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\u8131\u843d\u8c03\u63a7\u673a\u5236\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u5f62\u6001\uff1a\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u8d34\u58c1\u7ec6\u80de\u5448\u591a\u8fb9\u5f62\uff0c\u6392\u5217\u677e\u6563\u5448\u94fa\u8def\u77f3\u6837\uff0c\u80de\u8d28\u4e30\u5bcc\uff1b\u60ac\u6d6e\u7ec6\u80de\u5448\u5c0f\u7c07\u72b6\uff083-5 \u4e2a\u7ec6\u80de\u805a\u96c6\uff09\uff0c\u5f62\u6001\u4e0e\u8d34\u58c1\u7ec6\u80de\u4e00\u81f4\uff0c\u65e0 JF-305 \u7684\u7d27\u5bc6\u6392\u5217\u3001HPAC \u7684\u5206\u6563\u751f\u957f\u7279\u5f81\uff1b\u6838\u6d46\u6bd4\u5927\u4e8e JF-305\uff08\u4e2d\u5e74\uff09\u3001\u5c0f\u4e8e HPAC\uff08\u540c\u9f84\u817a\u6ce1\u764c\uff09\uff0c\u4f53\u73b0\u8001\u5e74\u534a\u8d34\u58c1\u764c\u7ec6\u80de\u7684\u5f62\u6001\u7279\u5f81\uff1b\u53ef\u901a\u8fc7 \u201c\u4e0a\u76ae\u6837 + \u534a\u8d34\u58c1\u751f\u957f + CSF-I \u9633\u6027\u201d \u5feb\u901f\u9274\u522b\uff0c\u540c\u65f6\u4e0e JF-305 \u901a\u8fc7\u751f\u957f\u7279\u6027\uff08\u534a\u8d34\u58c1 vs \u8d34\u58c1\uff09\u3001\u4e0e HPAC \u901a\u8fc7\u75c5\u7406\u4e9a\u578b\u6807\u5fd7\u7269\uff08\u5982\u80f0\u86cb\u767d\u9176\u539f\u9634\u6027\uff09\u8fdb\u4e00\u6b65\u533a\u5206\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u4ee3\u6570\uff1a10 \u4ee3\u4ee5\u5185\uff08\u4f4e\u4ee3\u6570\u7ec6\u80de\u80fd\u66f4\u597d\u4fdd\u7559 \u201c\u534a\u8d34\u58c1\u7279\u6027\u3001\u53cc\u8840\u6e05\u4f9d\u8d56\u3001\u57fa\u56e0\u8868\u8fbe\u8c31\u201d \u6838\u5fc3\u5c5e\u6027\uff0c\u5efa\u8bae\u4f20\u4ee3\u6b21\u6570\u4e0d\u8d85\u8fc7 10 \u4ee3\uff0c\u9632\u6b62\u957f\u671f\u4f20\u4ee3\u5bfc\u81f4\u7279\u6027\u6f02\u79fb\uff0c\u5982\u8d34\u58c1\u7ec6\u80de\u5360\u6bd4\u4e0b\u964d\uff08\uff1c50%\uff09\u3001\u500d\u589e\u65f6\u95f4\u5ef6\u957f\uff08\uff1e45 \u5c0f\u65f6\uff09\u3001CSF-I \u8868\u8fbe\u964d\u4f4e\uff0c\u786e\u4fdd\u7ec6\u80de\u59cb\u7ec8\u8d34\u5408\u534a\u8d34\u58c1\u751f\u957f\u7684\u5b9e\u9a8c\u9700\u6c42\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u80cc\u666f\u7b80\u4ecb\uff1aMIA-PACA-2 \u7ec6\u80de 1975 \u5e74\u7531 A. Yunis \u7b49\u4ece 65 \u5c81\u767d\u4eba\u7537\u6027\u80f0\u817a\u80bf\u7624\u7ec4\u7ec7\u5efa\u7cfb\uff0c\u6838\u5fc3\u7279\u6027\u53ca\u5e94\u7528\u5982\u4e0b\uff1a\u2460\u751f\u957f\u7279\u6027\u4ef7\u503c\uff1a\u4f5c\u4e3a\u7f55\u89c1\u534a\u8d34\u58c1\u751f\u957f\u80f0\u817a\u764c\u7ec6\u80de\u7cfb\uff0c\u53ef\u7528\u4e8e\u7814\u7a76\u7ec6\u80de\u9ecf\u9644\u5206\u5b50\uff08\u5982 E-cadherin\uff09\u8868\u8fbe\u4e0e\u8d34\u58c1\u80fd\u529b\u7684\u5173\u8054\uff0c\u63a2\u7d22\u80bf\u7624\u7ec6\u80de \u201c\u4e0a\u76ae &#8211; \u95f4\u8d28\u8f6c\u5316\u201d \u8fc7\u7a0b\u4e2d\u7684\u534a\u60ac\u6d6e\u72b6\u6001\u673a\u5236\uff1b\u2461\u53cc\u8840\u6e05\u7279\u8272\uff1a\u9700\u540c\u65f6\u6dfb\u52a0 FBS \u4e0e\u9a6c\u8840\u6e05\u624d\u80fd\u7ef4\u6301\u6700\u4f73\u751f\u957f\uff0c\u9002\u5408\u5f00\u5c55\u8840\u6e05\u6210\u5206\u5bf9\u80bf\u7624\u7ec6\u80de\u751f\u957f\u7684\u8c03\u63a7\u7814\u7a76\uff08\u5982\u9a6c\u8840\u6e05\u4e2d\u7684\u672a\u77e5\u56e0\u5b50\u534f\u540c FBS \u4fc3\u8fdb\u589e\u6b96\uff09\uff1b\u2462\u5e94\u7528\u573a\u666f\uff1a\u5e7f\u6cdb\u7528\u4e8e\u8001\u5e74\u80f0\u817a\u764c\u57fa\u7840\u673a\u5236\u3001\u534a\u8d34\u58c1\u80bf\u7624\u7ec6\u80de\u836f\u7269\u7b5b\u9009\uff08\u9700\u540c\u65f6\u8003\u8651\u8d34\u58c1\u4e0e\u60ac\u6d6e\u7ec6\u80de\u7684\u836f\u7269\u654f\u611f\u6027\uff09\u3001CSF-I \u76f8\u5173\u80bf\u7624\u5fae\u73af\u5883\u7814\u7a76\uff0c\u540c\u65f6\u53ef\u4f5c\u4e3a \u201c\u53cc\u8840\u6e05\u57f9\u517b\u201d \u7684\u9633\u6027\u5bf9\u7167\uff0c\u4f18\u5316\u5176\u4ed6\u96be\u57f9\u517b\u80bf\u7624\u7ec6\u80de\u7684\u57f9\u517b\u57fa\u914d\u65b9\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u9274\u5b9a\uff1aSTR \u9274\u5b9a\u901a\u8fc7\uff08\u6838\u5fc3\u4fdd\u969c\uff1a\u2460\u901a\u8fc7\u81f3\u5c11 16 \u4e2a\u6838\u5fc3 STR \u4f4d\u70b9\u68c0\u6d4b\uff08\u5982 Amelogenin\u3001CSF1PO\u3001D13S317 \u7b49\uff09\uff0c\u7ed3\u679c\u4e0e ATCC CRL-1420\u3001DSMZ ACC-733 \u6807\u51c6\u56fe\u8c31\u4e00\u81f4\uff0cAmelogenin\uff1aY\uff08\u786e\u8ba4\u7537\u6027\u6838\u578b\uff0c\u4e0e\u6027\u522b\u6807\u6ce8\u4e00\u81f4\uff09\uff0c\u65e0 JF-305\u3001HPAC \u7684 STR \u4f4d\u70b9\u7279\u5f81\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff1b\u2461\u53ef\u901a\u8fc7\u5bf9\u6bd4\u591a\u673a\u6784\uff08ATCC\/DSMZ\/ECACC\uff09\u7684 STR \u6570\u636e\uff0c\u9a8c\u8bc1\u7ec6\u80de\u8eab\u4efd\u7684\u4e00\u81f4\u6027\uff0c\u5c24\u5176\u9002\u5408\u8de8\u56fd\u534f\u4f5c\u7814\u7a76\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff0c\u907f\u514d\u56e0\u7ec6\u80de\u7cfb\u6df7\u6dc6\u5bfc\u81f4\u5b9e\u9a8c\u7ed3\u8bba\u504f\u5dee\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e JF-305\u3001HPAC \u4e00\u81f4\uff0c\u4f4e\u4e8e BEAS-2B \u7684 2 \u7ea7\uff1b\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u89c4\u8303\uff0c\u56e0\u542b\u534a\u60ac\u6d6e\u7ec6\u80de\uff0c\u6362\u6db2\u65f6\u9700\u8f7b\u67d4\u64cd\u4f5c\uff0c\u907f\u514d\u5c06\u8d34\u58c1\u7ec6\u80de\u51b2\u8131\uff1b\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u542b\u60ac\u6d6e\u7ec6\u80de\u7684\u5e9f\u6db2\u9700\u76f4\u63a5\u706d\u83cc\uff0c\u65e0\u9700\u989d\u5916\u79bb\u5fc3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u9700\u540c\u65f6\u6536\u96c6\u8d34\u58c1\u6d88\u5316\u7ec6\u80de\u4e0e\u60ac\u6d6e\u7ec6\u80de\uff0c\u5408\u5e76\u540e\u53f0\u76fc\u84dd\u67d3\u8272\u8ba1\u6570\uff0c\u786e\u4fdd\u8ba1\u6570\u8bef\u5dee\uff1c8%\uff08\u9ad8\u4e8e JF-305 \u7684 5%\uff09\uff1b\u51bb\u5b58\u7ba1\u542b 10% DMSO \u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226580%\uff08\u4f4e\u4e8e JF-305 \u7684 85%\uff09\uff0c\u4e14\u534a\u8d34\u58c1\u6bd4\u4f8b\uff08\u8d34\u58c1 70%-80%\uff09\u3001\u57fa\u56e0\u8868\u8fbe\u65e0\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u6807\u6ce8 \u201c\u80f0\u817a\u764c\u7ec6\u80de\uff0c\u534a\u8d34\u58c1\u751f\u957f\uff0c\u9700\u53cc\u8840\u6e05\u57f9\u517b\u57fa\uff0c65 \u5c81\u7537\u767d\u4eba\u201d\uff0c\u63d0\u9192\u7528\u6237\u5173\u6ce8\u7279\u6b8a\u751f\u957f\u7279\u6027\u53ca\u57f9\u517b\u914d\u65b9\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u6c61\u67d3\u53e0\u52a0\u534a\u8d34\u58c1\u7279\u6027\u5bfc\u81f4\u7684\u751f\u957f\u5f02\u5e38\uff0c\u4fdd\u969c\u4e0e JF-305\u3001HPAC \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff0c\u5c24\u5176\u9002\u5408\u591a\u7ec6\u80de\u7cfb\u751f\u957f\u7279\u6027\u5bf9\u6bd4\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1aATCC; CRL-1420\uff0cDSMZ; ACC-733\uff0cECACC; 85062806\uff0cJCRB; JCRB00\uff08\u56fd\u9645\u56db\u5927\u6743\u5a01\u673a\u6784\u4fdd\u85cf\uff0c\u7ec6\u80de\u6765\u6e90\u53ef\u8ffd\u6eaf\u6027\u6781\u5f3a\uff0c\u534a\u8d34\u58c1\u7279\u6027\u3001\u53cc\u8840\u6e05\u9700\u6c42\u7ecf\u8fc7\u591a\u673a\u6784\u9a8c\u8bc1\uff0c\u4e0e JF-305 \u7684\u672a\u660e\u786e\u4fdd\u85cf\u3001HPAC \u7684\u5355\u673a\u6784\u4fdd\u85cf\u5f62\u6210\u663e\u8457\u4f18\u52bf\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u534f\u4f5c\u53ca\u9ad8\u8d28\u91cf\u6587\u732e\u53d1\u8868\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a90% DMEM+10% FBS+2.5% \u9a6c\u8840\u6e05 + \u53cc\u6297\uff08\u6838\u5fc3\u7279\u8272\uff0c\u4e0e JF-305 \u5bf9\u6bd4\uff09\uff1a\u2460\u53cc\u8840\u6e05\u914d\u65b9\uff1a\u91c7\u7528 DMEM \u57f9\u517b\u57fa\uff08\u4e0e JF-305 \u7684 RPMI-1640 \u5dee\u5f02\uff09\uff0c\u9700\u540c\u65f6\u6dfb\u52a0 10% FBS \u4e0e 2.5% \u9a6c\u8840\u6e05\uff08\u7f3a\u4e00\u4e0d\u53ef\uff0c\u5b9e\u9a8c\u9a8c\u8bc1\uff1a\u7f3a\u5931\u9a6c\u8840\u6e05\u65f6\u7ec6\u80de\u589e\u6b96\u4e0b\u964d 50%\uff0c\u8d34\u58c1\u6bd4\u4f8b\u964d\u81f3 40%\uff09\uff0c\u9a6c\u8840\u6e05\u53ef\u63d0\u4f9b FBS \u4e2d\u7f3a\u4e4f\u7684\u9ecf\u9644\u76f8\u5173\u56e0\u5b50\uff08\u5982\u67d0\u4e9b\u7cd6\u86cb\u767d\uff09\uff0c\u652f\u6301\u534a\u8d34\u58c1\u751f\u957f\uff1b\u2461\u6210\u5206\u8981\u6c42\uff1aFBS \u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u6279\u6b21\uff08\u5357\u7f8e\u6765\u6e90\u4f18\u5148\uff09\uff0c\u9a6c\u8840\u6e05\u9700\u9009\u62e9\u900f\u6790\u578b\uff08\u53bb\u9664\u5c0f\u5206\u5b50\u5e72\u6270\u7269\u8d28\uff09\uff0c\u53cc\u6297\u7ec8\u6d53\u5ea6 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff1b\u57f9\u517b\u57fa\u9700\u5728 2-8\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u5f00\u5c01\u540e 1 \u5468\u5185\u4f7f\u7528\u5b8c\u6bd5\uff08\u9a6c\u8840\u6e05\u6210\u5206\u6613\u964d\u89e3\uff09\uff1b\u2462\u5e94\u7528\u4ef7\u503c\uff1a\u9002\u5408\u5f00\u5c55\u8840\u6e05\u6210\u5206\u7b5b\u9009\u5b9e\u9a8c\uff08\u5982 FBS \/ \u9a6c\u8840\u6e05\u6d53\u5ea6\u68af\u5ea6\u7ec4\u5408\uff09\uff0c\u63a2\u7d22\u4e0d\u540c\u8840\u6e05\u5bf9\u80bf\u7624\u7ec6\u80de\u8d34\u58c1\u80fd\u529b\u7684\u5f71\u54cd\uff0c\u540c\u65f6\u4e3a\u5176\u4ed6\u534a\u8d34\u58c1\u80bf\u7624\u7ec6\u80de\u7684\u57f9\u517b\u57fa\u4f18\u5316\u63d0\u4f9b\u53c2\u8003\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e JF-305\u3001HPAC \u4e00\u81f4\uff0c\u6e7f\u5ea6\u9700\u7ef4\u6301\u5728 75%-85%\uff08\u9ad8\u4e8e JF-305 \u7684 70%-80%\uff09\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u8840\u6e05\u6d53\u5ea6\u5f02\u5e38\uff1b\u57f9\u517b\u7bb1\u9700\u7a33\u5b9a\u63a7\u6e29\uff08\u6ce2\u52a8\u2264\u00b10.5\u2103\uff09\uff0c\u907f\u514d\u6e29\u5ea6\u53d8\u5316\u5f71\u54cd\u7ec6\u80de\u8d34\u58c1 &#8211; \u60ac\u6d6e\u5e73\u8861\uff1b\u56e0\u534a\u8d34\u58c1\u7279\u6027\uff0c\u57f9\u517b\u74f6\u9700\u5782\u76f4\u653e\u7f6e\uff0c\u907f\u514d\u503e\u659c\u5bfc\u81f4\u60ac\u6d6e\u7ec6\u80de\u805a\u96c6\u5728\u4e00\u4fa7\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u534a\u8d34\u58c1\u7279\u6027\uff09\uff1a\u2460\u7ec6\u80de\u6536\u96c6\uff1a\u540c\u65f6\u6536\u96c6\u8d34\u58c1\u6d88\u5316\u7ec6\u80de\u4e0e\u60ac\u6d6e\u7ec6\u80de\uff0c1000rpm \u79bb\u5fc3 8 \u5206\u949f\uff08\u957f\u4e8e JF-305 \u7684 5 \u5206\u949f\uff09\uff0c\u5f7b\u5e95\u53bb\u9664\u57f9\u517b\u57fa\u6b8b\u7559\uff1b\u2461\u51bb\u5b58\u6d53\u5ea6\uff1a\u7528\u9884\u51b7\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\u81f3 3\u00d710\u2076cells\/mL\uff08\u9ad8\u4e8e JF-305 \u7684 2.5\u00d710\u2076\uff09\uff0c\u8865\u507f\u590d\u82cf\u635f\u5931\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 60 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 4 