3a\u4e34\u5e8a\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u539f\u53d1\u7076\u8bca\u7597\u65b9\u6848\u4f18\u5316\u63d0\u4f9b\u7eaf\u51c0\u5b9e\u9a8c\u4f9d\u636e\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u957f\u7279\u6027\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\u751f\u957f\u6a21\u5f0f\u4e0e CNE1\u30015-8F \u4e00\u81f4\uff0c\u4f46\u6838\u5fc3\u7279\u8272\u4e3a \u201c\u4f4e\u5206\u5316\u4e0a\u76ae\u6837\u8d34\u58c1 + \u9ad8\u6076\u6027\u589e\u6b96\u7279\u5f81\u201d\uff1a\u2460\u589e\u6b96\u7279\u6027\uff1a10 \u4ee3\u4ee5\u5185\u7ec6\u80de\u589e\u6b96\u901f\u5ea6\u5feb\uff0c\u7ed3\u5408\u4f4e\u5206\u5316\u80bf\u7624\u5e38\u89c4\u89c4\u5f8b\uff0c\u63a8\u6d4b\u500d\u589e\u65f6\u95f4 26-36 \u5c0f\u65f6\uff08\u5feb\u4e8e CNE1 \u7684 30-40 \u5c0f\u65f6\uff0c\u4e0e 5-8F \u7684 24-32 \u5c0f\u65f6\u76f8\u8fd1\uff09\uff0c\u65e0\u6709\u9650\u4f20\u4ee3\u9650\u5236\uff08\u6c38\u751f\u5316\u4f4e\u5206\u5316\u80bf\u7624\u7ec6\u80de\uff0c\u533a\u522b\u4e8e HFL-1 \u7684\u6709\u9650\u7ec6\u80de\u7cfb\uff09\uff0c\u4e14\u589e\u6b96\u5747\u4e00\u6027\u9ad8\uff08\u65e0 CNE \u7684\u5f02\u8d28\u6027\uff09\uff0c\u56e0\u4f4e\u5206\u5316\u7279\u6027\uff0c\u589e\u6b96\u8fc7\u7a0b\u4e2d\u6613\u7ef4\u6301\u4f4e\u5206\u5316\u72b6\u6001\uff1b\u2461\u8d34\u58c1\u7279\u6027\uff1a\u8d34\u58c1\u5f3a\u5ea6\u4ecb\u4e8e CNE1\uff08\u8d34\u58c1\u4e2d\u7b49\uff09\u4e0e 5-8F\uff08\u9ad8\u8f6c\u79fb\uff0c\u8d34\u58c1\u4e2d\u7b49\uff09\u4e4b\u95f4\uff0c\u7ec6\u80de\u5448\u4f4e\u5206\u5316\u4e0a\u76ae\u6837\u5f62\u6001\uff08\u6392\u5217\u8f83 CNE1 \u677e\u6563\uff0c\u4f53\u73b0\u4f4e\u5206\u5316\u7279\u5f81\uff09\uff0c\u5bf9\u6570\u751f\u957f\u671f\u4e3a\u63a5\u79cd\u540e 24-60 \u5c0f\u65f6\uff0c\u4f20\u4ee3\u9700\u6bcf 24 \u5c0f\u65f6\u955c\u68c0 1 \u6b21\uff0c\u5f85\u5bc6\u5ea6\u8fbe 75% \u65f6\u6309 1:3-1:4 \u4f20\u4ee3\uff08\u9ad8\u4e8e CNE1 \u7684 1:2-1:3\uff09\uff0c\u4f20\u4ee3\u8fc7\u7a0b\u65e0\u6c61\u67d3\u7ec6\u80de\u8fc7\u5ea6\u751f\u957f\u98ce\u9669\uff0c\u53ef\u7a33\u5b9a\u7ef4\u6301\u4f4e\u5206\u5316\u7279\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u5f62\u6001\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u7ec6\u80de\u5448\u591a\u8fb9\u5f62\uff0c\u6392\u5217\u8f83\u677e\u6563\uff08\u4f53\u73b0\u4f4e\u5206\u5316\u7279\u5f81\uff09\uff0c\u80de\u8d28\u4e30\u5bcc\uff0c\u6838\u6d46\u6bd4\u5927\u4e8e CNE1\uff08\u672a\u660e\u786e\u5206\u5316\uff09\uff0c\u6838\u4ec1\u660e\u663e\uff0c\u65e0 CNE \u7684\u5f62\u6001\u5f02\u8d28\u6027\u53ca HeLa \u7684\u677e\u6563\u5bab\u9888\u764c\u7ec6\u80de\u7279\u5f81\uff0c\u4f53\u73b0\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u7684\u5178\u578b\u5f62\u6001\uff1b\u4e0e