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\u6b63\u5e38\u201d \u5e73\u884c\u5b9e\u9a8c\u53ef\u9760\u6027\uff0c\u4e0e HMEC-1\u3001KU812 \u7684\u68c0\u6d4b\u6807\u51c6\u7edf\u4e00\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u4fdd\u85cf\u673a\u6784<\/b>\uff1aATCC; CCL-240\uff0cBCRC; 60027\uff0cBCRJ; 0104\uff0cDSMZ; ACC-3\uff0cECACC; 98070106\uff1b\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u57fa\u7840\u533b\u5b66\u7814\u7a76\u6240\u7ec6\u80de\u8d44\u6e90\u4e2d\u5fc3\uff08\u591a\u56fd\u9645 + \u56fd\u5185\u673a\u6784\u4fdd\u85cf\uff0c\u4e0e HMEC-1 \u7684\u5355\u4e00\u56fd\u9645\u4fdd\u85cf\u3001KU812 \u7684\u591a\u673a\u6784\u4fdd\u85cf\u5f62\u6210\u4e92\u8865\uff0c\u65e9\u5e7c\u7c92\u7279\u6027\u53ca\u5206\u5316\u6f5c\u80fd\u7ecf\u8fc7\u591a\u673a\u6784\u9a8c\u8bc1\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u57fa<\/b>\uff1a80% IMDM+20% FBS +PS\uff08\u6838\u5fc3\u914d\u65b9\u5dee\u5f02\uff0c20% \u9ad8\u8840\u6e05\u4e3a\u5fc5\u9700\u6761\u4ef6\uff0c\u4e0d\u53ef\u7528 HMEC-1 \u7684 ECM \u57f9\u517b\u57fa\u6216 KU812 \u7684\u5e38\u89c4 1640 \u66ff\u4ee3\uff0c\u8840\u6e05\u9700\u9009\u62e9\u80ce\u725b\u8840\u6e05\u4ee5\u652f\u6301\u5206\u5316\u6f5c\u80fd\uff0c\u5f00\u5c01\u540e 1 \u5468\u5185\u7528\u5b8c\uff0c\u9632\u6b62\u8425\u517b\u6210\u5206\u964d\u89e3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u6761\u4ef6<\/b>\uff1a95% \u7a7a\u6c14 + 5% CO\u2082\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff08\u4e0e HMEC-1\u3001KU812 \u57f9\u517b\u73af\u5883\u53c2\u6570\u4e00\u81f4\uff0c\u65e0\u9700\u989d\u5916\u8c03\u6574\uff0c\u4f46\u56e0\u9ad8\u8840\u6e05\u6613\u6ecb\u751f\u6c61\u67d3\uff0c\u57f9\u517b\u7bb1\u9700\u5b9a\u671f\u6d88\u6bd2\uff0c\u540c\u65f6\u7a33\u5b9a\u63a7\u6e29\uff08\u6ce2\u52a8\u2264\u00b10.3\u2103\uff09\uff0c\u907f\u514d\u6e29\u5ea6\u5f71\u54cd\u7ec6\u80de\u589e\u6b96\u53ca\u5206\u5316\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u51bb\u5b58\u6761\u4ef6<\/b>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff084\u00d710\u2076cells\/mL\uff0c\u9ad8\u4e8e\u5e38\u89c4\u60ac\u6d6e\u7ec6\u80de\u6d53\u5ea6\uff0c4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 2 \u5c0f\u65f6\u2192-80\u2103\u2192\u6db2\u6c2e\uff0c\u9ad8\u8840\u6e05\u57f9\u517b\u7ec6\u80de\u51bb\u5b58\u5bc6\u5ea6\u9700\u66f4\u9ad8\uff0c\u590d\u82cf\u65f6\u53ef\u6dfb\u52a0 10% FBS \u63d0\u5347\u5b58\u6d3b\u7387\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u81f4\u7624\u6027<\/b>\uff1aYes, in nude mice (subcutaneous myeloid tumors). Yes, in semi-solid media\uff08\u88f8\u9f20\u76ae\u4e0b\u81f4\u7624 + \u534a\u56fa\u4f53\u57f9\u517b\u57fa\u6210\u7624\uff0c\u4e0e KU812 \u7684\u672a\u660e\u786e\u81f4\u7624\u6a21\u578b\u3001HMEC-1 \u7684\u65e0\u81f4\u7624\u6027\u5f62\u6210\u4e92\u8865\uff0c\u6210\u7624\u5468\u671f\u63a8\u6d4b 14-21 \u5929\uff0c\u9002\u5408\u6025\u6027\u65e9\u5e7c\u7c92\u767d\u8840\u75c5\u4f53\u5185\u81f4\u7624\u673a\u5236\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u53d7\u4f53 \/ \u57fa\u56e0\u8868\u8fbe<\/b>\uff1a\u2460 \u53d7\u4f53\uff1a\u8865\u4f53\u53d7\u4f53\u3001Fc \u53d7\u4f53\u8868\u8fbe\uff08\u4e0e\u541e\u566c\u6d3b\u6027\u3001\u8d8b\u5316\u54cd\u5e94\u76f8\u5173\uff0c\u53ef\u901a\u8fc7\u6d41\u5f0f\u9a8c\u8bc1\uff09\uff1b\u2461 \u57fa\u56e0\uff1amyc+\uff0cPMA \u523a\u6fc0\u540e\u8868\u8fbe TNF-\u03b1\uff08\u53ef\u901a\u8fc7 RT-PCR \u9a8c\u8bc1\uff0c\u533a\u522b\u4e8e KU812 \u7684\u7ec4\u80fa\u751f\u6210\u76f8\u5173\u57fa\u56e0\u3001HMEC-1 \u7684\u5185\u76ae\u6807\u5fd7\u7269\u57fa\u56e0\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u67d3\u8272\u4f53<\/b>\uff1a34~43\uff08\u4f4e\u5f02\u500d\u4f53\uff0c\u67d3\u8272\u4f53\u6570\u76ee\u4f4e\u4e8e\u6b63\u5e38\u4e8c\u500d\u4f53\uff0c\u6bcf 5 \u4ee3\u901a\u8fc7\u6838\u578b\u5206\u6790\u9a8c\u8bc1\uff0c\u786e\u4fdd\u4e0e\u65e9\u5e7c\u7c92\u767d\u8840\u75c5\u76f8\u5173\u7684\u9057\u4f20\u7279\u5f81\u7a33\u5b9a\uff0c\u533a\u522b\u4e8e KU812 \u7684\u672a\u660e\u786e\u6838\u578b\u3001HMEC-1 \u7684\u6b63\u5e38\u6838\u578b\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u8d27\u671f<\/b>\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u8fd0\u8f93\u65b9\u5f0f<\/b>\uff1a\u590d\u82cf\u514d\u8fd0\u8d39\uff1b\u51bb\u5b58\u9700\u52a0\u5e72\u51b0\u8d39<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u4f9b\u5e94\u9650\u5236<\/b>\uff1a\u4ec5\u9650\u79d1\u7814\uff0c\u7981\u6b62\u4e34\u5e8a\u4f7f\u7528\uff08\u4e0e HMEC-1\u3001KU812 \u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e0d\u53ef\u7528\u4e8e\u6025\u6027\u65e9\u5e7c\u7c92\u767d\u8840\u75c5\u4e34\u5e8a\u6cbb\u7597\u76f8\u5173\u5b9e\u9a8c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7279\u522b\u8bf4\u660e<\/b>\uff1a\u2460 \u5206\u5316\u9a8c\u8bc1\uff1a\u9996\u6b21\u57f9\u517b\u53ef\u7528 1.25% DMSO \u8bf1\u5bfc 72 \u5c0f\u65f6\uff0c\u901a\u8fc7\u745e\u6c0f\u67d3\u8272\u89c2\u5bdf\u7ec6\u80de\u5f62\u6001\uff08\u65e9\u5e7c\u7c92\u2192\u4e2d\u5e7c\u7c92 \/ \u665a\u5e7c\u7c92\uff09\uff0c\u6216\u68c0\u6d4b CD11b\uff08\u5206\u5316\u6807\u5fd7\u7269\uff09\u9633\u6027\u7387\uff08\u226580%\uff09\uff0c\u786e\u8ba4\u5206\u5316\u6f5c\u80fd\uff1b\u2461 \u57f9\u517b\u6ce8\u610f\uff1a\u9ad8\u8840\u6e05\u57f9\u517b\u57fa\u9700\u907f\u5149\u50a8\u5b58\uff0c\u4f20\u4ee3\u65f6\u907f\u514d\u8fc7\u5ea6\u7a00\u91ca\u5bfc\u81f4\u7ec6\u80de\u8425\u517b\u4e0d\u8db3\uff0c\u5efa\u8bae\u6bcf\u6b21\u4f20\u4ee3\u540e\u8865\u5145\u65b0\u9c9c FBS\uff1b\u2462 \u5b9e\u9a8c\u9002\u914d\uff1a\u9002\u5408\u5206\u5316\u8bf1\u5bfc\u836f\u7269\uff08\u5982\u7ef4 A \u9178\u3001\u4e09\u6c27\u5316\u4e8c\u7837\uff09\u7b5b\u9009\u3001myc \u57fa\u56e0\u529f\u80fd\u7814\u7a76\u3001\u80bf\u7624\u7ec6\u80de\u541e\u566c\u673a\u5236\u5b9e\u9a8c\uff0c\u53ef\u4e0e KU812 \u6784\u5efa \u201c\u9ad3\u7cfb\u4e0d\u540c\u4e9a\u578b\u201d \u5bf9\u6bd4\u6a21\u578b\uff0c\u63a2\u7d22\u5206\u5316\u8bf1\u5bfc\u6cbb\u7597\u7684\u4e9a\u578b\u9009\u62e9\u6027\uff0c\u6216\u4e0e HMEC-1 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