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 2 \u5206\u949f\uff0c\u7acb\u5373\u7528\u542b\u53cc\u8840\u6e05\u7684 DMEM \u91cd\u60ac\uff0c\u63a5\u79cd\u540e 24 \u5c0f\u65f6\u5185\u4e0d\u6362\u6db2\uff0c\u5e2e\u52a9\u7ec6\u80de\u6062\u590d\u8d34\u58c1\u80fd\u529b\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u500d\u589e\u65f6\u95f4\uff1a25.7-40 \u5c0f\u65f6\uff08\u591a\u6587\u732e \/ \u673a\u6784\u9a8c\u8bc1\uff0c\u6570\u636e\u5dee\u5f02\u6e90\u4e8e\u57f9\u517b\u6761\u4ef6\uff09\uff1a\u2460\u6d53\u5ea6\u4f9d\u8d56\u7279\u6027\uff1a\u542b 10% FBS+2.5% \u9a6c\u8840\u6e05\u65f6 25.7-26 \u5c0f\u65f6\uff08\u6700\u5feb\uff09\uff0c\u4ec5\u542b 10% FBS \u65f6 30-40 \u5c0f\u65f6\uff0c\u65e0\u8840\u6e05\u65f6 18-40 \u5c0f\u65f6\uff08\u6ce2\u52a8\u5927\uff09\uff1b\u2461\u5b9e\u9a8c\u8bbe\u8ba1\uff1a\u9700\u6839\u636e\u5b9e\u9a8c\u76ee\u7684\u9009\u62e9\u8840\u6e05\u6d53\u5ea6\uff0c\u5982\u77ed\u671f\u836f\u7269\u7b5b\u9009\u7528\u6807\u51c6\u53cc\u8840\u6e05\uff0825.7 \u5c0f\u65f6\u500d\u589e\uff09\uff0c\u8840\u6e05\u5265\u593a\u5b9e\u9a8c\u7528\u65e0\u8840\u6e05\u57f9\u517b\u57fa\uff0818-40 \u5c0f\u65f6\uff09\uff1b\u4e0e JF-305 \u7684\u63a8\u6d4b\u500d\u589e\u76f8\u6bd4\uff0c\u6570\u636e\u66f4\u7cbe\u51c6\uff0c\u9002\u5408\u5f00\u5c55\u7cbe\u51c6\u65f6\u95f4\u4f9d\u8d56\u578b\u5b9e\u9a8c\uff08\u5982\u7ec6\u80de\u5468\u671f\u540c\u6b65\u5316\uff09\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57fa\u56e0\u8868\u8fbe\u60c5\u51b5\uff1ahuman colony stimulating factor, subclass I (CSF-I)\uff08\u96c6\u843d\u523a\u6fc0\u56e0\u5b50 I\uff09\u3001plasminogen activator\uff08\u7ea4\u6eb6\u9176\u539f\u6fc0\u6d3b\u5242\uff09\uff1a\u2460\u529f\u80fd\u5173\u8054\uff1aCSF-I \u53ef\u8c03\u63a7\u80bf\u7624\u76f8\u5173\u5de8\u566c\u7ec6\u80de\u6d78\u6da6\uff08\u8001\u5e74\u80f0\u817a\u764c\u5fae\u73af\u5883\u7279\u5f81\uff09\uff0c\u7ea4\u6eb6\u9176\u539f\u6fc0\u6d3b\u5242\u4fc3\u8fdb\u7ec6\u80de\u5916\u57fa\u8d28\u964d\u89e3\uff08\u4e0e\u534a\u8d34\u58c1\u7ec6\u80de\u7684\u5c40\u90e8\u4fb5\u88ad\u80fd\u529b\u76f8\u5173\uff09\uff1b\u2461\u5e94\u7528\u573a\u666f\uff1a\u901a\u8fc7 Western Blot \u68c0\u6d4b CSF-I \u8868\u8fbe\uff0c\u5206\u6790\u8001\u5e74\u80f0\u817a\u764c\u5fae\u73af\u5883\u8c03\u63a7\u673a\u5236\uff1b\u901a\u8fc7\u7ea4\u6eb6\u9176\u6d3b\u6027\u68c0\u6d4b\uff08\u5982\u53d1\u8272\u5e95\u7269\u6cd5\uff09\uff0c\u63a2\u7d22\u5176\u4e0e\u7ec6\u80de\u534a\u8d34\u58c1\u751f\u957f\u7684\u5173\u8054\uff0c\u540c\u65f6\u53ef\u4f5c\u4e3a\u7ec6\u80de\u8eab\u4efd\u9a8c\u8bc1\u7684\u8865\u5145\u6307\u6807\uff09<\/li>\n<\/ul>\n<ul>\n<li 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