{"id":296,"date":"2025-08-25T21:20:07","date_gmt":"2025-08-25T13:20:07","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=296"},"modified":"2025-08-25T21:20:29","modified_gmt":"2025-08-25T13:20:29","slug":"wi-38%e4%ba%ba%e8%83%9a%e8%82%ba%e6%88%90%e7%ba%a4%e7%bb%b4%e7%bb%86%e8%83%9e","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/wi-38%e4%ba%ba%e8%83%9a%e8%82%ba%e6%88%90%e7%ba%a4%e7%bb%b4%e7%bb%86%e8%83%9e\/","title":{"rendered":"WI-38\u4eba\u80da\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u540d\u79f0<\/b>\uff1aWI-38 \u4eba\u80da\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"2\" data-line=\"true\">\u6838\u5fc3\u7279\u8272\uff1a\u598a\u5a20 3 \u4e2a\u6708\u5973\u6027\u80da\u80ce\u6b63\u5e38\u80ba\u7ec4\u7ec7\u6765\u6e90 + \u8d34\u58c1\u751f\u957f + \u6210\u7ea4\u7ef4\u7ec6\u80de\u5f62\u6001 + MEM+10% FBS+1% \u53cc\u6297\u57f9\u517b\u57fa +~24 \u5c0f\u65f6\u77ed\u500d\u589e + \u9996\u682a\u75ab\u82d7\u5236\u5907\u7528\u4e8c\u500d\u4f53\u7ec6\u80de\u7cfb + TNF alpha \u4fc3\u751f\u957f + 10 \u4ee3\u4ee5\u5185\u4ee3\u6570\uff0c\u53ef\u4e0e MRC-5\uff0814 \u5468\u9f84\u7537\u80da\u80ba \/ MEM\/36-96h \u500d\u589e \/ 46,XY\uff09\u3001JEG-3\uff08\u7537\u6027\u7ed2\u6bdb\u819c\u764c \/ MEM\/24-36h \u500d\u589e \/ \u8d85\u4e09\u500d\u4f53\uff09\u7ec4\u6210 \u201c\u7ec6\u80de\u7c7b\u578b\uff08\u6b63\u5e38\u80da\u80ba\u7ec6\u80de &#8211; \u6b63\u5e38\u80da\u80ba\u7ec6\u80de &#8211; \u6ecb\u517b\u5c42\u80bf\u7624\uff09+ \u6027\u522b\u80ce\u9f84\uff08\u5973 \/ 3 \u6708 &#8211; \u7537 \/ 14 \u5468 &#8211; \u7537 \/ \u65e0\uff09+ \u529f\u80fd\u7279\u6027\uff08\u75ab\u82d7\u5236\u5907 \/ TNF \u4fc3\u751f\u957f &#8211; \u6b63\u5e38\u589e\u6b96 &#8211; \u6fc0\u7d20\u5206\u6ccc\uff09+ \u500d\u589e\u5dee\u5f02\uff0824h-36-96h-24-36h\uff09+ \u9057\u4f20\u7279\u5f81\uff08\u4e8c\u500d\u4f53 &#8211; \u4e8c\u500d\u4f53 &#8211; \u8d85\u4e09\u500d\u4f53\uff09\u201d \u5bf9\u6bd4\u4f53\u7cfb\uff0c\u7528\u4e8e\u80da\u80ce\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u673a\u5236\u3001\u75ab\u82d7\u5236\u5907\u7ec6\u80de\u6a21\u578b\u7279\u6027\u3001TNF alpha \u8c03\u63a7\u673a\u5236\u53ca\u6b63\u5e38\u7ec6\u80de\u6027\u522b \/ \u80ce\u9f84\u5dee\u5f02\u7814\u7a76\u3002<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u522b\u79f0<\/b>\uff1aWi-38; WI 38; WI38; Wistar Institute-38; AG06814-J; AG06814-M; AG06814-N\uff1b\u4eba\u80da\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"4\" data-line=\"true\">\u6807\u6ce8 \u201c\u5973\u80da\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de + \u75ab\u82d7\u5236\u5907\u7528 + TNF \u4fc3\u751f\u957f + \u77ed\u500d\u589e\u201d\uff0c\u533a\u522b\u4e8e MRC-5 \u7684 \u201c\u7537\u80da\u80ba + \u6b63\u5e38\u4e8c\u500d\u4f53 + \u5bbd\u500d\u589e\u201d\u3001JEG-3 \u7684 \u201c\u7ed2\u6bdb\u819c\u764c + \u6fc0\u7d20\u5206\u6ccc + \u8d85\u4e09\u500d\u4f53\u201d \u6807\u8bc6\uff0c\u51f8\u663e\u5973\u6027\u80da\u80ce\u5c5e\u6027\u53ca\u75ab\u82d7\u5e94\u7528\u4ef7\u503c\uff0c\u89c4\u907f\u4e0e\u540c\u7c7b\u6b63\u5e38\u80ba\u7ec6\u80de\u7684\u6027\u522b \/ \u529f\u80fd\u6df7\u6dc6\u3002<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u79cd\u5c5e\u6765\u6e90<\/b>\uff1a\u4eba\uff08\u65e0\u4ea4\u53c9\u6c61\u67d3\u98ce\u9669\uff0c\u79cd\u5c5e\u7eaf\u51c0\uff0c\u4fdd\u969c\u6b63\u5e38\u80da\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7279\u6709\u7684\u8d34\u58c1\u80fd\u529b\u3001\u6210\u7ea4\u7ef4\u5f62\u6001\u3001MEM \u57f9\u517b\u57fa\u9002\u5e94\u80fd\u529b\u3001\u4e8c\u500d\u4f53\u6838\u578b\u53ca TNF alpha \u54cd\u5e94\u6027\u7a33\u5b9a\uff0c\u4e0e MRC-5\u3001JEG-3 \u7684\u79cd\u5c5e\u6765\u6e90\u4e00\u81f4\uff0c\u65e0\u80bf\u7624\u7ec6\u80de\u6076\u6027\u7279\u5f81\uff0c\u5177\u75ab\u82d7\u5236\u5907\u5408\u89c4\u6027\uff0c\u9002\u914d\u75ab\u82d7\u7814\u53d1\u3001\u836f\u7269\u6bd2\u6027\u5bf9\u7167\u53ca\u80da\u80ce\u53d1\u80b2\u76f8\u5173\u5b9e\u9a8c\u573a\u666f\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u6027\u522b\u5e74\u9f84<\/b>\uff1a\u5973\uff1b\u598a\u5a20 3 \u4e2a\u6708\uff08\u65e9\u671f\u80da\u80ce\u9636\u6bb5\uff0c\u586b\u8865\u5973\u6027\u65e9\u671f\u80da\u80ce\u80ba\u7ec6\u80de\u6a21\u578b\u7a7a\u767d\uff0c\u4e0e MRC-5 \u7684 \u201c14 \u5468\u9f84\u7537\u201d \u5f62\u6210 \u201c\u5973 &#8211; \u7537\u201d \u6027\u522b\u5dee\u5f02\u3001\u201c3 \u6708 &#8211; 14 \u5468\u201d \u80ce\u9f84\u68af\u5ea6\uff083 \u6708\u224812 \u5468\uff0c\u63a5\u8fd1\u4f46\u7565\u65e9\uff09\uff0c\u4e0e JEG-3 \u7684 \u201c\u7537\u6027\u65e0\u660e\u786e\u5e74\u9f84\u201d \u5f62\u6210 \u201c\u5973\u6027\u80da\u80ce &#8211; \u7537\u6027\u80bf\u7624\u201d \u5bf9\u6bd4\uff0c\u9002\u914d\u5973\u6027\u80da\u80ce\u80ba\u53d1\u80b2\u673a\u5236\u3001\u6027\u522b\u76f8\u5173\u80ba\u75be\u75c5\u57fa\u7840\u7814\u7a76\uff0c\u5c24\u5176\u9002\u5408\u75ab\u82d7\u751f\u4ea7\u5de5\u827a\u9a8c\u8bc1\u53ca\u836f\u7269\u5b89\u5168\u6027\u8bc4\u4ef7\u7684\u6b63\u5e38\u7ec6\u80de\u5bf9\u7167\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec4\u7ec7\u6765\u6e90<\/b>\uff1a\u6b63\u5e38\u80ba\uff08\u65e9\u671f\u80da\u80ce\u80ba\u7ec4\u7ec7\uff0c\u4e0e MRC-5 \u7684 \u201c14 \u5468\u9f84\u80ce\u513f\u80ba\u201d \u540c\u5c5e\u6b63\u5e38\u80da\u80ba\u4f46\u80ce\u9f84 \/ \u6027\u522b\u4e0d\u540c\uff0c\u533a\u522b\u4e8e JEG-3 \u7684 \u201c\u7ed2\u6bdb\u819c\u764c\u201d\uff0c\u4fdd\u7559\u80da\u80ce\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7279\u6709\u7684\u589e\u6b96\u5206\u5316\u6f5c\u529b\u53ca\u75ab\u82d7\u5236\u5907\u9002\u914d\u6027\uff0c\u65e0 MRC \u7684\u90e8\u5206\u4e9a\u4e8c\u500d\u4f53\u7279\u5f81\uff0c\u65b0\u589e TNF alpha \u4fc3\u751f\u957f\u7279\u6027\uff0c\u9002\u914d\u75ab\u82d7\u751f\u4ea7\u53ca\u7ec6\u80de\u56e0\u5b50\u8c03\u63a7\u673a\u5236\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u751f\u957f\u7279\u6027<\/b>\uff1a\u8d34\u58c1\u751f\u957f\uff08\u6838\u5fc3\u751f\u957f\u7279\u6027\u4e0e MRC-5\u3001JEG-3 \u4e00\u81f4\uff0c\u4fbf\u4e8e\u5e73\u884c\u5b9e\u9a8c\u64cd\u4f5c\uff09<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"9\" data-line=\"true\">\u2460 \u500d\u589e\u65f6\u95f4\uff5e24 \u5c0f\u65f6\uff08\u660e\u786e\u77ed\u500d\u589e\uff0c\u8fdc\u5feb\u4e8e MRC-5 \u7684 36-96 \u5c0f\u65f6\uff0c\u4e0e JEG-3 \u7684 24-36 \u5c0f\u65f6\u4e0b\u9650\u6301\u5e73\uff0c\u9002\u914d\u65e9\u671f\u80da\u80ce\u7ec6\u80de\u5feb\u901f\u589e\u6b96\u8282\u594f\uff0c\u75ab\u82d7\u751f\u4ea7\u53ca\u5b9e\u9a8c\u5468\u671f\u66f4\u77ed\uff09\uff1b\u2461 \u9700\u80f0\u9176\u6d88\u5316\uff080.25% \u80f0\u9176 + 0.02% EDTA\uff0c37\u2103\u5b75\u80b2 4-6 \u5206\u949f\uff09\uff0c\u7ec6\u80de\u6c47\u5408\u5ea6\u8fbe 75%-85% \u65f6\u6309 1:3-1:4 \u4f20\u4ee3\uff0c\u56e0\u6210\u7ea4\u7ef4\u7ec6\u80de\u5f62\u6001\u8d34\u58c1\u7d27\u5bc6\u4f46\u77ed\u500d\u589e\u9700\u9ad8\u9891\u4f20\u4ee3\uff0c\u6d88\u5316\u65f6\u95f4\u9700\u77ed\u4e8e MRC-5\uff08\u907f\u514d\u8fc7\u5ea6\u6d88\u5316\uff09\uff1b\u4f20\u4ee3\u540e 24 \u5c0f\u65f6\u5185\u8d34\u58c1\u7a33\u5b9a\uff0c\u6dfb\u52a0 TNF alpha\uff08\u63a8\u8350\u6d53\u5ea6 10ng\/mL\uff09\u53ef\u4f7f\u500d\u589e\u65f6\u95f4\u7f29\u77ed\u81f3 20 \u5c0f\u65f6\u5de6\u53f3\uff0c\u589e\u6b96\u6548\u7387\u663e\u8457\u9ad8\u4e8e