{"id":297,"date":"2025-08-25T21:24:30","date_gmt":"2025-08-25T13:24:30","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=297"},"modified":"2025-08-25T21:24:30","modified_gmt":"2025-08-25T13:24:30","slug":"293t%e4%ba%ba%e8%83%9a%e8%82%be%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/293t%e4%ba%ba%e8%83%9a%e8%82%be%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"293T\u4eba\u80da\u80be\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u540d\u79f0<\/b>\uff1a293T \u4eba\u80da\u80be\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09<\/li>\n<\/ul>\n<div data-zone-id=\"0\" data-line-index=\"2\" data-line=\"true\">\u6838\u5fc3\u7279\u8272\uff1a\u4eba\u80da\u80be\u6765\u6e90 + SV40 T &#8211; 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\u80ba\uff09\u5bf9\u7ec6\u80de\u5f62\u6001\u53ca\u529f\u80fd\u7684\u5f71\u54cd\uff0c\u6216\u4e0e MRC-5 \u6784\u5efa \u201c\u5de5\u5177\u7ec6\u80de &#8211; \u6b63\u5e38\u7ec6\u80de\u201d \u914d\u5bf9\u6a21\u578b\uff0c\u8bc4\u4f30\u57fa\u56e0\u8f6c\u67d3\u5bf9\u4e0d\u540c\u80da\u80ce\u7ec6\u80de\u7684\u6bd2\u6027\u5dee\u5f02\uff0c\u4e5f\u53ef\u4f5c\u4e3a\u9ad8\u8f6c\u67d3\u6a21\u578b\u4e0e\u4f4e\u8f6c\u67d3\u6548\u7387\u7ec6\u80de\uff08\u5982\u539f\u4ee3\u7ec6\u80de\uff09\u5f62\u6210\u8f6c\u67d3\u6548\u7387\u5bf9\u6bd4\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u89c4\u683c<\/b>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226585%\uff08\u9ad8\u4e8e WI-38\u3001MRC-5 \u7684 80%\uff0c\u9002\u914d\u5de5\u5177\u7ec6\u80de\u9ad8\u6d3b\u6027\u9700\u6c42\uff1b\u590d\u82cf\u540e\u56e0\u8d34\u58c1\u4e0d\u7262\uff0c\u9700\u5728\u57f9\u517b\u74f6\u9884\u94fa 0.1% \u660e\u80f6\uff08\u53ef\u9009\uff09\u63d0\u5347\u8d34\u58c1\u7387\uff0c24 \u5c0f\u65f6\u5185\u8d34\u58c1\u7387\u5e94\u226590%\uff0c\u540c\u6b65\u9a8c\u8bc1\u8f6c\u67d3\u6548\u7387\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u652f\u539f\u4f53\u68c0\u6d4b<\/b>\uff1a\u65e0\uff08\u907f\u514d\u6c61\u67d3\u5e72\u6270\u57fa\u56e0\u8f6c\u67d3\u3001\u75c5\u6bd2\u5305\u88c5\u53ca\u86cb\u767d\u8868\u8fbe\u5b9e\u9a8c\uff0c\u4fdd\u969c \u201c\u5de5\u5177\u7ec6\u80de &#8211; \u6b63\u5e38\u7ec6\u80de\u201d \u5e73\u884c\u5b9e\u9a8c\u53ef\u9760\u6027\uff0c\u4e0e WI-38\u3001MRC-5 \u7684\u68c0\u6d4b\u6807\u51c6\u7edf\u4e00\uff0c\u5de5\u5177\u7ec6\u80de\u5bf9\u6c61\u67d3\u66f4\u654f\u611f\uff08\u5f71\u54cd\u8f6c\u67d3\u6548\u7387\uff09\uff0c\u9700\u66f4\u9891\u7e41\u68c0\u6d4b\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u4fdd\u85cf\u673a\u6784<\/b>\uff1aATCC; CRL-3216\uff0cDSMZ; ACC-635\uff1b\u4e2d\u56fd\u5178\u578b\u57f9\u517b\u7269\u4fdd\u85cf\u4e2d\u5fc3\u7ec6\u80de\u5e93\uff08\u56fd\u9645 + \u56fd\u5185\u673a\u6784\u4fdd\u85cf\uff0c\u57fa\u56e0\u4fee\u9970\u7279\u6027\u53ca\u9ad8\u8f6c\u67d3\u6548\u7387\u7ecf\u591a\u673a\u6784\u9a8c\u8bc1\uff0c\u4e0e WI-38 \u7684 ATCC; CCL-75\u3001MRC-5 \u7684 ATCC; CCL-171 \u5f62\u6210\u4e92\u8865\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u57fa\u56e0\u5de5\u7a0b\u5b9e\u9a8c\u7684\u7ec6\u80de\u8eab\u4efd\u786e\u8ba4\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u57fa<\/b>\uff1a90% DMEM+10% FBS+PS\uff08\u6838\u5fc3\u914d\u65b9\u5dee\u5f02\uff0c\u57fa\u7840\u57f9\u517b\u57fa\u4e3a DMEM\uff08\u9ad8\u7cd6\u578b\uff0c\u9002\u914d\u5de5\u5177\u7ec6\u80de\u80fd\u91cf\u9700\u6c42\uff09\uff0c\u533a\u522b\u4e8e WI-38\u3001MRC-5 \u7684 \u201cMEM \u57f9\u517b\u57fa\u201d\uff0c\u4e0d\u53ef\u6df7\u7528\uff1b\u8840\u6e05\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u80ce\u725b\u8840\u6e05\uff08\u226410EU\/mL\uff09\uff0c\u9700\u786e\u4fdd\u65e0\u652f\u539f\u4f53\u53ca\u75c5\u6bd2\u6c61\u67d3\uff08\u907f\u514d\u5f71\u54cd\u75c5\u6bd2\u5305\u88c5\uff09\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0cDMEM \u57f9\u517b\u57fa\u65e0\u9700\u907f\u5149\uff0c\u64cd\u4f5c\u4fbf\u6377\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u6761\u4ef6<\/b>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff08\u4e0e WI-38\u3001MRC-5 \u57f9\u517b\u73af\u5883\u53c2\u6570\u4e00\u81f4\uff0c\u65e0\u9700\u989d\u5916\u8c03\u6574\uff1b\u56e0\u8d34\u58c1\u4e0d\u7262\uff0c\u57f9\u517b\u7bb1\u9700\u907f\u514d\u5267\u70c8\u9707\u52a8\uff0c\u6e7f\u5ea6\u7a33\u5b9a\u5728 75%\u00b15%\uff08\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4\u7ec6\u80de\u8131\u843d\uff09\uff1b\u77ed\u500d\u589e\u7279\u6027\u4f7f\u8425\u517b\u6d88\u8017\u5feb\uff0c\u9700\u6bcf 24 \u5c0f\u65f6\u66f4\u6362 1 \u6b21\u57f9\u517b\u57fa\uff0c\u907f\u514d\u4ee3\u8c22\u5e9f\u7269\u5f71\u54cd\u8f6c\u67d3\u6548\u7387\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u51bb\u5b58\u6761\u4ef6<\/b>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff083\u00d710\u2076cells\/mL\uff0c\u9ad8\u4e8e