{"id":342,"date":"2025-08-29T22:52:02","date_gmt":"2025-08-29T14:52:02","guid":{"rendered":"https:\/\/www.fengyaosw.com\/?post_type=product&#038;p=342"},"modified":"2025-08-29T22:52:03","modified_gmt":"2025-08-29T14:52:03","slug":"thp-1%e4%ba%ba%e5%8d%95%e6%a0%b8%e7%bb%86%e8%83%9e%e7%99%bd%e8%a1%80%e7%97%85%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89","status":"publish","type":"product","link":"https:\/\/www.fengyaosw.com\/index.php\/product\/thp-1%e4%ba%ba%e5%8d%95%e6%a0%b8%e7%bb%86%e8%83%9e%e7%99%bd%e8%a1%80%e7%97%85%e7%bb%86%e8%83%9e%ef%bc%88str%e9%89%b4%e5%ae%9a%e6%ad%a3%e7%a1%ae%ef%bc%89\/","title":{"rendered":"THP-1\u4eba\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u7ec6\u80de\uff08STR\u9274\u5b9a\u6b63\u786e\uff09"},"content":{"rendered":"<h2 class=\"header-vfC6AV auto-hide-last-sibling-br\">\u4e00\u3001\u7ec6\u80de\u57fa\u672c\u5c5e\u6027\uff08\u5ba2\u670d\uff1a\u5c0f\u70fd\uff1a18030101858\uff09<\/h2>\n<ul class=\"auto-hide-last-sibling-br\">\n<li><strong>\u7ec6\u80de\u540d\u79f0<\/strong>\uff1aTHP-1 \u4eba\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u7ec6\u80de\uff08STR \u9274\u5b9a\u6b63\u786e\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6838\u5fc3\u7279\u8272\uff1a\u6025\u6027\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\uff08\u5916\u5468\u8840\u6765\u6e90\uff0cSTR \u9274\u5b9a\u6b63\u786e\uff09+ \u60ac\u6d6e\u751f\u957f + \u5355\u6838\u7ec6\u80de\u5f62\u6001 + 90%1640+10% FBS+PS+0.05mM 2 &#8211; \u5def\u57fa\u4e59\u9187\uff08\u5fc5\u9700\u6210\u5206\uff09\u590d\u5408\u57f9\u517b\u57fa +~36-72 \u5c0f\u65f6\u500d\u589e + PMA \u53ef\u8bf1\u5bfc\u5411\u5355\u6838\u7cfb\u5206\u5316 + \u5177\u541e\u566c\u529f\u80fd\uff08\u4e73\u80f6\u9897\u7c92 \/ \u6fc0\u6d3b\u7ea2\u7ec6\u80de\uff09+ \u67d3\u8272\u4f53 80~84\/48~52 + \u591a\u673a\u6784\u4fdd\u85cf\uff08ATCC; TIB-202 \u7b49\uff09\uff0c\u53ef\u4e0e TJ905\uff08\u8111\u80f6\u8d28\u7624 \/ \u8d34\u58c1 \/ DMEM\/GFAP \u9633\u6027\uff09\u3001U266\uff08\u591a\u53d1\u6027\u9aa8\u9ad3\u7624 \/ \u60ac\u6d6e \/ 15% \u8840\u6e05 \/ \u5206\u6ccc IL-6\uff09\u7ec4\u6210\u5bf9\u6bd4\u4f53\u7cfb\uff0c\u7528\u4e8e\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u673a\u5236\u3001\u8840\u6db2\u80bf\u7624\u4e9a\u578b\uff08\u5355\u6838\u7ec6\u80de\u578b vs \u6d46\u7ec6\u80de\u578b\uff09\u5dee\u5f02\u3001PMA \u8bf1\u5bfc\u5206\u5316\u53ca 2 &#8211; \u5def\u57fa\u4e59\u9187\u4f9d\u8d56\u578b\u7ec6\u80de\u57f9\u517b\u7814\u7a76\u3002<\/li>\n<li><strong>\u7ec6\u80de\u522b\u79f0<\/strong>\uff1aTHP1\uff1b\u4eba\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u7ec6\u80de<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u6807\u6ce8 \u201c\u6025\u6027\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5 + \u60ac\u6d6e + 1640+2 &#8211; 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\u5def\u57fa\u4e59\u9187\u5fc5\u9700 + \u541e\u566c\u6d3b\u6027\u201d\uff0c\u9002\u914d\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u53d1\u75c5\u673a\u5236\u3001\u708e\u75c7\u76f8\u5173\u5b9e\u9a8c\u53ca\u7ec6\u80de\u5206\u5316\u7814\u7a76\u3002<\/li>\n<li><strong>\u751f\u957f\u7279\u6027<\/strong>\uff1a\u60ac\u6d6e\u751f\u957f\uff08\u7eaf\u60ac\u6d6e\uff0c\u64cd\u4f5c\u4e0e\u8d34\u58c1\u7ec6\u80de\u5dee\u5f02\u663e\u8457\uff09<br class=\"container-utlnW2 wrapper-d0Cc1k undefined\" \/>\u65e0\u9700\u80f0\u9176\u6d88\u5316\uff0c\u76f4\u63a5\u79bb\u5fc3\uff081000rpm\uff0c5 \u5206\u949f\uff09\u6536\u96c6\u4f20\u4ee3\uff0c\u7ec6\u80de\u5bc6\u5ea6\u8fbe 3\u00d710\u2075-6\u00d710\u2075cells\/mL \u65f6\u6309 1:2 \u4f20\u4ee3\uff1bPMA \u8bf1\u5bfc\u65f6\uff08\u7ec8\u6d53\u5ea6 10-20ng\/mL\uff09\uff0c24-48 \u5c0f\u65f6\u53ef\u5411\u5355\u6838\u7cfb\u5206\u5316\uff08\u5f62\u6001\u53d8\u68ad\u5f62\u3001\u9ecf\u9644\u80fd\u529b\u589e\u5f3a\uff09\uff1b\u5e38\u89c4\u57f9\u517b\u6bcf 12 \u5c0f\u65f6\u8f7b\u8f7b\u6447\u5300 1 \u6b21\uff0c\u907f\u514d\u7ec6\u80de\u6c89\u964d\u805a\u96c6\uff0c\u9002\u5408\u5f00\u5c55\u541e\u566c\u5b9e\u9a8c\u53ca\u5206\u5316\u76f8\u5173\u7814\u7a76\u3002<\/li>\n<li><strong>\u7ec6\u80de\u5f62\u6001<\/strong>\uff1a\u5355\u6838\u7ec6\u80de\u6837\uff08\u5448\u5706\u5f62\u6216\u7c7b\u5706\u5f62\uff0c\u80de\u4f53\u4e2d\u7b49\u5927\u5c0f\uff0c\u80de\u8d28\u542b\u7ec6\u5c0f\u9897\u7c92\uff0c\u4e0e TJ905 \u7684\u591a\u89d2\u7ec6\u80de\u6837\uff08\u8d34\u58c1\u80f6\u8d28\u7624\uff09\u3001U266 \u7684\u6dcb\u5df4\u6bcd\u7ec6\u80de\u6837\uff08\u9aa8\u9ad3\u7624\uff09\u5f62\u6001\u5dee\u5f02\u663e\u8457\uff0c\u53ef\u901a\u8fc7 \u201c\u60ac\u6d6e + \u5355\u6838\u5f62\u6001 + PMA \u8bf1\u5bfc\u5206\u5316\u201d \u5feb\u901f\u9274\u522b\uff0c\u9002\u914d\u5355\u6838\u7ec6\u80de\u5f62\u6001\u5b66\u89c2\u5bdf\u53ca\u5206\u5316\u72b6\u6001\u8bc4\u4f30\u5b9e\u9a8c\uff09<\/li>\n<li><strong>\u80cc\u666f\u7b80\u4ecb<\/strong>\uff1a\u4eba\u6025\u6027\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u7ec6\u80de\u7cfb\uff08STR \u9274\u5b9a\u6b63\u786e\uff09\uff0c\u7eaf\u60ac\u6d6e\u751f\u957f\u5448\u5355\u6838\u7ec6\u80de\u6837\uff0c\u542b 