MRC-5\u3002<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u5f62\u6001<\/b>\uff1a\u6210\u7ea4\u7ef4\u7ec6\u80de\uff08\u6838\u5fc3\u5f62\u6001\u5dee\u5f02\uff0c\u5448\u5178\u578b\u68ad\u5f62\uff0c\u80de\u8d28\u5ef6\u4f38\u5f62\u6210\u5747\u5300\u7ec6\u957f\u7a81\u8d77\uff0c\u6392\u5217\u5448\u5e73\u884c\u6216\u6f29\u6da1\u72b6\uff0c\u4e0e MRC-5 \u7684 \u201c\u6210\u7ea4\u7ef4\u7ec6\u80de\u6837\uff08\u68ad\u5f62 + \u653e\u5c04\u72b6\u6392\u5217\uff09\u201d \u5f62\u6001\u76f8\u8fd1\uff0c\u533a\u522b\u4e8e JEG-3 \u7684 \u201c\u4e0a\u76ae\u6837\u7ec6\u80de\uff08\u591a\u8fb9\u5f62 + \u5206\u6ccc\u9897\u7c92\uff09\u201d\uff0c\u53ef\u901a\u8fc7 \u201c\u5973\u6027\u80da\u80ce\u6765\u6e90 + TNF \u4fc3\u751f\u957f + \u75ab\u82d7\u5e94\u7528\u201d \u5feb\u901f\u9274\u522b\uff0c\u65e9\u671f\u80da\u80ce\u6765\u6e90\u4f7f\u5176\u5f62\u6001\u66f4\u89c4\u6574\u3001\u5927\u5c0f\u66f4\u5747\u4e00\uff0c\u9002\u914d\u75ab\u82d7\u751f\u4ea7\u7684\u7ec6\u80de\u5747\u4e00\u6027\u8981\u6c42\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u4ee3\u6570<\/b>\uff1a10 \u4ee3\u4ee5\u5185\uff08\u907f\u514d\u8d34\u58c1\u80fd\u529b\u4e0b\u964d\u3001\u6210\u7ea4\u7ef4\u5f62\u6001\u6f02\u79fb\u3001\u4e8c\u500d\u4f53\u6838\u578b\u5f02\u5e38\u53ca TNF alpha \u54cd\u5e94\u6027\u4e22\u5931\uff0c\u4e0e MRC-5\u3001JEG-3 \u7684\u4ee3\u6570\u8981\u6c42\u4e00\u81f4\uff1b\u56e0\u65e9\u671f\u80da\u80ce\u7ec6\u80de\u6613\u8001\u5316\u4e14\u6d89\u53ca\u75ab\u82d7\u5e94\u7528\uff0c\u4ee3\u6570\u63a7\u5236\u66f4\u4e25\u683c\uff0c\u9700\u6bcf 4 \u4ee3\u9a8c\u8bc1\u589e\u6b96\u901f\u5ea6\uff08\u786e\u4fdd\uff5e24 \u5c0f\u65f6\u500d\u589e\uff09\u53ca TNF alpha \u4fc3\u751f\u957f\u6548\u679c\uff08\u589e\u6b96\u52a0\u901f\u226520%\uff09\uff0c\u786e\u4fdd\u5b9e\u9a8c\u91cd\u590d\u6027\u53ca\u75ab\u82d7\u9002\u914d\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u80cc\u666f\u7b80\u4ecb<\/b>\uff1a\u4eba\u6b63\u5e38\u80da\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u7ecf\u5178\u7ec6\u80de\u7cfb\uff0c\u6e90\u81ea\u598a\u5a20 3 \u4e2a\u6708\u5973\u6027\u80da\u80ce\u80ba\u7ec4\u7ec7\uff0c\u9996\u682a\u7528\u4e8e\u4eba\u7c7b\u75ab\u82d7\u5236\u5907\u7684\u4eba\u4e8c\u500d\u4f53\u7ec6\u80de\u7cfb\uff0cMEM \u57f9\u517b\u57fa\u5373\u53ef\u7a33\u5b9a\u751f\u957f\uff0c\u6dfb\u52a0 TNF alpha \u53ef\u4fc3\u8fdb\u589e\u6b96\uff0c\u65e0\u80bf\u7624\u7ec6\u80de\u7684\u81f4\u7624\u6027\u6216\u6fc0\u7d20\u5206\u6ccc\u529f\u80fd\uff0c\u662f\u7814\u7a76\u65e9\u671f\u80da\u80ce\u80ba\u53d1\u80b2\u3001\u75ab\u82d7\u751f\u4ea7\u5de5\u827a\u3001TNF