CNE1\uff08\u89c4\u6574\u4e0a\u76ae\u6837\u3001\u6392\u5217\u7d27\u5bc6\uff09\u76f8\u6bd4\uff0c\u6392\u5217\u66f4\u677e\u6563\u4e14\u6838\u6d46\u6bd4\u66f4\u9ad8\uff08\u4f4e\u5206\u5316\u6807\u5fd7\uff09\uff0c\u4e0e 5-8F\uff08\u7d27\u5bc6\u4e0a\u76ae\u6837\u3001\u9ad8\u8f6c\u79fb\u7279\u5f81\uff09\u76f8\u6bd4\uff0c\u65e0\u9ad8\u8f6c\u79fb\u7ec6\u80de\u7684\u7d27\u5bc6\u6392\u5217\uff0c\u4e14\u5206\u5316\u7a0b\u5ea6\u66f4\u4f4e\uff1b\u53ef\u901a\u8fc7 \u201c\u4e0a\u76ae\u6837 + \u4f4e\u5206\u5316\u9cde\u764c\u7279\u5f02\u6027\u6807\u5fd7\u7269\uff08CK5\/6 \u9ad8\u8868\u8fbe\u3001CK19 \u4f4e\u8868\u8fbe\uff09\u9633\u6027\u201d \u5feb\u901f\u9274\u522b\uff0c\u4ec5\u9700\u5355\u4e00\u6807\u5fd7\u7269\u7ec4\u5408\u68c0\u6d4b\u5373\u53ef\u786e\u8ba4\u8eab\u4efd\u53ca\u5206\u5316\u7a0b\u5ea6\uff0c\u65e0\u9700\u50cf CNE \u90a3\u6837\u8fdb\u884c\u6c61\u67d3\u6392\u67e5\uff0c\u5b9e\u9a8c\u64cd\u4f5c\u66f4\u9ad8\u6548\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4ee3\u6570\u7ec6\u80de\u80fd\u66f4\u597d\u4fdd\u7559 \u201c\u4f4e\u5206\u5316\u5c5e\u6027\u3001\u4e0a\u76ae\u5f62\u6001\u3001\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u6838\u5fc3\u7279\u5f81\u201d\uff0c\u5efa\u8bae\u4f20\u4ee3\u6b21\u6570\u4e25\u683c\u63a7\u5236\u5728 10 \u4ee3\u4ee5\u5185\uff0c\u9632\u6b62\u957f\u671f\u4f20\u4ee3\u5bfc\u81f4\u7279\u6027\u6f02\u79fb\uff08\u5982\u5206\u5316\u7a0b\u5ea6\u5347\u9ad8\u3001\u4f4e\u5206\u5316\u6807\u5fd7\u7269\uff08\u5982 CK5\/6\uff09\u8868\u8fbe\u4e0b\u964d\u3001\u589e\u6b96\u901f\u5ea6\u51cf\u6162\uff09\uff0c\u56e0\u65e0\u6c61\u67d3\uff0c\u4f20\u4ee3\u8fc7\u7a0b\u4e2d\u7ec6\u80de\u7fa4\u4f53\u59cb\u7ec8\u4fdd\u6301\u5355\u4e00\u4f4e\u5206\u5316\u72b6\u6001\uff0c\u65e0\u9700\u50cf CNE \u90a3\u6837\u76d1\u6d4b\u6c61\u67d3\u6bd4\u4f8b\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u80cc\u666f\u4ecb\u7ecd\uff1aCNE2 \u7ec6\u80de\u7cfb\u4e3a\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u72b6\u7ec6\u80de\u764c\u7ec6\u80de\u7cfb\uff0c\u6838\u5fc3\u7279\u6027\u53ca\u5e94\u7528\u5982\u4e0b\uff1a\u2460\u4f4e\u5206\u5316\u7279\u8272\uff1a\u5177\u5907\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u7279\u6709\u7684\u751f\u7269\u5b66\u7279\u6027\uff0c\u5982\u9ad8\u4fb5\u88ad\u6027\u3001\u4f4e\u5206\u5316\u6807\u5fd7\u7269\uff08CK5\/6\u3001p63\uff09\u9ad8\u8868\u8fbe\u3001\u9ad8\u8010\u836f\u6f5c\u80fd\uff0c\u662f\u7814\u7a76\u4f4e\u5206\u5316\u9f3b\u54bd\u764c\u6076\u6027\u673a\u5236\u7684\u7406\u60f3\u6a21\u578b\uff1b\u2461\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u57fa\u7840\u673a\u5236\u7814\u7a76\uff08\u5982\u4f4e\u5206\u5316\u8c03\u63a7\u901a\u8def\u3001\u9cde\u72b6\u4e0a\u76ae\u5206\u5316\u5f02\u5e38\uff09\u3001\u4e0d\u540c\u5206\u5316\u7a0b\u5ea6\u9f3b\u54bd\u764c\u5bf9\u6bd4\u5b9e\u9a8c\uff08\u4e0e