0.05mM 2 &#8211; \u5def\u57fa\u4e59\u9187\u7684 1640 \u590d\u5408\u57f9\u517b\u57fa\u53ef\u7a33\u5b9a\u57f9\u517b\uff1b\u5177\u541e\u566c\u4e73\u80f6\u9897\u7c92 \/ \u6fc0\u6d3b\u7ea2\u7ec6\u80de\u80fd\u529b\uff0cC3R\u3001FcR \u9633\u6027\uff0c\u65e0\u514d\u75ab\u7403\u86cb\u767d\u8868\u8fbe\uff0cPMA \u53ef\u8bf1\u5bfc\u5411\u5355\u6838\u7cfb\u5206\u5316\uff0c\u53ef\u4f5c\u4e3a\u8f6c\u67d3\u5bbf\u4e3b\uff1b\u65e0\u80f6\u8d28\u7624\u7684 GFAP \u8868\u8fbe\u6216\u9aa8\u9ad3\u7624\u7684 IL-6 \u5206\u6ccc\u7279\u6027\uff0c\u662f\u7814\u7a76\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u3001\u7ec6\u80de\u5206\u5316\u53ca\u708e\u75c7\u673a\u5236\u7684\u7406\u60f3\u6a21\u578b\uff0c\u53ef\u4e0e PMA \u8bf1\u5bfc\u540e\u7ec6\u80de\u5bf9\u6bd4\u63a2\u7d22\u5206\u5316\u5bf9\u529f\u80fd\u7684\u5f71\u54cd\u3002<\/li>\n<li><strong>STR \u4f4d\u70b9\u4fe1\u606f<\/strong>\uff1aAmelogenin\uff1aX\uff0cY\uff1bCSF1PO\uff1a11\uff0c13\uff1bD13S317\uff1a8\uff0c13\uff1bD16S539\uff1a11\uff0c12\uff1bD18S51\uff1a13\uff0c14\uff0c15\uff1bD19S433\uff1a12.2\uff0c13\uff0c14\uff1bD21S11\uff1a29\uff0c30\uff0c31.2\uff1bD2S1338\uff1a17\uff0c18\uff1bD3S1358\uff1a15\uff0c16\uff0c17\uff1bD5S818\uff1a11\uff0c12\uff1bD7S820\uff1a10\uff1bD8S1179\uff1a10\uff0c14\uff1bFGA\uff1a22\uff0c24\uff0c25\uff1bTH01\uff1a7\uff0c8\uff0c9.3\uff1bTPOX\uff1a8\uff0c11\uff1bvWA\uff1a16\uff08\u53ef\u4e0e\u540c\u7c7b\u8840\u6db2\u80bf\u7624\u7ec6\u80de STR \u56fe\u8c31\u5bf9\u6bd4\uff0c\u786e\u8ba4\u5355\u6838\u7ec6\u80de\u767d\u8840\u75c5\u8eab\u4efd\uff0c\u6392\u9664\u5b9e\u4f53\u7624\u6216\u5176\u4ed6\u767d\u8840\u75c5\u4e9a\u578b\u6c61\u67d3\uff09<\/li>\n<li><strong>\u7ec6\u80de\u89c4\u683c<\/strong>\uff1a1\u00d710\u2076cells\/T25 \u57f9\u517b\u74f6\uff08\u60ac\u6d6e\u72b6\u6001\uff0c\u542b\u57f9\u517b\u6db2\uff09\u6216 1mL \u51bb\u5b58\u7ba1\uff0c\u590d\u82cf\u540e\u5b58\u6d3b\u7387\u226580%\uff0c24 \u5c0f\u65f6\u5185\u6062\u590d\u60ac\u6d6e\u5747\u4e00\u6027\uff0c\u9700\u901a\u8fc7\u663e\u5fae\u955c\u89c2\u5bdf\u5355\u6838\u7ec6\u80de\u5f62\u6001\u5360\u6bd4\uff08\u226590%\uff09\u3002<\/li>\n<li><strong>\u652f\u539f\u4f53\u68c0\u6d4b<\/strong>\uff1a\u65e0\uff08\u5efa\u8bae\u6bcf 2 \u4ee3 PCR \u590d\u6d4b\uff0c\u60ac\u6d6e\u7ec6\u80de\u6c61\u67d3\u6613\u6269\u6563\uff0c\u907f\u514d\u5f71\u54cd\u541e\u566c\u529f\u80fd\u53ca PMA \u8bf1\u5bfc\u5206\u5316\u5b9e\u9a8c\u7ed3\u679c\uff09<\/li>\n<li><strong>\u4fdd\u85cf\u673a\u6784<\/strong>\uff1aATCC; 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