{"id":2568,"date":"2025-10-08T14:22:42","date_gmt":"2025-10-08T06:22:42","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=2568"},"modified":"2025-10-08T14:22:42","modified_gmt":"2025-10-08T06:22:42","slug":"%e7%bb%86%e8%83%9e%e7%a8%b3%e8%bd%ac%e6%a0%aa%e6%9e%84%e5%bb%ba%e5%ae%9a%e5%88%b6","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/%e7%bb%86%e8%83%9e%e7%a8%b3%e8%bd%ac%e6%a0%aa%e6%9e%84%e5%bb%ba%e5%ae%9a%e5%88%b6\/","title":{"rendered":"\u7ec6\u80de\u7a33\u8f6c\u682a\u6784\u5efa\u5b9a\u5236"},"content":{"rendered":"<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e00\u3001\u6280\u672f\u6838\u5fc3\u6982\u8ff0<\/h2>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u7ec6\u80de\u7a33\u8f6c\u682a\u662f\u901a\u8fc7\u6162\u75c5\u6bd2\u611f\u67d3 &#8211; \u836f\u7269\u7b5b\u9009\u6cd5\uff0c\u5c06\u5916\u6e90\u57fa\u56e0\u6574\u5408\u81f3\u5bbf\u4e3b\u7ec6\u80de\u67d3\u8272\u4f53 DNA\uff0c\u5b9e\u73b0\u7279\u5b9a\u57fa\u56e0\u957f\u671f\uff08\u22656 \u4e2a\u6708\uff09\u7a33\u5b9a\u8fc7\u8868\u8fbe\u6216\u6c89\u9ed8\u7684\u514b\u9686\u5316\u7ec6\u80de\u7cfb\u3002\u5176\u6838\u5fc3\u4f18\u52bf\u5728\u4e8e\u89e3\u51b3\u4e86\u77ac\u65f6\u8f6c\u67d3\uff08\u8868\u8fbe\u4ec5 2-7 \u5929\u3001\u86cb\u767d\u4ea7\u91cf\u4f4e\uff09\u7684\u5c40\u9650\u6027\uff0c\u53ef\u6ee1\u8db3\u57fa\u56e0\u529f\u80fd\u957f\u671f\u7814\u7a76\u3001\u5de5\u4e1a\u7ea7\u86cb\u767d\u751f\u4ea7\u3001\u836f\u7269\u7b5b\u9009\u7b49\u9700\u6c42\u3002\u76ee\u524d\u4e3b\u6d41\u91c7\u7528<strong>\u4e09\u8d28\u7c92\u6162\u75c5\u6bd2\u7cfb\u7edf<\/strong>\uff08\u5305\u88c5\u8d28\u7c92 psPAX2\u3001\u5305\u819c\u8d28\u7c92 pMD2.G\u3001\u76ee\u7684\u57fa\u56e0\u8d28\u7c92\uff09\u6784\u5efa\uff0c\u9002\u914d\u7edd\u5927\u591a\u6570\u7ec6\u80de\u7cfb\uff0c\u5177\u6709\u6574\u5408\u6548\u7387\u9ad8\u3001\u7ec6\u80de\u9002\u7528\u6027\u5e7f\u7684\u7279\u70b9\u3002<\/div>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e8c\u3001\u6838\u5fc3\u670d\u52a1\u9879\u76ee\u4e0e\u5185\u5bb9<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u670d\u52a1\u9879\u76ee\u5206\u7c7b<\/h3>\n<div class=\"auto-hide-last-sibling-br mdbox-table-root table-container-cYf_5N\" data-scroll-inline-overflow=\"true\" data-scroll-inline-at-start=\"true\" data-scroll-inline-start-overflow=\"false\" data-scroll-inline-at-end=\"false\" data-scroll-inline-end-overflow=\"true\">\n<div class=\"table-scroll-container-Gyf4hQ mdbox-table-scroll-container\">\n<table>\n<thead>\n<tr>\n<th>\u670d\u52a1\u7c7b\u578b<\/th>\n<th>\u6838\u5fc3\u5185\u5bb9<\/th>\n<th>\u9002\u7528\u573a\u666f<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td><strong>\u8fc7\u8868\u8fbe\u7a33\u8f6c\u682a\u6784\u5efa<\/strong><\/td>\n<td>1. \u76ee\u7684\u57fa\u56e0\u8fc7\u8868\u8fbe\u8d28\u7c92\u8bbe\u8ba1\u4e0e\u5236\u5907<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>2. \u6162\u75c5\u6bd2\u5305\u88c5\uff08\u4e09\u8d28\u7c92\u5171\u8f6c 293T \u7ec6\u80de\uff09<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>3. \u6162\u75c5\u6bd2\u611f\u67d3\u76ee\u6807\u7ec6\u80de<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>4. \u6297\u751f\u7d20\u7b5b\u9009\u9633\u6027\u514b\u9686<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>5. qPCR\uff08\u57fa\u56e0\u6c34\u5e73\uff09\u3001WB\uff08\u86cb\u767d\u6c34\u5e73\uff09\u9a8c\u8bc1<\/td>\n<td>\u7814\u7a76\u57fa\u56e0\u529f\u80fd\uff08\u5982\u8fc7\u8868\u8fbe\u67d0\u764c\u57fa\u56e0\u89c2\u5bdf\u7ec6\u80de\u589e\u6b96\u53d8\u5316\uff09\u3001\u91cd\u7ec4\u86cb\u767d\u751f\u4ea7<\/td>\n<\/tr>\n<tr>\n<td><strong>\u5e72\u6270\u7a33\u8f6c\u682a\u5b9a\u5236<\/strong><\/td>\n<td>1. \u9776\u5411\u76ee\u7684\u57fa\u56e0\u7684 shRNA \u5e8f\u5217\u8bbe\u8ba1\u4e0e\u5408\u6210<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>2. \u5e72\u6270\u8d28\u7c92\u6784\u5efa\u4e0e\u6162\u75c5\u6bd2\u5305\u88c5<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>3. \u611f\u67d3\u76ee\u6807\u7ec6\u80de\u540e\u6297\u751f\u7d20\u7b5b\u9009<\/p>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<p>4. qPCR\/WB \u9a8c\u8bc1\u57fa\u56e0\u6c89\u9ed8\u6548\u7387<\/td>\n<td>\u7814\u7a76\u57fa\u56e0\u6572\u964d\u540e\u7ec6\u80de\u8868\u578b\u53d8\u5316\uff08\u5982\u6c89\u9ed8\u6291\u764c\u57fa\u56e0\u89c2\u5bdf\u8fc1\u79fb\u80fd\u529b\uff09<\/td>\n<\/tr>\n<tr>\n<td><strong>\u5e72\u6270\u7a33\u8f6c\u682a\u4e09\u4fdd\u4e00<\/strong><\/td>\n<td>\u5728\u5e72\u6270\u7a33\u8f6c\u682a\u57fa\u7840\u4e0a\uff0c\u63d0\u4f9b 3 \u4e2a\u72ec\u7acb\u6784\u5efa\u7684\u5019\u9009\u514b\u9686\uff0c\u786e\u4fdd\u81f3\u5c11 1 \u4e2a\u514b\u9686\u6ee1\u8db3\u6c89\u9ed8\u6548\u7387\u8981\u6c42\uff08\u901a\u5e38\u226570%\uff09<\/td>\n<td>\u5bf9\u5e72\u6270\u6548\u7387\u8981\u6c42\u9ad8\u3001\u9700\u89c4\u907f\u514b\u9686\u7279\u5f02\u6027\u8bef\u5dee\u7684\u7814\u7a76<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u5173\u952e\u6280\u672f\u7ec6\u8282\uff1a\u836f\u7269\u7b5b\u9009\u4f53\u7cfb<\/h3>\n<div class=\"auto-hide-last-sibling-br paragraph-pP9ZLC paragraph-element br-paragraph-space\">\u4e0d\u540c\u6297\u6027\u6807\u5fd7\u7269\u9002\u914d\u4e0d\u540c\u7b5b\u9009\u9700\u6c42\uff0c\u5177\u4f53\u9009\u62e9\u5982\u4e0b\uff1a<\/div>\n<div