{"id":303,"date":"2025-08-25T21:57:24","date_gmt":"2025-08-25T13:57:24","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=303"},"modified":"2025-08-25T21:57:24","modified_gmt":"2025-08-25T13:57:24","slug":"nci-h358%e4%ba%ba%e9%9d%9e%e5%b0%8f%e7%bb%86%e8%83%9e%e8%82%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/nci-h358%e4%ba%ba%e9%9d%9e%e5%b0%8f%e7%bb%86%e8%83%9e%e8%82%ba%e7%99%8c%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"NCI-H358\u4eba\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<div data-zone-id=\"0\" data-line-index=\"0\" data-line=\"true\">\n<h2 class=\"heading-h2\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<\/div>\n<ul>\n<li 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data-list=\"bullet1\"><b>\u80cc\u666f\u7b80\u4ecb<\/b>\uff1a1981 \u5e74\u4ece\u5316\u7597\u524d\u7537\u6027\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\uff08\u7ec6\u652f\u6c14\u7ba1\u80ba\u6ce1\u764c\uff09\u60a3\u8005\u80bf\u7624\u7ec4\u7ec7\u4e2d\u5efa\u682a\uff0c\u8d85\u5fae\u7ed3\u6784\u542b Clara \u7ec6\u80de\u7279\u5f81\u7ed3\u6784\uff08\u80ba\u6ce1\u4e0a\u76ae\u7ec6\u80de\u6807\u5fd7\u7269\uff09\uff0c\u8868\u8fbe\u80ba\u8868\u9762\u86cb\u767d SP-A\uff08RNA \u53ca\u86cb\u767d\u6c34\u5e73\uff09\uff0c\u4e0d\u8868\u8fbe SP-B\/SP-C\uff1b\u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u6548\u7387 0.83%\uff08\u4f53\u5916\u6076\u6027\u7279\u5f81\uff09\uff0c\u88f8\u9f20\u63a5\u79cd\u53ef\u6210\u7624\uff1b\u65e0 NCI-H460 \u7684\u80f8\u8154\u8f6c\u79fb\u5c5e\u6027\u6216 MRC-5 \u7684\u6b63\u5e38\u7ec6\u80de\u529f\u80fd\uff0c\u662f\u7814\u7a76\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\uff08\u80ba\u6ce1\u4e9a\u578b\uff09\u3001KRAS \u7a81\u53d8\u673a\u5236\u3001\u80ba\u6ce1\u4e0a\u76ae\u529f\u80fd\u5f02\u5e38\u53ca\u80ba\u764c\u4e9a\u578b\u5dee\u5f02\u7684\u7406\u60f3\u6a21\u578b\uff1b\u53ef\u4e0e NCI-H460 \u5bf9\u6bd4\u63a2\u7d22\u80ba\u764c\u4e9a\u578b\uff08\u80ba\u6ce1\u764c &#8211; \u5927\u7ec6\u80de\u764c\uff09\u7684\u589e\u6b96\u3001\u81f4\u7624\u53ca\u836f\u7269\u654f\u611f\u6027\u5dee\u5f02\uff0c\u6216\u4e0e MRC-5 \u6784\u5efa \u201c\u80bf\u7624 &#8211; \u6b63\u5e38\u201d \u80ba\u7ec6\u80de\u914d\u5bf9\u6a21\u578b\uff0c\u8bc4\u4f30 KRAS \u9776\u5411\u836f\u7269\u5bf9\u6b63\u5e38\u80ba\u7ec6\u80de\u7684\u6bd2\u6027\u3002<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>STR \u4f4d\u70b9\u4fe1\u606f<\/b>\uff1aAmelogenin:X,Y\uff08\u786e\u8ba4\u7537\u6027\u6838\u578b\uff0c\u4e0e\u6027\u522b\u6807\u6ce8\u4e00\u81f4\uff0c\u533a\u522b\u4e8e NCI-H460 \u7684 \u201cX,Y\u201d\u3001MRC-5 \u7684 \u201cX,Y\u201d\uff0c\u4e09\u8005\u6027\u522b\u4e00\u81f4\u53ef\u6392\u9664\u6027\u522b\u5bf9\u5b9e\u9a8c\u7684\u5e72\u6270\uff09\uff1bCSF1PO:11\uff0c12\uff1bD13S317:8\uff0c12\uff1bD16S539:12\uff0c13\uff1bD18S51:14\uff1bD19S433:13\uff0c14\uff1bD21S11:28\uff0c30\uff1bD2S1338:17\uff0c23\uff1bD3S1358:14\uff0c18\uff1bD5S818:10,12\uff1bD7S820:10\uff0c11\uff1bD8S1179:13\uff0c14\uff1bFGA:20,21\uff1bTH01:6\uff1bTPOX:8\uff0c9\uff1bvWA:17\uff08\u542b\u7537\u6027\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u7279\u5f81\u6027 