{"id":2565,"date":"2025-10-08T14:15:09","date_gmt":"2025-10-08T06:15:09","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=2565"},"modified":"2025-10-08T14:15:32","modified_gmt":"2025-10-08T06:15:32","slug":"%e7%bb%86%e8%83%9e%e6%b0%b8%e7%94%9f%e5%8c%96%e6%9c%8d%e5%8a%a1","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/%e7%bb%86%e8%83%9e%e6%b0%b8%e7%94%9f%e5%8c%96%e6%9c%8d%e5%8a%a1\/","title":{"rendered":"\u7ec6\u80de\u6c38\u751f\u5316\u670d\u52a1"},"content":{"rendered":"<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u6280\u672f\u6838\u5fc3\u6982\u8ff0<\/h2>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u6c38\u751f\u5316\u6280\u672f\u901a\u8fc7\u57fa\u56e0\u8f6c\u67d3\uff08\u5982\u6162\u75c5\u6bd2\u4ecb\u5bfc\uff09\u5c06\u5916\u6e90\u6027\u6c38\u751f\u5316\u57fa\u56e0\uff08\u5982 SV40 T \u6297\u539f\u3001hTERT\uff09\u5bfc\u5165\u539f\u4ee3\u7ec6\u80de\uff0c\u6216\u8bf1\u5bfc\u8870\u8001\u76f8\u5173\u57fa\u56e0\u7a81\u53d8\uff0c\u5e2e\u52a9\u7ec6\u80de\u7a81\u7834 \u201c\u6d77\u5f17\u5229\u514b\u6781\u9650\u201d\uff0c\u83b7\u5f97\u65e0\u9650\u589e\u6b96\u80fd\u529b\u3002\u8be5\u6280\u672f\u53ef\u5efa\u7acb\u7a33\u5b9a\u5747\u4e00\u7684\u6c38\u751f\u5316\u7ec6\u80de\u682a\uff0c\u65e2\u4fdd\u7559\u4eb2\u672c\u7ec6\u80de\u7684\u57fa\u56e0\u578b\u4e0e\u8868\u578b\uff0c\u53c8\u89e3\u51b3\u539f\u4ee3\u7ec6\u80de\u4f20\u4ee3\u56f0\u96be\u3001\u6613\u8870\u8001\u7684\u95ee\u9898\uff0c\u4e3a\u7ec6\u80de\u751f\u7269\u5b66\u7814\u7a76\u3001\u80bf\u7624\u673a\u5236\u63a2\u7d22\u53ca\u836f\u7269\u7814\u53d1\u63d0\u4f9b\u7406\u60f3\u4f53\u5916\u6a21\u578b\u3002<\/div>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e8c\u3001\u6838\u5fc3\u6280\u672f\u539f\u7406\u4e0e\u65b9\u6cd5<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u4e3b\u6d41\u6c38\u751f\u5316\u65b9\u6cd5\u5bf9\u6bd4<\/h3>\n<div class=\"auto-hide-last-sibling-br mdbox-table-root table-container-cYf_5N\" data-scroll-inline-overflow=\"true\" data-scroll-inline-at-start=\"true\" data-scroll-inline-start-overflow=\"false\" data-scroll-inline-at-end=\"false\" data-scroll-inline-end-overflow=\"true\">\n<div class=\"table-scroll-container-Gyf4hQ mdbox-table-scroll-container\">\n<table>\n<thead>\n<tr>\n<th>\u65b9\u6cd5\u5206\u7c7b<\/th>\n<th>\u6838\u5fc3\u673a\u5236<\/th>\n<th>\u9002\u7528\u7ec6\u80de<\/th>\n<th>\u4f18\u52bf<\/th>\n<th>\u6ce8\u610f\u4e8b\u9879<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td><strong>\u75c5\u6bd2\u57fa\u56e0\u8f6c\u67d3<\/strong><\/td>\n<td>\u75c5\u6bd2\u57fa\u56e0\uff08\u5982 SV40 T \u6297\u539f\u3001EBV\u3001HPV