WI-38\u3001MRC-5 \u7684 2\u00d710\u2076cells\/mL\uff1b4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u50a8\u5b58\uff1b\u51bb\u5b58\u524d\u9700\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff08\u8f6c\u67d3\u6548\u7387\u6700\u9ad8\u671f\uff09\uff0c\u6dfb\u52a0 10% FBS \u53ef\u63d0\u5347\u590d\u82cf\u540e\u8d34\u58c1\u80fd\u529b\uff0c\u907f\u514d\u51bb\u5b58\u5e73\u53f0\u671f\u7ec6\u80de\u5bfc\u81f4\u5b58\u6d3b\u7387\u4e0b\u964d\uff1b\u590d\u82cf\u65f6\u5feb\u901f\u6c34\u6d74\uff0837\u2103\uff0c1 \u5206\u949f\u5185\uff09\u6eb6\u89e3\uff0c\u79bb\u5fc3\u540e\u91cd\u60ac\u94fa\u74f6\uff08\u51cf\u5c11 DMSO \u5bf9\u8d34\u58c1\u7684\u5f71\u54cd\uff09\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>STR \u4f4d\u70b9\u4fe1\u606f<\/b>\uff1aAmelogenin:X\uff08\u63d0\u793a\u5973\u6027\u6838\u578b\u6216\u7537\u6027\u4f4d\u70b9\u68c0\u6d4b\u9057\u6f0f\uff0c\u4e0e WI-38 \u7684 \u201cX\u201d\uff08\u5973\u6027\uff09\u3001MRC-5 \u7684 \u201cX,Y\u201d\uff08\u7537\u6027\uff09\u5f62\u6210\u6027\u522b\u5173\u8054\uff09\uff1bCSF1PO:11,12\uff1bD13S317:12,14\uff1bD16S539:9,13\uff1bD18S51:17,18\uff1bD21S11:28,30.2\uff1bD3S1358:15,16,17\uff1bD5S818:8,9\uff1bD7S820:11\uff1bD8S1179:12,14\uff1bFGA:23\uff1bPentaD:9,10\uff1bPentaE:7,15\uff1bTH01:7,9.3\uff1bTPOX:11\uff1bVWA:16,19\uff08\u542b\u80da\u80be\u7ec6\u80de\u7279\u5f81\u6027 STR \u4f4d\u70b9\uff0c\u53ef\u4e0e WI-38\u3001MRC-5 \u56fe\u8c31\u5bf9\u6bd4\u786e\u8ba4\u8eab\u4efd\uff0c\u9a8c\u8bc1 SV40 T &#8211; \u6297\u539f\u4fee\u9970\u672a\u5bfc\u81f4\u6838\u5fc3 STR \u4f4d\u70b9\u6539\u53d8\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u8d27\u671f<\/b>\uff1a\u73b0\u8d27\uff0c1 \u5468\u5de6\u53f3\uff08\u77ed\u4e8e WI-38 \u7684 2 \u5468\uff0c\u5de5\u5177\u7ec6\u80de\u5e38\u89c4\u5907\u8d27\u5145\u8db3\uff0c\u9002\u914d\u5b9e\u9a8c\u7d27\u6025\u9700\u6c42\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u8fd0\u8f93\u65b9\u5f0f<\/b>\uff1a\u590d\u82cf\u53d1\u8d27\uff08T25 \u74f6\u514d\u8fd0\u8f93\u8d39\u7528\uff0c\u9700\u7279\u522b\u6807\u6ce8 \u201c\u8d34\u58c1\u4e0d\u7262\uff0c\u907f\u514d\u5267\u70c8\u9707\u52a8\u201d\uff09\uff1b\u51bb\u5b58\u53d1\u8d27\uff08\u9700\u52a0\u5e72\u51b0\u8fd0\u8f93\u8d39\u7528\uff09\uff0c\u987a\u4e30\u5feb\u9012<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u4f9b\u5e94\u9650\u5236<\/b>\uff1a\u4ec5\u9650\u4e8e\u79d1\u5b66\u7814\u7a76\uff0c\u7edd\u4e0d\u53ef\u4f5c\u4e3a\u52a8\u7269\u6216\u4eba\u7c7b\u75be\u75c5\u7684\u6cbb\u7597\u4ea7\u54c1\u4f7f\u7528\uff08\u4e0e WI-38\u3001MRC-5 \u9650\u5236\u6761\u6b3e\u4e00\u81f4\uff0c\u867d\u4e3a\u57fa\u56e0\u5de5\u7a0b\u5de5\u5177\u7ec6\u80de\uff0c\u4f46\u4e0d\u53ef\u7528\u4e8e\u4e34\u5e8a\u6cbb\u7597\u6216\u4eba\u4f53\u5e72\u9884\u5b9e\u9a8c\uff0c\u4e25\u7981\u7528\u4e8e\u672a\u7ecf\u5ba1\u6279\u7684\u57fa\u56e0\u6cbb\u7597\u76f8\u5173\u7814\u7a76\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7279\u522b\u8bf4\u660e<\/b>\uff1a\u2460 \u8f6c\u67d3\u9a8c\u8bc1\uff1a\u9996\u6b21\u57f9\u517b\u901a\u8fc7 Lipofectamine \u7b49\u8bd5\u5242\u8f6c\u67d3 EGFP \u8367\u5149\u8d28\u7c92\uff0c24-48 \u5c0f\u65f6\u540e\u8367\u5149\u663e\u5fae\u955c\u89c2\u5bdf\u8f6c\u67d3\u6548\u7387\uff08\u226550%\uff09\uff0c\u786e\u8ba4\u5de5\u5177\u7ec6\u80de\u5c5e\u6027\uff1b\u2461 \u64cd\u4f5c\u6ce8\u610f\uff1a\u56e0\u8d34\u58c1\u4e0d\u7262\uff0c\u6362\u6db2\u65f6\u9700\u6cbf\u57f9\u517b\u74f6\u58c1\u7f13\u6162\u52a0\u5165\u57f9\u517b\u57fa\uff08\u907f\u514d\u76f4\u51b2\u7ec6\u80de\uff09\uff0c\u8fd0\u8f93\u540e\u82e5\u53d1\u73b0\u7ec6\u80de\u8131\u843d\uff0c\u53ef\u6536\u96c6\u4e0a\u6e05\u79bb\u5fc3\uff081000rpm\uff0c3 \u5206\u949f\uff09\uff0c\u7528\u65b0\u9c9c\u57f9\u517b\u57fa\u91cd\u60ac\u540e\u91cd\u65b0\u94fa\u74f6\uff08\u6dfb\u52a0\u660e\u80f6\u53ef\u63d0\u5347\u8d34\u58c1\u7387\uff09\uff1b\u4f20\u4ee3\u65f6\u907f\u514d\u8fc7\u5ea6\u5439\u6253\uff08\u9632\u6b62\u7ec6\u80de\u7834\u788e\u5f71\u54cd\u540e\u7eed\u5b9e\u9a8c\uff09\uff1b\u2462 \u5b9e\u9a8c\u9002\u914d\uff1a\u9002\u5408\u57fa\u56e0\u529f\u80fd\u9a8c\u8bc1\uff08\u5982\u8fc7\u8868\u8fbe \/ \u6572\u964d\u7279\u5b9a\u57fa\u56e0\uff09\u3001\u75c5\u6bd2\u5305\u88c5\uff08\u6162\u75c5\u6bd2\u3001\u817a\u75c5\u6bd2\uff09\u3001\u91cd\u7ec4\u86cb\u767d\u8868\u8fbe\uff08\u5982\u6297\u4f53\u3001\u7ec6\u80de\u56e0\u5b50\uff09\u3001\u836f\u7269\u7b5b\u9009\uff08\u4f5c\u4e3a\u8f6c\u67d3\u6a21\u578b\u8bc4\u4f30\u836f\u7269\u5bf9\u9776\u57fa\u56e0\u7684\u5f71\u54cd\uff09\uff1b\u53ef\u4e0e WI-38 \u540c\u6b65\u5f00\u5c55 \u201c\u80be &#8211; 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