CNE1\uff09\u3001\u6297\u4f4e\u5206\u5316\u80bf\u7624\u836f\u7269\u7b5b\u9009\uff08\u5982\u9488\u5bf9\u4f4e\u5206\u5316\u764c\u7ec6\u80de\u7684\u5316\u7597\u836f\u3001\u9776\u5411\u836f\uff09\uff0c\u540c\u65f6\u53ef\u4e0e 5-8F \u642d\u914d\u5f00\u5c55 \u201c\u4f4e\u5206\u5316 vs \u9ad8\u8f6c\u79fb\u201d \u9f3b\u54bd\u764c\u53cc\u91cd\u7279\u6027\u5bf9\u6bd4\u5b9e\u9a8c\uff0c\u63a2\u7d22\u5206\u5316\u7a0b\u5ea6\u4e0e\u8f6c\u79fb\u80fd\u529b\u7684\u5173\u8054\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u9274\u5b9a\uff1aSTR \u9274\u5b9a\u6b63\u786e\uff08\u6838\u5fc3\u4fdd\u969c\uff1a\u2460\u901a\u8fc7\u81f3\u5c11 16 \u4e2a\u6838\u5fc3 STR \u4f4d\u70b9\u68c0\u6d4b\uff08\u5982 Amelogenin\u3001CSF1PO\u3001D13S317 \u7b49\uff09\uff0c\u7ed3\u679c\u4e0e\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u7ec6\u80de\u6807\u51c6\u56fe\u8c31\u4e00\u81f4\uff0c\u65e0 HeLa \u7ec6\u80de STR \u4f4d\u70b9\u7279\u5f81\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff1b\u2461\u53ef\u901a\u8fc7\u5bf9\u6bd4\u56fd\u5185\u9f3b\u54bd\u764c\u7ec6\u80de\u5e93\uff08\u5982\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u7ec6\u80de\u5e93\uff09\u7684\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u7ec6\u80de STR \u6570\u636e\uff0c\u8fdb\u4e00\u6b65\u9a8c\u8bc1\u8eab\u4efd\u53ca\u5206\u5316\u7a0b\u5ea6\u5173\u8054\u7279\u5f81\uff0c\u4e0e CNE \u7684 \u201c\u6c61\u67d3\u65e0\u6cd5\u901a\u8fc7\u9274\u5b9a\u201d \u5f62\u6210\u9c9c\u660e\u5bf9\u6bd4\uff0c\u786e\u4fdd\u5b9e\u9a8c\u7528\u7ec6\u80de\u8eab\u4efd\u7cbe\u51c6\u4e14\u5206\u5316\u7279\u6027\u660e\u786e\uff0c\u907f\u514d\u56e0\u5206\u5316\u7a0b\u5ea6\u6df7\u6dc6\u6216\u6c61\u67d3\u5bfc\u81f4\u5b9e\u9a8c\u7ed3\u8bba\u504f\u5dee\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e CNE1\u30015-8F \u7684\u5b89\u5168\u7b49\u7ea7\u4e00\u81f4\uff0c\u4f4e\u4e8e BEAS-2B \u7684 2 \u7ea7\uff1b\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u56e0\u7ec6\u80de\u4e3a\u4f4e\u5206\u5316\u9ad8\u6076\u6027\u80bf\u7624\u7ec6\u80de\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u907f\u514d\u7ec6\u80de\u6b8b\u7559\u6c61\u67d3\uff1b\u56e0\u7ec6\u80de\u7eaf\u51c0\uff0c\u65e0\u9700\u50cf CNE \u90a3\u6837\u5355\u72ec\u4f7f\u7528\u4e13\u7528\u8017\u6750\uff0c\u4ec5\u9700\u5e38\u89c4\u65e0\u83cc\u64cd\u4f5c\u5373\u53ef\uff0c\u964d\u4f4e\u64cd\u4f5c\u590d\u6742\u5ea6\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u56e0\u4f4e\u5206\u5316\u4e0a\u76ae\u7ec6\u80de\u6392\u5217\u677e\u6563\uff0c\u8f7b\u67d4\u5439\u6253\u5373\u53ef\u83b7\u5f97\u5355\u7ec6\u80de\u60ac\u6db2\uff0c\u8ba1\u6570\u51c6\u786e\u6027\u9ad8\uff08\u8bef\u5dee\uff1c5%\uff09\uff0c\u65e0 CNE \u7684\u6bd4\u4f8b\u8ba1\u7b97\u95ee\u9898\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u7ecf\u68c0\u6d4b\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226585%\uff08\u4f4e\u5206\u5316\u80bf\u7624\u7ec6\u80de\u590d\u82cf\u6d3b\u6027\u7a33\u5b9a\uff09\uff0c\u4e14\u4f4e\u5206\u5316\u4e0a\u76ae\u5f62\u6001\u3001\u6076\u6027\u7279\u6027\u65e0\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%-75%\uff0c\u6807\u6ce8 \u201cSTR \u9274\u5b9a\u6b63\u786e\uff0c\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\uff0cDMEM \u57f9\u517b\u57fa\uff0c\u5efa\u8bae\u68c0\u6d4b\u4f4e\u5206\u5316\u6807\u5fd7\u7269\u201d\uff0c\u63d0\u9192\u7528\u6237\u5229\u7528\u4f4e\u5206\u5316\u7279\u6027\u5f00\u5c55\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u7ed3\u5408 STR \u9274\u5b9a\u6b63\u786e\uff0c\u5b9e\u73b0 \u201c\u5fae\u751f\u7269\u6c61\u67d3 &#8211; \u7ec6\u80de\u4ea4\u53c9\u6c61\u67d3\u201d \u53cc\u91cd\u65e0\u98ce\u9669\uff0c\u5b9e\u9a8c\u53ef\u9760\u6027\u8fdc\u9ad8\u4e8e CNE\uff08\u4ec5\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff09\uff0c\u4fdd\u969c\u4e0e CNE1\u30015-8F \u5e73\u884c\u5b9e\u9a8c\u7684\u4e00\u81f4\u6027\uff0c\u5c24\u5176\u9002\u5408\u4f4e\u5206\u5316\u4e0e\u5176\u4ed6\u5206\u5316\u7a0b\u5ea6\u9f3b\u54bd\u764c\u7684\u5bf9\u6bd4\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff08\u5efa\u8bae\u5b9e\u9a8c\u524d\u54a8\u8be2\u4f9b\u5e94\u5546\u83b7\u53d6\u4fdd\u85cf\u673a\u6784\u4fe1\u606f\uff08\u5982\u56fd\u5185\u9f3b\u54bd\u764c\u7ec6\u80de\u5e93\u3001ECACC\uff09\uff0c\u56e0 STR \u9274\u5b9a\u6b63\u786e\uff0c\u53ef\u901a\u8fc7\u4fdd\u85cf\u673a\u6784\u8ffd\u6eaf\u7ec6\u80de\u539f\u59cb\u80cc\u666f\uff08\u5982\u6027\u522b\u5e74\u9f84\u3001\u5206\u5316\u7a0b\u5ea6\u9a8c\u8bc1\u6570\u636e\uff09\uff0c\u8865\u5145\u5b8c\u5584\u4fe1\u606f\uff0c\u8fdb\u4e00\u6b65\u63d0\u5347\u5b9e\u9a8c\u53ef\u8ffd\u6eaf\u6027\uff0c\u4e0e CNE \u7684 \u201c\u65e0\u6743\u5a01\u4fdd\u85cf\u201d \u5f62\u6210\u5dee\u5f02\uff0c\u540c\u65f6\u4e3a\u4f4e\u5206\u5316\u7279\u6027\u63d0\u4f9b\u7b2c\u4e09\u65b9\u9a8c\u8bc1\u4f9d\u636e\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a90% DMEM+10% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff1a\u2460\u91c7\u7528 DMEM \u57f9\u517b\u57fa\uff0c\u4e0e