{"id":262,"date":"2025-08-24T16:16:07","date_gmt":"2025-08-24T08:16:07","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=262"},"modified":"2025-08-24T16:16:07","modified_gmt":"2025-08-24T08:16:07","slug":"sup-b15%e4%ba%baph%e6%80%a5%e6%b7%8b%e7%99%bd%e8%a1%80%e7%97%85%e7%bb%86%e8%83%9e%e7%b3%bb%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/sup-b15%e4%ba%baph%e6%80%a5%e6%b7%8b%e7%99%bd%e8%a1%80%e7%97%85%e7%bb%86%e8%83%9e%e7%b3%bb%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"SUP-B15\u4ebaPh+\u6025\u6dcb\u767d\u8840\u75c5\u7ec6\u80de\u7cfb\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li 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class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u79cd\u5c5e\u6765\u6e90<\/b>\uff1a\u4eba\uff08STR \u9274\u5b9a\u65e0\u4ea4\u53c9\u6c61\u67d3\uff0c\u79cd\u5c5e\u7eaf\u51c0\uff0c\u4fdd\u969c Ph + \u6025\u6dcb\u7279\u6709\u9057\u4f20\u5b66\u7279\u5f81\u53ca B \u7ec6\u80de\u6807\u5fd7\u7269\u8868\u8fbe\u7a33\u5b9a\uff0c\u4e0e TF-1\u3001Jurkat \u7684\u79cd\u5c5e\u7eaf\u51c0\u6027\u4e00\u81f4\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u6027\u522b\u5e74\u9f84<\/b>\uff1a\u672a\u660e\u786e\uff1b8 \u5c81\uff08\u513f\u7ae5\u9636\u6bb5\uff0c\u586b\u8865 8-10 \u5c81\u513f\u7ae5 Ph + \u6025\u6dcb\u7ec6\u80de\u6a21\u578b\u7a7a\u767d\uff0c\u4e0e TF-1 \u7684 \u201c35 \u5c81\u7537\u201d \u5f62\u6210 \u201c\u513f\u7ae5 &#8211; \u4e2d\u5e74\u201d \u5e74\u9f84\u68af\u5ea6\uff0c\u9002\u914d\u513f\u7ae5\u6025\u6027\u6dcb\u5df4\u6bcd\u7ec6\u80de\u767d\u8840\u75c5\u9ad8\u53d1\u4eba\u7fa4\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec4\u7ec7\u6765\u6e90<\/b>\uff1a\u9aa8\u9ad3\uff1b\u75c5\u7406\u7c7b\u578b\uff1a\u6025\u6027\u6dcb\u5df4\u6bcd\u7ec6\u80de\u767d\u8840\u75c5\uff08B \u6dcb\u5df4\u6bcd\u7ec6\u80de\u578b\uff0cPh+\uff09\uff08\u8840\u6db2\u7cfb\u7edf B \u7ec6\u80de\u80bf\u7624\uff0c\u4e0e TF-1 \u7684\u7ea2\u7cfb\u767d\u8840\u75c5\u3001Jurkat \u7684 T \u7ec6\u80de\u767d\u8840\u75c5\u540c\u5c5e\u767d\u8840\u75c5\u4f46\u4e9a\u578b \/ \u7ec6\u80de\u7cfb\u522b\u4e0d\u540c\uff0c\u4fdd\u7559 B \u7ec6\u80de\u7279\u6709\u7684\u5206\u5316\u6807\u5fd7\u7269\u53ca Ph + \u67d3\u8272\u4f53\u7279\u5f81\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u751f\u957f\u7279\u6027<\/b>\uff1a\u60ac\u6d6e\u751f\u957f\uff08\u968f\u57f9\u517b\u65f6\u95f4\u90e8\u5206\u8d34\u58c1\uff0c\u6838\u5fc3\u5dee\u5f02\u7279\u6027\uff09<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"9\" data-line=\"true\">\u2460 \u500d\u589e\u65f6\u95f4\uff5e20-60 hours\uff08\u8303\u56f4\u6781\u5bbd\uff0c\u9002\u914d\u513f\u7ae5\u767d\u8840\u75c5\u7ec6\u80de\u589e\u6b96\u5f02\u8d28\u6027\uff0c\u5feb\u4e8e TF-1 \u7684 36-72 \u5c0f\u65f6\u4e0b\u9650\uff0c\u4e0e Jurkat \u7684 24-48 \u5c0f\u65f6\u90e8\u5206\u91cd\u53e0\uff09\uff1b\u2461 \u5e38\u89c4\u5448\u60ac\u6d6e\u72b6\u6001\uff0c\u57f9\u517b\u540e\u671f\u7ea6 10%-20% \u7ec6\u80de\u8d34\u58c1\uff0c\u4f20\u4ee3\u65f6\u65e0\u9700\u80f0\u9176\uff0c\u76f4\u63a5\u8f7b\u67d4\u5439\u6253\u8d34\u58c1\u7ec6\u80de\u540e\uff0c\u4e0e\u60ac\u6d6e\u7ec6\u80de\u5408\u5e76\uff0c\u5bc6\u5ea6\u8fbe 1\u00d710\u2076-3\u00d710\u2076cells\/mL \u65f6\u6309 1:3-1:4 \u4f20\u4ee3\u3002<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u5f62\u6001<\/b>\uff1a\u5706\u5f62\u7ec6\u80de\u6837\uff08\u60ac\u6d6e\u7ec6\u80de\u5178\u578b\u5f62\u6001\uff0c\u5448\u89c4\u6574\u5706\u5f62\uff0c\u8d34\u58c1\u7ec6\u80de\u5448\u677e\u6563\u5706\u5f62\u8d34\u9644\uff0c\u4e0e TF-1 \u7684 \u201c\u6dcb\u5df4\u6bcd\u7ec6\u80de\u6837\u201d\u3001Jurkat \u7684 \u201c\u5706\u5f62\uff0c\u5355\u4e2a\u6216\u5448\u7247\u201d \u5f62\u6001\u76f8\u8fd1\uff0c\u53ef\u901a\u8fc7 \u201c\u90e8\u5206\u8d34\u58c1\u201d \u53ca Ph + \u7279\u5f81\u533a\u5206\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u4ee3\u6570<\/b>\uff1a10 \u4ee3\u4ee5\u5185\uff08\u907f\u514d Ph + \u67d3\u8272\u4f53\u4e22\u5931\u3001B \u7ec6\u80de\u6807\u5fd7\u7269\u8868\u8fbe\u4e0b\u964d\u53ca\u8d34\u58c1\u6bd4\u4f8b\u5f02\u5e38\u5347\u9ad8\uff0c\u4e0e TF-1\u3001Jurkat 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class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>STR \u4f4d\u70b9\u4fe1\u606f<\/b>\uff1a\u672a\u5217\u51fa\u5177\u4f53\u4f4d\u70b9\uff0c\u53ef\u901a\u8fc7\u4e0e ATCC CRL-1929\u3001DSMZ ACC-389 \u6807\u51c6\u56fe\u8c31\u6bd4\u5bf9\u786e\u8ba4\u8eab\u4efd\uff0cAmelogenin \u4f4d\u70b9\u53ef\u8865\u5145\u6027\u522b\u4fe1\u606f\uff0c\u4e0e TF-1 \u7684 X,Y \u6838\u578b\u3001Jurkat \u7684\u7537\u6027\u6838\u578b\u5f62\u6210\u6f5c\u5728\u6027\u522b\u533a\u5206\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u89c4\u683c<\/b>\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\uff08\u9002\u914d\u60ac\u6d6e + \u90e8\u5206\u8d34\u58c1\u751f\u957f\uff09\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226580%\uff08\u4e0e TF-1\u3001Jurkat \u7684\u5b58\u6d3b\u7387\u6807\u51c6\u4e00\u81f4\uff0c\u9700\u540c\u65f6\u89c2\u5bdf\u60ac\u6d6e\u53ca\u8d34\u58c1\u7ec6\u80de\u6d3b\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u652f\u539f\u4f53\u68c0\u6d4b<\/b>\uff1a\u65e0\uff08\u907f\u514d\u6c61\u67d3\u5e72\u6270\u7ec6\u80de\u589e\u6b96\u53ca Ph + \u7279\u6027\u68c0\u6d4b\uff0c\u4fdd\u969c\u591a\u7ec6\u80de\u7cfb\u5e73\u884c\u5b9e\u9a8c\u53ef\u9760\u6027\uff0c\u4e0e TF-1\u3001Jurkat \u7684\u68c0\u6d4b\u6807\u51c6\u7edf\u4e00\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u4fdd\u85cf\u673a\u6784<\/b>\uff1aATCC; CRL-1929\uff0cDSMZ; ACC-389\uff08\u591a\u56fd\u9645\u673a\u6784\u4fdd\u85cf\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u4e0e TF-1 \u7684\u591a\u56fd\u9645\u4fdd\u85cf\u3001Jurkat \u7684\u591a\u673a\u6784\u4fdd\u85cf\u5f62\u6210\u4e92\u8865\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u57fa<\/b>\uff1aIMDM+20% FBS+1% PS\uff08\u6838\u5fc3\u914d\u65b9\u5dee\u5f02\uff0c20% \u9ad8\u8840\u6e05\u9002\u914d\u513f\u7ae5\u767d\u8840\u75c5\u7ec6\u80de\u8425\u517b\u9700\u6c42\uff0c\u4e0d\u53ef\u7528 TF-1 \u7684 1640+GM-CSF \u6216 Jurkat \u7684\u57fa\u7840 1640 \u66ff\u4ee3\uff0c\u8840\u6e05\u9700\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u6279\u6b21\uff0c\u5f00\u5c01\u540e 1 \u5468\u5185\u7528\u5b8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u6761\u4ef6<\/b>\uff1a95% \u7a7a\u6c14 + 5% CO\u2082\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff08\u4e0e TF-1\u3001Jurkat 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