class=\"container-Uxvbjy md-box-line-break wrapper-GYqxgQ undefined\"><\/div>\n<div class=\"auto-hide-last-sibling-br mdbox-table-root table-container-cYf_5N\" data-scroll-inline-overflow=\"true\" data-scroll-inline-at-start=\"true\" data-scroll-inline-start-overflow=\"false\" data-scroll-inline-at-end=\"false\" data-scroll-inline-end-overflow=\"true\">\n<div class=\"table-scroll-container-Gyf4hQ mdbox-table-scroll-container\">\n<table>\n<thead>\n<tr>\n<th>\u7b5b\u9009\u836f\u7269<\/th>\n<th>\u4f5c\u7528\u9776\u70b9<\/th>\n<th>\u4f18\u52bf\u5e94\u7528\u573a\u666f<\/th>\n<th>\u7b5b\u9009\u5468\u671f<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u560c\u5464\u9709\u7d20<\/td>\n<td>\u6838\u7cd6\u4f53\u80bd\u57fa\u8f6c\u79fb\u9176<\/td>\n<td>\u5feb\u901f\u7b5b\u9009\uff083-5 \u5929\u89c1\u6548\uff09\uff0c\u9002\u7528\u4e8e\u77ed\u671f\u83b7\u5f97\u9633\u6027\u6df7\u5408\u514b\u9686<\/td>\n<td>3-5 \u5929<\/td>\n<\/tr>\n<tr>\n<td>G418\uff08\u65b0\u9709\u7d20\u7c7b\u4f3c\u7269\uff09<\/td>\n<td>\u5e72\u6270\u86cb\u767d\u8d28\u5408\u6210<\/td>\n<td>\u957f\u5468\u671f\u8868\u8fbe\u9879\u76ee\uff08\u5982\u7a33\u8f6c\u682a\u957f\u671f\u4f20\u4ee3\uff09\uff0c\u7b5b\u9009\u4e25\u8c28\u6027\u4e2d\u7b49<\/td>\n<td>7-10 \u5929<\/td>\n<\/tr>\n<tr>\n<td>\u6f6e\u9709\u7d20<\/td>\n<td>\u86cb\u767d\u8d28\u5408\u6210\u6291\u5236\u5242<\/td>\n<td>\u9ad8\u4e25\u8c28\u6027\u7b5b\u9009\uff08\u5254\u9664\u4f4e\u8868\u8fbe\u514b\u9686\uff09\uff0c\u9002\u7528\u4e8e\u9ad8\u7eaf\u5ea6\u5355\u514b\u9686\u7b5b\u9009<\/td>\n<td>5-7 \u5929<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">3. \u7a33\u8f6c\u682a vs. \u77ac\u65f6\u8f6c\u67d3\uff1a\u6838\u5fc3\u4f18\u52bf\u5bf9\u6bd4<\/h3>\n<div class=\"auto-hide-last-sibling-br mdbox-table-root table-container-cYf_5N\" data-scroll-inline-overflow=\"true\" data-scroll-inline-at-start=\"true\" data-scroll-inline-start-overflow=\"false\" data-scroll-inline-at-end=\"false\" data-scroll-inline-end-overflow=\"true\">\n<div class=\"table-scroll-container-Gyf4hQ mdbox-table-scroll-container\">\n<table>\n<thead>\n<tr>\n<th>\u9700\u6c42\u573a\u666f<\/th>\n<th>\u77ac\u65f6\u8f6c\u67d3<\/th>\n<th>\u7a33\u8f6c\u682a<\/th>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td>\u8868\u8fbe\u6301\u7eed\u65f6\u95f4<\/td>\n<td>\u77ed\u671f\uff082-7 \u5929\uff09\uff0c\u4ec5\u9002\u7528\u4e8e\u521d\u6b65\u9a8c\u8bc1<\/td>\n<td>\u957f\u671f\uff08\u6570\u6708\u81f3\u6570\u5e74\uff09\uff0c\u652f\u6301\u957f\u671f\u673a\u5236\u7814\u7a76<\/td>\n<\/tr>\n<tr>\n<td>\u8868\u8fbe\u7a33\u5b9a\u6027<\/td>\n<td>\u7ec6\u80de\u5206\u88c2\u6613\u7a00\u91ca\uff0c\u8868\u8fbe\u6c34\u5e73\u6ce2\u52a8\u5927<\/td>\n<td>\u57fa\u56e0\u6574\u5408\u81f3\u57fa\u56e0\u7ec4\uff0c\u5355\u514b\u9686\u7b5b\u9009\u540e\u8868\u8fbe\u5747\u4e00<\/td>\n<\/tr>\n<tr>\n<td>\u86cb\u767d\u4ea7\u91cf<\/td>\n<td>\u4f4e\uff08&lt;100mg\/L\uff09\uff0c\u4ec5\u9002\u7528\u4e8e\u5c0f\u91cf\u5236\u5907<\/td>\n<td>\u9ad8\uff08\u53ef\u7b5b\u9009\u9ad8\u8868\u8fbe\u514b\u9686\uff0cCHO \u7ec6\u80de\u53ef\u8fbe g\/L \u7ea7\uff09\uff0c\u9002\u914d\u5de5\u4e1a\u751f\u4ea7<\/td>\n<\/tr>\n<tr>\n<td>\u5b9e\u9a8c\u6210\u672c<\/td>\n<td>\u64cd\u4f5c\u7b80\u5355\u4f46\u9700\u53cd\u590d\u8f6c\u67d3\uff0c\u957f\u671f\u603b\u6210\u672c\u9ad8<\/td>\n<td>\u524d\u671f\u7b5b\u9009\u590d\u6742\uff0c\u540e\u671f\u53ef\u6279\u91cf\u57f9\u517b\uff0c\u957f\u671f\u6210\u672c\u4f4e<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<\/div>\n<\/div>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u4e09\u3001\u670d\u52a1\u6d41\u7a0b\u4e0e\u5468\u671f\u62a5\u4ef7<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u670d\u52a1\u6d41\u7a0b\uff08\u5ba2\u6237 &#8211; \u516c\u53f8\u5bf9\u63a5\uff09<\/h3>\n<ol class=\"auto-hide-last-sibling-br\">\n<li><strong>\u9700\u6c42\u786e\u8ba4<\/strong>\uff1a\u5ba2\u6237\u63d0\u4f9b\u76ee\u6807\u7ec6\u80de\uff08\u9700\u4fdd\u8bc1\u53ef\u4f20\u4ee3\u6b21\u6570\u22655 \u4ee3\uff0c\u65e0\u652f\u539f\u4f53\u6c61\u67d3\uff09\uff0c\u660e\u786e\u670d\u52a1\u7c7b\u578b\uff08\u8fc7\u8868\u8fbe \/ \u5e72\u6270\uff09\u3001\u76ee\u7684\u57fa\u56e0\u4fe1\u606f\u3001\u9a8c\u8bc1\u9700\u6c42\uff08qPCR\/WB\/ \u6d41\u5f0f\uff09\uff1b<\/li>\n<li><strong>\u5b9e\u9a8c\u6267\u884c<\/strong>\uff1a\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u9636\u6bb5 1\uff1a\u8f7d\u4f53\u6784\u5efa\u4e0e\u6162\u75c5\u6bd2\u5305\u88c5\uff081-2 \u5468\uff09\uff1b<\/li>\n<li>\u9636\u6bb5 2\uff1a\u6162\u75c5\u6bd2\u611f\u67d3\u76ee\u6807\u7ec6\u80de + \u6297\u751f\u7d20\u7b5b\u9009\uff081-2 \u5468\uff09\uff1b<\/li>\n<li>\u9636\u6bb5 3\uff1a\u5355\u514b\u9686\u5206\u79bb\uff08\u53ef\u9009\uff09+ \u57fa\u56e0 \/ \u86cb\u767d\u6c34\u5e73\u9a8c\u8bc1\uff081-2 \u5468\uff09\uff1b<\/li>\n<\/ul>\n<\/li>\n<li><strong>\u7ed3\u679c\u4ea4\u4ed8<\/strong>\uff1a\u6309\u7ea6\u5b9a\u63d0\u4f9b\u7a33\u8f6c\u7ec6\u80de\u682a\u53ca\u5b8c\u6574\u62a5\u544a\u3002<\/li>\n<\/ol>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u5468\u671f\u4e0e\u62a5\u4ef7<\/h3>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u670d\u52a1\u5468\u671f<\/strong>\uff1a4-8 \u5468\uff08\u89c6\u7ec6\u80de\u611f\u67d3\u6548\u7387\u3001\u7b5b\u9009\u96be\u5ea6\u8c03\u6574\uff0c\u5355\u514b\u9686\u7b5b\u9009\u9700\u989d\u5916 1-2 \u5468\uff09\uff1b<\/li>\n<li><strong>\u62a5\u4ef7\u65b9\u5f0f<\/strong>\uff1a\u9700\u6839\u636e\u670d\u52a1\u7c7b\u578b\uff08\u8fc7\u8868\u8fbe \/ \u5e72\u6270 \/ \u4e09\u4fdd\u4e00\uff09\u3001\u7ec6\u80de\u7c7b\u578b\uff08\u5e38\u89c4\u7ec6\u80de\u7cfb \/ \u96be\u611f\u67d3\u7ec6\u80de\uff09\u3001\u9a8c\u8bc1\u9879\u76ee\u6570\u91cf\u8be2\u4ef7\uff0c\u5177\u4f53\u53ef\u5bf9\u63a5\u5de5\u4f5c\u4eba\u5458\u6838\u7b97\u3002<\/li>\n<\/ul>\n<h2 class=\"header-iWP5WJ auto-hide-last-sibling-br\">\u56db\u3001\u5b9e\u9a8c\u8981\u6c42\u4e0e\u7ec8\u4ea7\u54c1\u4ea4\u4ed8<\/h2>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">1. \u5ba2\u6237\u9700\u6ee1\u8db3\u7684\u5b9e\u9a8c\u8981\u6c42<\/h3>\n<ol class=\"auto-hide-last-sibling-br\">\n<li>\u76ee\u6807\u7ec6\u80de\uff1a\u9700\u63d0\u4f9b\u5bf9\u6570\u751f\u957f\u671f\u7ec6\u80de\uff08\u6570\u91cf\u22651\u00d710\u2076\u4e2a\uff09\uff0c\u65e0\u652f\u539f\u4f53\u3001\u7ec6\u83cc\u6c61\u67d3\uff0c\u53ef\u4f20\u4ee3\u6b21\u6570\u22655 \u4ee3\uff1b<\/li>\n<li>\u76ee\u7684\u57fa\u56e0\u4fe1\u606f\uff1a\u8fc7\u8868\u8fbe\u9700\u63d0\u4f9b\u57fa\u56e0\u5e8f\u5217\u6216 NCBI \u767b\u5f55\u53f7\uff0c\u5e72\u6270\u9700\u63d0\u4f9b\u9776\u5411\u57fa\u56e0 ID\uff08\u4fbf\u4e8e\u8bbe\u8ba1 shRNA\uff09\uff1b<\/li>\n<li>\u7279\u6b8a\u9700\u6c42\uff1a\u5982\u9700\u8981\u5355\u514b\u9686\u7b5b\u9009\u3001\u6d41\u5f0f\u68c0\u6d4b\u7b49\u989d\u5916\u670d\u52a1\uff0c\u9700\u63d0\u524d\u8bf4\u660e\u3002<\/li>\n<\/ol>\n<h3 class=\"header-iWP5WJ auto-hide-last-sibling-br\">2. \u7ec8\u4ea7\u54c1\u4ea4\u4ed8\u6e05\u5355<\/h3>\n<ol class=\"auto-hide-last-sibling-br\">\n<li>\u7a33\u5b9a\u8868\u8fbe\u7ec6\u80de\u682a\uff1a\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u8fc7\u8868\u8fbe \/ \u5e72\u6270\u7a33\u8f6c\u7ec6\u80de\u682a\uff081\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u542b 2-3 \u4ee3\u7ec6\u80de\uff09\uff1b<\/li>\n<li>\u82e5\u4e3a \u201c\u4e09\u4fdd\u4e00\u201d \u670d\u52a1\uff0c\u63d0\u4f9b 3 \u4e2a\u72ec\u7acb\u5e72\u6270\u514b\u9686\uff1b<\/li>\n<\/ul>\n<\/li>\n<li>\u9a8c\u8bc1\u62a5\u544a\uff1a\n<ul class=\"auto-hide-last-sibling-br\">\n<li>\u57fa\u56e0\u6c34\u5e73\uff1aqPCR \u68c0\u6d4b\u76ee\u7684\u57fa\u56e0\u8868\u8fbe\u91cf\uff08\u8fc7\u8868\u8fbe\u7ec4\u9700\u22655 \u500d\u4e0a\u8c03\uff0c\u5e72\u6270\u7ec4\u9700\u226570% \u4e0b\u8c03\uff09\uff1b<\/li>\n<li>\u86cb\u767d\u6c34\u5e73\uff1aWB 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