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data-list=\"bullet1\"><b>\u4fdd\u85cf\u673a\u6784<\/b>\uff1aDSMZ; ACC-325\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\u4fdd\u85cf\uff0c\u80f6\u8d28\u7624\u7279\u6027\u53ca\u77ed\u500d\u589e\u72b6\u6001\u7ecf DSMZ \u9a8c\u8bc1\uff0c\u4e0e LN229 \u7684 ATCC; CRL-2611\u3001U-87 MG \u7684\u591a\u673a\u6784\u4fdd\u85cf\u5f62\u6210\u4e92\u8865\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u8001\u5e74\u8111\u80bf\u7624\u5b9e\u9a8c\u7684\u7ec6\u80de\u8eab\u4efd\u786e\u8ba4\uff0c\u786e\u4fdd\u7ec6\u80de\u6765\u6e90\u5408\u89c4\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u57fa<\/b>\uff1a89% DMEM+10% FBS+1% PS\uff08\u6838\u5fc3\u914d\u65b9\u5dee\u5f02\uff0c10% \u5e38\u89c4\u8840\u6e05\uff0c\u533a\u522b\u4e8e LN229 \u7684 \u201c5% \u4f4e\u8840\u6e05\u201d\uff0c\u4e0e U-87 MG \u7684 \u201c90% DMEM+10% FBS\u201d \u914d\u65b9\u76f8\u8fd1\uff08\u4ec5\u57fa\u7840\u57f9\u517b\u57fa\u6bd4\u4f8b\u5dee 1%\uff09\uff0c\u53ef\u4e0e U-87 MG \u5171\u7528\u57f9\u517b\u57fa\u964d\u4f4e\u6210\u672c\uff1b\u8840\u6e05\u9009\u62e9\u80ce\u725b\u8840\u6e05\uff08\u4f4e\u5185\u6bd2\u7d20\u226410EU\/mL\uff09\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c\u65e0\u9700\u50cf LN229 \u90a3\u6837\u907f\u5149\u50a8\u5b58\uff0c\u64cd\u4f5c\u66f4\u4fbf\u6377\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u6761\u4ef6<\/b>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff08\u4e0e LN229\u3001U-87 MG \u57f9\u517b\u73af\u5883\u53c2\u6570\u4e00\u81f4\uff0c\u65e0\u9700\u989d\u5916\u8c03\u6574\uff1b\u5feb\u901f\u589e\u6b96\u7ec6\u80de\u5bf9\u8425\u517b\u6d88\u8017\u5feb\uff0c\u9700\u6bcf 24-36 \u5c0f\u65f6\u66f4\u6362 1 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