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\u201c\u4e0a\u76ae\u5f62\u6001\u3001\u9ad8\u8840\u6e05\u4f9d\u8d56\u3001\u80f0\u5c9b\u7d20\u54cd\u5e94\u201d \u6838\u5fc3\u5c5e\u6027\uff0c\u5efa\u8bae\u4f20\u4ee3\u6b21\u6570\u4e25\u683c\u63a7\u5236\u5728 10 \u4ee3\u4ee5\u5185\uff0c\u9632\u6b62\u957f\u671f\u4f20\u4ee3\u5bfc\u81f4\u7279\u6027\u6f02\u79fb\uff0c\u5982\u4e0a\u76ae\u5f62\u6001\u5411\u68ad\u5f62\u8f6c\u53d8\u3001\u80f0\u5c9b\u7d20\u8c03\u63a7\u589e\u6b96\u80fd\u529b\u4e0b\u964d\uff08\u589e\u6b96\u901f\u5ea6\u53d8\u5316\uff1e20%\uff09\u3001\u80f0\u817a\u76f8\u5173\u6807\u5fd7\u7269\uff08\u5982 CK18\uff09\u8868\u8fbe\u964d\u4f4e\uff0c\u786e\u4fdd\u7ec6\u80de\u59cb\u7ec8\u8d34\u5408\u80f0\u817a\u764c\u4e0a\u76ae\u7ec6\u80de\u7684\u5b9e\u9a8c\u9700\u6c42\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">STR \u9274\u5b9a\uff1aSTR \u9274\u5b9a\u901a\u8fc7\uff08\u6838\u5fc3\u4fdd\u969c\uff1a\u2460\u901a\u8fc7\u81f3\u5c11 16 \u4e2a\u6838\u5fc3 STR \u4f4d\u70b9\u68c0\u6d4b\uff08\u5982 Amelogenin\u3001CSF1PO\u3001D13S317 \u7b49\uff09\uff0c\u7ed3\u679c\u4e0e\u80f0\u817a\u764c\u7ec6\u80de\u6807\u51c6\u56fe\u8c31\u4e00\u81f4\uff0c\u65e0 SW1990\u3001PANC-1 \u7684 STR \u4f4d\u70b9\u7279\u5f81\uff0c\u6392\u9664\u4ea4\u53c9\u6c61\u67d3\uff1b\u2461\u53ef\u901a\u8fc7\u5bf9\u6bd4\u56fd\u5185\u80f0\u817a\u764c\u7ec6\u80de\u5e93\uff08\u5982\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u57fa\u7840\u533b\u5b66\u7814\u7a76\u6240\u7ec6\u80de\u5e93\uff09\u7684\u901a\u7528\u80f0\u817a\u764c\u7ec6\u80de STR \u6570\u636e\uff0c\u8f85\u52a9\u786e\u8ba4\u7ec6\u80de\u8eab\u4efd\uff0c\u540c\u65f6\u4e0e SW1990 \u7684 \u201c\u813e\u8f6c\u79fb STR\u201d\u3001PANC-1 \u7684 \u201c\u80f0\u7ba1\u539f\u53d1 STR\u201d \u5bf9\u6bd4\uff0c\u660e\u786e PANC 05.04 \u7684\u72ec\u7279\u4f4d\u70b9\uff0c\u907f\u514d\u4e0e\u4e9a\u578b\u7279\u5f02\u6216\u8f6c\u79fb\u578b\u7ec6\u80de\u7cfb\u6df7\u6dc6\uff0c\u786e\u4fdd\u5b9e\u9a8c\u7528\u7ec6\u80de\u8eab\u4efd\u51c6\u786e\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u751f\u7269\u5b89\u5168\u7b49\u7ea7\uff1a1\uff08\u4e0e SW1990\u3001PANC-1 \u4e00\u81f4\uff0c\u4f4e\u4e8e BEAS-2B \u7684 2 \u7ea7\uff1b\u64cd\u4f5c\u65f6\u9700\u9075\u5faa\u751f\u7269\u5b89\u5168\u4e00\u7ea7\u5b9e\u9a8c\u5ba4\u9632\u62a4\u89c4\u8303\uff0c\u5b9e\u9a8c\u5e9f\u5f03\u7269\u9700\u7ecf 121\u2103\u9ad8\u538b\u706d\u83cc 30 \u5206\u949f\u5904\u7406\uff0c\u907f\u514d\u7ec6\u80de\u6b8b\u7559\u6c61\u67d3\uff1b\u56e0\u57f9\u517b\u57fa\u542b\u91cd\u7ec4\u80f0\u5c9b\u7d20\uff0c\u9700\u6ce8\u610f\u8bd5\u5242\u50a8\u5b58\uff08-20\u2103\u5206\u88c5\u4fdd\u5b58\uff09\uff0c\u907f\u514d\u53cd\u590d\u51bb\u878d\u5f71\u54cd\u6d3b\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u89c4\u683c\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216\u8005 1mL \u51bb\u5b58\u7ba1\u5305\u88c5\uff08\u8ba1\u6570\u65f6\u56e0\u4e0a\u76ae\u7ec6\u80de\u6392\u5217\u89c4\u6574\uff0c\u8f7b\u67d4\u5439\u6253\u5373\u53ef\u83b7\u5f97\u5355\u7ec6\u80de\u60ac\u6db2\uff0c\u8ba1\u6570\u51c6\u786e\u6027\u9ad8\uff08\u8bef\u5dee\uff1c5%\uff09\uff0c\u65e0 SW1990 \u7684\u4f4e\u690d\u677f\u7387\u8ba1\u6570\u5e72\u6270\u3001PANC-1 \u7684\u805a\u56e2\u8ba1\u6570\u504f\u5dee\uff1b\u51bb\u5b58\u7ba1\u542b 10% \u4e8c\u7532\u57fa\u4e9a\u781c\uff08DMSO\uff09\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u590d\u82cf\u540e 48 \u5c0f\u65f6\u5b58\u6d3b\u7387\u226585%\uff08\u4e0e PANC-1 \u4e00\u81f4\uff0c\u9ad8\u4e8e SW1990 \u7684 80%\uff09\uff0c\u4e14\u4e0a\u76ae\u6837\u5f62\u6001\u3001\u80f0\u5c9b\u7d20\u54cd\u5e94\u7279\u6027\u65e0\u663e\u8457\u6539\u53d8\uff1bT25 \u74f6\u53d1\u8d27\u65f6\u7ec6\u80de\u5bc6\u5ea6\u8fbe 70%-75%\uff0c\u6807\u6ce8 \u201c\u4eba\u80f0\u817a\u764c\u4e0a\u76ae\u7ec6\u80de\uff0c1640+15% FBS + \u91cd\u7ec4\u80f0\u5c9b\u7d20\uff0c\u5efa\u8bae\u68c0\u6d4b\u4e0a\u76ae\u6807\u5fd7\u7269\u201d\uff0c\u63d0\u9192\u7528\u6237\u5173\u6ce8\u9ad8\u8840\u6e05 + \u80f0\u5c9b\u7d20\u914d\u65b9\u53ca\u4e0a\u76ae\u5c5e\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u652f\u539f\u4f53\u68c0\u6d4b\uff1a\u65e0\uff08\u6bcf\u6279\u6b21\u7ec6\u80de\u901a\u8fc7 PCR \u6cd5\u68c0\u6d4b\u652f\u539f\u4f53\uff08\u8986\u76d6\u80ba\u708e\u652f\u539f\u4f53\u3001\u4eba\u578b\u652f\u539f\u4f53\u3001\u89e3\u8132\u652f\u539f\u4f53\uff09\uff0c\u7ed3\u679c\u4e3a\u9634\u6027\uff0c\u907f\u514d\u652f\u539f\u4f53\u6c61\u67d3\u5f71\u54cd\u9ad8\u8840\u6e05\u57f9\u517b\u4e0b\u7684\u589e\u6b96\u53ca\u80f0\u5c9b\u7d20\u8c03\u63a7\u5b9e\u9a8c\uff0c\u4fdd\u969c\u4e0e SW1990\u3001PANC-1 \u5e73\u884c\u5b9e\u9a8c\u7684\u53ef\u9760\u6027\uff0c\u5c24\u5176\u9002\u5408\u591a\u7ec6\u80de\u7cfb\u540c\u6b65\u5f00\u5c55\u7684\u5bf9\u6bd4\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4fdd\u85cf\u673a\u6784\uff1a\u672a\u660e\u786e\u6807\u6ce8\uff08\u5efa\u8bae\u5b9e\u9a8c\u524d\u54a8\u8be2\u4f9b\u5e94\u5546\u83b7\u53d6\u4fdd\u85cf\u673a\u6784\u4fe1\u606f\uff08\u5982 ATCC\u3001DSMZ \u6216\u56fd\u5185\u80f0\u817a\u764c\u7ec6\u80de\u5e93\uff09\uff0c\u901a\u8fc7\u4fdd\u85cf\u673a\u6784\u6570\u636e\u5e93\u67e5\u8be2\u7ec6\u80de\u8be6\u7ec6\u80cc\u666f\uff08\u5982\u6027\u522b\u5e74\u9f84\u3001\u75c5\u7406\u4e9a\u578b\u3001\u662f\u5426\u542b\u80f0\u817a\u764c\u5178\u578b\u7a81\u53d8\uff08KRAS\u3001p53\uff09\uff09\uff0c\u8865\u5145\u5b8c\u5584\u7ec6\u80de\u57fa\u7840\u4fe1\u606f\uff0c\u63d0\u5347\u5b9e\u9a8c\u53ef\u8ffd\u6eaf\u6027\u4e0e\u7ed3\u679c\u91cd\u590d\u6027\uff0c\u4e0e SW1990 \u7684\u672a\u660e\u786e\u4fdd\u85cf\u3001PANC-1 \u7684 ATCC \u4fdd\u85cf\u5f62\u6210\u4e92\u8865\uff0c\u4fbf\u4e8e\u56fd\u5185\u5916\u79d1\u7814\u534f\u4f5c\u53ca\u6587\u732e\u7ed3\u679c\u590d\u73b0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u57fa\uff1a1640\uff08RPMI-1640\uff09+15% FBS+PS\uff08\u9752\u9709\u7d20 &#8211; \u94fe\u9709\u7d20\uff09+0.01mg\/ml rh insulin\uff08\u91cd\u7ec4\u80f0\u5c9b\u7d20\uff09\uff08\u6838\u5fc3\u7279\u8272\uff0c\u4e0e SW1990\u3001PANC-1 \u5bf9\u6bd4\uff09\uff1a\u2460\u9ad8\u8840\u6e05 + \u80f0\u5c9b\u7d20\u914d\u65b9\uff1a\u91c7\u7528 RPMI-1640 \u57f9\u517b\u57fa\uff0c\u533a\u522b\u4e8e SW1990 \u7684 L-15\u3001PANC-1 \u7684 DMEM\uff1b15% FBS\uff08\u9ad8\u4e8e\u5e38\u89c4 10%\uff09\u63d0\u4f9b\u5145\u8db3\u8425\u517b\u652f\u6301\u4e0a\u76ae\u7ec6\u80de\u589e\u6b96\uff0c0.01mg\/ml \u91cd\u7ec4\u80f0\u5c9b\u7d20\u6fc0\u6d3b\u80f0\u5c9b\u7d20\u4fe1\u53f7\u901a\u8def\uff08\u5982 PI3K-AKT\uff09\uff0c\u8c03\u63a7\u80f0\u817a\u764c\u7ec6\u80de\u4ee3\u8c22\u53ca\u751f\u957f\uff0c\u9002\u914d\u4e0a\u76ae\u7ec6\u80de\u80fd\u91cf\u9700\u6c42\uff1b\u2461\u6210\u5206\u8981\u6c42\uff1aFBS \u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u3001\u6279\u6b21\u95f4\u5dee\u5f02\u5c0f\u7684\u4f18\u8d28\u80ce\u725b\u8840\u6e05\uff08\u5efa\u8bae\u9009\u7528\u5357\u7f8e\u6765\u6e90\uff09\uff0c\u91cd\u7ec4\u80f0\u5c9b\u7d20\u9700\u7528\u65e0\u8840\u6e05 1640 \u57f9\u517b\u57fa\u7a00\u91ca\u81f3\u5de5\u4f5c\u6d53\u5ea6\uff0c\u73b0\u914d\u73b0\u7528\uff1bPS \u7ec8\u6d53\u5ea6 100U\/mL \u9752\u9709\u7d20\u3001100\u03bcg\/mL \u94fe\u9709\u7d20\uff1b\u2462\u914d\u5236\u4e0e\u50a8\u5b58\uff1a\u57f9\u517b\u57fa\u9700\u5728 2-8\u2103\u907f\u5149\u4fdd\u5b58\uff0c\u5f00\u5c01\u540e 1 \u5468\u5185\u4f7f\u7528\u5b8c\u6bd5\uff08\u80f0\u5c9b\u7d20\u6613\u964d\u89e3\uff09\uff0c\u65e0\u9700\u7279\u6b8a\u6c14\u76f8\u5e73\u8861\uff08\u5e38\u89c4 