{"id":341,"date":"2025-08-29T22:49:56","date_gmt":"2025-08-29T14:49:56","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=341"},"modified":"2025-08-29T22:49:56","modified_gmt":"2025-08-29T14:49:56","slug":"tj905%e4%ba%ba%e8%83%b6%e8%b4%a8%e7%98%a4%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/tj905%e4%ba%ba%e8%83%b6%e8%b4%a8%e7%98%a4%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"TJ905\u4eba\u80f6\u8d28\u7624\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<h2 class=\"header-vfC6AV auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u7ec6\u80de\u540d\u79f0<\/strong>\uff1aTJ905 \u4eba\u80f6\u8d28\u7624\u7ec6\u80de<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6838\u5fc3\u7279\u8272\uff1a\u8111\u9876\u53f6\u80f6\u8d28\u7624\uff08\u8111\u6765\u6e90\uff09+ \u8d34\u58c1\u751f\u957f + \u591a\u89d2\u7ec6\u80de\u6837\u5f62\u6001 + DMEM+10% FBS+PS \u57f9\u517b\u57fa +~41.03 \u5c0f\u65f6\u500d\u589e + GFAP\uff08\u80f6\u8d28\u7ea4\u7ef4\u9178\u6027\u86cb\u767d\uff09\/S-100 \u86cb\u767d \/ \u6ce2\u5f62\u86cb\u767d\u9633\u6027 + \u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u57fa\u7840\u533b\u5b66\u7814\u7a76\u6240\u7ec6\u80de\u8d44\u6e90\u4e2d\u5fc3\u4fdd\u85cf\uff0c\u53ef\u4e0e U2OS\uff08\u9aa8\u8089\u7624 \/ \u8d34\u58c1 \/ McCoy&#8217;s 5a \/ \u77ed\u500d\u589e\uff09\u3001U266\uff08\u591a\u53d1\u6027\u9aa8\u9ad3\u7624 \/ \u60ac\u6d6e \/ RPMI-1640 \/ \u5206\u6ccc IL-6\uff09\u7ec4\u6210\u5bf9\u6bd4\u4f53\u7cfb\uff0c\u7528\u4e8e\u8111\u80f6\u8d28\u7624\u673a\u5236\u3001\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u80bf\u7624 vs \u5176\u4ed6\u80bf\u7624\uff08\u9aa8 \/ \u8840\u6db2\uff09\u5dee\u5f02\u3001\u80f6\u8d28\u7ec6\u80de\u7279\u5f02\u6027\u86cb\u767d\uff08GFAP\uff09\u529f\u80fd\u53ca\u8d34\u58c1\u8111\u80bf\u7624\u7ec6\u80de\u57f9\u517b\u7814\u7a76\u3002<\/li>\n<li><strong>\u7ec6\u80de\u522b\u79f0<\/strong>\uff1aTj-905\uff1b\u4eba\u80f6\u8d28\u7624\u7ec6\u80de<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6807\u6ce8 \u201c\u8111\u80f6\u8d28\u7624\uff08\u9876\u53f6\u6765\u6e90\uff09+ \u8d34\u58c1 + \u591a\u89d2\u7ec6\u80de\u6837 + DMEM+GFAP \u9633\u6027\u201d\uff0c\u533a\u522b\u4e8e\u9aa8\u6765\u6e90\u9aa8\u8089\u7624\uff08U2OS\uff09\u3001\u60ac\u6d6e\u8840\u6db2\u80bf\u7624\uff08U266\uff09\uff0c\u89c4\u907f\u80bf\u7624\u90e8\u4f4d\u53ca\u57f9\u517b\u64cd\u4f5c\u6df7\u6dc6\u3002<\/li>\n<li><strong>\u79cd\u5c5e\u6765\u6e90<\/strong>\uff1a\u4eba\uff08\u65e0\u4ea4\u53c9\u6c61\u67d3\uff0c\u79cd\u5c5e\u7eaf\u51c0\uff0c\u4fdd\u969c\u80f6\u8d28\u7624\u8d34\u58c1\u80fd\u529b\u3001\u591a\u89d2\u5f62\u6001\u3001DMEM \u57f9\u517b\u57fa\u9002\u5e94\u53ca GFAP\/S-100 \u86cb\u767d\u8868\u8fbe\u7a33\u5b9a\u6027\uff0c\u9002\u914d\u8111\u80f6\u8d28\u7624\u57fa\u7840\u7814\u7a76\u3001\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u80bf\u7624\u5b9e\u9a8c\u573a\u666f\uff09<\/li>\n<li><strong>\u7ec4\u7ec7\u6765\u6e90<\/strong>\uff1a\u8111\uff08\u9876\u53f6\u80f6\u8d28\u7624\uff09\uff0c\u4fdd\u7559\u8111\u80f6\u8d28\u7624\u7279\u6709\u7684\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u80bf\u7624\u7279\u5f81\uff0c\u6838\u5fc3\u4f18\u52bf\u4e3a \u201c\u8111\u539f\u53d1 + \u80f6\u8d28\u7ec6\u80de\u7279\u5f02\u6027\u86cb\u767d\u9633\u6027 + \u660e\u786e\u500d\u589e\u201d\uff0c\u9002\u914d\u8111\u80f6\u8d28\u7624\u5fae\u73af\u5883\u4ea4\u4e92\u3001\u80f6\u8d28\u7ec6\u80de\u5206\u5316\u673a\u5236\u53ca\u8111\u80bf\u7624\u836f\u7269\u7b5b\u9009\u5b9e\u9a8c\u3002<\/li>\n<li><strong>\u751f\u957f\u7279\u6027<\/strong>\uff1a\u8d34\u58c1\u751f\u957f\uff08\u7eaf\u8d34\u58c1\uff0c\u64cd\u4f5c\u7b80\u4fbf\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>0.25% \u80f0\u9176 37\u2103\u5b75\u80b2 4-6 \u5206\u949f\u6d88\u5316\uff08\u591a\u89d2\u7ec6\u80de\u8d34\u58c1\u4e2d\u7b49\u7d27\u5bc6\uff0c\u6d88\u5316\u65f6\u95f4\u4ecb\u4e8e U2OS \u4e0e U266\uff08\u60ac\u6d6e\u65e0\u9700\u6d88\u5316\uff09\u4e4b\u95f4\uff09\uff0c\u6c47\u5408\u5ea6 80%-90% \u65f6\u6309 1:2-1:3 \u4f20\u4ee3\uff0c24 \u5c0f\u65f6\u5185\u8d34\u58c1\u7a33\u5b9a\uff0c\u9002\u5408\u5f00\u5c55\u8111\u80f6\u8d28\u7624\u7279\u5f02\u6027\u86cb\u767d\u68c0\u6d4b\u53ca\u836f\u7269\u654f\u611f\u6027\u5b9e\u9a8c\u3002<\/li>\n<li><strong>\u7ec6\u80de\u5f62\u6001<\/strong>\uff1a\u591a\u89d2\u7ec6\u80de\u6837\uff08\u7ec6\u80de\u5448\u4e0d\u89c4\u5219\u591a\u89d2\u5f62\uff0c\u80de\u8d28\u4e30\u5bcc\uff0c\u8fb9\u754c\u6e05\u6670\uff0c\u6392\u5217\u677e\u6563\uff0c\u4e0e U2OS \u7684\u4e0a\u76ae\u7ec6\u80de\u6837\uff08\u9aa8\u8089\u7624\uff09\u3001U266 \u7684\u6dcb\u5df4\u6bcd\u7ec6\u80de\u6837\uff08\u9aa8\u9ad3\u7624\uff09\u5f62\u6001\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7 \u201c\u8111\u80f6\u8d28\u7624\u6765\u6e90 + GFAP \u9633\u6027\u201d \u5feb\u901f\u9274\u522b\uff0c\u9002\u914d\u8111\u80f6\u8d28\u7624\u5f62\u6001\u5b66\u9a8c\u8bc1\u53ca\u80f6\u8d28\u7ec6\u80de\u7279\u5f81\u5173\u8054\u5b9e\u9a8c\uff09<\/li>\n<li><strong>\u80cc\u666f\u7b80\u4ecb<\/strong>\uff1a\u4eba\u8111\u9876\u53f6\u80f6\u8d28\u7624\u7ec6\u80de\u7cfb\uff0c\u7eaf\u8d34\u58c1\u751f\u957f\u5448\u591a\u89d2\u7ec6\u80de\u6837\uff0cDMEM+10% FBS \u57f9\u517b\u57fa\u53ef\u7a33\u5b9a\u57f9\u517b\uff1b\u5177\uff5e41.03 \u5c0f\u65f6\u500d\u589e\uff0cGFAP\u3001S-100 \u86cb\u767d\u3001\u6ce2\u5f62\u86cb\u767d\u9633\u6027\uff08\u80f6\u8d28\u7ec6\u80de\u7279\u5f02\u6027\u6807\u5fd7\u7269\uff09\uff1b\u65e0\u9aa8\u8089\u7624\u7684 IGF \u53d7\u4f53\u8868\u8fbe\u6216\u9aa8\u9ad3\u7624\u7684 IL-6 \u5206\u6ccc\u7279\u6027\uff0c\u662f\u7814\u7a76\u8111\u80f6\u8d28\u7624\u3001\u80f6\u8d28\u7ec6\u80de\u529f\u80fd\u53ca\u4e2d\u67a2\u795e\u7ecf\u7cfb\u7edf\u80bf\u7624\u7684\u7406\u60f3\u6a21\u578b\uff0c\u53ef\u4e0e\u8111\u6b63\u5e38\u80f6\u8d28\u7ec6\u80de\u5bf9\u6bd4\u63a2\u7d22\u80bf\u7624\u7ec6\u80de\u4e0e\u6b63\u5e38\u7ec6\u80de\u7684\u5dee\u5f02\u3002<\/li>\n<li><strong>STR \u4f4d\u70b9\u4fe1\u606f<\/strong>\uff1aAmelogenin\uff1aX\uff0cY\uff1bCSF1PO\uff1a11\uff0c12\uff1bD13S317\uff1a13\uff1bD16S539\uff1a9\uff0c11\uff1bD18S51\uff1a13\uff0c15\uff1bD19S433\uff1a15.2\uff0c16\uff1bD21S11\uff1a30\uff1bD2S1338\uff1a22\uff0c25\uff1bD3S1358\uff1a17\uff1bD5S818\uff1a11\uff0c12\uff1bD7S820\uff1a11\uff0c12\uff1bD8S1179\uff1a12\uff0c14\uff1bFGA\uff1a25\uff1bTH01\uff1a7\uff0c9.3\uff1bTPOX\uff1a8\uff0c11\uff1bvWA\uff1a16\uff0c18\uff08\u53ef\u4e0e\u540c\u7c7b\u8111\u80bf\u7624\u7ec6\u80de STR \u56fe\u8c31\u5bf9\u6bd4\uff0c\u786e\u8ba4\u80f6\u8d28\u7624\u8eab\u4efd\uff0c\u6392\u9664\u5176\u4ed6\u90e8\u4f4d\u80bf\u7624\u7ec6\u80de\u6c61\u67d3\uff09<\/li>\n<li><strong>\u7ec6\u80de\u89c4\u683c<\/strong>\uff1a1\u00d710\u2076cells\/T25 \u8d34\u58c1\u57f9\u517b\u74f6\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226585%\uff0c24 \u5c0f\u65f6\u8d34\u58c1\u7387\u226590%\uff0c\u9700\u901a\u8fc7\u514d\u75ab\u8367\u5149\u68c0\u6d4b