STR \u4f4d\u70b9\uff0c\u53ef\u4e0e NCI-H460\u3001MRC-5 \u56fe\u8c31\u5bf9\u6bd4\u786e\u8ba4\u8eab\u4efd\uff0c\u9a8c\u8bc1\u65e0\u5176\u4ed6\u7ec4\u7ec7\u6765\u6e90\u7ec6\u80de\u6c61\u67d3\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u7ec6\u80de\u89c4\u683c<\/b>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226585%\uff08\u4e0e NCI-H460\u3001MRC-5 \u7684\u5b58\u6d3b\u7387\u6807\u51c6\u4e00\u81f4\uff1b\u7eaf\u8d34\u58c1\u7ec6\u80de\u590d\u82cf\u9002\u5e94\u5feb\uff0c24 \u5c0f\u65f6\u5185\u8d34\u58c1\u7387\u226590%\uff0c\u9700\u540c\u6b65\u9a8c\u8bc1 SP-A \u8868\u8fbe\uff08\u514d\u75ab\u8367\u5149\u9633\u6027\u7387\u226585%\uff09\u53ca KRAS \u7a81\u53d8\u72b6\u6001\uff08\u6d4b\u5e8f\u786e\u8ba4\u6742\u5408\u5b50\uff09\uff0c\u786e\u4fdd\u7ec6\u80de\u5c5e\u6027\u7a33\u5b9a\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u652f\u539f\u4f53\u68c0\u6d4b<\/b>\uff1a\u65e0\uff08\u660e\u786e\u6392\u9664\u5fae\u751f\u7269\u6c61\u67d3\uff0c\u4e0e NCI-H460 \u7684 \u201c\u65e0\u201d\u3001MRC-5 \u7684 \u201c\u65e0\u201d \u6807\u51c6\u4e00\u81f4\uff1b\u56e0\u6d89\u53ca KRAS \u9776\u5411\u5b9e\u9a8c\uff0c\u5fae\u751f\u7269\u68c0\u6d4b\u9891\u6b21\u9700\u9ad8\u4e8e\u666e\u901a\u7ec6\u80de\uff0c\u5efa\u8bae\u6bcf 2 \u4ee3\u901a\u8fc7 PCR \u6cd5\u590d\u6d4b\uff0c\u907f\u514d\u6c61\u67d3\u5f71\u54cd\u7a81\u53d8\u68c0\u6d4b\u53ca\u836f\u7269\u7b5b\u9009\u7ed3\u679c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u4fdd\u85cf\u673a\u6784<\/b>\uff1aATCC; CRL-5807\uff0cECACC; 95111733\uff1b\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u57fa\u7840\u533b\u5b66\u7814\u7a76\u6240\u7ec6\u80de\u8d44\u6e90\u4e2d\u5fc3\uff08\u56fd\u9645 + \u56fd\u5185\u591a\u673a\u6784\u4fdd\u85cf\uff0c\u975e\u5c0f\u7ec6\u80de\u80ba\u764c\u7279\u6027\u3001STR \u9274\u5b9a\u7ed3\u679c\u3001KRAS \u7a81\u53d8\u53ca\u81f4\u7624\u6027\u7ecf\u591a\u673a\u6784\u9a8c\u8bc1\uff0c\u4e0e NCI-H460 \u7684\u56fd\u9645\u4fdd\u85cf\u3001MRC-5 \u7684\u56fd\u9645 + \u56fd\u5185\u4fdd\u85cf\u5f62\u6210\u4e92\u8865\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u9776\u5411\u6cbb\u7597\u5b9e\u9a8c\u7684\u7ec6\u80de\u6765\u6e90\u5408\u89c4\u6027\u8981\u6c42\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u57fa<\/b>\uff1aRPMI-1640+10% FBS+1% PS\uff08\u6838\u5fc3\u914d\u65b9\u4e0e NCI-H460 \u7684 \u201c90% RPMI-1640+10% FBS\u201d \u57fa\u7840\u4e00\u81f4\uff0c\u660e\u786e 1% PS\uff08\u9752\u9709\u7d20 + \u94fe\u9709\u7d20\uff09\u6d53\u5ea6\uff0c\u533a\u522b\u4e8e MRC-5 \u7684 \u201cMEM+10% FBS\u201d\uff1b\u8840\u6e05\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u80ce\u725b\u8840\u6e05\uff08\u226410EU\/mL\uff09\uff0c\u907f\u514d\u5f71\u54cd SP-A \u8868\u8fbe\u53ca KRAS \u7a81\u53d8\u76f8\u5173\u4fe1\u53f7\u901a\u8def\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0cRPMI-1640 \u57f9\u517b\u57fa\u65e0\u9700\u907f\u5149\uff0c\u53ef\u4e0e