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data-list=\"bullet1\"><b>\u57f9\u517b\u57fa<\/b>\uff1a1640+10% FBS+1% PS\uff08\u6838\u5fc3\u914d\u65b9\u5dee\u5f02\uff0c\u57fa\u7840\u57f9\u517b\u57fa\u4e3a 1640\uff08\u9002\u914d\u60ac\u6d6e\u80bf\u7624\u7ec6\u80de\u8425\u517b\u9700\u6c42\uff09\uff0c\u533a\u522b\u4e8e SRA01\/04 \u7684 \u201c20% \u8840\u6e05 DMEM\u201d\uff0c\u4e0e NCI-H358 \u7684 \u201c10% \u8840\u6e05 1640\u201d \u4e00\u81f4\uff08\u53ef\u5171\u7528\u57fa\u7840\u57f9\u517b\u57fa\u964d\u4f4e\u6210\u672c\uff09\uff1b\u8840\u6e05\u9009\u62e9\u4f4e\u5185\u6bd2\u7d20\u80ce\u725b\u8840\u6e05\uff08\u226410EU\/mL\uff09\uff0c\u65e0\u9700\u50cf SRA01\/04 \u90a3\u6837\u4f7f\u7528\u9ad8\u8840\u6e05\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c1640 \u57f9\u517b\u57fa\u65e0\u9700\u907f\u5149\uff0c\u64cd\u4f5c\u4fbf\u6377\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u57f9\u517b\u6761\u4ef6<\/b>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff08\u4e0e SRA01\/04\u3001NCI-H358 \u57f9\u517b\u73af\u5883\u53c2\u6570\u4e00\u81f4\uff0c\u65e0\u9700\u989d\u5916\u8c03\u6574\uff1b\u60ac\u6d6e\u7ec6\u80de\u5bf9\u6c14\u4f53\u4ea4\u6362\u654f\u611f\uff0c\u57f9\u517b\u74f6\u9700\u4fdd\u7559 1\/3 \u7a7a\u95f4\uff08\u907f\u514d\u5bc6\u5c01\uff09\uff0c\u786e\u4fdd\u6c14\u4f53\u6d41\u901a\uff1b\u56e0\u589e\u6b96\u5feb\u4e14\u8425\u517b\u6d88\u8017\u5747\u5300\uff0c\u9700\u6bcf 48 \u5c0f\u65f6\u66f4\u6362 1 \u6b21\u57f9\u517b\u57fa\uff08\u79bb\u5fc3\u6536\u96c6\u7ec6\u80de\u540e\u91cd\u60ac\u4e8e\u65b0\u9c9c\u57f9\u517b\u57fa\uff09\uff0c\u65e0\u9700\u50cf\u8d34\u58c1\u7ec6\u80de\u90a3\u6837\u62c5\u5fc3\u6d88\u5316\u635f\u4f24\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" data-line=\"true\" data-list=\"bullet1\"><b>\u51bb\u5b58\u6761\u4ef6<\/b>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\uff0c\u6db2\u6c2e\u50a8\u5b58\uff083\u00d710\u2076cells\/mL\uff0c\u9ad8\u4e8e\u8d34\u58c1\u7ec6\u80de\u5bc6\u5ea6\uff1b4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\u2192\u6db2\u6c2e\u50a8\u5b58\uff1b\u51bb\u5b58\u524d\u9700\u786e\u4fdd\u7ec6\u80de\u56e2\u7c07\u5206\u6563\uff08\u8f7b\u8f7b\u5439\u6253\u81f3\u5c0f\u56e2\u7c07\uff09\uff0c\u6dfb\u52a0 10% FBS \u53ef\u63d0\u5347\u590d\u82cf\u540e\u56e2\u7c07\u91cd\u7ec4\u80fd\u529b\uff0c\u907f\u514d\u51bb\u5b58\u65f6\u5927\u56e2\u7c07\u4e2d\u5fc3\u7ec6\u80de\u574f\u6b7b\uff1b\u590d\u82cf\u65f6\u5feb\u901f\u6c34\u6d74\uff0837\u2103\uff0c1 \u5206\u949f\u5185\uff09\u6eb6\u89e3\uff0c\u76f4\u63a5\u52a0\u5165\u65b0\u9c9c\u57f9\u517b\u57fa\uff08\u65e0\u9700\u79bb\u5fc3\uff09\uff0c\u51cf\u5c11\u5bf9\u60ac\u6d6e\u7ec6\u80de\u7684\u635f\u4f24\uff09<\/li>\n<\/ul>\n<ul>\n<li class=\"temp-li bullet1\" 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