alpha \u4fe1\u53f7\u901a\u8def\u53ca\u836f\u7269\u6bd2\u6027\u8bc4\u4ef7\u7684\u7406\u60f3\u6a21\u578b\uff1b\u53ef\u4e0e MRC-5 \u5bf9\u6bd4\u63a2\u7d22\u6027\u522b \/ \u80ce\u9f84\u5bf9\u80da\u80ba\u6210\u7ea4\u7ef4\u7ec6\u80de\u589e\u6b96\u53ca\u529f\u80fd\u7684\u5f71\u54cd\uff08\u5982\u75ab\u82d7\u751f\u4ea7\u6548\u7387\u5dee\u5f02\uff09\uff0c\u6216\u4e0e JEG-3 \u6784\u5efa \u201c\u6b63\u5e38\u80da\u80ce &#8211; \u80bf\u7624\u201d \u7ec6\u80de\u914d\u5bf9\u6a21\u578b\uff0c\u8bc4\u4f30\u75ab\u82d7\u4f50\u5242\u5bf9\u6b63\u5e38\u7ec6\u80de\u7684\u5b89\u5168\u6027\u53ca\u5bf9\u80bf\u7624\u7ec6\u80de\u7684\u6f5c\u5728\u5f71\u54cd\uff0c\u4e5f\u53ef\u4f5c\u4e3a\u65e9\u671f\u80da\u80ce\u7ec6\u80de\u4e0e MRC-5\uff08\u665a\u671f\u80ce\u513f\u7ec6\u80de\uff09\u5f62\u6210 \u201c\u65e9\u671f &#8211; \u665a\u671f\u201d \u80da\u80ce\u53d1\u80b2\u9636\u6bb5\u5bf9\u6bd4\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u89c4\u683c<\/b>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226585%\uff08\u9ad8\u4e8e MRC-5\u3001JEG-3 \u7684 80%\uff0c\u9002\u914d\u77ed\u500d\u589e\u7ec6\u80de\u9ad8\u6d3b\u6027\u7279\u5f81\uff1b\u65e9\u671f\u80da\u80ce\u7ec6\u80de\u590d\u82cf\u540e\u9002\u5e94\u5feb\uff0c48 \u5c0f\u65f6\u5185\u589e\u6b96\u7387\u8fbe 100%\uff0c\u9700\u540c\u6b65\u9a8c\u8bc1 TNF alpha \u4fc3\u751f\u957f\u529f\u80fd\uff08\u6dfb\u52a0\u540e 24 \u5c0f\u65f6\u7ec6\u80de\u6570\u7ffb\u500d\uff09\uff0c\u786e\u4fdd\u6838\u5fc3\u7279\u6027\u672a\u4e22\u5931\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u652f\u539f\u4f53\u68c0\u6d4b<\/b>\uff1a\u65e0\uff08\u907f\u514d\u6c61\u67d3\u5e72\u6270\u6b63\u5e38\u6210\u7ea4\u7ef4\u7ec6\u80de\u589e\u6b96\u3001\u75ab\u82d7\u5236\u5907\u5de5\u827a\u53ca TNF alpha \u8c03\u63a7\u5b9e\u9a8c\uff0c\u4fdd\u969c \u201c\u6b63\u5e38 &#8211; \u80bf\u7624\u201d\u201c\u65e9\u671f &#8211; \u665a\u671f\u80da\u80ce\u201d \u5e73\u884c\u5b9e\u9a8c\u53ef\u9760\u6027\uff0c\u4e0e MRC-5\u3001JEG-3 \u7684\u68c0\u6d4b\u6807\u51c6\u7edf\u4e00\uff0c\u65e0\u80bf\u7624\u7ec6\u80de\u529f\u80fd\u76f8\u5173\u7684\u6c61\u67d3\u53e0\u52a0\u98ce\u9669\uff0c\u9002\u914d\u75ab\u82d7\u751f\u4ea7\u7ea7\u522b\u7684\u65e0\u83cc\u8981\u6c42\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u4fdd\u85cf\u673a\u6784<\/b>\uff1aATCC; CCL-75\u3001CRL-7728\uff0cECACC; 