CNE1 \u7684 RPMI-1640 \u5f62\u6210\u663e\u8457\u5dee\u5f02\uff0c\u542b\u9ad8\u7cd6\uff084.5g\/L\uff09\u53ca\u9002\u914d\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u7ec6\u80de\u7684\u8425\u517b\u914d\u6bd4\uff0c\u652f\u6301\u5176\u9ad8\u589e\u6b96\u901f\u5ea6\u53ca\u4f4e\u5206\u5316\u76f8\u5173\u86cb\u767d\uff08\u5982 CK5\/6\uff09\u5408\u6210\uff0c\u65e0\u9700\u50cf CNE \u90a3\u6837\u9002\u914d\u6df7\u5408\u7ec6\u80de\uff1b\u246110% FBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff0c\u907f\u514d\u5f71\u54cd\u7ec6\u80de\u4f4e\u5206\u5316\u72b6\u6001\u53ca\u589e\u6b96\u5747\u4e00\u6027\uff0c\u65e0\u9700\u8003\u8651\u6c61\u67d3\u7ec6\u80de\u7684\u8425\u517b\u9700\u6c42\uff1b\u2462PS \u7ec8\u6d53\u5ea6\u4e3a 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff0c\u6709\u6548\u9884\u9632\u7ec6\u83cc\u6c61\u67d3\uff0c\u4e0d\u5f71\u54cd\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\u7279\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e CNE1\u30015-8F \u7684\u5e38\u89c4 CO\u2082\u57f9\u517b\u6761\u4ef6\u4e00\u81f4\uff0c\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff0c\u5b9a\u671f\u66f4\u6362\u6c34\u76d8\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u6e17\u900f\u538b\u5347\u9ad8\uff1b\u56e0\u7ec6\u80de\u7eaf\u51c0\u4e14\u4e3a\u4f4e\u5206\u5316\u7279\u6027\uff0c\u57f9\u517b\u7bb1\u5185\u53ef\u4e0e CNE1\u30015-8F \u7b49\u7eaf\u51c0\u7ec6\u80de\u5171\u540c\u653e\u7f6e\uff0c\u65e0\u9700\u50cf CNE \u90a3\u6837\u5355\u72ec\u9694\u79bb\uff0c\u5b9e\u9a8c\u7a7a\u95f4\u5229\u7528\u66f4\u9ad8\u6548\uff0c\u540c\u65f6\u4fbf\u4e8e\u5f00\u5c55\u591a\u7ec6\u80de\u7cfb\u5e73\u884c\u5bf9\u6bd4\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u4f4e\u5206\u5316\u4e0a\u76ae\u7ec6\u80de\u7279\u6027\uff1a\u2460\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 75%\uff0c\u4f4e\u5206\u5316\u72b6\u6001\u7a33\u5b9a\u3001\u6d3b\u6027\u6700\u4f73\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 2-3 \u5206\u949f\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff08\u4f4e\u5206\u5316\u7ec6\u80de\u80de\u8d28\u8f83\u8106\u5f31\uff09\uff1b\u24611000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2.5\u00d710\u2076cells\/mL\uff08\u4e0e 5-8F \u76f8\u8fd1\uff0c\u9ad8\u4e8e CNE1 \u7684 2\u00d710\u2076cells\/mL\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684 DMEM \u57f9\u517b\u57fa\u91cd\u60ac\uff0c\u76f4\u63a5\u63a5\u79cd\uff0c\u65e0 CNE \u7684 \u201c\u6c61\u67d3\u7ec6\u80de\u4f18\u5148\u751f\u957f\u201d \u95ee\u9898\uff0c\u4e14\u4f4e\u5206\u5316\u7279\u6027\u6062\u590d\u901f\u5ea6\u5feb\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 