CO\u2082\u73af\u5883\u5373\u53ef\uff09\uff0c\u64cd\u4f5c\u4fbf\u5229\u6027\u9ad8\uff0c\u4ec5\u9700\u6ce8\u610f\u80f0\u5c9b\u7d20\u7684\u6dfb\u52a0\u65f6\u673a\uff08\u57f9\u517b\u57fa\u9884\u70ed\u540e\u518d\u52a0\u5165\uff0c\u907f\u514d\u53d8\u6027\uff09\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u57f9\u517b\u6761\u4ef6\uff1a\u6c14\u76f8\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff1b\u6e29\u5ea6\uff1a37\u2103\uff08\u4e0e PANC-1 \u4e00\u81f4\uff0c\u4e0e SW1990 \u7684 100% \u7a7a\u6c14\u5dee\u5f02\u663e\u8457\uff09\uff1a\u2460\u73af\u5883\u63a7\u5236\uff1a\u6e7f\u5ea6\u7ef4\u6301\u5728 70%-80%\uff08\u5e38\u89c4\u6807\u51c6\uff09\uff0cCO\u2082\u6d53\u5ea6\u7a33\u5b9a\u5728 5%\uff0c\u65e0\u9700\u50cf SW1990 \u90a3\u6837\u7279\u6b8a\u63a7\u5236\u6c14\u76f8\uff1b\u57f9\u517b\u7bb1\u63a7\u6e29\u6ce2\u52a8\u2264\u00b10.5\u2103\uff0c\u907f\u514d\u6e29\u5ea6\u53d8\u5316\u5f71\u54cd\u80f0\u5c9b\u7d20\u6d3b\u6027\u53ca\u7ec6\u80de\u589e\u6b96\uff1b\u2461\u73af\u5883\u517c\u5bb9\u6027\uff1a\u53ef\u4e0e PANC-1\u3001PATU 8988t \u7b49 CO\u2082\u4f9d\u8d56\u578b\u7ec6\u80de\u5171\u540c\u653e\u7f6e\uff0c\u65e0\u9700\u5355\u72ec\u9694\u79bb\uff0c\u4fbf\u4e8e\u591a\u7ec6\u80de\u682a\u5e76\u884c\u5f00\u5c55 \u201c\u80f0\u817a\u764c\u7ec6\u80de\u591a\u7c7b\u578b\u201d \u5bf9\u6bd4\u5b9e\u9a8c\uff1b\u2462pH \u76d1\u6d4b\uff1aRPMI-1640 \u57f9\u517b\u57fa pH \u7a33\u5b9a\u8303\u56f4 7.2-7.4\uff0c\u82e5\u53d1\u73b0\u989c\u8272\u5f02\u5e38\uff08\u53d8\u9ec4 \/ \u53d8\u7d2b\uff09\uff0c\u9700\u53ca\u65f6\u66f4\u6362\u65b0\u9c9c\u57f9\u517b\u57fa\uff0c\u540c\u65f6\u68c0\u67e5 CO\u2082\u4f9b\u5e94\u662f\u5426\u6b63\u5e38\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u51bb\u5b58\u6761\u4ef6\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff08\u51bb\u5b58\u64cd\u4f5c\u9002\u914d\u9ad8\u8840\u6e05 + \u80f0\u5c9b\u7d20\u7279\u6027\uff09\uff1a\u2460\u7ec6\u80de\u6536\u96c6\uff1a\u9009\u62e9\u5bf9\u6570\u751f\u957f\u671f\u4e2d\u671f\u7ec6\u80de\uff08\u5bc6\u5ea6 75%\uff0c\u80f0\u5c9b\u7d20\u54cd\u5e94\u6700\u4f73\uff09\uff0c\u7528 0.25% \u80f0\u9176 &#8211; EDTA \u5b75\u80b2 2-3 \u5206\u949f\uff0c\u955c\u4e0b\u89c1\u7ec6\u80de\u95f4\u9699\u589e\u5927\u5373\u53ef\u7ec8\u6b62\uff0c\u8f7b\u67d4\u5439\u6253\u907f\u514d\u7ec6\u80de\u7834\u788e\uff1b\u2461\u51bb\u5b58\u6d53\u5ea6\uff1a1000rpm \u79bb\u5fc3 5 \u5206\u949f\u6536\u96c6\u7ec6\u80de\uff0c\u7528\u9884\u51b7\u7684\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u91cd\u60ac\uff0c\u8c03\u6574\u6d53\u5ea6\u81f3 2.5\u00d710\u2076cells\/mL\uff08\u4e0e PANC-1\u3001SW1990 \u4e00\u81f4\uff09\uff1b\u2462\u51bb\u5b58\u6d41\u7a0b\uff1a4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103\u653e\u7f6e 2 \u5c0f\u65f6\uff08\u7a0b\u5e8f\u964d\u6e29\u76d2\uff09\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u4fdd\u5b58\uff1b\u590d\u82cf\u65f6 37\u2103\u6c34\u6d74 1 \u5206\u949f\u878d\u5316\uff0c\u79bb\u5fc3\u540e\u7528\u9884\u70ed\u7684\u542b\u80f0\u5c9b\u7d20\u5b8c\u6574\u57f9\u517b\u57fa\u91cd\u60ac\u76f4\u63a5\u63a5\u79cd\uff0c\u907f\u514d DMSO \u5f71\u54cd\u80f0\u5c9b\u7d20\u53d7\u4f53\uff08\u5982 IR\u03b2\uff09\u6d3b\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u7ec6\u80de\u8d27\u671f\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u5e93\u5b58\u7ec6\u80de\u5747\u7ecf\u8fc7 RPMI-1640+15% FBS + \u80f0\u5c9b\u7d20\u9002\u5e94\u6027\u57f9\u517b\u3001\u652f\u539f\u4f53\u68c0\u6d4b\u53ca\u7ec6\u80de\u5f62\u6001\u9a8c\u8bc1\uff08\u4e0a\u76ae\u6837\uff09\uff0c\u6838\u5fc3\u57f9\u517b\u7279\u6027\u7a33\u5b9a\uff0c\u4e0e SW1990\u3001PANC-1 \u7684 1 \u5468\u8d27\u671f\u4e00\u81f4\uff1b\u56e0\u9700\u6dfb\u52a0\u91cd\u7ec4\u80f0\u5c9b\u7d20\uff0c\u4e0b\u5355\u540e\u9700\u63d0\u524d 1 \u5929\u51c6\u5907\u57f9\u517b\u57fa\uff0c\u53ef\u4e0e SW1990\u3001PANC-1 \u540c\u6b65\u4e0b\u5355\uff0c\u901a\u8fc7\u8c03\u6574 SW1990 \u7684\u63a5\u79cd\u65f6\u95f4\uff08\u65e9 2 \u5929\uff09\uff0c\u4fdd\u969c \u201c\u80f0\u817a\u764c\u7ec6\u80de\u591a\u7c7b\u578b\u201d \u5e73\u884c\u5b9e\u9a8c\u7684\u65f6\u95f4\u4e00\u81f4\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u8fd0\u8f93\u65b9\u5f0f\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u91c7\u7528\u9632\u9707\u6052\u6e29\u6ce1\u6cab\u7bb1\uff0c\u5185\u7f6e 2-8\u2103\u51b0\u888b\u53ca\u6e29\u5ea6\u76d1\u6d4b\u5361\uff0c\u9644\u5e26\u5355\u72ec\u7684\u91cd\u7ec4\u80f0\u5c9b\u7d20\u8bd5\u5242\uff08-20\u2103\u51b0\u888b\u51b7\u85cf\uff09\uff1b\u8fd0\u8f93\u8fc7\u7a0b\u4e2d\u7528\u56fa\u5b9a\u652f\u67b6\u9632\u6b62\u57f9\u517b\u74f6\u6643\u52a8\uff0c\u51cf\u5c11\u4e0a\u76ae\u6837\u8d34\u58c1\u7ec6\u80de\u8131\u843d\uff1b\u5230\u8fbe\u540e\u7acb\u5373\u5c06\u80f0\u5c9b\u7d20\u52a0\u5165\u57fa\u7840\u57f9\u517b\u57fa\u914d\u5236\u5b8c\u6574\u4f53\u7cfb\uff0c\u653e\u5165 5% CO\u2082\u57f9\u517b\u7bb1\uff0c24 \u5c0f\u65f6\u540e\u89c2\u5bdf\u8d34\u58c1\u72b6\u6001\uff0c48 \u5c0f\u65f6\u5185\u9996\u6b21\u6362\u6db2\uff0c\u65e0\u9700\u50cf SW1990 \u90a3\u6837\u7279\u6b8a\u6362\u6c14\uff09\/ \u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff0c\u5e72\u51b0\u7528\u91cf\u6309\u8fd0\u8f93\u65f6\u957f\u8ba1\u7b97\uff0c\u56fd\u5185\u8fd0\u8f93\u914d\u5907 