GFAP \u9633\u6027\u7387\uff08\u226585%\uff09\uff0c\u786e\u8ba4\u80f6\u8d28\u7ec6\u80de\u7279\u5f81\u3002<\/li>\n<li><strong>\u652f\u539f\u4f53\u68c0\u6d4b<\/strong>\uff1a\u65e0\uff08\u5efa\u8bae\u6bcf 2 \u4ee3 PCR \u590d\u6d4b\uff0c\u907f\u514d\u6c61\u67d3\u5f71\u54cd\u8111\u80f6\u8d28\u7624\u7279\u5f02\u6027\u86cb\u767d\u8868\u8fbe\u53ca\u836f\u7269\u5b9e\u9a8c\u7ed3\u679c\uff09<\/li>\n<li><strong>\u4fdd\u85cf\u673a\u6784<\/strong>\uff1a\u4e2d\u56fd\u533b\u5b66\u79d1\u5b66\u9662\u57fa\u7840\u533b\u5b66\u7814\u7a76\u6240\u7ec6\u80de\u8d44\u6e90\u4e2d\u5fc3\uff08\u56fd\u5185\u6743\u5a01\u673a\u6784\u4fdd\u85cf\uff0c\u80f6\u8d28\u7624\u5c5e\u6027\u53ca STR \u9274\u5b9a\u7ed3\u679c\u7ecf\u9a8c\u8bc1\uff0c\u53ef\u8ffd\u6eaf\u6027\u5f3a\uff0c\u9002\u914d\u56fd\u5185\u8111\u80bf\u7624\u7814\u7a76\u5b9e\u9a8c\u7684\u7ec6\u80de\u6765\u6e90\u5408\u89c4\u6027\u8981\u6c42\uff09<\/li>\n<li><strong>\u57f9\u517b\u57fa<\/strong>\uff1aDMEM+10% FBS+PS\uff08\u8840\u6e05\u9009\u4f4e\u5185\u6bd2\u7d20\u226410EU\/mL\uff0c\u5f00\u5c01\u540e 2 \u5468\u5185\u7528\u5b8c\uff0c\u4fdd\u969c\u591a\u89d2\u5f62\u6001\u53ca GFAP\/S-100 \u86cb\u767d\u8868\u8fbe\u7a33\u5b9a\uff0c\u533a\u522b\u4e8e U2OS \u7684 McCoy&#8217;s 5a\u3001U266 \u7684 RPMI-1640\uff0c\u4e0d\u53ef\u6df7\u7528\u57f9\u517b\u57fa\uff09<\/li>\n<li><strong>\u57f9\u517b\u6761\u4ef6<\/strong>\uff1a95% \u7a7a\u6c14 + 5% \u4e8c\u6c27\u5316\u78b3\uff0c37\u2103\uff0c\u6e7f\u5ea6 70%-80%\uff0c\u6bcf 48 \u5c0f\u65f6\u6362\u6db2 1 \u6b21\uff08\u6cbf\u74f6\u58c1\u7f13\u6162\u52a0\u6db2\uff0c\u907f\u514d\u51b2\u51fb\u8d34\u58c1\u7684\u591a\u89d2\u7ec6\u80de\uff09\uff1b\u57f9\u517b\u7bb1\u6e29\u5ea6\u6ce2\u52a8\u9700\u2264\u00b10.5\u2103\uff0c\u9632\u6b62\u6e29\u5ea6\u53d8\u5316\u5f71\u54cd\u80f6\u8d28\u7ec6\u80de\u7279\u5f02\u6027\u86cb\u767d\u8868\u8fbe\u3002<\/li>\n<li><strong>\u51bb\u5b58\u6761\u4ef6<\/strong>\uff1a\u65e0\u8840\u6e05\u51bb\u5b58\u6db2\u6db2\u6c2e\u50a8\u5b58\uff082\u00d710\u2076cells\/mL\uff09\uff0c4\u2103\u5e73\u8861 30 \u5206\u949f\u2192-20\u2103 2 \u5c0f\u65f6\u2192-80\u2103\u8fc7\u591c\uff1b\u51bb\u5b58\u524d\u9700\u786e\u4fdd\u7ec6\u80de\u5904\u4e8e\u5bf9\u6570\u751f\u957f\u671f\uff08GFAP 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