{"id":291,"date":"2025-08-24T19:05:40","date_gmt":"2025-08-24T11:05:40","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=291"},"modified":"2025-08-24T19:05:41","modified_gmt":"2025-08-24T11:05:41","slug":"ln229%e4%ba%ba%e5%a4%a7%e8%84%91%e7%a5%9e%e7%bb%8f%e6%af%8d%e7%98%a4%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/ln229%e4%ba%ba%e5%a4%a7%e8%84%91%e7%a5%9e%e7%bb%8f%e6%af%8d%e7%98%a4%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"LN229\u4eba\u5927\u8111\u795e\u7ecf\u6bcd\u7624\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li 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data-list=\"bullet1\"><b>\u7ec6\u80de\u89c4\u683c<\/b>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226580%\uff08\u4e0e U-87 MG\u3001HEB \u7684\u5b58\u6d3b\u7387\u6807\u51c6\u4e00\u81f4\uff0c\u4f4e\u8840\u6e05\u7ec6\u80de\u590d\u82cf\u540e\u9002\u5e94\u8f83\u6162\uff0c\u9700 48 \u5c0f\u65f6\u89c2\u5bdf\u8d34\u58c1\u7387\uff08\u5e94\u226585%\uff09\u53ca\u4e0a\u76ae\u5f62\u6001\u6062\u590d\u60c5\u51b5\uff0c\u540c\u6b65\u786e\u8ba4\u57fa\u56e0\u7a81\u53d8\u7a33\u5b9a\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u652f\u539f\u4f53\u68c0\u6d4b<\/b>\uff1a\u65e0\uff08\u907f\u514d\u6c61\u67d3\u5e72\u6270\u795e\u7ecf\u6bcd\u7624\u589e\u6b96\u53ca\u57fa\u56e0\u7a81\u53d8\u68c0\u6d4b\uff0c\u4fdd\u969c \u201c\u4f4e\u8840\u6e05 &#8211; \u5e38\u89c4\u8840\u6e05\u201d\u201c\u7a81\u53d8 &#8211; \u65e0\u7a81\u53d8\u201d 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\u4f4e\u8840\u6e05\u4e3a\u5173\u952e\u7279\u5f81\uff0c\u533a\u522b\u4e8e U-87 MG \u7684 \u201c90% DMEM+10% FBS\u201d\u3001HEB \u7684 \u201cDMEM+10% FBS\u201d\uff0c\u4e0d\u53ef\u968f\u610f\u63d0\u9ad8\u8840\u6e05\u6d53\u5ea6\uff08\u6613\u5bfc\u81f4\u57fa\u56e0\u7a81\u53d8\u8868\u8fbe\u5f02\u5e38\uff09\uff0c\u8840\u6e05\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u6279\u6b21\uff08\u226410EU\/mL\uff09\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c\u4f4e\u8840\u6e05\u57f9\u517b\u57fa\u9700\u907f\u5149\u50a8\u5b58\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u6761\u4ef6<\/b>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff08\u4e0e U-87 MG\u3001HEB \u57f9\u517b\u73af\u5883\u53c2\u6570\u4e00\u81f4\uff0c\u65e0\u9700\u989d\u5916\u8c03\u6574\uff0c\u4f4e\u8840\u6e05\u7ec6\u80de\u5bf9\u6e7f\u5ea6\u66f4\u654f\u611f\uff0c\u57f9\u517b\u7bb1\u6e7f\u5ea6\u9700\u7a33\u5b9a\u5728 75%\u00b15%\uff0c\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u8840\u6e05\u6d53\u5ea6\u5f02\u5e38\uff0c\u5f71\u54cd\u7ec6\u80de\u6d3b\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u51bb\u5b58\u6761\u4ef6<\/b>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff082\u00d710\u2076cells\/mL\uff0c\u9002\u914d\u8d34\u58c1\u7ec6\u80de\u5bc6\u5ea6\u9700\u6c42\uff0c4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 3 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u50a8\u5b58\uff0c\u51bb\u5b58\u524d\u9700\u5728\u542b 5% FBS \u7684\u57f9\u517b\u57fa\u4e2d\u57f9\u517b 24 \u5c0f\u65f6\uff08\u8ba9\u7ec6\u80de\u9002\u5e94\u8840\u6e05\u73af\u5883\uff09\uff0c\u63d0\u5347\u590d\u82cf\u5b58\u6d3b\u7387\uff0c\u907f\u514d\u76f4\u63a5\u51bb\u5b58\u4f4e\u8840\u6e05\u57f9\u517b\u7684\u5bf9\u6570\u671f\u7ec6\u80de\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u5206\u5b50\u7279\u5f81<\/b>\uff1a\u2460 p53 \u7a81\u53d8\uff08\u80bf\u7624\u5e38\u89c1\u9a71\u52a8\u7a81\u53d8\uff0c\u53ef\u901a\u8fc7\u6d4b\u5e8f\u6216 Western Blot \u9a8c\u8bc1\u7a81\u53d8\u578b\u86cb\u767d\u8868\u8fbe\uff09\uff1b\u2461 p16 \u53ca p14ARF \u7f3a\u5931\uff08\u80bf\u7624\u6291\u5236\u57fa\u56e0\u7f3a\u5931\uff0c\u53ef\u901a\u8fc7 PCR \u6216\u514d\u75ab\u7ec4\u5316\u68c0\u6d4b\u57fa\u56e0 \/ \u86cb\u767d\u8868\u8fbe\u7f3a\u5931\uff09\uff1b\u2462 \u91ce\u751f\u578b PTEN\uff08\u78f7\u9178\u9176\u57fa\u56e0\uff0c\u53ef\u901a\u8fc7\u6d4b\u5e8f\u786e\u8ba4\u65e0\u7a81\u53d8\uff0c\u533a\u522b\u4e8e U-87 MG \u7684\u672a\u660e\u786e PTEN \u72b6\u6001\u3001HEB \u7684\u6b63\u5e38 PTEN\uff09\uff0c\u8fd9\u4e9b\u5206\u5b50\u7279\u5f81\u662f\u7814\u7a76\u80bf\u7624\u53d1\u751f\u673a\u5236\u53ca\u9776\u5411\u836f\u7269\u7b5b\u9009\u7684\u6838\u5fc3\u9776\u70b9<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u8d27\u671f<\/b>\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u8fd0\u8f93\u65b9\u5f0f<\/b>\uff1a\u590d\u82cf\u514d\u8fd0\u8d39\uff1b\u51bb\u5b58\u9700\u52a0\u5e72\u51b0\u8d39<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u4f9b\u5e94\u9650\u5236<\/b>\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e U-87 MG\u3001HEB \u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u4e0d\u53ef\u7528\u4e8e\u795e\u7ecf\u6bcd\u7ec6\u80de\u7624\u4e34\u5e8a\u6cbb\u7597\u76f8\u5173\u5b9e\u9a8c\uff0c\u867d\u65e0\u660e\u786e\u4f53\u5185\u6210\u7624\u6027\u63cf\u8ff0\u4f46\u5c5e\u6076\u6027\u80bf\u7624\u7ec6\u80de\uff0c\u4e25\u7981\u7528\u4e8e\u4eba\u4f53\u76f8\u5173\u5e72\u9884\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7279\u522b\u8bf4\u660e<\/b>\uff1a\u2460 \u8840\u6e05\u63a7\u5236\uff1a\u9996\u6b21\u57f9\u517b\u9700\u4e25\u683c\u7ef4\u6301 5% \u8840\u6e05\u6d53\u5ea6\uff0c\u4e0d\u53ef\u6dfb\u52a0\u9ad8\u4e8e 5% \u7684\u8840\u6e05\uff08\u6613\u8bf1\u5bfc\u7ec6\u80de\u5f62\u6001\u7ea4\u7ef4\u5316\u53ca\u57fa\u56e0\u7a81\u53d8\u8868\u8fbe\u5f02\u5e38\uff09\uff0c\u5efa\u8bae\u4f7f\u7528\u540c\u4e00\u6279\u6b21\u8840\u6e05\u4ee5\u4fdd\u8bc1\u5b9e\u9a8c\u91cd\u590d\u6027\uff1b\u2461 \u5206\u5b50\u9a8c\u8bc1\uff1a\u6bcf 5 \u4ee3\u901a\u8fc7 Sanger \u6d4b\u5e8f\u9a8c\u8bc1 p53 \u7a81\u53d8\u53ca PTEN \u91ce\u751f\u578b\u72b6\u6001\uff0c\u901a\u8fc7 Western Blot \u68c0\u6d4b p16\/p14ARF \u86cb\u767d\u8868\u8fbe\uff08\u786e\u8ba4\u7f3a\u5931\uff09\uff0c\u786e\u4fdd\u5206\u5b50\u7279\u5f81\u7a33\u5b9a\uff1b\u2462 \u57f9\u517b\u6ce8\u610f\uff1a\u4f4e\u8840\u6e05\u57f9\u517b\u57fa\u8425\u517b\u6709\u9650\uff0c\u9700\u6bcf 48 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