DMEM \u57f9\u517b\u57fa\u9002\u5e94\u6027\u57f9\u517b\u3001STR \u9274\u5b9a\u590d\u6838\u3001\u652f\u539f\u4f53\u68c0\u6d4b\u53ca\u4f4e\u5206\u5316\u7279\u6027\u9a8c\u8bc1\uff08\u5982 CK5\/6 \u9ad8\u8868\u8fbe\uff09\uff0c\u6838\u5fc3\u7279\u6027\u7a33\u5b9a\uff0c\u4e0e CNE \u7684 \u201c\u6682\u4e0d\u4f9b\u5e94\u201d \u5f62\u6210\u9c9c\u660e\u5bf9\u6bd4\uff1b\u4e0b\u5355\u540e 48 \u5c0f\u65f6\u5185\u53ef\u5b89\u6392\u53d1\u8d27\uff0c\u53ef\u4e0e CNE1\uff081 \u5468\u8d27\u671f\uff09\u30015-8F\uff081 \u5468\u8d27\u671f\uff09\u540c\u6b65\u4e0b\u5355\uff0c\u4fdd\u969c \u201c\u4e0d\u540c\u5206\u5316\u7a0b\u5ea6 \/ \u8f6c\u79fb\u80fd\u529b\u9f3b\u54bd\u764c\u7ec6\u80de\u201d \u5e73\u884c\u5b9e\u9a8c\u7684\u65f6\u95f4\u4e00\u81f4\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u7528\u56fa\u5b9a\u652f\u67b6\u9632\u6b62\u57f9\u517b\u74f6\u6643\u52a8\uff0c\u51cf\u5c11\u4f4e\u5206\u5316\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff08\u4f4e\u5206\u5316\u7ec6\u80de\u8d34\u58c1\u7565\u8106\u5f31\uff09\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u653e\u5165 5% CO\u2082\u57f9\u517b\u7bb1\uff0c24 \u5c0f\u65f6\u540e\u89c2\u5bdf\u7ec6\u80de\u8d34\u58c1\u72b6\u6001\uff0c48 \u5c0f\u65f6\u5185\u9996\u6b21\u6362\u6db2\uff0c\u65e0\u9700\u50cf CNE \u90a3\u6837\u62c5\u5fc3\u8fd0\u8f93\u540e\u6c61\u67d3\u6bd4\u4f8b\u53d8\u5316\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u914d\u5907 1kg \u5e72\u51b0\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff0c\u4fdd\u969c\u4f4e\u5206\u5316\u7279\u6027\u65e0\u6539\u53d8\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u6668\u8fbe\u670d\u52a1\uff0c\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u907f\u514d\u4f4e\u5206\u5316\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca\u7279\u6027\u6539\u53d8\uff0c\u65e0\u9700\u50cf CNE \u90a3\u6837\u62c5\u5fe7\u8fd0\u8f93\u52a0\u5267\u6c61\u67d3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e CNE1\u30015-8F \u7b49\u7ec6\u80de\u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4f46\u56e0\u7ec6\u80de\u4e3a\u4f4e\u5206\u5316\u7eaf\u51c0\u6a21\u578b\uff0c\u53ef\u7528\u4e8e\u53d1\u8868\u4f4e\u5206\u5316\u9f3b\u54bd\u764c\u76f8\u5173\u9ad8\u8d28\u91cf\u79d1\u7814\u6210\u679c\uff0c\u65e0 CNE \u7684 \u201c\u5b9e\u9a8c\u7ed3\u8bba\u4e0d\u53ef\u9760\u201d \u98ce\u9669\uff0c\u9002\u5408\u5f00\u5c55\u9700\u660e\u786e\u5206\u5316\u7a0b\u5ea6\u7684\u4e25\u8c28\u5b9e\u9a8c\uff08\u5982\u5206\u5316\u8c03\u63a7\u673a\u5236\u3001\u4f4e\u5206\u5316\u80bf\u7624\u9776\u5411\u6cbb\u7597\uff09\uff09<\/li>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u6838\u5fc3\u5efa\u8bae\uff1a\u2460\u4f4e\u5206\u5316\u7279\u6027\u5229\u7528\uff1a\u5f00\u5c55\u4f4e\u5206\u5316\u9f3b\u54bd\u764c\u5b9e\u9a8c\u65f6\uff0c\u53ef\u91cd\u70b9\u68c0\u6d4b\u4f4e\u5206\u5316 \/ \u9cde\u764c\u6807\u5fd7\u7269\uff08CK5\/6\u3001p63\u3001EGFR\uff09\uff0c\u901a\u8fc7 Western Blot \u6216\u514d\u75ab\u8367\u5149\u9a8c\u8bc1\u5176\u8868\u8fbe\uff08\u9884\u8ba1 CK5\/6\u3001p63 \u9ad8\u8868\u8fbe\uff09\uff0c\u4e0e CNE1 \u5bf9\u6bd4\u5206\u6790\u5206\u5316\u7a0b\u5ea6\u5dee\u5f02\uff1b\u5f00\u5c55\u836f\u7269\u7b5b\u9009\u65f6\uff0c\u53ef\u9009\u62e9\u9488\u5bf9\u4f4e\u5206\u5316\u80bf\u7624\u7684\u836f\u7269\uff08\u5982\u987a\u94c2\u3001\u5409\u897f\u4ed6\u6ee8\uff09\uff0c\u8bbe\u7f6e\u6d53\u5ea6\u68af\u5ea6\u68c0\u6d4b IC50\uff0c\u9a8c\u8bc1\u4f4e\u5206\u5316\u7ec6\u80de\u7684\u8010\u836f\u7279\u6027\uff1b\u2461\u4e0e\u4e0d\u540c\u5206\u5316 \/ \u8f6c\u79fb\u80fd\u529b\u7ec6\u80de\u5bf9\u6bd4\uff1a\u642d\u914d CNE1\uff08\u672a\u660e\u786e\u5206\u5316\uff09\u30015-8F\uff08\u9ad8\u8f6c\u79fb\uff09\u5f00\u5c55\u5b9e\u9a8c\u65f6\uff0c\u53ef\uff1aa. \u68c0\u6d4b\u5206\u5316\u76f8\u5173\u57fa\u56e0\uff08\u5982 SOX2\u3001p63\uff0cCNE2 \u9ad8\u8868\u8fbe\uff09\u3001\u8f6c\u79fb\u76f8\u5173\u57fa\u56e0\uff08\u5982 MMP2\u3001CXCR4\uff0c5-8F \u9ad8\u8868\u8fbe\uff09\u7684\u5dee\u5f02\uff0c\u5206\u6790\u5206\u5316\u7a0b\u5ea6\u4e0e\u8f6c\u79fb\u80fd\u529b\u7684\u5173\u8054\uff1bb. \u5bf9\u6bd4\u4e09\u682a\u7ec6\u80de\u7684\u4fb5\u88ad\u80fd\u529b\uff08Transwell \u7a7f\u819c\u6570\uff1a5-8F\uff1eCNE2\uff1eCNE1\uff09\uff0c\u9a8c\u8bc1\u4f4e\u5206\u5316\u4e0e\u9ad8\u4fb5\u88ad\u7684\u76f8\u5173\u6027\uff1bc. \u68c0\u6d4b\u57f9\u517b\u57fa\u9002\u5e94\u6027\uff08CNE2 DMEM vs CNE1 RPMI-1640\uff09\uff0c\u5206\u6790\u57f9\u517b\u57fa\u5bf9\u4e0d\u540c\u5206\u5316\u7a0b\u5ea6\u9f3b\u54bd\u764c\u7ec6\u80de\u589e\u6b96\u7684\u5f71\u54cd\uff1b\u2462\u957f\u671f\u57f9\u517b\u76d1\u6d4b\uff1a\u6bcf 5 \u4ee3\u68c0\u6d4b STR \u4f4d\u70b9\uff08\u6838\u5fc3\u4f4d\u70b9\u5373\u53ef\uff09\u3001\u7ec6\u80de\u5f62\u6001\uff08\u662f\u5426\u7ef4\u6301\u4f4e\u5206\u5316\u4e0a\u76ae\u6837\u677e\u6563\u6392\u5217\uff09\u3001\u4f4e\u5206\u5316\u6807\u5fd7\u7269\u8868\u8fbe\uff08CK5\/6\u3001p63\uff09\u53ca\u589e\u6b96\u901f\u5ea6\uff0c\u786e\u4fdd\u4f4e\u5206\u5316\u5c5e\u6027\u65e0\u6539\u53d8\uff0c\u65e0\u9700\u50cf CNE \u90a3\u6837\u9891\u7e41\u8fdb\u884c\u5168\u9762\u6c61\u67d3\u68c0\u6d4b\uff1b\u2463\u66ff\u4ee3\u65b9\u6848\u63d0\u793a\uff1a\u82e5\u7814\u7a76\u4f4e\u5206\u5316\u9f3b\u54bd\u9cde\u764c\uff0c\u5efa\u8bae\u4f18\u5148\u9009\u62e9 