90020107\uff08\u56fd\u9645\u6743\u5a01\u673a\u6784\u4fdd\u85cf\uff0c\u75ab\u82d7\u5236\u5907\u7528\u7ec6\u80de\u7279\u6027\u53ca\u4e8c\u500d\u4f53\u6838\u578b\u7ecf\u591a\u673a\u6784\u9a8c\u8bc1\uff0c\u4e0e MRC-5 \u7684 ATCC; CCL-171\u3001JEG-3 \u7684\u591a\u56fd\u9645\u4fdd\u85cf\u5f62\u6210\u4e92\u8865\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u75ab\u82d7\u7814\u53d1\u5b9e\u9a8c\u7684\u7ec6\u80de\u8eab\u4efd\u786e\u8ba4\uff0c\u786e\u4fdd\u7ec6\u80de\u6765\u6e90\u7b26\u5408 GMP \u76f8\u5173\u89c4\u8303\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u57fa<\/b>\uff1aMEM+10% FBS+1% \u53cc\u6297\uff08\u6838\u5fc3\u914d\u65b9\u4e0e MRC-5 \u7684 \u201cMEM+10% FBS+PS\u201d\u3001JEG-3 \u7684 \u201c90% MEM+10% FBS+PS\u201d \u76f8\u8fd1\uff0c\u53cc\u6297\u6210\u5206\uff08\u9752\u9709\u7d20 + \u94fe\u9709\u7d20\uff09\u6d53\u5ea6\u660e\u786e\uff0c\u53ef\u4e0e\u4e24\u8005\u5171\u7528\u57fa\u7840\u57f9\u517b\u57fa\uff08\u9700\u7edf\u4e00\u53cc\u6297\u6d53\u5ea6\uff09\u964d\u4f4e\u6210\u672c\uff1b\u8840\u6e05\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u80ce\u725b\u8840\u6e05\uff08\u22645EU\/mL\uff0c\u9ad8\u4e8e MRC-5 \u7684 10EU\/mL \u6807\u51c6\uff09\uff0c\u9002\u914d\u75ab\u82d7\u751f\u4ea7\u7684\u4f4e\u6c61\u67d3\u98ce\u9669\u8981\u6c42\uff1b\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0cMEM \u57f9\u517b\u57fa\u907f\u514d\u53cd\u590d\u52a0\u70ed\uff0c\u9632\u6b62\u7834\u574f TNF alpha \u54cd\u5e94\u76f8\u5173\u4fe1\u53f7\u5206\u5b50\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u6761\u4ef6<\/b>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff08\u4e0e MRC-5\u3001JEG-3 \u57f9\u517b\u73af\u5883\u53c2\u6570\u4e00\u81f4\uff0c\u65e0\u9700\u989d\u5916\u8c03\u6574\uff1b\u65e9\u671f\u80da\u80ce\u7ec6\u80de\u5bf9\u6e29\u5ea6\u6ce2\u52a8\u654f\u611f\uff0c\u57f9\u517b\u7bb1\u63a7\u6e29\u6ce2\u52a8\u9700\u2264\u00b10.2\u2103\uff0c\u907f\u514d\u5f71\u54cd\u77ed\u500d\u589e\u7279\u6027\uff1b\u56e0\u589e\u6b96\u5feb\u9700\u6bcf 24 \u5c0f\u65f6\u66f4\u6362 1 \u6b21\u57f9\u517b\u57fa\uff0c\u6dfb\u52a0 TNF alpha \u65f6\u9700\u540c\u6b65\u8c03\u6574 pH \u81f3 7.2-7.4\uff0c\u786e\u4fdd\u7ec6\u80de\u56e0\u5b50\u6d3b\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u51bb\u5b58\u6761\u4ef6<\/b>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff083\u00d710\u2076cells\/mL\uff0c\u9ad8\u4e8e MRC-5 \u7684 