1kg \u5e72\u51b0\u5373\u53ef\uff0c\u786e\u4fdd\u5230\u8fbe\u65f6\u5e72\u51b0\u5269\u4f59\u91cf\u226560%\uff0c\u7ef4\u6301\u7ec6\u80de\u51bb\u5b58\u72b6\u6001\uff09 \u987a\u4e30\u5feb\u9012\uff08\u4f18\u5148\u9009\u62e9\u6b21\u6668\u8fbe\u670d\u52a1\uff0c\u6700\u5927\u9650\u5ea6\u7f29\u77ed\u8fd0\u8f93\u65f6\u95f4\uff0c\u964d\u4f4e\u7ec6\u80de\u6d3b\u6027\u635f\u5931\u53ca\u80f0\u5c9b\u7d20\u53d7\u4f53\u6d3b\u6027\u6ce2\u52a8\u98ce\u9669\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\">\u4f9b\u5e94\u9650\u5236\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e SW1990\u3001PANC-1 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data-list=\"bullet1\">\u7279\u522b\u8bf4\u660e\uff1a\u4ee5\u4e0b\u7ec6\u80de\u57f9\u517b\u51bb\u5b58\u5904\u7406\u4ec5\u4f9b\u53c2\u8003\uff0c\u5177\u4f53\u64cd\u4f5c\u6b65\u9aa4\u4ee5\u968f\u8d27\u4ea7\u54c1\u8bf4\u660e\u4e66\u4e3a\u4e3b\uff1b\u8be5\u7ec6\u80de\u5b9e\u9a8c\u64cd\u4f5c\u9700\u91cd\u70b9\u5173\u6ce8\uff1a\u2460\u80f0\u5c9b\u7d20\u529f\u80fd\u9a8c\u8bc1\uff1a\u9996\u6b21\u57f9\u517b\u65f6\uff0c\u5efa\u8bae\u901a\u8fc7\u4ee5\u4e0b\u5b9e\u9a8c\u786e\u8ba4\u80f0\u5c9b\u7d20\u54cd\u5e94\u7279\u6027\uff1aa. \u8bbe\u7f6e\u80f0\u5c9b\u7d20\u6d53\u5ea6\u68af\u5ea6\uff080\u30010.005\u30010.01\u30010.02mg\/ml\uff09\uff0c\u57f9\u517b 48 \u5c0f\u65f6\u540e\u7528 CCK-8 \u68c0\u6d4b\u589e\u6b96\u7387\uff0c\u9a8c\u8bc1 0.01mg\/ml \u4e3a\u6700\u4f73\u6d53\u5ea6\uff1bb. \u901a\u8fc7 Western Blot 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30% \u4ee5\u4e0a\uff09\uff0c\u4e0d\u53ef\u7701\u7565\u91cd\u7ec4\u80f0\u5c9b\u7d20\uff08\u7f3a\u5931\u540e\u7ec6\u80de\u589e\u6b96\u4e0b\u964d 40%\uff09\uff0c\u66f4\u6362 FBS \u6279\u6b21\u65f6\u9700\u540c\u6b65\u68c0\u6d4b\u80f0\u5c9b\u7d20\u9002\u914d\u6027\uff0c\u786e\u4fdd\u57f9\u517b\u4f53\u7cfb\u7a33\u5b9a\u3002<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"21\" data-line=\"true\"><\/div>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09 \u7ec6\u80de\u540d\u79f0\uff1aPANC 05.04 \u4eba\u80f0\u817a\u764c\u7ec6\u80de\uff08\u4e0e SW<\/p>\n<div class=\"more-link\">\n             <a href=\"https:\/\/www.fengyaosw.com\/index.php\/product\/panc05-04%e4%ba%ba%e8%83%b0%e8%85%ba%e7%99%8c%e7%bb%86%e8%83%9e-%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/\" class=\"theme-btn\"><span>\u7ee7\u7eed\u9605\u8bfb <\/span><i class=\"bi bi-arrow-right\"><\/i><\/a>\n        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