CNE2\uff08STR \u6b63\u786e\u3001\u4f4e\u5206\u5316\u660e\u786e\uff09\uff0c\u907f\u514d\u4f7f\u7528 CNE1\uff08\u5206\u5316\u672a\u660e\u786e\uff09\u6216 CNE\uff08\u6c61\u67d3\uff09\uff0c\u540c\u65f6\u53ef\u901a\u8fc7 CNE2\uff08\u4f4e\u5206\u5316\uff09+ CNE1\uff08\u672a\u660e\u786e\u5206\u5316\uff09+ 5-8F\uff08\u9ad8\u8f6c\u79fb\uff09\u7684\u7ec4\u5408\uff0c\u6784\u5efa\u9f3b\u54bd\u764c \u201c\u5206\u5316\u7a0b\u5ea6 &#8211; \u8f6c\u79fb\u80fd\u529b\u201d \u7814\u7a76\u4f53\u7cfb\uff0c\u8986\u76d6\u66f4\u591a\u4e34\u5e8a\u573a\u666f\uff1b\u2464\u57f9\u517b\u57fa\u6ce8\u610f\u4e8b\u9879\uff1a\u56e0\u4f7f\u7528 DMEM \u57f9\u517b\u57fa\uff0c\u4e0e CNE1 \u7684 RPMI-1640 \u4e0d\u540c\uff0c\u5b9e\u9a8c\u4e2d\u82e5\u9700\u5bf9\u6bd4\u4e24\u682a\u7ec6\u80de\uff0c\u9700\u5206\u522b\u4f7f\u7528\u5bf9\u5e94\u9002\u914d\u57f9\u517b\u57fa\uff0c\u4e0d\u53ef\u6df7\u7528\uff0c\u907f\u514d\u56e0\u57f9\u517b\u57fa\u5dee\u5f02\u5e72\u6270\u5206\u5316\u7a0b\u5ea6\u6216\u589e\u6b96\u5bf9\u6bd4\u7ed3\u679c\u3002<\/li>\n<\/ul>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aCNE2 \u4eba\u9f3b\u54bd\u764c\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff08<\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/cne2%e4%ba%ba%e9%bc%bb%e5%92%bd%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89%ef%bc%88hela%e6%b1%a1%e6%9f%93%e7%bb%86%e8%83%9e%e7%b3%bb%ef%bc%89\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        <\/div>\n","protected":false},"featured_media":0,"comment_status":"open","ping_status":"closed","template":"","meta":[],"product_brand":[],"product_cat":[17],"product_tag":[],"class_list":{"0":"post-224","1":"product","2":"type-product","3":"status-publish","5":"product_cat-17","7":"first","8":"instock","9":"sale","10":"taxable","11":"shipping-taxable","12":"purchasable","13":"product-type-simple"},"jetpack_sharing_enabled":true,"_links":{"self":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product\/224","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/types\/product"}],"replies":[{"embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/comments?post=224"}],"wp:attachment":[{"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/media?parent=224"}],"wp:term":[{"taxonomy":"product_brand","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_brand?post=224"},{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_cat?post=224"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/www.fengyaosw.com\/index.php\/wp-json\/wp\/v2\/product_tag?post=224"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}