E6\/E7\uff09\u6574\u5408\u5165\u7ec6\u80de\uff0c\u6539\u53d8\u589e\u6b96\u8c03\u63a7\uff0c\u7ed5\u8fc7\u5468\u671f\u68c0\u67e5\u70b9<\/td>\n<td>&#8211; SV40\uff1a\u591a\u79cd\u4e0a\u76ae\u7ec6\u80de\u3001\u6210\u7ea4\u7ef4\u7ec6\u80de<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>&#8211; EBV\uff1aB \u6dcb\u5df4\u7ec6\u80de<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>&#8211; HPV\uff1a\u4e0a\u76ae\u7ec6\u80de\uff08\u5305\u76ae\u3001\u4e73\u817a\u3001\u53e3\u8154\u7b49\uff09<\/td>\n<td>\u64cd\u4f5c\u6210\u719f\u3001\u6c38\u751f\u5316\u6548\u7387\u9ad8<\/td>\n<td>\u53ef\u80fd\u5bfc\u81f4\u7ec6\u80de\u8868\u578b\u8f7b\u5fae\u6539\u53d8\uff0c\u90e8\u5206\u75c5\u6bd2\uff08\u5982 SV40\uff09\u53ef\u80fd\u4f7f\u7ec6\u80de\u5177\u5907\u80bf\u7624\u8f6c\u5316\u503e\u5411<\/td>\n<\/tr>\n<tr>\n<td><strong>\u7aef\u7c92\u9176\u6fc0\u6d3b<\/strong><\/td>\n<td>\u5bfc\u5165\u4eba\u7aef\u7c92\u9176\u50ac\u5316\u4e9a\u57fa\uff08hTERT\uff09\u57fa\u56e0\uff0c\u7a33\u5b9a\u7aef\u7c92\u957f\u5ea6\uff0c\u907f\u514d\u7ec6\u80de\u56e0\u7aef\u7c92\u8017\u5c3d\u51cb\u4ea1<\/td>\n<td>\u591a\u6570\u4eba\u6e90 \/ \u52a8\u7269\u6e90\u4f53\u7ec6\u80de\uff08\u5982\u5e72\u7ec6\u80de\u3001\u514d\u75ab\u7ec6\u80de\u3001\u4e0a\u76ae\u7ec6\u80de\uff09<\/td>\n<td>\u7ec6\u80de\u8868\u578b\u66f4\u63a5\u8fd1\u539f\u4ee3\u7ec6\u80de\uff0c\u5b89\u5168\u6027\u9ad8<\/td>\n<td>\u5355\u72ec\u4f7f\u7528\u5bf9\u90e8\u5206\u7ec6\u80de\uff08\u5982\u9ad8\u5ea6\u5206\u5316\u7ec6\u80de\uff09\u6548\u679c\u6709\u9650\uff0c\u9700\u7ed3\u5408\u764c\u57fa\u56e0\u8f85\u52a9<\/td>\n<\/tr>\n<tr>\n<td><strong>\u764c\u57fa\u56e0 \/ \u6291\u764c\u57fa\u56e0\u8c03\u63a7<\/strong><\/td>\n<td>\u8f6c\u67d3\u539f\u764c\u57fa\u56e0\uff08\u5982 c-Myc\u3001c-Jun\uff09\u6fc0\u6d3b\u589e\u6b96\u4fe1\u53f7\uff0c\u6216\u6572\u9664\u6291\u764c\u57fa\u56e0\uff08\u5982 p53\u3001pRb\uff09\u89e3\u9664\u8870\u8001\u6291\u5236<\/td>\n<td>\u96be\u6c38\u751f\u5316\u7684\u7279\u6b8a\u7ec6\u80de\uff08\u5982\u90e8\u5206\u795e\u7ecf\u7ec6\u80de\u3001\u80f0\u5c9b\u7ec6\u80de\uff09<\/td>\n<td>\u53ef\u9488\u5bf9\u6027\u89e3\u51b3\u7279\u5b9a\u7ec6\u80de\u6c38\u751f\u5316\u96be\u9898<\/td>\n<td>\u9700\u7cbe\u51c6\u8c03\u63a7\u57fa\u56e0\u8868\u8fbe\uff0c\u907f\u514d\u8fc7\u5ea6\u6fc0\u6d3b\u5bfc\u81f4\u7ec6\u80de\u6076\u6027\u8f6c\u5316<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u5173\u952e\u6280\u672f\u4f18\u52bf<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u7a81\u7834\u589e\u6b96\u9650\u5236\uff1a\u89e3\u51b3\u539f\u4ee3\u7ec6\u80de\u4f20\u4ee3\u6b21\u6570\u5c11\uff08\u901a\u5e38 5-10 \u4ee3\uff09\u3001\u6613\u8870\u8001\u7684\u95ee\u9898\uff0c\u6c38\u751f\u5316\u7ec6\u80de\u53ef\u7a33\u5b9a\u4f20\u4ee3 50 \u4ee3\u4ee5\u4e0a\u3002<\/li>\n<li>\u4fdd\u7559\u7ec6\u80de\u7279\u6027\uff1a\u6c38\u751f\u5316\u7ec6\u80de\u57fa\u56e0\u578b\u3001\u8868\u578b\u4e0e\u4eb2\u672c\u7ec6\u80de\u9ad8\u5ea6\u4e00\u81f4\uff0c\u751f\u7406\u529f\u80fd\uff08\u5982\u5206\u6ccc\u3001\u5206\u5316\u6f5c\u80fd\uff09\u57fa\u672c\u4e0d\u53d8\u3002<\/li>\n<li>\u964d\u4f4e\u5b9e\u9a8c\u6210\u672c\uff1a\u51cf\u5c11\u539f\u4ee3\u7ec6\u80de\u53cd\u590d\u5206\u79bb\u3001\u8d2d\u4e70\u7684\u9891\u7387\uff0c\u8282\u7701\u65f6\u95f4\u4e0e\u7ecf\u8d39\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e09\u3001\u670d\u52a1\u5185\u5bb9\u4e0e\u6d41\u7a0b<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u6838\u5fc3\u670d\u52a1\u5185\u5bb9<\/h3>\n<ol class=\"auto-hide-last-sibling-br\">\n<li><strong>\u524d\u671f\u51c6\u5907<\/strong>\uff1a\u539f\u4ee3\u7ec6\u80de\u5206\u79bb\u7eaf\u5316\uff08\u5ba2\u6237\u63d0\u4f9b\u7ec6\u80de\u6750\u6599\uff0c\u6216\u4ece\u516c\u53f8\u539f\u4ee3\u7ec6\u80de\u5e93\u9009\u62e9\uff09\u3002<\/li>\n<li><strong>\u8f7d\u4f53\u6784\u5efa\u4e0e\u611f\u67d3<\/strong>\uff1a\u5236\u5907\u542b\u6c38\u751f\u5316\u57fa\u56e0\uff08\u5982 SV40 T \u6297\u539f\u3001hTERT\uff09\u7684\u6162\u75c5\u6bd2\uff0c\u611f\u67d3\u539f\u4ee3\u7ec6\u80de\u5e76\u57f9\u517b\u3002<\/li>\n<li><strong>\u7b5b\u9009\u4e0e\u9274\u5b9a<\/strong>\uff1a\u901a\u8fc7\u6297\u751f\u7d20\u7b5b\u9009\u9633\u6027\u7ec6\u80de\u682a\uff0c\u68c0\u6d4b\u7aef\u7c92\u9176\u57fa\u56e0\u8868\u8fbe\uff08\u5982 hTERT\uff09\u3001\u8870\u8001\u6807\u5fd7\u7269\uff08\u5982 \u03b2- \u534a\u4e73\u7cd6\u82f7\u9176\uff09\uff0c\u786e\u8ba4\u6c38\u751f\u5316\u6548\u679c\u3002<\/li>\n<li><strong>\u989d\u5916\u9a8c\u8bc1\uff08\u53ef\u9009\uff09<\/strong>\uff1a\u6839\u636e\u5ba2\u6237\u9700\u6c42\u8fdb\u884c\u8868\u578b\u9a8c\u8bc1\uff08mRNA \/ \u86cb\u767d\u8868\u8fbe\u68c0\u6d4b\uff09\u3001\u529f\u80fd\u9a8c\u8bc1\uff08\u5982\u7ec6\u80de\u589e\u6b96\u66f2\u7ebf\u3001\u5206\u5316\u80fd\u529b\u68c0\u6d4b\uff09\u3002<\/li>\n<\/ol>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u670d\u52a1\u6d41\u7a0b\u4e0e\u5468\u671f<\/h3>\n<ol class=\"auto-hide-last-sibling-br\">\n<li><strong>\u9700\u6c42\u786e\u8ba4<\/strong>\uff1a\u5ba2\u6237\u63d0\u4f9b\u539f\u4ee3\u7ec6\u80de\uff08\u6216\u9009\u62e9\u7ec6\u80de\u7c7b\u578b\uff09\uff0c\u660e\u786e\u6c38\u751f\u5316\u65b9\u6cd5\uff08\u5982 hTERT \u4ecb\u5bfc\u3001SV40 \u4ecb\u5bfc\uff09\u4e0e\u9a8c\u8bc1\u9700\u6c42\u3002<\/li>\n<li><strong>\u5b9e\u9a8c\u6267\u884c<\/strong>\uff1a\u6162\u75c5\u6bd2\u5236\u5907\u2192\u7ec6\u80de\u611f\u67d3\u2192\u7b5b\u9009\u7eaf\u5316\u2192\u9274\u5b9a\u68c0\u6d4b\uff084-8 \u5468\uff0c\u5177\u4f53\u89c6\u7ec6\u80de\u7c7b\u578b\u8c03\u6574\uff09\u3002<\/li>\n<li><strong>\u7ed3\u679c\u4ea4\u4ed8<\/strong>\uff1a\u6c38\u751f\u5316\u7ec6\u80de\u682a\uff081\u00d710\u2076cells\/T25 \u74f6\u6216\u51bb\u5b58\u7ba1\uff09\u3001\u5b8c\u6574\u5b9e\u9a8c\u62a5\u544a\uff08\u542b\u65b9\u6cd5\u3001\u7b5b\u9009\u7ed3\u679c\u3001\u9274\u5b9a\u6570\u636e\uff09\u3002<\/li>\n<\/ol>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">3. \u6536\u8d39\u6807\u51c6<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u57fa\u7840\u670d\u52a1\uff1a15000 \u5143 \/ \u682a\uff08\u542b\u7ec6\u80de\u6c38\u751f\u5316\u6784\u5efa\u3001\u57fa\u7840\u9274\u5b9a\uff1a\u7aef\u7c92\u9176\u8868\u8fbe + \u8870\u8001\u68c0\u6d4b\uff09\u3002<\/li>\n<li>\u989d\u5916\u9a8c\u8bc1\uff1a\u8868\u578b \/ \u529f\u80fd\u9a8c\u8bc1\u9700\u6839\u636e\u68c0\u6d4b\u9879\u76ee\u989d\u5916\u6536\u8d39\uff08\u5982 Western Blot \u68c0\u6d4b\u86cb\u767d\u8868\u8fbe\uff0cqPCR \u68c0\u6d4b\u57fa\u56e0\u8868\u8fbe\uff09\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u56db\u3001\u5e94\u7528\u8303\u56f4\u4e0e\u516c\u53f8\u4f18\u52bf<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u6838\u5fc3\u5e94\u7528\u573a\u666f<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u57fa\u7840\u7814\u7a76\uff1a\u7ec6\u80de\u589e\u6b96 \/ \u8870\u8001\u673a\u5236\u63a2\u7d22\u3001\u7ec6\u80de\u5206\u5316 \/ \u51cb\u4ea1\u8c03\u63a7\u7814\u7a76\u7684\u4f53\u5916\u6a21\u578b\u3002<\/li>\n<li>\u80bf\u7624\u7814\u7a76\uff1a\u6a21\u62df\u80bf\u7624\u53d1\u751f\u8fc7\u7a0b\uff08\u5982\u6291\u764c\u57fa\u56e0\u5931\u6d3b\u3001\u7aef\u7c92\u9176\u6fc0\u6d3b\uff09\uff0c\u7b5b\u9009\u6297\u80bf\u7624\u836f\u7269\u3002<\/li>\n<li>\u5e94\u7528\u5f00\u53d1\uff1a\u5668\u5b98\u79fb\u690d\u76f8\u5173\u7ec6\u80de\uff08\u5982\u5185\u76ae\u7ec6\u80de\u3001\u5e72\u7ec6\u80de\uff09\u7684\u957f\u671f\u57f9\u517b\uff0c\u751f\u7269\u5236\u5242\u751f\u4ea7\u7684\u7a33\u5b9a\u7ec6\u80de\u6e90\u3002<\/li>\n<\/ul>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u516c\u53f8\u4f18\u52bf<\/h3>\n<ol class=\"auto-hide-last-sibling-br\">\n<li><strong>\u6280\u672f\u6210\u719f<\/strong>\uff1a\u62e5\u6709\u6210\u5957\u6162\u75c5\u6bd2\u7a33\u8f6c\u4f53\u7cfb\uff0c\u652f\u6301\u591a\u79cd\u6c38\u751f\u5316\u65b9\u6cd5\uff0c\u9002\u914d\u4e0d\u540c\u7ec6\u80de\u7c7b\u578b\uff08\u539f\u4ee3\u7ec6\u80de\u3001\u5e72\u7ec6\u80de\u3001\u514d\u75ab\u7ec6\u80de\uff09\u3002<\/li>\n<li><strong>\u7ed3\u679c\u4fdd\u969c<\/strong>\uff1a\u6c38\u751f\u5316\u7ec6\u80de\u682a\u7eaf\u5ea6\u226595%\uff0c\u65e0\u652f\u539f\u4f53 \/ \u7ec6\u83cc\u6c61\u67d3\uff1b\u63d0\u4f9b\u5b8c\u6574\u9274\u5b9a\u62a5\u544a\uff0c\u6570\u636e\u771f\u5b9e\u53ef\u8ffd\u6eaf\u3002<\/li>\n<li><strong>\u6548\u7387\u4e0e\u6210\u672c<\/strong>\uff1a\u4f4e\u6210\u672c\u3001\u9ad8\u6548\u7387\u5236\u5907\u6a21\u5f0f\uff0c4-8 \u5468\u5feb\u901f\u4ea4\u4ed8\uff0c\u6bd4\u884c\u4e1a\u5e73\u5747\u5468\u671f\u7f29\u77ed 1-2 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