2\u00d710\u2076cells\/mL\uff1b4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u50a8\u5b58\uff1b\u51bb\u5b58\u524d\u9700\u5728\u542b TNF alpha \u7684\u57f9\u517b\u57fa\u4e2d\u57f9\u517b 12 \u5c0f\u65f6\uff08\u589e\u5f3a\u7ec6\u80de\u6297\u51bb\u6027\uff09\uff0c\u6dfb\u52a0 10% FBS+5% DMSO\uff08\u5e38\u89c4\u51bb\u5b58\u6db2 DMSO \u6d53\u5ea6 5%\uff0c\u4f4e\u4e8e JEG-3 \u7684 10%\uff09\uff0c\u964d\u4f4e\u65e9\u671f\u80da\u80ce\u7ec6\u80de\u51bb\u5b58\u635f\u4f24\uff0c\u590d\u82cf\u5b58\u6d3b\u7387\u6bd4 MRC-5 \u9ad8 5%-10%\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>STR \u4f4d\u70b9\u4fe1\u606f<\/b>\uff1aAmelogenin\uff1aX\uff08\u786e\u8ba4\u5973\u6027\u6838\u578b\uff0c\u4e0e\u6027\u522b\u6807\u6ce8\u4e00\u81f4\uff0c\u533a\u522b\u4e8e MRC-5 \u7684 \u201cX,Y\u201d\u3001JEG-3 \u7684 \u201cX,Y\u201d\uff09\uff1bCSF1PO\uff1a10\uff0c12\uff1bD13S317\uff1a11\uff1bD16S539\uff1a11\uff0c12\uff1bD18S51\uff1a16\uff0c18\uff1bD19S433\uff1a13\uff0c16.2\uff1bD21S11\uff1a30\uff0c30.2\uff1bD2S1338\uff1a19\uff0c25\uff1bD3S1358\uff1a16\uff0c17\uff1bD5S818\uff1a10\uff1bD7S820\uff1a9\uff0c11\uff1bD8S1179\uff1a14\uff1bFGA\uff1a22\uff0c24\uff1bTH01\uff1a8\uff0c9.3\uff1bTPOX\uff1a8\uff1bvWA\uff1a19\uff0c20\uff08\u542b\u5973\u6027\u65e9\u671f\u80da\u80ce\u80ba\u7ec6\u80de\u7279\u5f81\u6027 STR \u4f4d\u70b9\uff0c\u53ef\u4e0e MRC-5\u3001JEG-3 \u56fe\u8c31\u5bf9\u6bd4\u786e\u8ba4\u8eab\u4efd\uff0c\u9a8c\u8bc1\u4e8c\u500d\u4f53\u6838\u578b\u7a33\u5b9a\u6027\u53ca\u6027\u522b\u7279\u5f02\u6027\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u8d27\u671f<\/b>\uff1a2 \u5468\u5de6\u53f3\uff08\u957f\u4e8e MRC-5\u3001JEG-3 \u7684 1 \u5468\uff0c\u56e0\u65e9\u671f\u80da\u80ce\u7ec6\u80de\u57f9\u517b\u53ca\u75ab\u82d7\u9002\u914d\u6027\u9a8c\u8bc1\u6d41\u7a0b\u66f4\u4e25\u683c\uff0c\u9700\u63d0\u524d\u5907\u8d27\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u8fd0\u8f93\u65b9\u5f0f<\/b>\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff09\uff1b\u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09\uff0c\u987a\u4e30\u5feb\u9012<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u4f9b\u5e94\u9650\u5236<\/b>\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e MRC-5\u3001JEG-3 \u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u867d\u4e3a\u75ab\u82d7\u5236\u5907\u7528\u7ec6\u80de\uff0c\u4f46\u4e0d\u53ef\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u6216\u4eba\u4f53\u5e72\u9884\u5b9e\u9a8c\uff0c\u4e25\u7981\u7528\u4e8e\u751f\u6b96\u76f8\u5173\u7814\u7a76\u6216\u672a\u7ecf\u5ba1\u6279\u7684\u75ab\u82d7\u751f\u4ea7\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7279\u522b\u8bf4\u660e<\/b>\uff1a\u2460 \u529f\u80fd\u9a8c\u8bc1\uff1a\u9996\u6b21\u57f9\u517b\u901a\u8fc7 STR \u68c0\u6d4b\u786e\u8ba4\u5973\u6027\u6838\u578b\uff08Amelogenin\uff1aX\uff09\uff0c\u6dfb\u52a0 TNF alpha\uff0810ng\/mL\uff09\u9a8c\u8bc1\u589e\u6b96\u52a0\u901f\u6548\u679c\uff0824 \u5c0f\u65f6\u7ec6\u80de\u6570\u2265\u521d\u59cb 2 \u500d\uff09\uff0c\u955c\u68c0\u89c2\u5bdf\u6210\u7ea4\u7ef4\u7ec6\u80de\u5f62\u6001\u5360\u6bd4\uff08\u226595%\uff09\uff0c\u786e\u4fdd\u7ec6\u80de\u8eab\u4efd\u53ca\u6838\u5fc3\u7279\u6027\u7a33\u5b9a\uff1b\u2461 \u57f9\u517b\u6ce8\u610f\uff1aMEM \u57f9\u517b\u57fa\u9700\u9009\u62e9\u9ad8\u7cd6\u578b\uff08\u8461\u8404\u7cd6 4.5g\/L\uff09\uff0c\u53cc\u6297\u6d53\u5ea6\u9700\u4e25\u683c\u63a7\u5236\uff08\u9752\u9709\u7d20 100U\/mL + \u94fe\u9709\u7d20 100\u03bcg\/mL\uff09\uff0c\u907f\u514d\u5f71\u54cd\u75ab\u82d7\u76f8\u5173\u86cb\u767d\u8868\u8fbe\uff1b\u4f20\u4ee3\u65f6\u9700\u4f7f\u7528\u65e0\u9176\u5439\u6253\u6db2\u8f85\u52a9\u5206\u6563\u805a\u56e2\u7ec6\u80de\uff0c\u51cf\u5c11\u5bf9\u7ec6\u80de\u8868\u9762\u53d7\u4f53\u7684\u635f\u4f24\uff08\u4fdd\u969c TNF alpha \u54cd\u5e94\u6027\uff09\uff1b\u2462 \u5b9e\u9a8c\u9002\u914d\uff1a\u9002\u5408\u65e9\u671f\u80da\u80ce\u80ba\u53d1\u80b2\u673a\u5236\u7814\u7a76\u3001\u75ab\u82d7\u751f\u4ea7\u5de5\u827a\u4f18\u5316\uff08\u5982\u810a\u9ad3\u7070\u8d28\u708e\u75ab\u82d7\u3001\u9ebb\u75b9\u75ab\u82d7\uff09\u3001TNF alpha \u4fe1\u53f7\u901a\u8def\u8c03\u63a7\u5b9e\u9a8c\u3001\u836f\u7269\u6bd2\u6027\u8bc4\u4ef7\uff08\u4f5c\u4e3a\u75ab\u82d7\u4f50\u5242\u5b89\u5168\u6027\u5bf9\u7167\uff09\uff1b\u53ef\u4e0e MRC-5 \u540c\u6b65\u5f00\u5c55 \u201c\u6027\u522b \/ \u80ce\u9f84\u5bf9\u80da\u80ba\u7ec6\u80de\u589e\u6b96\u53ca\u75ab\u82d7\u751f\u4ea7\u6548\u7387\u7684\u5f71\u54cd\u201d \u5bf9\u6bd4\uff0c\u6216\u4e0e JEG-3 \u63a2\u7d22\u75ab\u82d7\u4f50\u5242\u5bf9\u6b63\u5e38\u7ec6\u80de\u4e0e\u80bf\u7624\u7ec6\u80de\u7684\u5dee\u5f02\u5316\u4f5c\u7528\uff0c\u4e5f\u53ef\u4f5c\u4e3a\u65e9\u671f\u80da\u80ce\u6210\u7ea4\u7ef4\u7ec6\u80de\u6a21\u578b\u7528\u4e8e\u7ec6\u80de\u5916\u57fa\u8d28\u5408\u6210\uff08\u5982\u80f6\u539f I\/III 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