NCI-H460 \u5171\u7528\u57fa\u7840\u57f9\u517b\u57fa\u964d\u4f4e\u6210\u672c\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u6761\u4ef6<\/b>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff08\u4e0e NCI-H460\u3001MRC-5 \u57f9\u517b\u73af\u5883\u53c2\u6570\u4e00\u81f4\uff0c\u65e0\u9700\u989d\u5916\u8c03\u6574\uff1b\u80ba\u6ce1\u4e0a\u76ae\u6765\u6e90\u7ec6\u80de\u5bf9\u6e7f\u5ea6\u654f\u611f\uff0c\u57f9\u517b\u7bb1\u6e7f\u5ea6\u9700\u7a33\u5b9a\u5728 75%\u00b15%\uff08\u9632\u6b62\u57f9\u517b\u57fa\u84b8\u53d1\u5bfc\u81f4 SP-A \u5408\u6210\u5f02\u5e38\uff09\uff1b\u56e0\u500d\u589e\u901f\u7387\u4e2d\u7b49\uff0c\u6bcf 48 \u5c0f\u65f6\u66f4\u6362 1 \u6b21\u57f9\u517b\u57fa\uff0c\u65e0\u9700\u50cf NCI-H460 \u90a3\u6837\u9891\u7e41\u955c\u68c0\u5bc6\u5ea6\uff0c\u64cd\u4f5c\u4fbf\u6377\u6027\u4ecb\u4e8e\u4e24\u8005\u4e4b\u95f4\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u51bb\u5b58\u6761\u4ef6<\/b>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff082\u00d710\u2076cells\/mL\uff0c\u4e0e NCI-H460\u3001MRC-5 \u7684\u5bc6\u5ea6\u4e00\u81f4\uff1b4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u50a8\u5b58\uff1b\u51bb\u5b58\u524d\u9700\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff08KRAS \u7a81\u53d8\u529f\u80fd\u7a33\u5b9a\u671f\uff09\uff0c\u6dfb\u52a0 10% FBS \u53ef\u63d0\u5347\u590d\u82cf\u540e SP-A \u8868\u8fbe\u53ca\u7a81\u53d8\u7a33\u5b9a\u6027\uff0c\u907f\u514d\u51bb\u5b58\u5e73\u53f0\u671f\u7ec6\u80de\u5bfc\u81f4\u9776\u5411\u836f\u7269\u54cd\u5e94\u5f02\u5e38\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u5206\u5b50\u4e0e\u529f\u80fd\u7279\u5f81<\/b>\uff1a\u2460 \u57fa\u56e0\u53d8\u5f02\uff1aKRAS p.Thr75Ala\uff08c.223A&gt;G\uff09\u6742\u5408\u7a81\u53d8\uff08\u53ef\u901a\u8fc7 Sanger \u6d4b\u5e8f\u6216\u6570\u5b57 PCR \u9a8c\u8bc1\uff0c\u7a81\u53d8\u9891\u7387\u226545%\uff0cKRAS \u9776\u5411\u836f\u7269\uff08\u5982 Sotorasib\uff09\u7b5b\u9009\u6838\u5fc3\u9776\u70b9\uff0c\u533a\u522b\u4e8e NCI-H460 \u7684\u65e0\u660e\u786e\u53d8\u5f02\u3001MRC-5 \u7684\u6b63\u5e38\u57fa\u56e0\uff09\uff1b\u2461 \u7ec6\u80de\u7ed3\u6784\u4e0e\u86cb\u767d\uff1a\u542b Clara \u7ec6\u80de\u7279\u5f81\u7ed3\u6784\uff08\u8d85\u5fae\u955c\u89c2\u5bdf\u53ef\u89c1\uff09\uff0c\u80ba\u8868\u9762\u86cb\u767d SP-A \u9633\u6027\uff08\u514d\u75ab\u8367\u5149 \/ Western Blot \u9a8c\u8bc1\uff0c\u9633\u6027\u7387\u226585%\uff09\uff0cSP-B\/SP-C \u9634\u6027\uff08\u6392\u9664\u5176\u4ed6\u80ba\u6ce1\u4e0a\u76ae\u7ec6\u80de\u4e9a\u578b\u6c61\u67d3\uff09\uff0c\u533a\u522b\u4e8e NCI-H460 \u7684 \u201c\u89d2\u86cb\u767d \/ \u6ce2\u5f62\u86cb\u767d\u9633\u6027\u201d\u3001MRC-5 \u7684 \u201c\u65e0\u7279\u5f02\u6027\u86cb\u767d\u201d\uff1b\u2462 \u81f4\u7624\u6027\u4e0e\u514b\u9686\u5f62\u6210\uff1a\u88f8\u9f20\u81f4\u7624\u9633\u6027\uff08\u6210\u7624\u5468\u671f 18-25 \u5929\uff0c\u957f\u4e8e NCI-H460 \u7684 14-21 \u5929\uff09\uff0c\u8f6f\u743c\u8102\u514b\u9686\u5f62\u6210\u6548\u7387 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